- Tytuł:
-
Zastosowanie spektroskopii oscylacyjnej w zakresie nir do śledzenia modyfikacji konformacyjnych zachodzących w hydrofobowym obszarze modelowych dwuwarstw lipidowych
Application of nir range vibrational spectroskopy to follow the conformational modifications occuring in the hydrophobic region of model lipid bilayers - Autorzy:
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Kuć, Marta
Cieślik-Boczula, Katarzyna
Rospenk, Maria - Powiązania:
- https://bibliotekanauki.pl/articles/27310038.pdf
- Data publikacji:
- 2023
- Wydawca:
- Polskie Towarzystwo Chemiczne
- Tematy:
-
dwuwarstwa lipidowa
liposom
główne przejście fazowe
spektroskopia oscylacyjna w zakresie średniej podczerwieni (MIR)
spektroskopia oscylacyjna w zakresie bliskiej podczerwieni (NIR)
Analiza Składników Głównych (PCA)
lipid bilayer
liposome
main phase transition
Mid-infrared vibrational spectroscopy (MIR)
Near-infrared vibrational spectroscopy (NIR)
Principal Component Analysis (PCA) - Opis:
- Biomembranes, which are the structural and functional basis of the cells of all living organisms, have been an extremely interesting research object for biology and chemistry scientists for years. The multitude of elements constituting the components of natural lipid membranes, however, is associated with interpretation difficulties regarding the nature of the processes taking place in them. A useful research object that is a model of bilamellar biosystems with a significantly simplified composition and at the same time retaining properties that can be a reference point in relation to natural membranes are lipid membranes in the form of one or several component liposomes. It is precisely such systems built of molecules of dipalmitoyl phosphatidylcholine (DPPC) or dipalmitoyl phosphatidylglycerol (DPPG), and analogous systems with the addition of cholesterol (Chol), that were the subject of research in this work. Near-infrared (NIR) vibrational spectroscopy provides a suitable method for the study of the hydrated samples. In most cases it can be alternatively adopted instead of commonly used mid-infrared (MIR) vibrational spectroscopy. This technique was applied for the first time to identify the spectral changes associated with the conformational changes in the hydrophobic region of model lipid bilayers. Trans/gauche isomerization of CH2 groups of lipid hydrocarbon chains is accompanied by characteristic changes in spectral parameters of both νas,s CH2 bands and their first overtones (2νas,s CH2). The heating of all types of analyzed liposomes results in main phase transition (Tm) accompanied by trans to gauche isomerization of CH2 groups of lipid hydrocarbon chains. The NIR-spectroscopy was able to describe in proper way (similar to MIR results) the character of Tm in studied bilayers.
- Źródło:
-
Wiadomości Chemiczne; 2023, 77, 7-8; 647--686
0043-5104
2300-0295 - Pojawia się w:
- Wiadomości Chemiczne
- Dostawca treści:
- Biblioteka Nauki
Artykuł