Autor: Wu, J.W. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Effects of porcine epidemic diarrhea virus infection on tight junction protein gene expression and morphology of the intestinal mucosa in pigs
Autorzy:: Zong, Q.F.
Huang, Y.J.
Wu, L.S.
Wu, Z.C.
Wu, S.L.
Bao, W.B.
Powiązania:: https://bibliotekanauki.pl/articles/2087524.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: pig
tight junction protein
PEDV
diarrhea
Źródło:: Polish Journal of Veterinary Sciences; 2019, 2; 345-353
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Polymorphic analysis of peptide binding domain of major histocompatibility complex class I in domestic ducks
Autorzy:: Yu, S.
Wu, J.
Bai, J.
Ding, Y.
Qiu, W.
Zhang, L.
Powiązania:: https://bibliotekanauki.pl/articles/2087551.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: domestic duck
highly variably sites
MHC class I
peptide binding domain
phylogenetic tree
polymorphism
Źródło:: Polish Journal of Veterinary Sciences; 2019, 2; 415-422
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Indirect enzyme-linked immunosorbent assay method based on Streptococcus agalactiae rSip-Pgk-FbsA fusion protein for detection of bovine mastitis
Autorzy:: Bu, R.-E.
Wang, I.-L.
Wu, J.-H.
Xilin, G.W.
Chen, J.-L.
Wang, H.
Powiązania:: https://bibliotekanauki.pl/articles/30156.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: Objective: The aim of this study was to establish a rapid and accurate method for the detection of the Streptococcus agalactiae antibody (SA-Ab) to determine the presence of the bovine mastitis (BM)-causative pathogen. Methods: The multi-subunit fusion protein rSip-Pgk-FbsA was prokaryotically expressed and purified. The triple activities of the membrane surface-associated proteins Sip, phosphoglycerate kinase (Pgk), and fibronectin (FbsA) were used as the diagnostic antigens to establish an indirect enzyme-linked immunosorbent assay (ELISA) method for the detection of SA-Ab in BM. Results: The optimal antigen coating concentration was 2 μg/mL, the optimal serum dilution was 1:160, and the optimal dilution of the enzyme-labeled secondary antibody was 1:6000. The sensitivity, specificity, and repeatability tests showed that the method established in this study had no cross-reaction with antibodies to Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis in the sera. The results of the sensitivity test showed that a positive result could be obtained even if the serum dilution reached 1:12,800, indicating the high sensitivity and good repeatability of the method. The positive coincidence rate of this method was 98.6%, which is higher than that of previous tests established with the Sip or Pgk mono-antigen fusion protein, respectively, demonstrating the relatively higher sensitivity of this newly established method. The detection rate for 389 clinical samples was 46.53%. Conclusions: The indirect ELISA method established in this study could provide a more accurate and reliable serological method for the rapid detection of S. agalactiae in cases of BM.
Źródło:: Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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