Temat: electrophoresis - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Peripartum changes in serum activities of three major alkaline phosphatase isoenzymes in Holstein dairy cows
Autorzy:: Chiba, A.
Onomi, R.
Hatate, K.
Moriyama, T.
Goto, A.
Yamagishi, N.
Powiązania:: https://bibliotekanauki.pl/articles/2087301.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: agarose gel electrophoresis (AGE)
alkaline phosphatase isoenzyme
periparturient cow
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 3; 457-459
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation
Autorzy:: Chiba, A.
Hatate, K.
Onomi, R.
Moriyama, T.
Goto, A.
Yamagishi, N.
Powiązania:: https://bibliotekanauki.pl/articles/2087309.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: agarose gel electrophoresis (AGE)
alkaline phosphatase isoenzyme
lactating cow
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 2; 317-319
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Isolation and characterization of zinc-binding proteins of canine seminal plasma
Autorzy:: Mogielnicka-Brzozowska, M.
Fraser, L.
Czarzasta, J.
Kordan, W.
Powiązania:: https://bibliotekanauki.pl/articles/32311.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: isolation
zinc-binding protein
seminal plasma
dog
electrophoresis
reproductive process
Opis:: Zinc-binding proteins from seminal plasma (ZnBPs) originate in the secretions of different accessory sex glands and are implicated in key events associated with sperm-egg fertilization processes. This study describes the isolation and characterization of the ZnBPs of canine seminal plasma. Ejaculates were collected from three crossbred dogs for a 2-week period. The ZnBPs as well as non zinc-binding proteins (nZnBPs) were isolated by zinc-dependent affinity chromatography. The isolated fractions were subjected to native gel electrophoresis (one-dimensional polyacryamide gel electrophoresis, PAGE) and sodium dodecyl sulphate polyacryamide gel electrophoresis (SDS-PAGE), using denaturing and reducing conditions. Zinc-elution profile using affinity chromatography displayed two protein fractions represented by the nZnBPs and ZnBPs, respectively. Using native gel electrophoresis, it was found that both the nZnBPs and ZnBPs occurred in their native state as aggregates, ranging from 140 to 669 kDa. The nZnBPs were disaggregated into 8 protein bands, with molecular weights ranging from 10.7 to 79.7 kDa, following SDS-PAGE analysis. By contrast, SDS-PAGE analysis of the ZnBPs revealed 13 protein bands, with molecular weights ranging from 11.6 to 152.3 kDa. Densitometric analysis showed that 46-48% of nZnBPs could be accounted by protein fractions with molecular weights of 10.7 and 14.2 kDa. Also, 2 protein fractions with molecular weights of 11.6 and 14.3 kDa, were predominant in ZnBPs, accounting for approximately 28-30% of the total proteins. These results demonstrate the zinc-binding capacity of proteins secreted by the canine prostate. The findings of this study indicate that ZnBPs of canine seminal plasma comprise several protein fractions, which might be implicated in the reproductive processes in the dog.
Źródło:: Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Determination of plasma bone-specific alkaline phosphatase isoenzyme activity in Holstein calves using a commercial agarose gel electrophoresis kit
Autorzy:: Onomi, R.
Hatate, K.
Yamagishi, N.
Powiązania:: https://bibliotekanauki.pl/articles/2087395.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: agarose gel electrophoresis (AGE)
bone-specific alkaline phosphatase isoenzyme
calf
Źródło:: Polish Journal of Veterinary Sciences; 2019, 4; 789-792
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Heterogeneity of methicillin-sensitive Staphylococcus pseudintermedius strains isolated from diseased dogs
Autorzy:: Garbacz, K.
Piechowicz, L.
Zarnowska, S.
Haras, K.
Dabrowska-Szponar, M.
Powiązania:: https://bibliotekanauki.pl/articles/32033.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: animal disease
dog
heterogeneity
methicillin
Staphylococcus pseudintermedius
isolation
diseased animal
pulsed-field gel electrophoresis
Opis:: Thirty nine canine S. pseudintermedius strains were examined for antibiotic susceptibility and genetic polymorphisms. All strains were methicillin-sensitive S. pseudintermedius (MSSP). Resistance to penicillin was most prevalent (66.6%), followed by resistance to neomycin (56.4%), erythromycin (53.8%), clindamycin (48.7%), chloramphenicol (48.7%), and tetracycline (46.2%). Pulsed-field electrophoresis (PFGE) showed a high genetic polymorphism in the investigated strains.
Źródło:: Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Molecular forms of selected antioxidant enzymes in dog semen – electrophoretical identification
Autorzy:: Koziorowska-Gilun, M.
Strzezek, R.
Powiązania:: https://bibliotekanauki.pl/articles/32130.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: molecular form
antioxidative enzyme
dog
semen
electrophoresis
identification
spermatozoon
enzyme
superoxide dismutase
catalase
glutathione peroxidase
reactive oxygen species
Opis:: The aim of the study was the electrophoretical identification of molecular forms of selected antioxidant enzymes in dog semen. Ejaculates to be studied were chosen from five dogs, aged from two to eight years. Polyacrylamide gel electrophoresis was carried out under non-denaturing conditions and then gels were stained for the activity of the following enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Sperm homogenates and all fractions (pre-spermatic, spermatic and post-spermatic) of dog ejaculate demonstrated one protein band with SOD activity characterized by low electrophoretic mobility. Based on the confirmed sensitivity to H₂O₂, it can be assumed that the detected SOD is an enzyme containing ions of Zn²⁺ and Cu²⁺ (Cu,Zn SOD). In sperm homogenates one protein band with GPx activity was characterized by high electrophoretic mobility, whereas in the spermatic and post-spermatic fractions of dog ejaculate three protein bands with different (low, medium and high) electrophoretic mobility were identified. CAT molecular forms were not found in either sperm homogenates or in the analyzed fractions of ejaculate.
Źródło:: Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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