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    Wyświetlanie 1-4 z 4
    Tytuł:
    Badania roli receptorów Toll-podobnych [TLR] w odpowiedzi żywiciela na inwazje pasożytnicza na tle współczesnej wiedzy o TLR u ssaków i modelowego nicienia Caenorhabditis elegans
    The investigations of the role of toll-like receptors [TLR] in host response to parasitic infection of current back-ground regarding TLR in mammals and the model nematode Caenorhabditis elegans
    Autorzy:
    Wojtkowiak, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143925.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    odpowiedz immunologiczna
    receptory toll-podobne
    ssaki
    parazytologia
    organizmy modelowe
    Caenorhabditis elegans
    nicienie
    inwazja pasozytnicza
    Opis:
    Toll−like receptors (TLRs) are amongst the most highly conserved in the evolution of receptor family, being found in both immune and other cells. TLRs were observed in vascular endothelial cells, epithelial cells, microglia cells, adipocytes, and intestinal and renal cells. TLRs plays a key role in the innate immune response to a variety of pathogens. At present, very little is known about the role of TLRs in host defense against parasitic pathogen infections. The first study shows that TLRs contribute to both innate and adaptive immune responses following infection with protozoan parasite Leishmania major. The TLRs recognizing PAMPs associated with the parasite L. major are essential for the activation of the innate and adaptive immune responses to infection. A study concerning recognition of the role of TLRs in the host−parasite relationship would be an interesting challenge for future study.
    Źródło:
    Wiadomości Parazytologiczne; 2007, 53, 3; 203-211
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Badania in vitro wpływu albendazolu na kinetykę reakcji katalizowanej przez cytozolową transferazę glutationową [GST] z wątroby szczura
    Effect in vitro of albendazole on the kinetics of cytosolic glutathione transferase from the rat liver
    Autorzy:
    Wojtkowiak, A.
    Boczoń, K.
    Wandurska-Nowak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143851.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    szczury
    katalizatory
    kinetyka reakcji enzymatycznych
    albendazol
    transferaza S-glutationowa
    watroba
    transferazy glutationowe
    badania in vitro
    Opis:
    Introduction. Since the idea of multifunctional mode of action of anthelmintics is considered and in experimental trichinellosis in vivo albendazole seems to act as an allosteric activator of cytosolic GST from mice muscles, in this study a termosensitivity after in vitro incubation with albendazole of purified commercial cytosolic glutathione transferase (GST) from the rat liver was investigated. Methods. Two extremal temperatures: −80°C and +30°C were used to destroy the dimer in quaternary structure of this enzyme. Results. In control preparations both extremal temperatures destroy this structure, so the Michaelis−Menten kinetic curves of substrate saturation show the typical hyperbolic shape. After a long (15 h) freezing at −80°C or heating (up to 14 h at +30°C) the kinetics of substrate saturation of GST after incubation with albendazole show the sigmoidal or "double sigmoidal" shape, pointing out the quaternary GST structure as a complex of "frozen subunits". Drug inhibits about 6−times the total activity of GST after incubation at +30°C. We conclude that albendazole in vitro influences the structure of cytosolic GST from the rat liver and inhibits its activity, but, in opposite to in vivo study in mouse muscles infected with Trichinella spiralis larvae, does not act as an activator of this enzyme.
    Źródło:
    Wiadomości Parazytologiczne; 2007, 53, 2; 97-102
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ albendazolu na cytoplazmatycznę transferazę-S-glutationową i ekspresję receptorów Toll-podobnych w jelitowej fazie włośnicy
    The influence of albendazole on the cystolic glutatione-S-transferase [GST] and on the expression of Toll-like receptors [TLR2 and TLR4] during the intestinal phase of trichinellosis
    Autorzy:
    Wojtkowiak-Giera, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143438.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    choroby pasozytnicze
    wlosnica
    faza jelitowa
    enzymy detoksykacyjne
    transferaza S-glutationowa
    receptory Toll-podobne
    ekspresja receptorow
    leki przeciwpasozytnicze
    albendazol
    Opis:
    Research into changes in the activity and kinetics of the reaction catalysed by the important detoxificating enzyme, glutathione-S-transferase (GST), in the intestinal phase of trichinellosis as presented in the present study is a pioneering achievement. In every instance of infection – or if infection is only suspected – the patient receives anthelmintics that are effective against mature intestinal forms, newborn larvae and encysted larvae of Trichinella spiralis. This group of drugs includes benzimidazoles such as albendazole. The primary action of these drugs concerns β-tubuline. The functions of microtubules are damaged by bonding β-tubuline with benzimidazoles, which inhibits the polymerisation of microtubules. Newly considered fields of action of benzimidazoles on the pathological changes occurring in the host during the course of parasitic diseases are the following elements taking part in the immunological defence against a parasitic infection: for example, detoxificating enzymes such as glutathione-Stransferase (GST), and Toll-like receptors (TLRs). Occurrence of cell resistance to drugs is attributed to certain enzymes, (such as GST), which perform the detoxification of these drugs and facilitate their removal from cells through a pump dependent on compounds coupled with glutathione. In the case of the intestinal phase of trichinellosis, GST may remove toxins secreted by both the adult and newborn larvae of T. spiralis. The structure of a dimeric enzyme of the host, such as GST, may be impacted by various factors, for example excretory-secretory products (ES), as well as drugs used. Over the past few years, the interest of researchers in the innate immune response as the first line of defense of vertebrates against of pathogens, was increased. The number of works devoted to the role of Toll-like receptors, which enable the immediate commencement of protective action by the host against various infections without engaging lymphocytes, is on the noticeable. The experimental material consisted of small and large intestine from BALB/c mice infected with T. spiralis strain MSUS/PO/60/ISS3. Mice were inoculated orally with about 400 T. spiralis larvae per mouse. On the 2nd day post-infection, the mice were treated with albendazole, in a dose of 0.5 mg/mouse. The activity and kinetics of the GST were determined spectrophotometrically. An evaluation of the expression of TLRs (TLR2 and TLR4 on the mRNA level) was examined using the RT-PCR technique with the appropriate starter sequences for TLR2 and TLR4. The presence of Toll-like receptors and their expression on the protein level was confirmed using the immunohistochemical method (Santa Cruz ABC Stainig system sc-2023 test) with specific polyclonal antibodies antiTLR2 and anti-TLR4. My results pointed to the participation of GST, as well as Toll-like receptors, in defence processes of the host during the intestinal phase of trichinellosis in mice. The adult and newborn larvae of T. spiralis present in the intestines of infected mice are a source of excretory-secretory (ES) products, which appear to stimulate the activity and change catalytic properties of conformers GST. It is supposed that ES products secreted by the parasite acts on GST as an allosteric activator. Additionally, the presence of Toll-like receptors (TLR2 and TLR4) in the intestinal phase of trichinellosis was investigated. Thus, the infection with T. spiralis leads to strong biochemical reactions with the involvement of GST, and immunological reactions with the involvement of TLR2 and TLR4 receptors. Administration of albendazole, resulted in 2-fold decrease of the total activity of GST in comparison with its activity in infected and untreated animals. Also, drug administration had weakened expression of gene of TLR4 and TLR2 in the large intestine, and expression of gene of TLR2 in the small intestine. My results confirmed destructively action of albendazole on T. spiralis (resulted in 4-fold decrease in the intensity of infection measured in muscles). The destruction by the drug of a adult and newborn larvae of T. spiralis lowers the quantities of their ES products stimulated the activation of the host’s response. This is apparent as weakening the innate immune response, in which take part Toll-like receptors play a role, and in biochemical processes with the participation of the GST enzyme. In summary my results contribute new data on the participation of Toll-like receptors and GST in the defence mechanisms of the host during in the intestinal phase of experimental trichinellosis in mice.
    Źródło:
    Wiadomości Parazytologiczne; 2010, 56, 2; 181-183
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Intrygujący wpływ albendazolu na aktywność reduktazy glutationu w mięśniach myszy zarażonych Trichinella spiralis
    The intriguing influence of albendazole on glutathione reductase activity in the muscles from Trichinella spiralis infected mice.
    Autorzy:
    Przydanek, A.
    Boczoń, K.
    Wandurska-Nowak, E.
    Wojt, W.
    Wojtkowiak, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146462.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    aktywnosc enzymatyczna
    Trichinella spiralis
    miesnie
    parazytologia
    albendazol
    myszy
    reduktaza glutationowa
    infekcja pasozytnicza
    Opis:
    A clear stimulation of the activity of glutathione reductase (RG, EC 1.6.4.2), one of the major enzymes participating in glutathione metabolism, was observed in the skeletal muscles from Trichinella spiralis infected mice. The maximal, almost twofold increase in RG activity was observed in week 5 post-infection. Administration of albendazole, an anthelmintic widely used in trichinellosis treatment, resulted in an additional, about 40% stimulation of RG activity (small, but statistically significant) on week 2 post-infection. In weeks 6 and 7 postinfection, when no stimulation of RG activity in infected mice caused by drug was observed, the drug modified the structure of that allosteric enzyme, affecting the kinetics of its substrate saturation in this way that the shape of the substrate saturation curve changed from „double sigmoidal”, typical of this enzyme kinetics in the muscles from infected mice, to hyperbolic, typical of this enzyme kinetics in the control, uninfected animals.
    Źródło:
    Wiadomości Parazytologiczne; 2004, 50, 2; 273-277
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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