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Tytuł:
Występowanie i morfologia niektórych drapieżnych grzybów pełzako-, wrotko- i niecieniobójczych w wodach powierzchniowych okolic Białegostoku
Autorzy:
Kiziewicz, B.
Czeczuga, B.
Powiązania:
https://bibliotekanauki.pl/articles/2147658.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
okolice Bialegostoku
wystepowanie
morfologia grzybow
grzyby wodne
wody powierzchniowe
grzyby drapiezne
Źródło:
Wiadomości Parazytologiczne; 2003, 49, 3; 281-291
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kontaminacja grzybami drożdżopodobnymi produktów mleczarskich
Autorzy:
Białasiewicz, D.
Kłosiński, M.
Powiązania:
https://bibliotekanauki.pl/articles/2147953.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przetwory mleczne
parazytologia
grzyby drozdzoidalne
zanieczyszczenia mikrobiologiczne
zanieczyszczenia zywnosci
kontaminacja
grzyby chorobotworcze
Opis:
Evaluation of the degree of contamination of diary products with potentially pathogenic fungal strains. Materials for the study were: yoghurt, cottage cheese, homogenised cottage cheese, cream and buttermilk. Differentiation of species was based on their morphological features and biochemical properties (API 20 C bioMerieux). Among 300 studied diary products we have isolated 100 (33,0±2,72%) strains of yeast-like fungi. Diary products were contamined with 19 species of fungi from types: Candida, Geotrichum, Trichosporon, Saccharomyces and Rhodotorula. The highest percentage of fungi was seen in cream (95,7±4,23%), cottage cheese (87,9±5,68%) and kefir (60,0±15,5%). In other studied products the frequency ranged from 13, 1±2,54% to 35,2±6,51 %. Among the isolated fungi the following species prevailed: Geotrichum candidum (18,0±3,84%), Candida lusitaniae (15,0±3,57%) and C. inconspicua (11,0±3,13%).
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 3; 345-351
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Poszukiwanie grzybów potencjalnie chorobotwórczych dla człowieka w wodach Jeziora Charzykowskiego w Zaborskim Parku Krajobrazowym
Potentially pathogenic fungi in the waters of the Charzykowskie Lake in Zaborski Landscape Park
Autorzy:
Kurnatowski, P.
Rózga, A.
Rózga, B.
Babski, P.
Wójcik, A.
Powiązania:
https://bibliotekanauki.pl/articles/2143852.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zagrozenia czlowieka
zagrozenia zdrowia
Jezioro Charzykowskie
Zaborski Park Krajobrazowy
wody jeziorne
grzyby wodne
grzyby chorobotworcze
Opis:
The occurrence of potentially pathogenic fungal strains in the Charzykowskie Lake and runnels flowing into and out of it was investigated. The study material was obtained in 2005 and in 2006, in the periods of spring intermix and summer stagnation, and in 2005 in the period of autumn intermix. The fungi found in the Charzykowski Lake belonged to 5 genera: Rhodotorula (R. minuta, R.rubra and R. glutinis), Cryptoccocus (C. neoformans, C. laurentii, C. terreus and C. laurentii), Candida (C. inconspicua, C. lusitaniae, C. tropicalis, C. pelliculosa, C. kefir, C. glabrata, C. inconspicua, C. parapsilosis, C. ciferrii and C. colliculosa), Trichosporon (T. cutaneum) and Klockera (K. apiculata). The fungi found in runnels flowing into and out of the Charzykowskie Lake belonged to 4 genera: Rhodotorula (R. rubra and R. glutinis), Cryptoccocus (C. laurentii, C. neoformans, C. albidus and C. terreus), Candida (C. colliculosa, C. lusitaniae, C. tropicalis, C. pelliculosa, C.cifferii, C. glabrata) and Trichosporon (T. cutaneum).
Źródło:
Wiadomości Parazytologiczne; 2007, 53, 2; 109-115
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie technik molekularnych do wykrywania i/lub identyfikacji pasożytów i grzybów u ludzi i zwierząt oraz patogenów przenoszonych przez kleszcze. Cz.2
ZASTOSOWANIE TECHNIK MOLEKULARNYCH DO WYKRYWANIA I/LUB IDENTYFIKACJI PASOŻYTÓW I GRZYBÓW U LUDZI I ZWIERZĄT ORAZ PATOGENÓW PRZENOSZONYCH PRZEZ KLESZCZE. CZĘŚĆ
Autorzy:
Budak, A.
Gołąb, E.
Majewska, A.C.
Wędrychowicz, H.
Bajer, A.
Siński, E.
Myjak, P.
Stańczak, J.
Powiązania:
https://bibliotekanauki.pl/articles/2148020.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka molekularna
grzyby pasozytnicze
patogeny grzybowe
dermatofity
parazytologia
techniki badawcze
grzyby drozdzoidalne
metody molekularne
Pneumocystis carinii
identyfikacja
wykrywanie
grzyby chorobotworcze
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 3; 457-463
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antagonistyczne interakcje między grzybami saprotroficznymi a geohelmintami. 2. Grzyby saprotroficzne w bioregulacji pasożytniczych geohelmintów człowieka i zwierząt
Antagonistic interactions between saprotrophic fungi and geohelminths. 2. Saprotrophic fungi in biocontrol of parasitic geohelminths of humans and animals
Autorzy:
Jaborowska-Jarmoluk, M.
Mazurkiewicz-Zapałowicz, K.
Kołodziejczyk, L.
Powiązania:
https://bibliotekanauki.pl/articles/2143485.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zwalczanie pasozytow
interakcje nicienie-grzyby
grzyby glebowe
oddzialywania antagonistyczne
pasozyty zwierzat
bioregulacja
geohelminty
nicienie pasozytnicze
nicienie glebowe
grzyby saprotroficzne
pasozyty czlowieka
Opis:
The soils ecosystem plays an important role in the epidemiology of geohelminth diseases of humans and animals. Soil contamination with ova of the parasitic geohelminths represents a global public health-hazard issue. Biological agents have been thought to control the infective forms of parasites present in the soil. Biocontrol of geohelminths represents an alternative to pesticides (i.e., nematicides), which are not efficient in killing infective nematode forms and, additionally, result in the environment pollution and long-term disturbances in the soil ecosystem homeostasis. The degree of the inhibiting effect of soil saprotrophic fungi on geohelminth embryonic development varies and depends on the species. A number of fungi cause various morphological disorders in the embryos of developing parasitic nematodes, but also have an ovicidal effect. Although the nature of the antagonism between fungi and other living organisms has not been fully explained, it is certain that mycotoxins and fungal enzymes constitute its important components. Considering the studies carried out so far, the antagonistic effect of mold fungi against the infective stages of geohelminths can be fully recommended as a real control factor, especially as these saprotrophs represent a natural factor within the soil environment, that is of particular biochemical activity.
Źródło:
Wiadomości Parazytologiczne; 2009, 55, 1; 9-17
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Drapieżne grzyby nicieniobójcze
Autorzy:
Czygier, M.
Boguś, M.I.
Powiązania:
https://bibliotekanauki.pl/articles/2147832.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty zwierzat
nicienie zoladkowo-jelitowe
parazytologia
grzyby nicieniobojcze
zwalczanie pasozytow zwierzat
grzyby drapiezne
Opis:
Gastrointestinal nematodes are considered a serious economic problem affecting the livestock industry around the world. Current methods of their control, relaying mainly on organic drugs, are not sustainable because parasites develop resistance to anthelmintic and bacause of increasing public concem about chemicals residues in livestock products and environment. Nematode-trapping fungi offer a very promissing, nonchemotherapeutic approach to nematode parasite control. Their potential in preventing nematodosis is well documented. In this paper we outline the present knowlege on mechanisms involved in trapping and killing nematodes by the predacious nematode-destroying fungi.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 1; 25-31
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aspekty ekologiczne występowania Trichosporon cutaneum [de Beurman Gougerot et Vaucher, 1909 Ota, 1915] w wodach północno-wschodniej Polski
ASPECTS OF ECOLOGICAL OCCURRENCES TRICHOSPORON CUTANEUM (DE BEURMAN GOUGEROT ET VAUCHER, 1909 OTA, 1915) IN WATERS NORTH-EAST POLANDS
Autorzy:
Kiziewicz, B.
Czeczuga, B.
Powiązania:
https://bibliotekanauki.pl/articles/2148216.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Polska Polnocno-Wschodnia
wystepowanie
aspekty ekologiczne
Trichosporon cutaneum
grzyby wodne
wody powierzchniowe
grzyby chorobotworcze
Opis:
The aim of work was settlement of occurrence of pathogenic fungus Trichosporon cutaneum in diverse ecological environments. Object to investigations farmer river Węgorapa, Supraśl, lakes of complex Mamry and Sejny group, of cultivation ponds in Popielewo and in Poryte Jabłoń a few springs of city Białystok. In investigations one used method of baits to isolated water fungus. Quality of waters determined by physico-chemistry methods. One fixed, that pathogcnic fungus Trichosporon cutaneum stepped out in waters north-east evn Poland on all investigated sites. It was developed in both strong poluted water of lakes and ponds also in good oxygened waters and springs it was too.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 4; 783-788
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Grzyby u chorych na cukrzycę typu 2: prewalencja, gatunki wyizolowanych szczepów
Fungi in patients with diabetes mellitus type 2: prevalence, species of isolated strains.
Autorzy:
Maciejewska, A.
Jaskółowska, A.
Kwaśniewska, J.
Powiązania:
https://bibliotekanauki.pl/articles/2146446.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
gatunki grzybow
grzyby
choroby czlowieka
cukrzyca
szczepy grzybow
Opis:
In 77 diabetic patients the detection of fungi was carried out in the oral cavity, digestive tract, genital organs and skin lesions. Patient age ranged from 39 to 82 years, with the Body Mass Index (BMI) from 22 to the highest values of 46.5. Diabetes control was assessed by determination of the glucose level in serum after overnight fasting and after lunch, as well as by determination of the НБА | с level. Fungi were diagnosed in 61 patients (79.6%). The total of 111 fungal strains were isolated in 319 cultures evaluated from 12 biological materials. Fungal strains were detected in the highest percentage in oral cavity (77.9%), in the lower percentage in anus (33.8%), vagina discharge (11.6%) and the region of vulva (14.3%). Fungi were observed in one focus — exclusively in the oral cavity — in 28 (36.4%) patients, whereas they occurred in multifocal infections in 33 (42.9%) patients. The isolated fungal strains were classified into 4 genera (Candida, Saccharomyces, Trichosporon, Aspergillus) and 12 species. The most frequently detected fungi were C. albicans (55.2%), followed by C. glabrata (12.4%), C parapsilosis (10.5%) and С. tropicalis (9.5%). Relatively low percentage of fungi identified in vagina was associated, among others, with the fact that most of the female patients with diabetes were at the postmenopausal period. In all the patients in whom fungi were isolated from the vagina contents, these fungi were also found in other ontocenoses. The increase in percentage of fungal strains from genera other than Candida in patients with a higher risk is of clinical importance, as some of them are resistant in vitro to azole derivatives e.g. fluconazole. The digestive tract is thought to be the most frequent fungal habitat. A considerable prevalence of fungi, multifocal infections, particularly related to oral cavity and anus, and occurrence of high percentage of strains different from C. albicans species of Candida genus in female patients with type 2 diabetes indicate the necessity of the special mode of diagnostic and therapeutic management.
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 2; 163-170
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Grzyby drożdżopodobne - występowanie u zwierząt
Yeast-like fungi — occurrence in animals
Autorzy:
Dworecka-Kaszak, B.
Biegańska, M.
Powiązania:
https://bibliotekanauki.pl/articles/2147584.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
kandydoza
wystepowanie
Candida
parazytologia
grzyby drozdzoidalne
choroby zwierzat
Opis:
The yeast-like fungi are typical opportunistic microorganisms which may be a part of normal commensally ontocenosis. Many species of Candida genus may be also a cause of candidosis in human and animals. Clinical symptoms occurred customly in patient with cellular immunity deficiencies. Horizontal (person-to-person) form of transmission is also possible, including family infection or group infection in animals, mostly in birds. According to actual data, zoonotic status of candidosis is still not fully documented. In animals, candidosis more often occurs as endogenic infection. The separate problem is infection of animals with significance suppressed immune system, e.g. with tumor diseases. In necroscopy investigation very often in these animals disseminated candidosis has been observed. On different way the infections of Candida spp. are manifested in birds, when Candida albicans occurs as marginal population of normal intestinal ontocenosis. During stress the intestinal fungal population is growing up for critical value and typical crop candidosis is developing. In our routine diagnostic work we have isolated Candida strains from nutritional tract in laboratory mice. This yeast were isolated also from the bottom of the boxes and from mice faeces.
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 4; 707-713
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stężenie zarodników grzybów alergogennych w powietrzu mieszkań w Łodzi
CONCENTRATION OF ALLERGIC FUNGI SPORES IN THE AIR OF FLATS IN ŁÓDŹ
Autorzy:
Krawczyk, P.
Kowalski, M. L.
Ochecka-Szymanska, A.
Powiązania:
https://bibliotekanauki.pl/articles/2148765.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
alergeny
grzyby
mieszkania
Lodz
stezenie
powietrze wewnetrzne
zarodniki
alergologia
Opis:
The real contribution of moulds to the pathogenesis of allergic diseases remains unknown, although positive skin prick tests andlor spesific serum IgE to moki allergens can be detected in 1-5% of atopic patients. A significant problem in assesment of exposure to mould allergens, resulting with difficulty in standarization of methods. The aim of this work was to assess the concentration of spores of 8 mould species in flats inhabited by peoples who dont show any symptoms of allergy. The Open Petri Dish (OPD) method involving sedimentation of participles contained in the column of air ower the dish was used to assess the number of spores in 1m3 of indoor atmospheres. All colonies were counted, but only 8 mould species implicated in inhaled allergy were identified, ie.: Alternaria tenuis, Cladosporium herbarum, Helminthosporum halodes, Pullularia pullulans, Penicillium notatam, Rhizopus nigricans, Mucor mucedo, Aspergillus fumigatus. The tests were carried out in 10 flats located in various quarters of the cify of Łódź during three consecutive days of September 1995 between 5:00 pm and 6:04 pm. In analyzing the percentage of spores of each of the eight mould species tested we determined that, independent of fiat and test day, C. herbarum predominated. It is good agreement with the observations of other authors who report that among large quantities of fungi that are detected in late summer, usually C. herbarum spores dominate. This is the season when the incidence of the Cladosporium spores in the atmospheric air increases. Spores of H. halodes were detected least frequently. Our study demonstrated the presence of substantial amounts of mould spores in indoor air of houses in Łódź. The spores belong to species with documented allergenicity, suggesting that they may play a role in development of allergic sensitization in susceptible subjects.
Źródło:
Wiadomości Parazytologiczne; 1999, 45, 2; 255-262
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena niektórych danych klinicznych u pacjentów z łupieżem pstrym
Evaluation of some clinic data in patients with tinea versicolor
Autorzy:
Kwaśniewska, J.
Moruś-Urbańska, E.
Jaskółowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/2143476.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Malassezia
grzybice
grzyby
lupiez
lupiez pstry
ocena kliniczna
pacjenci
Opis:
Fungi from Malassezia species may be a component of human and animal skin onthocenosis but also may cause general mycoses in patients from high risk groups. The aim of study was the evaluation of some clinic data in patients with tinea versicolor, who entered to Department of Biology and Medical Parasitology, Centre for Treatment of Parasitic Diseases and Mycoses, Medical University of Lodz. Forty four patients with tinea versicolor were examined. Localization and number of skin lesions as well as eventually risk factor were examined. In all patients mycological examine was done; morphotic elements of fungi in direct and stained slides were found, cultures on solid medium with oil olive were done. In Wood light fluorescence of changed skin was observed. The most incidence of tinea versicolor was observed in young persons to compare with to another age groups. The most frequently localization of clinical lesions concerned back skin, back and chest. In women – to compare with to male patients – clinical lesions on back skin were significantly more frequent. The only one factor was conducived to infection – it was a seborrhea of the skin, observed mainly in patients from the youngest age group.
Źródło:
Wiadomości Parazytologiczne; 2010, 56, 4; 323-327
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Grzyby wodne wystepujące w zbiorniku wodnym w Zarzeczanach w województwie podlaskim
Water fungi occurrence in the water reservoir in Zarzeczany of Podlasie province
Autorzy:
Kiziewicz, B.
Powiązania:
https://bibliotekanauki.pl/articles/2147453.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
woj.podlaskie
wies Zarzeczany
zbiorniki wodne
wystepowanie
grzyby wodne
Opis:
Studies on the occurrence of aquatic microorganisms in the newest water reservoir Zarzeczany village were carried out in years 2001-2004. Hydro chemical analysis was performed using standard methods. Bait method was used to isolate the fungi. In the reservoir 52 fungi species were identified: fish pathogenic Achlya debaryana, A. polyandra, Aphanomyces laevis, Dictyuchus monosporus, D. sterile, Saprolegnia ferax, S. monoica, S. parasitica, human pathogenic Candida albicans, C. tropicalis and Trichosporon cutaneum, decomposing keratin Blastocladiopsis parva, Catenaria anguillulae, C. verrucosa, Catenophlyctis variabilis, Lagenidium humanum, Rhizophydium keratinophilum, and decomposing chitin Aphanomyces astaci, Karlingia chitinophila, Rhizidium chitinophilum, Asterophlyctis irregularis and Blastocladiella brytanica.
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 3; 587-593
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Charakterystyka enzymów proteo-, chityno- i lipolitycznych pasożytniczego grzyba Conidiobolus coronatus
Characterization of proteo-, chitino- and lipolytic enzymes of parasitic fungus Conidiobolus coronatus
Autorzy:
Włóka, E
Powiązania:
https://bibliotekanauki.pl/articles/2143413.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
grzyby pasozytnicze
grzyby entomopatogenne
Conidiobolus coronatus
Entomophthorales
enzymy proteolityczne
elastaza
N-acetyloglukozaminidaza
enzymy chitynolityczne
enzymy lipolityczne
lipaza
aktywnosc enzymatyczna
owady
barciak wiekszy
Galleria mellonella
zwalczanie szkodnikow
metody biologiczne
grzyby owadobojcze
Opis:
The largest problem in limitation of insect pest population is increasing resistance of them to chemical pesticides. Alternative are entomopathogens, which regulate frequency of insect pests. Among them decisive role play entomopathogenic fungi, which possess the ability to active penetration through cuticle by mechanical pressure of invasive hypha and production of proteo-, chitino- (egzo- and endochitinases) as well as lipolytic enzymes, which provide nutrients for subsequent development of fungus. Entomopathogenic soil fungus Conidiobolus coronatus (Entomophtorales) is saprophyte fungus, which demonstrates a high efficiency in the paralysis of varied insects. Although leading investigations over mechanism of insect paralysis, we still do not know, what role fungal enzymes play in insect cuticle penetration. The main aim of research was establishment of optimal conditions for elastase, N-acetylglucosaminidase (NAGase), chitobiosidase as well as lipase. Optimal reaction parameters were determined: volume of reaction mixture, volume of homogenate, working pH and the substrate concentration. Having on aim a possible use of C. coronatus in pest control, two ranges of temperatures were chosen: 20°C – optimal temperature for the fungus growing and 30°C – optimal temperature for the cultivation of the great wax moth larvae, Galleria mellonella, on which examinations were performed. Also kinetic constants Km and Vmax were determined. Activity of elastase and N-acetylglucosaminidase of C. coronatus was measured spectrophotometrically at 410 nm (towards N-Succinyl-Ala-Ala-Pro-Leu-p-Nitroanilide) and 405 nm (towards 4-Nitrophenyl-N-acetyl-b-D-glucosaminide), respectively. The following optimal conditions of elastase activity were established: the volume of reaction mixture 0.5 ml, volume of homogenate 1 ml, temperature 30°C, pH 8, substrate concentration 40 mM. Optimal conditions of NAGase assay: the volume of reaction mixture 0.5 ml, dose of homogenate 12.5 ml, temperature 30°C, pH neutral and 6 mM substrate concentration. The activities of chitobiosidase and lipase were measured spectrofluorometrically (Ex=360 nm, Em=450 nm) towards 4-Methylumbelliferyl b-D-N-N’-diacetylchitobioside and 4-Methylumbelliferyl oleate, respectively. Chitobiosidase showed the highest activity in dose of 30 ml in 1 ml volume of reaction mixture, at the temperature of 30°C, pH 7 and substrate concentration equal to 2 mM. Lipase showed the highest catalytic activity in 1 ml volume of reaction mixture, in 30°C but 50 ml of homogenate, pH 10 and 10 mM substrate concentration were needed. Higher activity investigated enzymes in 30°C than 20°C indicated that they can take part in pathogenesis. It was suggested that as first in perforation of coats of insects body elastase and lipase take part. Indicated of it, large thermoresistance of both enzymes (only 10.5% decrease of elastase activity at 20°C and 9.4% decrease of lipase activity in comparison with maximal activity at 30°C), alkalophilicity of both proteins (elastase shows the alkaline optimal pH equal to 8 at pH 9 preserves 97% activity, and at pH 10 94% activity, respectively while lipase prefers the pH 10 and at pH 8 and pH 9 enzyme keeps 57 and 60% activity, respectively) as well as lack of repression by suitable substrates. Sigmoid character of curve concerning pH influence on the activity of both enzymes, also indicates similarity between elastase and lipase. On minor part of NAGase and chitobiosidase of fungus C. coronatus in perforation of coats of host body showed high sensibility of both enzymes on hydrogen ions concentration: both enzymes prefer neutral pH, in pH 6 and 8 lose over 35% activity but subjection to substrate repression and 3–4-fold growth of activity followed only in 30°C. In the course of work it was found, that rich medium (LB) stimulates growth of mycelium and production of fungal lipases. So far nobody managed to isolate chitinolytic or lipolytic enzymes from C. coronatus homogenate. The majority of fungal enzymes were isolated from post incubation filtrates. In the literature of the subject lack of data about C. coronatus NAGase, therefore in examinations also the trial of isolation NAGase from C. coronatus homogenate was undertaken. Activity of NAGase showed only first fraction, which did not separate with none of used columns. Disappointing results of purification on cation exchanger CM, weak anion exchanger DEAE, and strong anion exchanger Q were obtained as well as after fractionation tests with the use of Microcon microcolumns. In aim of NAGase molecular mass estimation, two zymograms were made with Triton X-100 and casein and with the use of fluorescent substrate 4-Methylumbelliferyl N-acetyl-b-D-glucosaminide. Molecular mass of NAGase from C. coronatus was established on ca. 60 kDa. This is the first report describing molecular weight of NAGase from C. coronatus. Examined NAGase has different properties than known NAGases from other entomopathogenic fungi. Although its molecular weight is equal to the Metarhizium anisopliae NAGase, optimal pH for both NAGases are different: neutral in the case of C. coronatus NAGase versus acidic in the case of M. anisopliae NAGase. Knowledge of molecular mass of the C. coronatus NAGase should allow to find a new method of this enzyme isolation from C. coronatus homogenate. Thanks to developed methods of assaying activities of elastase, NAGase, chitobiosidase and lipase, real becomes the understanding of mechanism of insects paralysis through C. coronatus fungus.
Źródło:
Wiadomości Parazytologiczne; 2010, 56, 1; 83-85
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Grzyby jako czynnik etiologiczny chorób górnych dróg oddechowych
Fungi as an aetiological factor of upper respiratory diseases.
Autorzy:
Kurnatowski, P.
Raczyńska-Witońska, G.
Powiązania:
https://bibliotekanauki.pl/articles/2146445.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
grzyby
czynniki chorobotworcze
choroby gornych drog oddechowych
etiologia
Opis:
Mycologic investigations were carried out in subjects suffering from upper respiratory tract ailments. The material for assessment was collected from various ontocenoses. Species of fungal strains were determined by means of the morphological and biochemical analyses. Special attention was paid to the frequency of fungi occurring in multiple foci of the same system. In patients presented with ailments of the upper respiratory tract the presence of fungi, predominantly species of Candida genus was detected in different ontocenoses. In 1/4 of cases the fungi were found in 2 or 3 foci, which can induce systemic transmission, interfere with treatment and result in recurrent infections.
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 2; 157-162
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania in vitro lekoodporności szczepów grzybów z rodzaju Candida
IN VITRO RESISTANCE OF CANDIDA SPECIES TO ANTIFUNGAL AGENTS
Autorzy:
Banach-Piątkowska, W.
Kotłowski, A.
Humanowska, J.
Mayer, L.
Kowalczyk, D.
Powiązania:
https://bibliotekanauki.pl/articles/2148224.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
lekoopornosc
grzyby
szczepy chorobotworcze
Candida
leki przeciwgrzybicze
badania in vitro
Opis:
The purpose of this study was to determine the in vitro susceptibility to 6 antifungal agents of 143 strains of Candida species isolated from 545 patients with suspicion of fungal infection. Test ATB Fungus (bioMerieux) was used. Among all Candida isolates 91.6% were susceptible to tested polyenes and 32.9% to all azoles. Less susceptible to polyenes isolates were noted in low percentage (0.7 to 4.2%) and to azoles more frequcntly (32.9 to 41.2%). Candida isolates resistant to antifungal agents tested were dependent from the kind of clinical samples and species of fungi. Monitoring of the susceptibility to antifungal agents by ATB commercial test seems to be uscful tool for therapeutic purposes.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 4; 811-816
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
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