Temat: rapid detection - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli
Autorzy:: Zhao, L.Y.
Niu, J.H.
Gao, X.L.
Liu, C.N.
Liu, S.M.
Jiang, N.
Lv, X.P.
Zheng, S.M.
Powiązania:: https://bibliotekanauki.pl/articles/2087340.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Escherichia coli
F4 fimbriae
isothermal amplification
rapid detection
clinical samples
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 1; 143-152
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA
Autorzy:: Osinska, E.
Golke, A.
Slonska, A.
Cymerys, J.
Banbura, M.W.
Dzieciatkowski, T.
Powiązania:: https://bibliotekanauki.pl/articles/30252.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: rapid detection
equine herpesvirus
real-time polymerase chain reaction
horse
herpesvirus
Gammaherpesvirinae
Rhadinovirus
veterinary virology
Opis:: Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.
Źródło:: Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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