Autor: Yang, F - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Detection of self-biting behavior of mink by loop-mediated isothermal amplification (LAMP) and sequence-characterized amplified regions (SCAR)
Autorzy:: Liu, Z.Y.
Song, S.S.
Huo, Z.S.
Song, X.C.
Cong, B.
Yang, F.H.
Powiązania:: https://bibliotekanauki.pl/articles/2087715.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: mink
self-biting
loop-mediated isothermal amplification
sequence-characterized amplified regions
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 2; 371-376
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: The study on bactericidal effect and ultrastructural alterations of chlorocresol nanoemulsion disinfectant against Staphylococcus aureus
Autorzy:: Zhang, Y.F.
Sun, Y.W.
Liu, X.H.
An, Z.X.
Yang, X.F.
Powiązania:: https://bibliotekanauki.pl/articles/16647452.pdf
Data publikacji:: 2023
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: chlorocresol
nanoemulsion
disinfectant
Staphylococcus aureus
bactericidal effect
ultrastructure
Opis:: Chlorocresol nanoemulsion disinfectant (CND) is an environmental disinfectant prepared with nanoemulsion as its drug carrier. This study aimed to investigate the bactericidal effect of CND on Staphylococcus aureus ( S. aureus) and its effect on bacterial ultrastructure. The neutralizing effect of CND against S. aureus was first screened by suspension quantitative evaluation experiment procedure of neutralizer. Disinfection performance was evaluated by the determination of Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), quantitative bactericidal experiment, and comparative experiment of disinfection performance between 0.1% CND and 0.1% chlorocresol aqueous solution. Meanwhile, the effect of CND on the ultrastructure of S. aureus was investigated with scanning electron microscope (SEM) and transmission electron microscope (TEM) to preliminarily explore the bactericidal mechanism. The results showed that 3% Tween-80 in PBS could be screened as the neutralizer of CND against S. aureus. MIC and MBC were 100 μg/mL and 200 μg/mL, respectively. The bactericidal rates were all 100% when 0.06% and 0.08% disinfectant acted for 15 and 5 min, respectively. Furthermore, compared with 0.1% chlorocresol aqueous solution, the bactericidal effect of 0.1% CND was significantly enhanced (p<0.01). After treatment with CND for 10 min, SEM observation showed that the morphology of S. aureus cells were changed and the integrity destroyed. TEM observation showed that the cell shape changed, and the structures of the cell wall, cell membrane and cytoplasm were damaged in varying degrees. CND showed the strong bactericidal effect on S. aureus and could cause ultrastructure alterations of S. aureus.
Źródło:: Polish Journal of Veterinary Sciences; 2023, 26, 1; 13-20
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Pharmacokinetics of diclofenac sodium injection in swine
Autorzy:: Yang, H.F.
Li, Y.J.
Li, Y.Y.
Huang, C.
Huang, L.X.
Bu, S.J.
Powiązania:: https://bibliotekanauki.pl/articles/2087550.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: diclofenac sodium injection
pharmacokinetics
bioavailability
swine
HPLC
Źródło:: Polish Journal of Veterinary Sciences; 2019, 2; 423-426
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:: Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:: https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:: A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Caspase-1 inhibitor reduced the lung injury in a mouse model of pleuropneumonia caused by Actinobacillus pleuropneumoniae
Autorzy:: Zhang, Y.
Yang, T.
Huang, F.
Powiązania:: https://bibliotekanauki.pl/articles/2087250.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: porcine contagious pleuropneumonia
mice
caspase-1 inhibitors
caspase-1dependent pyroptosis
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 4; 605-610
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:: Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:: https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:: Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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