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Wyszukujesz frazę "badania DNA" wg kryterium: Wszystkie pola

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    Wyświetlanie 1-4 z 4
    Tytuł:
    Postglacjalna migracja jodły pospolitej (Abies alba Mill.) do Polski - analiza na podstawie polimorfizmu mitochondrialnego DNA
    Postglacial migration of silver fir (Abies alba Mill.) to Poland - analysis on the basis of mitochondrial DNA polymorphism
    Autorzy:
    Pawlaczyk, E. M.
    Kroplewska, I.
    Bobowicz, M. A.
    Powiązania:
    https://bibliotekanauki.pl/articles/991708.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    drzewa lesne
    migracje roslin
    okres polodowcowy
    Polska
    jodla pospolita
    Picea abies
    populacje roslin
    badania molekularne
    DNA mitochondrialny
    polimorfizm DNA
    silver fir (abies alba mill.)
    refugium
    postglacial migration
    mitochondrial marker
    Opis:
    Mitochondrial DNA marker was applied to 10 populations of silver fir from Poland and one from Belarusia. These populations were located within and beyond the natural range of silver fir. The marker exhibited two highly conserved alleles (first – 230 bp and second – 150 bp) based on an insertion−deletion of 80 bp in the fourth intron of the mitochondrial nad5 gene. The geographical distribution of the maternally inherited mitochondrial variation is known to support the existence of at least two refugia with two recolonizing maternal lineages remaining largely separated throughout the range. Our results provide that in all studied populations the first allele was discovered. Therefore we postulate that the silver fir migrate to Poland from the refugium in western Europe (probably from central Italy).
    Źródło:
    Sylwan; 2013, 157, 06; 458-463
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pochodzenie świerka pospolitego (Picea abies (L.) Karst.) w Nadleśnictwie Kartuzy
    Origin of Norway spruce (Picea abies (L.) Karst.) in the Kartuzy Forest District
    Autorzy:
    Lewandowski, A.
    Szydlarski, M.
    Litkowiec, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/989990.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    Nadlesnictwo Kartuzy
    drzewa lesne
    swierk pospolity
    Picea abies
    pochodzenie roslin
    badania genetyczne
    markery genetyczne
    DNA mitochondrialny
    origin
    picea abies
    mitochondrial marker
    mitotype
    Opis:
    The origin of the Norway spruce in the Kartuzy Forest District was verified using the maternally inherited mitochondrial DNA marker mt15−D02 that shows distinct geographical distribution in Europe. We analyzed 462 trees from 26 populations. The majority of trees were of Alpine origin and they were present in all populations at a frequency of 6−90% (mean 59%). About 30% of trees were of the Carpathian origin and the least abundant (11%) were trees of north−eastern origin. The trees carrying north−eastern type of the mitochondial DNA marker were found only in 13 populations, but at a very different frequency (from 5 to 88%). The possibility of natural origin of Norway spruce in the area of Kartuzy Forest District was shortly discussed.
    Źródło:
    Sylwan; 2014, 158, 07; 509-515
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zastosowanie regionu ITS1/2 rDNA i 18S rDNA do badania mykobioty gleby leśnej
    Use of ITS1/2 rDNA and 18S rDNA in studies of the forest soil mycobiota
    Autorzy:
    Behnke-Borowczyk, J.
    Kwaśna, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/989532.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    gleby lesne
    mikroorganizmy glebowe
    grzyby glebowe
    struktura zbiorowisk
    metody badan
    metody molekularne
    DNA rybosomalny
    region ITS1/2
    region 18S
    detection
    forest
    its1/2 rdna
    ns1
    ns2
    18s rdna
    microorganisms
    mycobiota
    soil
    Opis:
    The aim of the studies was to check the usefulness of ITS1/2 rDNA and 18S rDNA regions in the molecular investigation of forest soil microbiota structure. Soil studied, originated from a 1−year−old plantation and a 40−year old stand of Scots pine located in Bierzwnik and Międzychów forest districts located 200 km apart. The hypothesis assumed that both approaches lead to the discovery of abundant microbiota communities with different structures and with rare common species. The environmental DNA was extracted with a Power Soil ® DNA Isolation Kit from two soil samples in each site. The ITS1/2 rDNA was amplified with specific primers ITS1 and ewfitsrev 1, and 18S rDNA with universal primers NS1 and NS2. PCR products were cloned into pGEM−T Easy. Inserts were primarily selected in blue/white screening on a X−gal medium. Representative clones were further selected in two separate RFLP analyses with HhaI and BsuRI restriction enzymes. Representative clones purified and sequenced using the Sanger Method in the DNA Research Centre (Poznań). Each sequence was identified to the lowest taxonomic rank. Ninety to 233 clones with DNA of 5−44 taxa including 3−37 taxa of fungi were obtained from 4 samples of soil. After application of ITS1/2 rDNA and 18S rDNA, the fungal DNA was detected respectively in 89,60−100,00% and 11,77−64,8% clones and the number of fungal species detected was respectively 12−37 and 3−19. Fungi were represented by four orders: Chytridiomycota, Zygomycota, Ascomycota and Basidiomycota. Both primers also amplified also DNA of other organisms (mostly from Animalia and Protista Kingdom) represented by 0−9 taxa. If compared, the application of forest soil microbiota structure with ITS1/2 rDNA and 18S rDNA led to detect a lower abundance of fungi and a bigger abundance of other organisms. Considering the higher number of clones and taxa recognized, the region of ITS1/2 rDNA was more effective in the studies of the soil microbiota structure. The region of 18S rDNA was efficient in local detection of Chytridiomycota and Zygomycota and of rare species of fungi from Ascomycota and Basidiomycota. Despite the deficiency of NCBI database the use of the 18S rDNA region in studies on fungal community the region should be included in molecular studies of fungal diversity. It is concluded that studies on the biodiversity of soil microorganisms need the application of a few independent methods of detection and identification.
    Źródło:
    Sylwan; 2016, 160, 07; 564-572
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zmiany w strukturze genetycznej naturalnego odnowienia dębu (Quercus petraea [Matt.] Liebl.) w odniesieniu do drzew matecznych
    Changes in genetic structure of sessile oak (Quercus petraea [Matt.] Liebl.) natural regeneration in relation to maternal trees
    Autorzy:
    Nowakowska, J.A.
    Michalska, A.
    Zachara, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/990023.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    drzewa lesne
    badania genetyczne
    dab bezszypulkowy
    Quercus petraea
    odnowienia naturalne
    struktura genetyczna
    markery DNA
    drzewa potomne
    drzewa mlode
    zroznicowanie genetyczne
    drzewa mateczne
    zmiennosc genetyczna
    ssr markers
    genetic differentiation
    natural regeneration
    Opis:
    The genetic variability of sessile oak (Quercus petraea L.) mature stand and its natural progeny was investigated. Comparison between genetic structure of parental and progeny trees was based on frequencies of nuclear microsatellite (SSR) alleles occurring in three DNA loci. A slight (4%) increase of gene pool between oak mature and progeny trees was revealed by heterozygosity level estimation, maintaining 86.3% of genetic similarity between generations. Also allele richness, partition probability of basic clustering and inbreed coefficient proved the high genetic similarity between parental and progeny of investigated oak trees. The gene flow occurred within the stands as far as rare alleles were transmitted or new ones appeared in the progenies. The results highlight the necessity of such a study for silvicultural measures taken in order to proceed natural or artificial regeneration in forest tree stand.
    Źródło:
    Sylwan; 2014, 158, 02; 83-89
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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