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    Wyświetlanie 1-10 z 10
    Tytuł:
    Expression analysis of multifunctional RNA-binding protein hnRNP K during development of mammalian testis
    Autorzy:
    Xu, H.
    Zhang, P.
    Li, R.
    Wu, W.
    Wang, S.
    Xu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087724.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    hnRNP K
    testis development
    spermatogenesis
    pig
    rat
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 343-352
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dynamic expression profile of DNA methyltransferases in rat testis development
    Autorzy:
    Xu, H.X.
    Qin, J.Z.
    Zhang, K.Y.
    Zeng, W.X.
    Powiązania:
    https://bibliotekanauki.pl/articles/31635.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    DNA methyltransferases (Dnmts) are unique and perform specific functions during male germ cell development. To further characterize the significance of Dnmts in the events leading to production of spermatozoa, we investigated whether the expression patterns in Dnmt1, Dnmt3a, Dnmt3b and Dnmt3l were apparent in rat testes at different time points during development. The qRT-PCR results showed that expression levels of Dnmt3a and Dnmt3l were abundant before birth and were present at the highest levels in testes tissue at 18.5 days postcoitus (dpc), and gradually decreased from day 0 postpartum (dpp) to 90 dpp. Expression of Dnmt1 and Dnmt3b reached a peak after birth (P <0.01), and then gradually reduced until adulthood. Western blotting and immunolocalization analysis of Dnmt3a and Dnmt3b further confirmed the differential expression and localization of the two proteins during rat testis development. The dynamic expression profile of Dnmts implies specific and potentially nonredundant roles for each of these enzymes in the developing rat testis.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibiofilm activity against Staphylococcus aureus and content analysis of Taraxacum Officinale phenolic extract
    Autorzy:
    Xu, P.
    Xu, X.B.
    Khan, A.
    Fotina, T.
    Wang, S.H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087162.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Taraxacum Officinale
    phenolic
    antibacterial mechanism
    HPLC
    Staphylococcus aureus
    Źródło:
    Polish Journal of Veterinary Sciences; 2021, 24, 2; 243-251
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Review and future perspectives on the integration characteristics for equine lentivirus in the host genome
    Autorzy:
    Yu, Y.-Y.
    Xu, M.-S.
    Liang, H.
    Wang, H.-Y.
    Yu, C.-Q.
    Liu, Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647532.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    lentivirus
    equine infectious anemia virus
    integration
    long interspersed elements
    Opis:
    Despite over 40 years of research on the human immunodeficiency virus type 1 (HIV-1) vaccine, we still lack a considerable progress. Equine infectious anemia virus (EIAV) is a lentivirus in the Retroviridae family, akin to HIV-1 in genome structure and antigenicity. EIA is an important infectious disease in equids, characterized by anemia, persistent infection, and repeated fevers. The EIAV attenuated vaccine in China is the only lentiviral vaccine used on a large scale. Elucidating the mechanism of waning and induction of protective immunity from this attenuated vaccine strain will provide a critical theoretical basis and reference point for vaccine research, particularly in the development of lentivirus vaccines, with far-reaching scientific value and social significance. In this paper, we summarize the information related to EIAV integration site selection, particularly for the Chinese EIAV attenuated vaccine strains on the equine genome. This may improve our mechanistic understanding of EIAV virulence reduction at the host genome level. The obtained data may help elucidate the biological characteristics of EIAV, particularly the Chinese attenuated EIAV vaccine strain, and provide valuable information regarding retroviral infections, particularly lentiviral infection and associated therapeutic vectors.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 163-172
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular cloning, expression, purification and osteoblasts proliferation activity of sika deer thymosin beta10
    Autorzy:
    Zhao, D.
    Zhao, Y.
    Zhang, H.
    Xu, Z.
    Liu, M.
    Wang, J.
    Bai, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087874.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    affinity chromatography
    cell proliferation
    prokaryotic expression
    sika deer
    thymosin 10
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 4; 751-758
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig
    Autorzy:
    Hua, Z.
    Xu, G.
    Liu, X.
    Bi, Y.
    Xiao, H.
    Hua, W.
    Li, L.
    Zhang, L.
    Ren, H.
    Zheng, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/31224.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for both agricultural and biomedical research. However, its efficiency is crucially dependent on the source of nuclear donor cells. In this study, we compared the cloning efficiency by using three lines of donor cells that are derived from fetal, newborn and adult fibroblasts of Chinese indigenous Meishan pig. We showed that cleavage rate and blastocyst formation rate of the reconstructed embryos were not significantly different between the fetal (80.7% and 15.6%) and newborn ear skin (77.5% and 12.3%) fibroblast groups (p>0.05), but in both groups these indices were significantly higher than that found in the adult ear skin (70.5% and 8.8%; p<0.05). Reconstructed embryos derived from fetal, newborn, and adult ear skin fibroblasts were transferred to four surrogates, respectively. For the fetal, newborn, and adult ear skin fibroblasts, the number of pregnancies were two (50.0%), two (50.0%), and one (25.0%), respectively, and the number of deliveries were two (50.0%), one (25.0%), and zero (0.0%), respectively. Seven and two cloned piglets were obtained from the fetal and newborn ear skin fibroblasts respectively, while no piglets were obtained from the adult ear skin fibroblasts. Two cloned piglets from the newborn ear skin fibroblasts died shortly after birth because of neonatal asphyxia caused by dystocia. The birth weights of the piglets derived from the fetal and newborn ear skin fibroblasts were 1230.5 and 1310.0g, respectively, which were statistically insignificant (p>0.05), but both were significantly higher than that of the control groups (p<0.05). Microsatellite analyses demonstrated that the genotypes of all cloned piglets were identical to their donor cells. Therefore, cloned pigs were successfully produced using two sources of donor cells isolated from the fetal and newborn ear skin fibroblasts of Meishan piglet, and indicating a better cloning efficiency than that obtained from adult fibroblasts. We concluded that the nuclear donor cell lines have significant impact on the developmental competence of cloned embryos as well as on the cloning efficiency of Meishan pig.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Magnolol inhibits Streptococcus suis-induced inflammation and ROS formation via TLR2/MAPK/NF-κB signaling in RAW264.7 cells
    Autorzy:
    Wang, J.
    Sun, Y.
    Zhang, L.
    Xu, W.
    You, J.
    Lu, H.
    Song, Y.
    Wei, J.
    Li, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087779.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    magnolol
    Streptococcus suis
    Toll-like receptor2
    mitogen-activated protein kinase
    nuclear factor Kappa B
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 111-118
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular characterization and expression patterns of ghrelin in the reindeer (Rangifer tarandus)
    Autorzy:
    Zhang, M.
    Xu, X.
    Zhu, X.
    Jin, X.
    Bao, H.
    Dugeer, S.
    Du, C.
    Cao, G.
    Yang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087780.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ghrelin
    reindeer (Rangifer tarandus)
    cDNA cloning
    structural characterization
    tissue expression
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 55-64
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Induction of immune responses in sheep by vaccination with liposome-entrapped DNA complexes encoding Toxoplasma gondii MIC3 gene
    Autorzy:
    Hiszczynska-Sawicka, E.
    Li, H.
    Xu, J.B.
    Akhtar, M.
    Holec-Gasior, L.
    Kur, J.
    Bickerstaffe, R.
    Stankiewicz, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/30828.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    immune response
    sheep
    vaccination
    Toxoplasma gondii
    parasite
    protozoan parasite
    pregnancy
    MIC3 gene
    MIC3 antigen
    Opis:
    Toxoplasma gondii is a parasite that has been extensively studied due to its medical and veterinary importance in terminating pregnancies. Consequently, a satisfactory vaccine is required to control its adverse effects on pregnant animals. The microneme protein, MIC3, is a major adhesion protein that binds to the surface of host cells and parasites, and is therefore a potential vaccine against T. gondii. The viability of MIC3 as a vaccine is investigated in this study. Sheep were injected twice, intramuscularly, with plasmids containing DNA encoding for the mature form of MIC3 protein formulated into liposomes. Control sheep were injected with an empty vector or received no injections. The injection of sheep with DNA plasmids encoding for MIC3 elicited an immune response after the first and second injections as indicated by antibody responses and the production of IFN-γ. The immune response, as measured by the IgG2 and IgG1 serum levels, was boosted after the injection of the MIC3 DNA vaccine together with high anti-MIC3 antibodies. The results demonstrate that the intramuscular injection of sheep with a plasmid containing DNA coding for MIC3 protein induces a significant and effective immune response against T. gondii.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bacterial communities in PM2.5 and PM10 in broiler houses at different broiler growth stages in spring
    Autorzy:
    Zhang, J.
    Li, Y.
    Xu, E.
    Jiang, L.
    Tang, J.
    Li, M.
    Zhao, X.
    Chen, G.
    Zhu, H.
    Yu, X.
    Zhang, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087465.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bacterial communities
    broilers
    high-throughput sequencing
    particulate matter
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 3; 495-504
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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