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    Wyświetlanie 1-4 z 4
    Tytuł:
    Zastosowanie technik biologii molekularnej w parazytologii: za i przeciw
    APPLICATION OF MOLECULAR BIOLOGY TECHNIQUES IN PARASITOLOGY: FOR AND AGAINST
    Autorzy:
    Majewska, A. C.
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148868.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parazytologia
    techniki badawcze
    techniki biologii molekularnej
    diagnostyka
    Opis:
    This review deals with possible advantages and disadvantages connected with applying molecular biology techniques in parasitology. It particularly concerns molecular diagnosis and epidemiology, as well as phylogenesis and taxonomy of selected parasite species.
    Źródło:
    Wiadomości Parazytologiczne; 1998, 44, 2; 181-194
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Źródło i znaczenie genetycznego zróżnicowania populacji pasożytniczych pierwotniaków
    SOURCE AND SIGNIFICANCE OF GENETIC POLYMORPHISM OF SELECTED PARASITIC PROTOZOA
    Autorzy:
    Majewska, A. C.
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148795.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    zroznicowanie genetyczne
    Leishmania
    Giardia
    struktura populacji
    pierwotniaki
    parazytologia
    zroznicowanie gatunkowe
    Toxoplasma
    populacje zwierzat
    pasozyty
    Trypanosoma
    Cryptosporidium
    Opis:
    The application of biochemical and molecular techniques in parasitological studies has provided increasing evidences of genetic polymorphism among parasite populations. This review presents possible origins of genetic variation within populations of various protozoan species. Since the mode of reproduction has an important influence on genetic polymorphism within parasite populations these considerations refer mainly to some protozoan parasites which have various life cycles, e.g. Giardia, Trypanosoma, Cryptosporidium, Toxoplasma. Also other factors associated with parasites (such as: transmission and passage history in laboratory conditions; occurrence in different hosts or geographic regions; selective pressure of drugs; competitive interactions between populations) that affect parasite genetic diversity are discussed. However, the number of examined isolates of parasites and genetic markers, assortment of methods, probes, primers and reagents used is also of significance. The significance of genetic variability in parasite populations is still the subject of much interest and controversy. A simple interpretation of such variation is impossible because of the complexity of host-parasite interactions. The knowledge of parasite diversity at the nucleic acids level has continually increased, but a corect interpretation of this phenomenon requires at least the same knowledge of genetic variability in host populations. Nevertheless, genetic variability in protozoan parasites has many important implications, e.g. for taxonomy, epidemiology, control and evolution. Genetic differences within parasite populations might also be associated with phenotypic variability, e.g. virulence, antigenicity, infectivity, drug sensitivity, hostpreference etc.
    Źródło:
    Wiadomości Parazytologiczne; 1999, 45, 3; 293-307
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena użyteczności metod wykrywania Cryptosporidium w kale ludzi i zwierząt
    Evaluation of usefulness of different methods for detection of Cryptosporidium in human and animal stool samples
    Autorzy:
    Werner, A.
    Sulima, P.
    Majewska, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146455.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    ocena przydatnosci
    kal
    metody wykrywania
    zwierzeta
    Cryptosporidium
    czlowiek
    Opis:
    There are many methods for detection of Cryptosporidium oocysts. Most of them (more than 20) enable the microscopic detection of Cryptosporidium oocysts in faecal smears. Such a great variability of diagnostic methods may lead to confusion as far as the choice of an appropriate technique by a given laboratory is concerned. This study evaluated the diagnostic usefulness of Cryptosporidium oocysts and coproantigen detection methods in the diagnosis of cryptosporidiosis in human (266 stool specimen) and animals (205 from cattle, 160 from sheep, 30 from horses, 80 from cats, 227 from dogs and 11 from wild animals). The total number of human and animal stool specimens processed was 266 and 713, respectively. In this study the usefulness of several diagnostic methods was compared. The following techniques were taken into account: wet mounts, hematoxylin staining, four different specific methods (modified Zeihl-Neelsen, Kinyoun's, safraninmethylene blue, as well as carbol-methyl violet and tartrazyne) and commercially available kit based on enzyme-linked immunoassay (ProspecT(r) Cryptosporidium Microplate Assay). The final number of positive specimens was 123. Out of them 77 were positive in all specific methods. The oocysts found in stool specimens were measured. Humans were infected with C. parvum and animals with C. parvum, C. andersoni or C. felis. The statistical analysis has shown that EIA test was a better than microscopy method for identification of Cryptosporidium in faecal samples in human and wild animal. Sensitivity and specificity are important factors for the choice of a proper diagnostic method for Cryptosporidium detection, however other factors such as cost, simplicity and ease of interpretation of results are also important considerations.
    Źródło:
    Wiadomości Parazytologiczne; 2004, 50, 2; 209-220
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Kryptosporydioza u osób zakażonych HIV
    Autorzy:
    Majewska, A. C.
    Sulima, P.
    Werner, A.
    Barałkiewicz, G.
    Juszczyk, J.
    Pieniążek, N.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148749.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    kryptosporydioza
    choroby czlowieka
    wirus HIV
    parazytologia
    Opis:
    Cryptosporidium parvum is a coccidian parasite that affects millions of people worldwide. Clinical outcome of human cryptosporidiosis differs between immunocompetent and immunodeficient individuals. C. parvum is responsible for causing protracted and life-threatening diarrhea, biliary, and pulmonary infections in immunocompromised persons, especially in patients with AIDS. Though no effective treatment has been found so far, early diagnosis may be useful in controlling the infection. Thirty-eight stool specimens obtained from 35 HIV-positive patients admitted to the Clinic of Infectious Diseases in Poznań, Poland, were examined for the detection of oocysts, coproantigen and DNA of Cryptosporidium using standard microscopic, immunologic and molecular diagnostic methods. The presence of Cryptosporidium was detected in 10 HIV-positive patients. Oocysts, coproantigen and DNA of this parasite were identified solely in one specimen while Cryptosporidium DNA was detected in 8 specimens. Cryptosporidium coproantigen was found only in one sample. Although, the PCR was the most useful technique in the detection of Cryptosporidium in HIV-positive patients it should be noted that PCR has many pitfalls and needs to be carefully controlled to avoid both false positive and false negative results.
    Źródło:
    Wiadomości Parazytologiczne; 1999, 45, 2; 125-128
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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