Autor: Werner, C. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Genotypowanie izolatów Giardia duodenalis uzyskanych od ludzi w zachodnio-centralnej Polsce
Genotype analysis of Giardia duodenalis isolated obtained from humans in West-central Poland
Autorzy:: Solarczyk, P.
Werner, A.
Majewska, A.C.
Powiązania:: https://bibliotekanauki.pl/articles/2143436.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: Giardia duodenalis
genotypowanie
genotyp
pasozyty jelitowe
pasozyty czlowieka
Polska Srodkowo-Zachodnia
Opis:: Giardia duodenalis (syn. G. intestinalis, G. lamblia) is a cosmopolitan flagellate organism belonging to the most common intestinal protozoan parasites of humans and animals. Great genetic heterogeneity has been found within G. duodenalis, where only genotypes representing assemblages A and B have zoonotic potential. Fecal samples (447 specimens) obtained from 232 humans in West-central region of Poland were examined by microscopy and PCR. The total prevalence of Giardia in humans was 1.3%. DNA was extracted from three positive fecal samples and PCR products were obtained after amplification using the β-giardin primers G7 and G759. Sequence and phylogenetic analyses showed that G. duodenalis isolates from humans belonged to A and B genotypes. Moreover, three subgenotypes, including a cosmopolitan subgenotype A2 and two new subgenotypes A and B were detected.
Źródło:: Wiadomości Parazytologiczne; 2010, 56, 2; 171-177
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Ocena użyteczności metod wykrywania Cryptosporidium w kale ludzi i zwierząt
Evaluation of usefulness of different methods for detection of Cryptosporidium in human and animal stool samples
Autorzy:: Werner, A.
Sulima, P.
Majewska, A.C.
Powiązania:: https://bibliotekanauki.pl/articles/2146455.pdf
Data publikacji:: 2004
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: ocena przydatnosci
kal
metody wykrywania
zwierzeta
Cryptosporidium
czlowiek
Opis:: There are many methods for detection of Cryptosporidium oocysts. Most of them (more than 20) enable the microscopic detection of Cryptosporidium oocysts in faecal smears. Such a great variability of diagnostic methods may lead to confusion as far as the choice of an appropriate technique by a given laboratory is concerned. This study evaluated the diagnostic usefulness of Cryptosporidium oocysts and coproantigen detection methods in the diagnosis of cryptosporidiosis in human (266 stool specimen) and animals (205 from cattle, 160 from sheep, 30 from horses, 80 from cats, 227 from dogs and 11 from wild animals). The total number of human and animal stool specimens processed was 266 and 713, respectively. In this study the usefulness of several diagnostic methods was compared. The following techniques were taken into account: wet mounts, hematoxylin staining, four different specific methods (modified Zeihl-Neelsen, Kinyoun's, safraninmethylene blue, as well as carbol-methyl violet and tartrazyne) and commercially available kit based on enzyme-linked immunoassay (ProspecT(r) Cryptosporidium Microplate Assay). The final number of positive specimens was 123. Out of them 77 were positive in all specific methods. The oocysts found in stool specimens were measured. Humans were infected with C. parvum and animals with C. parvum, C. andersoni or C. felis. The statistical analysis has shown that EIA test was a better than microscopy method for identification of Cryptosporidium in faecal samples in human and wild animal. Sensitivity and specificity are important factors for the choice of a proper diagnostic method for Cryptosporidium detection, however other factors such as cost, simplicity and ease of interpretation of results are also important considerations.
Źródło:: Wiadomości Parazytologiczne; 2004, 50, 2; 209-220
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Kryptosporydioza u osób zakażonych HIV
Autorzy:: Majewska, A. C.
Sulima, P.
Werner, A.
Barałkiewicz, G.
Juszczyk, J.
Pieniążek, N.J.
Powiązania:: https://bibliotekanauki.pl/articles/2148749.pdf
Data publikacji:: 1999
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: kryptosporydioza
choroby czlowieka
wirus HIV
parazytologia
Opis:: Cryptosporidium parvum is a coccidian parasite that affects millions of people worldwide. Clinical outcome of human cryptosporidiosis differs between immunocompetent and immunodeficient individuals. C. parvum is responsible for causing protracted and life-threatening diarrhea, biliary, and pulmonary infections in immunocompromised persons, especially in patients with AIDS. Though no effective treatment has been found so far, early diagnosis may be useful in controlling the infection. Thirty-eight stool specimens obtained from 35 HIV-positive patients admitted to the Clinic of Infectious Diseases in Poznań, Poland, were examined for the detection of oocysts, coproantigen and DNA of Cryptosporidium using standard microscopic, immunologic and molecular diagnostic methods. The presence of Cryptosporidium was detected in 10 HIV-positive patients. Oocysts, coproantigen and DNA of this parasite were identified solely in one specimen while Cryptosporidium DNA was detected in 8 specimens. Cryptosporidium coproantigen was found only in one sample. Although, the PCR was the most useful technique in the detection of Cryptosporidium in HIV-positive patients it should be noted that PCR has many pitfalls and needs to be carefully controlled to avoid both false positive and false negative results.
Źródło:: Wiadomości Parazytologiczne; 1999, 45, 2; 125-128
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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