Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "Zhang, S.-Y." wg kryterium: Autor

  Lista filtrów
Wyrównaj
    Wyświetlanie 1-11 z 11
    Tytuł:
    Expression analysis of multifunctional RNA-binding protein hnRNP K during development of mammalian testis
    Autorzy:
    Xu, H.
    Zhang, P.
    Li, R.
    Wu, W.
    Wang, S.
    Xu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087724.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    hnRNP K
    testis development
    spermatogenesis
    pig
    rat
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 343-352
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymorphic analysis of peptide binding domain of major histocompatibility complex class I in domestic ducks
    Autorzy:
    Yu, S.
    Wu, J.
    Bai, J.
    Ding, Y.
    Qiu, W.
    Zhang, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087551.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    domestic duck
    highly variably sites
    MHC class I
    peptide binding domain
    phylogenetic tree
    polymorphism
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 415-422
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Modulation of virulence genes by the two-component system PhoP - PhoQ in avian pathogenic Escherichia coli
    Autorzy:
    Tu, J.
    Huang, B.
    Zhang, Y.
    Xue, T.
    Li, S.
    Qi, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/30418.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Avian pathogenic Escherichia coli (APEC) infections are a very important problem in the poultry industry. PhoP-PhoQ is a two-component system that regulates virulence genes in APEC. In this study, we constructed strains that lacked the PhoP or PhoQ genes to assess regulation of APEC pathogenicity by the PhoP-PhoQ two-component system. The PhoP mutant strain AE18, PhoQ mutant strain AE19, and PhoP/PhoQ mutant strain AE20 were constructed by the Red homologous recombination method. Swim plates were used to evaluate the motility of the APEC strains, viable bacteria counting was used to assess adhesion and invasion of chick embryo fibroblasts, and Real-Time PCR was used to measure mRNA expression of virulence genes. We first confirmed that AE18, AE19, and AE20 were successfully constructed from the wild-type AE17 strain. AE18, AE19, and AE20 showed significant decreases in motility of 70.97%, 83.87%, and 37.1%, respectively, in comparison with AE17. Moreover, in comparison with AE17, AE18, AE19, and AE20 showed significant decreases of 63.11%, 65.42%, and 30.26%, respectively, in CEF cell adhesion, and significant decreases of 59.83%, 57.82%, and 37.90%, respectively, in CEF cell invasion. In comparison with AE17, transcript levels of sodA, polA, and iss were significantly decreased in AE18, while transcript levels of fimC and iss were significantly decreased in AE19. Our results demonstrate that deletion of PhoP or PhoQ inhibits invasion and adhesion of APEC to CEF cells and significantly reduces APEC virulence by regulating transcription of virulence genes.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biological characteristics of a new antibacterial peptide and its antibacterial mechanisms against Gram-negative bacteria
    Autorzy:
    Pei, Z.
    Ying, X.
    Tang, Y.
    Liu, L.
    Zhang, H.
    Liu, S.
    Zhang, D.
    Wang, K.
    Kong, L.
    Gao, Y.
    Ma, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087656.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    antimicrobial peptide
    biological characteristics
    antibacterial mechanism
    Gram-negative bacteria
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 533-542
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Establishment of 5-Fluorouracil-resistant canine mammary tumor cell line
    Autorzy:
    Zhou, B.
    Zhang, D.
    Pei, S.M.
    Zhang, K.
    Du, H.C.
    Jin, Y.P.
    Lin, D.G.
    Powiązania:
    https://bibliotekanauki.pl/articles/30283.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Canine mammary tumors are the most common neoplasms in intact female dogs. The surgery cannot always solve the problem, chemotherapy are recommend to these patients. However, chemotherapy could always fail because of multidrug resistance (MDR). Through stepwise increasing 5-Fluorouracil (5-FU) concentration in the culture medium, a 5-FU-resistant canine mammary tumor cell line CMT7364/5-FU was established to disclose the molecular mechanism of the drug resistance. Cell morphology, cell sensitivity to drugs, growth curves, expression of proteins, and chemo-sensitivity in vivo were compared between the parental cell line and resistant cell line. As compared it to its parental cell line (CMT7364), CMT7364/5-FU showed different morphology, cross-resistant to other chemo-drugs and a prolonged population doubling time (PDT). The drug efflux pump proteins (ABCB1 and ABCG2) in CMT7364/5-FU were up-regulated. In vivo, the similar result revealed that CMT7364/5-FU cell line was more resistant to 5-FU. In conclusion, a 5-FU-resistant canine mammary tumor cell line (CMT7364/5-FU) was successfully established, it can serve as a good model for researching the mechanism of MDR and screening effective agents to reverse drug resistance.
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 20, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    GP5 protein - based ELISA for the detection of PRRSV antibodies
    Autorzy:
    Wang, Y.
    Guo, J.
    Qiuao, S.
    Li, Q.
    Yang, J.
    Jin, Q.
    Zhang, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/30629.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Porcine reproductive and respiratory syndrome virus (PRRSV) is an important swine pathogen, causing huge economic losses each year worldwide. Immunization with vaccines containing the glycoprotein 5 (GP5) of PRRSV is the main measure to induce neutralizing antibodies and control the disease. Here, we developed a GP5 protein-based ELISA for detecting antibodies against PRRSV. The overall yield of purified GP5 in E. coli flask culture was more than 45 mg/L cell culture. Western blot and IFA indicated that the GP5 protein was highly immunogenic. After optimization and validation with IDEXX PRRS using 566 clinical sera, the DSN, DSP, and accuracy of GP5-ELISA were 81.39%, 75.96%, and 80.39%, respectively. Besides, GP5-ELISA is highly specific, showing no cross-reactions with sera against other important swine pathogens. Hence, GP5 is a good diagnostic antigen and the GP5 protein-based ELISA has the potential to be used in the field
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus
    Autorzy:
    Lin, A.
    Hu, X.
    Cui, S.
    Yang, T.
    Zhang, Z.
    Li, P.
    Guo, M.
    Lu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647453.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Getah virus
    real-time PCR
    TaqMan
    detection
    Opis:
    To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 21-28
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Selection of reference genes for quantitative real-time RT-PCR on gene expression in Golden Pompano (Trachinotus ovatus)
    Autorzy:
    Chen, X.J.
    Sun, Y.
    Zhang, X.Q.
    Huang, S.
    Cao, Z.J.
    Qin, Q.W.
    Hu, W.T.
    Zhou, Y.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087908.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 3; 583-594
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    iTRAQ-based quantitative proteomics analysis reveals inhibitory mechanismsof the antimicrobial peptide MDAP-2 against Salmonella gallinarum
    Autorzy:
    Zhang, Y.
    Yu, S.
    Ying, X.
    Jia, B.
    Liu, L.
    Liu, J.
    Kong, L.
    Pei, Z.
    Ma, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087299.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Salmonella gallinarum
    AMP
    iTRAQ
    inhibitory mechanism
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 3; 405-414
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular characterization and expression patterns of ghrelin in the reindeer (Rangifer tarandus)
    Autorzy:
    Zhang, M.
    Xu, X.
    Zhu, X.
    Jin, X.
    Bao, H.
    Dugeer, S.
    Du, C.
    Cao, G.
    Yang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087780.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ghrelin
    reindeer (Rangifer tarandus)
    cDNA cloning
    structural characterization
    tissue expression
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 55-64
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
    Autorzy:
    Jin, Q.Y.
    Feng, L.L.
    Wang, Y.B.
    Li, P.
    Yang, J.F.
    Teng, M.
    Chai, S.J.
    Xing, G.X.
    Zhang, G.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539073.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    porcine circovirus type 2 (PCV2)
    screening of MAbs
    paper test
    Opis:
    A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

      Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies