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Wyszukujesz frazę "herpesvirus" wg kryterium: Temat


Wyświetlanie 1-12 z 12
Tytuł:
Survival rate of Suid Herpesvirus (SuHV-1, Aujeszky`s disease virus, ADV) in composted sewage sludge
Autorzy:
Paluszak, Z.
Lipowski, A.
Ligocka, A.
Powiązania:
https://bibliotekanauki.pl/articles/30422.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
survival rate
Suid herpesvirus 1
herpesvirus
composted sewage sludge
Aujeszky's disease
Aujeszky's disease virus
Opis:
Sewage sludge constitutes a source of valuable biogenic raw materials, but it is a carrier of many pathogenic microorganisms and viruses. Subjected to an effective sanitization by means of the process of composting, it is suitable to use in agriculture as fertilizers. The aim of this study was to observe the survival rate of Suid Herpesvirus under the influence of the temperature alone (water bath) as well as in sewage sludge subjected to the process of composting (pile). The samples were taken at different time intervals, and the virus titres were determined. The viruses survived considerably longer under laboratory conditions: at 30°C as long as 21 days, at 40°C - 93 hours, and at 50°C - less than an hour. In the compost pile, in spite of the lack of the thermophylic phase, the total survival time of the viruses ranged from 34 to 44.5 hours, which indicates the vast importance of other physicochemical factors, apart from the temperature, contributing to virus inactivation.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA
Autorzy:
Osinska, E.
Golke, A.
Slonska, A.
Cymerys, J.
Banbura, M.W.
Dzieciatkowski, T.
Powiązania:
https://bibliotekanauki.pl/articles/30252.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rapid detection
equine herpesvirus
real-time polymerase chain reaction
horse
herpesvirus
Gammaherpesvirinae
Rhadinovirus
veterinary virology
Opis:
Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An in vitro assessment of antiviral activity for ethanol extract of Desmodium canadense against bovine herpesvirus type 1
Autorzy:
Lelešius, R.
Girdauskaitė, P.
Karpovaitė, A.
Mickienė, R.
Drevinskas, T.
Tiso, N.
Ragažinskienė, O.
Kubilienė, L.
Maruška, A.
Šalomskas, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087312.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bovine herpesvirus 1
plant extracts
Desmodium canadense
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 177-184
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reactive oxygen metabolitesin alpha-herpesvirus-seropositive Mediterranean buffaloes (Bubalus bubalis): a preliminary study
Autorzy:
Tafuri, S.
Marullo, A.
Ciani, F.
Della Morte, R.
Montagnaro, S.
Fiorito, F.
De Martino, L.
Powiązania:
https://bibliotekanauki.pl/articles/2087691.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Bubalus bubalis
Bubaline herpesvirus 1 (BuHV-1)
Bovine herpesvirus 1 (BoHV-1)
reactive oxygen metabolites (ROM)
oxidative stress index (Osi)
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 639-642
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of administering activated lymphocytes originated from the dam on the immune cell reaction in Holstein calves
Autorzy:
Ohtsuka, H.
Yamaguchi, T.
Maeda, Y.
Tomioka, M.
Tajima, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087343.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bovine herpesvirus
calves
dam
immune cells
lymphokine activated killer
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 109-117
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of an indirect ELISA based on glycoprotein B gene for detecting of Feline herpesvirus type 1
Autorzy:
Tan, Y.
Dong, G.
Niu, J.
Guo, Y.
Yi, S.
Sun, M.
Wang, K.
Hu, G.
Powiązania:
https://bibliotekanauki.pl/articles/2087468.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
feline herpesvirus type 1
gB protein
indirect ELISA
prokaryotic expression
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 631-633
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection
Autorzy:
Golke, A.
Cymerys, J.
Slonska, A.
Dzieciatkowski, T.
Chmielewska, A.
Tucholska, A.
Banbura, M.W.
Powiązania:
https://bibliotekanauki.pl/articles/30665.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
xCELLigence system
real-time analysis
label-free analysis
neuronal cell response
dermal cell response
cell response
equine herpesvirus 1
animal infection
herpesvirus
neuron
neuronal cell
dermal cell
Opis:
Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Equine herpesvirus type 1 affects mitochondrial network morphology and reactive oxygen species generation in equine dermal cell line
Autorzy:
Bartak, M.
Chodkowski, M.
Słońska, A.
Bańbura, M.W.
Cymerys, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087318.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
equine herpesvirus type 1
equine dermal cell line
mitochondria
reactive oxygen species
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 309-312
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture
Autorzy:
Cymerys, J.
Dzieciatkowski, T.
Slonska, A.
Bierla, J.
Tucholska, A.
Chmielewska, A.
Golke, A.
Banbura, M.W.
Powiązania:
https://bibliotekanauki.pl/articles/31974.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
replication
animal disease
horse
equine herpesvirus 1
respiratory tract infection
neurological disorder
abortion
neuron
Opis:
Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serological evidence of lack of contact with caprine herpesvirus type 1 and bluetongue virus in goat population in Poland
Autorzy:
Czopowicz, M.
Kaba, J.
Szalus-Jordanow, O.
Nowicki, M.
Witkowski, L.
Frymus, T.
Powiązania:
https://bibliotekanauki.pl/articles/30762.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
serological evidence
caprine herpesvirus type 1
bluetongue virus
goat
population
Polska
seroprevalence
small ruminant
animal infection
Opis:
Investigation into herd-level seroprevalence of caprine herpesvirus type 1 (CpHV-1) and bluetongue virus (BTV) was conducted in 2007 in Poland. It involved the entire population of goats covered by a milk recording program in 2007, which included 49 goat herds. The number of goats examined in each herd was determined statistically in order to detect the presence of at least one seropositive animal in a herd with a 95% probability and simple random method of sampling was applied. No antibodies to CpHV-1 or BTV were detected. Further calculations were carried out to determine the herd-level true seroprevalence, taking into account sensitivity and specificity of the test as well as several other factors. It can be concluded that till the middle of 2007 population of Polish goats covered by the milk recording program remained negative with respect to CpHV-1 and BTV.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of BHV-1 (bovine herpesvirus type 1) among non-vaccinated dairy cattle herds with respiratory disorders
Autorzy:
Rypula, K.
Ploneczka-Janeczko, K.
Kita, J.
Kumala, A.
Zmudzinski, J.F.
Powiązania:
https://bibliotekanauki.pl/articles/30913.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
seroprevalence
bovine herpesvirus type 1
non-vaccinated animal
dairy cattle
herd
respiratory disorder
ELISA test
seroconversion
Opis:
The objective of this study was to estimate a herd-level seroprevalence of bovine herpesvirus type 1 (BHV-1) in herds with clinical symptoms of the respiratory tract. Eighty-three herds with suspected BHV-1 infection were selected and divided into two categories with respect to their size: small (n=27) and large herds (n=56). Samples were collected from calves, heifers and cows older than 24 months. Seroprevalence was determined using the gB ELISA test. The herd level seroprevalence was estimated as 53% (44/83) in the tested herds, 11.1% (3/27) in the small herds and 73.2% (41/56) in the large herds. Our study suggests that the current biosecurity measures still warrant improvement.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR
Autorzy:
Krzyzankova, M.
Krasna, M.
Prodelalova, J.
Vasickova, P.
Powiązania:
https://bibliotekanauki.pl/articles/16647567.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
African swine fever virus
bovine herpesvirus-1
palladium compounds
platinum compounds
molecular methods
viability PCR
Opis:
Cultivation-based assays represent the gold standard for the assessment of virus infectivity; however, they are time-consuming and not suitable for every virus type. Pre-treatment with platinum (Pt) compounds followed by real-time PCR has been shown to discriminate between infectious and non-infectious RNA viruses. This study examined the effect of Pt and palladium (Pd) compounds on enveloped DNA viruses, paying attention to two significant pathogens of livestock – bovine herpesvirus-1 (BoHV-1) and African swine fever virus (ASFV). Native or heat-treated BoHV-1 suspension was incubated with the spectrum of Pt/Pd compounds. Bis(benzonitrile)palladium(II) dichloride (BB-PdCl 2) and dichloro(1,5-cyclooctadiene) palladium(II) (PdCl 2-COD) produced the highest differences found between native and heat- -treated viruses. Optimized pre-treatment conditions (1 mM of Pd compound, 15 min, 4°C) were applied on both virus genera and the heat inactivation profiles were assessed. A significant decrease in the detected quantity of BoHV-1 DNA and ASFV DNA after heat-treatment (60°C and 95°C) and consequent incubation with Pd compounds was observed. BB-PdCl 2 and PdCl 2-COD could help to distinguish between infectious and non-infectious enveloped DNA viruses such as BoHV-1 or ASFV.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 2; 211-221
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-12 z 12

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