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Wyszukujesz frazę "Yang, D. D." wg kryterium: Autor


Wyświetlanie 1-5 z 5
Tytuł:
Molecular characterization and expression analysis of the NLR family CARD containing five transcripts in the pig
Autorzy:
Yang, Q.Y.
Chen, T.
Chen, Y.B.
Lan, D.L.
Powiązania:
https://bibliotekanauki.pl/articles/32088.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The NOD-like receptor (NLR) family caspase recruitment domain-containing 5 (NLRC5) is one of the newly discovered and largest NLR family members. The NLRC5 has recently received extensive attention because of its important role in regulating innate and adaptive immune responses. The NLRC5 in many vertebrates, such as humans, mice, cattle, and horses, has already been proven and studied. However, the NLRC5 gene characteristics of pigs remain unclear. Thus, we completely cloned the NLRC5 cDNA sequence of the pig using the rapid amplification of cDNA ends(RACE) technology. A characteristic and tissue expression analysis was also conducted on the pig sequence. The sequence analysis showed that the complete cDNA sequence of the NLRC5 of the pig is 6638 bp, and the open reading frame is 5538 bp which encoded 1846 amino acids. The protein prediction analysis indicates that the overall performance of the NLRC5 protein of the pig is hydrophilic and possesses a typical nucleotide binding and oligomerization domain(NBD) and 20 leucine-rich repeats(LRRs). The homology analysis result indicates that the NLRC5 transcript in pigs is highly homologous to cattle, sheep, macaques, and humans, and accounts for around 80%. The genetic evolutionary tree analysis shows that the NLRC5 transcript in pigs has the closest evolutionary relationship with cattle and sheep. Further tissue expression analysis shows that immune organ systems (e.g., lymph node and spleen) and mucosa organs (e.g., intestinal lymph node, stomach, and lungs) possess high expressions with NLRC5 mRNA. The result of this study indicates that the NLRC5 transcript in pigs is relatively conservative among mammals and may play a vital role in immune reaction, which provides a basis for further studies on the NLRC5 function in the pig immune system and the role in comparative immunity.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SYBR Green-based real-time polymerase chain reaction assay for detection of porcine parvovirus 6 in pigs
Autorzy:
Sun, P.
Bai, C.X.
Zhang, D.
Wang, J.
Yang, K.K.
Cheng, B.Z.
Li, Y.D.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087328.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
porcine parvovirus 6
real-time polymerase chain reaction
SYBR Green
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 197-202
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:
Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
Autorzy:
Xu, T.
Huang, X.
Li, Y.
Liu, Q.
Liu, X.
Han, K.
Liu, Y.
Yang, J.
Zhao, D.
Bi, K.
Sun, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087719.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goose-derived
duck-derived
chicken-derived
Tembusu virus
pathogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-5 z 5

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