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Wyświetlanie 1-63 z 63
Tytuł:
White and red blood cells picture in rabbits experimentally infected with RHD virus
Autorzy:
Niedzwiedzka-Rystwej, P.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/30544.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Four strains of RHDV assigned as haemagglutinating (Vt97 and Hartmannsdorf) and non-haemagglutinating (Pv97 and 9905) antigenic variants were examined for dynamic changes in the values of white and red blood cells indexes. The study showed differences among strains examined that were not depending on haemagglutination property.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytometric analysis of lymphocytes T and B in rabbits infected with non-haemagglutinogenic strains of RHD virus [rabbit heamorrhagic disease] - Rainham, Frankfurt and Asturias
Autorzy:
Niedzwiedzka-Rystwej, P
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/31408.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
T lymphocyte
B lymphocyte
cytometric analysis
rabbit
rabbit hemorrhagic disease virus
infection
animal infection
non-hemagglutinogenic strain
Opis:
The present paper refers to the cytometric analysis of lymphocytes T (with receptor CD5+), Th (with receptor CD4+), Tc/Ts (with receptor CD8+), lymphocytes CD25+ and lymphocytes B with receptor CD19+ in rabbits experimentally infected with strains of RHD virus - Rainham, Frankfurt and Asturias, not having haemagglutinogenic capacities, which makes them unique, as haemagglutinogenic capacity is a classic and typical property of most strains of this virus. The study was performed in the dynamic system, drawing blood samples from animals at hour 0, namely before the administration of the viral antigen, and then at 4, 8, 12, 24 and 36 h after the infection. The study indicated that Rainham and Asturias strains of RHD virus cause a similar amount of changes as the most immunogenic haemagglutinogenic strains CAMP V-561 and CAMP V-562 of the RHD virus do. In contrast, the Frankfurt strain of the RHD virus is characterised with 5-6-fold lower reactivity in this respect and is most similar to the least immunogenic haemagglutinogenic strain CAMP V-558 of the RHD virus.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 157-162
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phagocytosis of neutrophils in rabbits infected with antigenic variants of RHD (rabbit haemorrhagic disease) virus
Autorzy:
Niedzwiedzka-Rystwej, P.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/31977.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
phagocytosis
neutrophil
rabbit
animal infection
antigenic variant
rabbit hemorrhagic disease virus
hemagglutination
Opis:
The present study was aimed at determining changes in chosen elements of phagocytosis in rabbits infected with 3 antigenic variants of RHD – Hartmannsdorf, Pv97 and 9905, which differed in haemagglutination ability. The animals were tested for phagocytosis parameters, and the results revealed that the examined strains showed the differences. These variations regarded mainly Pv97 strain, as the intensity of the changes were 5 times stronger in comparison to strain Hartmannsdorf and 9905. As all of the strains examined are signified as antigenic variants, we have stated that this feature does not determine their immunological picture. The results suggest the existence of immunological dissimilarities among strains of the RHD virus, which was revealed for the first time in antigenic variants.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lymphocyte subpopulations and apoptosis of immune cells in rabbits experimentally infected with a strain of the RHD virus having a variable haemagglutination capacity
Autorzy:
Niedzwiedzka-Rystwej, P.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/31022.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
lymphocyte subpopulation
apoptosis
immune cell
rabbit
experimental infection
RHD virus
hemagglutination capacity
granulocyte
T cell
B cell
Opis:
The paper describes the immunological response in the matter of percentage of T cells (receptor CD5+) and subpopulations (Th with receptor CD4+, Tc/Ts with receptor CD8+, T with receptor CD25+) and B cells with receptor CD19+, as well as the percentage of apoptotic granulocytes and lymphocytes, in rabbits experimentally infected with the Hagenow strain of the RHD virus. The material chosen for the experiment is special, as among all strains of RHD virus, there are only two strains which carry the variable haemagglutination capacity of red cells. The results of the study show that the Hagenow strain gives an untypical picture of T and B lymphocytes, whereas the results in inducing apoptosis seems to corespond with previous data, confirming the inclusion of apoptosis from 4 h p.i. and the intensity of the phenomenon being higher in granulocytes.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Coagulology, biochemical profile and muscle pathology in calves diagnosed with nutritional muscular dystrophy
Autorzy:
Zarczynska, K.
Baumgartner, W.
Sobiech, P.
Powiązania:
https://bibliotekanauki.pl/articles/31541.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to explain the correlations between selenium deficiency, hemostatic and biochemical disorders, and the progression of pathological changes in calves diagnosed with nutritional muscular dystrophy (NMD). The study was performed on 20 calves with supplementation of 8 ml selenium and vitamin E preparation and 20 calves with symptoms of NMD. Blood was sampled from calves aged 5, 12 and 19 days. On day 19, samples of the biceps femoris muscle were collected from 6 animals in each group for histopathological analysis. The following blood parameters were determined: PLT, PT, TT, APTT, fibrinogen and D-dimer concentrations, antithrombin III activity, glucose, selenium and vitamin E concentrations, activity of CK, LDH and GSH-Px. Muscle sections were stained with H&E and HBFP. Platelet counts were significantly lower in calves with symptoms of NMD. No significant differences in coagulation parameters were observed between the groups. Sick calves were diagnosed with hyperglycemia and elevation of CK and LDH activity. Selenium and vitamin E concentrations in the blood serum were significantly lower in the experimental group together with significant drop in GSH-Px activity. Changes characteristic of Zenker’s necrosis were observed in a muscle of the sick animals. To our best knowledge this is the first study in which the attempt was made to explain the relationship between selenium deficiency and changes in the coagulation system in ruminants.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of two packaging systems on the post-thaw characteristics of canine sperm
Autorzy:
Strzeżek, R.
Polakiewicz, P.
Kordan, W.
Powiązania:
https://bibliotekanauki.pl/articles/31760.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to compare the effect of different packaging systems on some parameters of cryopreserved canine spermatozoa. The experimental material consisted of the sperm-rich fractions of ejaculates collected from four Beagle dogs. Semen samples for cryopreservation were stored in 0.25 ml plastic straws and two aluminum tubes with a total volume of 5.0 ml. Semen was frozen in static nitrogen vapor for 10 minutes (0.25 ml straws) or 15 and 20 minutes (aluminum tubes). Post-thaw assessments involved the determination of sperm motility parameters using a computer assisted sperm analyzer (CASA), sperm plasma membrane integrity (SPMI), mitochondrial membrane potential (MMP) and acrosome integrity (normal apical ridge, NAR). Regardless of the packaging system applied, no significant differences in total sperm motility (TMOT) or selected kinematic parameters were observed after freezing-thawing. However, spermatozoa frozen in 0.25 mL straws were characterized by improved functionality, in particular mitochondrial function, after thawing. The results indicate that large quantities of canine semen can be frozen in aluminum tubes. Further studies are required, however, to evaluate different freezing and thawing rates of aluminum tubes.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Relation between nitric oxide (NO) level in semen and certain properties of boar spermatozoa stored at 17°C
Autorzy:
Orzołek, A.
Zasiadczyk, Ł.
Wysocki, P.
Kordan, W.
Krysztofiak, P.
Powiązania:
https://bibliotekanauki.pl/articles/2087744.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
nitric oxide
liquid storage
boar spermatozoa quality
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 423-426
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Apoptosis of granulocytes and lymphocytes in peripheral blood in rabbits infected with haemagglutinating and non-haemagglutinating antigenic variants of the RHD (rabbit haemorrhagic disease) virus
Autorzy:
Niedzwiedzka-Rystwej, P.
Tokarz-Deptula, B.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/30375.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
This paper attempts to study the dynamics of apoptosis of granulocytes and lymphocytes in peripheral blood in rabbits infected with haemagglutinating (Vt97, Triptis, Hartmannsdorf) and non-haemagglutinating (Pv97, 9905 RHDVa) antigenic variants of the RHD virus. The pathogenicity of those antigenic variants was also assessed by recording the mortality of the infected animals. The animals were infected with antigenic variants and blood was sampled at hour 0,4,8,12,24,36 p.i. and the percentage of apoptotic granulocytes and lymphocytes was measured with the use of flow cytometry. The results of the study showed that apoptosis is included during RHDV infection, as the number of apoptotic granulocytes and lymphocytes increases throughout the experiment; depending on the antigenic variant, apoptosis joins in at 4-8-12 h p.i. and lasts until 24-36 h p.i. Furthermore, the mortality of rabbits infected with the examined strains of RHD virus varied from 30% to 100%. This study performed for the first time in this manner, indicates the importance of apoptosis during infection with the RHD virus.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Autophagy in physiological and pathological processes - selected aspects
Autorzy:
Niedzwiedzka-Rystwej, P.
Tokarz-Deptula, B.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/31271.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
This paper describes a model of cell death, called autophagy, one among other typical and atypical processes of cell death. This phenomenon is present in the organism, from conception until death, and is conditioned by many genes of ATG family, or mTOR kinase and specific proteins, like BNIP3. This process plays a very important role not only in physiological functions of the organism but also in pathological, such as Alzheimer or Huntington disease, as well as diseases caused by viruses.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and identification of zinc ions and heparin binding proteins in yellow fraction of the red deer Cervus Elaphus L. semen
Autorzy:
Orzołek, A.
Dyrda, K.
Rafalska, K.
Wysocki, P.
Kordan, W.
Giżejewski, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087223.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
red deer
yellow fraction
ZnBPs
HBPs
proteomics
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 69-78
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
White and red blood cell picture in rabbits experimentally infected with strains of the rabbit haemorrhagic disease (RHD) virus without or with variable haemagglutination capacity
Autorzy:
Niedzwiedzka-Rastwej, P.
Tokarz-Deptula, B.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/32235.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to establish if haemagglutination of rabbit haemorrhagic disease virus (RHDV) affects haematological picture of peripheral blood in rabbits and the pathogenicity of the virus. The study analyzed white and red blood cell picture in rabbits experimentally infected with two non-haemagglutinating (HA-) RHDV strains (Frankfurt and Asturias) and one strain with variable haemagglutination capacity (HA+/−) (Hagenow). Studies with HA− and HA +/− are rare and relate only to 4 HA− strains (2 RHDV: BLA and Rainham; 2 RHDVa: Pv97 and 9905) and 1 HA+/− RHDV strain: ŻD, where less changes in haematological indices and less pathogenicity were observed. We found that changes caused by HA− Frankfurt strain were related to the number of neutrophils and thrombocytes, while in HA− strain Asturias, in thrombocytes and leukocytes. Changes evoked by HA+/− Hagenow strain pertained to the number of eosinophils, thrombocytes, leukocytes, monocytes, and concentration of hemoglobin. Mortality caused by the Frankfurt strain was 100% between 36 and 48 h post infection (p.i.), while that caused by Asturias strain was 100% between 24 and 36 h p.i., and that observed in case of Hagenow strain was 90% between 36 and 48 h p.i. The changes in haematological picture caused by the HA− and HA+/− RHDV strains were less intensive than those found in case of the HA+ RHDV strains, which cannot be confirmed for pathogenicity, and is not in line with the existing hypothesis suggesting higher pathogenicity in HA+ viruses.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lymphocytes T and B in rabbits infected with RHD virus
Autorzy:
Niedzwiedzka-Rystwej, P.
Tokarz-Deptula, B.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/30261.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to determine the differences in immunological response of animals infected with different antigenic variants of the virus – three haemagglutinating (Vt97, Triptis, Hartmannsdorf) and two non-haemagglutinating (Pv97, 9905 RHDVa). The specific immunological response was measured by the dynamics of changes in the amount of lymphocytes T (with CD5+, CD4+, CD8+, CD25+ receptor) and B (with CD19+ receptor). The study showed differences in immunogenicity of the analysed RHDV antigenic variants, which allowed them to be divided into groups of: more immunogenic strains, including non-haemagglutinating 9905 RHDVa and haemagglutinating Vt97 and Triptis variants; and less immunogenic strains, including the haemagglutinating Hartmannsdorf variant and the non-haemagglutinating Pv97 variant. Such a result may indicate that the agglutination capacity of red blood cells might not be a factor impacting the number of T and B lymphocytes.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thromboelastographic profile of goat blood after the experimental injury of the femoral artery and use of QuikClot gauze and Celox gauze dressings
Autorzy:
Sobiech, P.
Adamiak, Z.
Holak, P.
Jastrzebski, P.
Rogowski, J.
Brzezinski, M.
Bury, K.
Jalynski, M.
Baumgartner, W.
Powiązania:
https://bibliotekanauki.pl/articles/30214.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to evaluate the suitability of thromboelastometry for the analysis of blood test results in goats after the use of hemostatic dressings to control massive bleeding. The study was carried out on 12 goats, 6 animals in each of two subgroups. In all experimental animals incision of the femoral artery was performed, and bleeding was controlled with QuikClot gauze in the first group and Celox gauze in the second group. Dressings were applied for 60 minutes. Blood samples for thromboelastometry were collected from the jugular vein before the incision and 60 min after the application of a dressing. Clotting time (CT), clot formation time (CFT), maximum clot firmness (MCF) and α angle (°) were measured in three standard ROTEM assays (system with generation of reaction curve, numerical parameters and size of the blood clot): intrinsic coagulation pathway (INTEM), extrinsic coagulation pathway (EXTEM) and functional fibrinogen (FIBTEM). Complete hemostasis of the injured femoral artery was found in all goats. No significant differences between pre- and post-incision thromboelastometric parameters were found in any tests in any of the groups, which indicates that the use of dressings was not associated with blood coagulation disorders. This study is the first to describe the use of thromboelastometry in goats for the assessment of clot formation and hemostatic disorders.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Response of the coagulation system after application of hemostatic dressings in an animal model
Autorzy:
Jastrzebski, P.
Adamiak, Z.
Pomianowski, A.
Krystkiewicz, W.
Holak, P.
Sawicki, S.
Przyborowska, P.
Zhalniarovich, Y.
Gudzbeler, G.
Powiązania:
https://bibliotekanauki.pl/articles/30833.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of this study was to determine the response of hemostatic dressings. Coagulation and fibrinolytic systems, red blood cell parameters, platelet and leukocyte counts were evaluated after the application of hemostatic dressings: QuikClot, Chitoauze and Celox gauze. The experiment was performed on ten pigs
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the influence of meloxicam and flunixin meglumine on the apoptosis of peripheral blood CD4plus and CD8plus T cells in calves
Autorzy:
Maslanka, T
Jaroszewski, J.J.
Markiewicz, W.
Jabubowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/31556.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
peripheral blood
T cell
calf
CD4plus T cell
CD8plus T cell
apoptosis
meloxicam
flunixin meglumine
lymphocyte
antiinflammatory drug
Opis:
The aim of the study was to determine whether treatment with recommended doses of meloxicam or flunixin had an effect on the apoptosis of peripheral blood T lymphocytes in calves. The study was carried out on 4-5 months old calves (n = 24, 8 per group). Experimental animals were injected subcutaneously with a single dose of 0.5 mg . kg-1 of meloxicam or intravenously with 3 doses of 2.2 mg . kg-1 day-1 of flunixin. The non-treatment animals served as control. Blood samples were taken at day 0 and at days 1, 2, 3, 5, 7 and 14 after the first NSAIDs injection. Apoptosis was determined by flow cytometry using Annexin V-PE/7-AAD staining. The kinetic analysis of apoptosis in the total lymphocyte population, as well as in the CD4+ and CD8+ subsets did not reveal significant differences in the frequency of early apoptotic cells between control and experimental groups throughout the period studied. Although, 24 h after administration of the first dose of NSAIDs, late-stage apoptosis/necrosis was significantly increased in the total lymphocyte population (the meloxicam group), as well as in the CD4+ (the meloxicam group and the flunixin group) and CD8+ (the flunixin group) subsets of T cells. However, this disturbance was transient, relatively poorly expressed and, thus, unlikely to be of clinical significance. Our results indicate that the use of meloxicam or flunixin in accordance with the recommended dosage regimen in cattle do not have a clinically significant influence on apoptosis of peripheral blood T cells.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 3-12
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
B and T lymphocytes in rabbits change according to the sex and throughout the year
Autorzy:
Niedźwiedzka-Rystwej, P.
Tokarz-Deptuła, B.
Abrantes, J.
Esteves, P.J.
Deptuła, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087335.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rabbit
B and T lymphocytes
reference values
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 37-42
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of matrine on reducing damage to bovine mammary epithelial cells induced by Staphylococcus aureus alpha-hemolysin
Autorzy:
Feng, F.
Ma, W.W.
Luo, H.X.
Guan, C.P.
Zhou, X.Z.
Powiązania:
https://bibliotekanauki.pl/articles/2087720.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
α-hemolysin
BMECs
matrine
cell damage
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 409-413
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hyperglycemia induced C2C12 myoblast cell cycle arrest and skeletal muscle atrophy by modulating sirtuins gene expression in rats
Autorzy:
Surinlert, P.
Thitiphatphuvanon, T.
Khimmaktong, W.
Pholpramool, C.
Tipbunjong, C.
Powiązania:
https://bibliotekanauki.pl/articles/2087134.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
diabetic mellitus
hyperglycemia
muscle atrophy
sirtuins
skeletal muscle
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 563-572
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of hyperbaric oxygen therapy on the healing of postoperative wounds in bitches after hemimastectomy
Autorzy:
Łunkiewicz, W.
Kiełbowicz, Z.
Kalisiak, K.
Tuliński, K.
Prządka, P.
Powiązania:
https://bibliotekanauki.pl/articles/2087261.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
oxygen therapy
ventricle
mastectomy
wound
surgery
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 4; 495-499
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plasma interleukin-1α and interleukin-8 in female dogs with non-metastatic and metastatic malignant mammary gland tumours
Autorzy:
Szczubiał, M.
Łopuszyński, W.
Dąbrowski, R.
Bochniarz, M.
Brodzki, P.
Powiązania:
https://bibliotekanauki.pl/articles/16539196.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
IL-1α
IL-8
canine mammary tumours
plasma
metastases
Opis:
In this study plasma concentrations of IL-1α and IL-8 in 29 female dogs with malignant mammary gland tumours (19 without metastasis and 10 with metastasis) and in 10 healthy control animals were determined. Concentrations of IL-1α and IL-8 were analysed using a specific canine ELISA assay. Mean plasma concentrations of IL-1α and IL-8 were significantly higher (p<0.05) in female dogs with both non-metastatic and metastatic malignant tumours compared to the healthy animals. The concentrations of both tested cytokines were significantly increased (p<0.05) in the dogs with metastasis. In female dogs with mammary carcinomas, the plasma concentration of IL-1α was significantly higher (p<0.05) in the animals with grade 3 tumours compared to the dogs with grade 1 tumours. The concentration of IL-8 was significantly higher (p<0.05) in the dogs with grade 3 tumours compared to that found in the animals with grade 1 and grade 2 tumours. A moderate correlation (r=0.433) was found between IL-1α and IL-8 concentrations in the female dogs. These findings suggest that increased malignancy and invasiveness of canine mammary tumours is associated with an increased production of IL-1α and IL-8 in the tumour microenvironment, which, in turn, leads to an increase in their circulating levels. This may indicate that circulating levels of the cytokines investigated could be considered as diagnostic and prognostic markers in canine malignant mammary tumours. However, further studies in this fields are needed.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 317-324
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of hair clipping on transepidermal water loss values in horses: a pilot study
Autorzy:
Gołyński, M.
Szczepanik, M.P.
Wilkołek, P.M.
Adamek, Ł.R.
Sitkowski, W.
Taszkun, I.
Powiązania:
https://bibliotekanauki.pl/articles/2087774.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
horses
transepidermal water loss
clipping
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 35-38
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Production and characterization of egg yolk antibodies against bovine alimentary tract pathogens
Autorzy:
Sitnik, O.
Jawor, P.
Kopec, W.
Skiba, T.
Stefaniak, T.
Powiązania:
https://bibliotekanauki.pl/articles/32193.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Aim of the study was to evaluate the effect of immunization of hens with bovine vaccines (C, R, T) on the course of IgY antibodies production against selected bovine E.coli strains, rota- and coronaviruses in egg yolk in farm conditions. The hens (40 individuals per group) were vaccinated twice, subcutaneously in four week interval and eggs were harvested once a week. Control group consisted of eggs sampled from non-vaccinated hens located in neighbouring cages. The antibody activity was measured by ELISA. All used vaccines induced the rise of IgY antibody in egg yolks. Based on the duration and the highest level of IgY antibody against bovine alimentary tract pathogens C vaccine was further used in next two trials for vaccination of 1000 hens each time. Double immunization seems to be enough in mounting response against examined pathogens for several weeks. Immunization with C vaccine allowed to harvest eggs with satisfactory levels of E.coli, rotavirus and coronavirus IgY antibodies which may be used to evaluate their protective effect by oral administration in calves.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trueperella pyogenes and Escherichia coli as an etiological factor of endometritis in cows and the susceptibility of these bacteria to selected antibiotics
Autorzy:
Brodzki, P.
Bochniarz, M.
Brodzki, A.
Wrona, Z.
Wawron, W.
Powiązania:
https://bibliotekanauki.pl/articles/32477.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to determine the percentage of participation of particular species of microorganisms, isolated from the uterus of cows with endometritis and from cows without inflammatory lesions of the uterus, in the same postpartum period. The aim of the study was also to examine how long after parturition non-treated endometritis persists. Moreover, antibiotic susceptibility tests were carried out of the bacterial isolates dominating in the uterus. Forty cows were included in the study: 20 cows with endometritis (experimental group) and 20 cows without any inflammatory condition of the uterus (control group). The material for cytological and bacteriological tests was collected on the 5th , 26th , 40th and 60th day after parturition, using an intrauterine brush adapted for cows. The total number of collected isolates was 149, including 120 isolates from the uterus of cows with endometritis and 29 isolates from the uterus of cows without endometritis. The following species of microorganisms were isolated from the material collected from cows with endometritis: T. pyogenes (49.2%), E.coli (22.5%), F. necrophorum (11.7%), Staphylococcus sp. (6.7%), B. melaninogenicus (5.8%), and Streptococcus sp. (4.1%). The participation percentage of particular species of bacteria in the material collected from the uterus of cows without endometritis was as follows: T. pyogenes (27.6%), E.coli (24.2%), Staphylococcus sp. (20.7%), Streptococcus sp. (20.7%), B. melaninogenicus (3.4%) and F. necrophorum (3.4%). The highest percentage of T. pyogenes isolates was susceptible to ceftiofur (89.6%); cefoperazone (85.1%) and amoxicillin combined with clavulanic acid (79.1%). E. coli isolates were most susceptible to amoxicillin combined with clavulanic acid (100%), cefoperazone (94.1%) and oxytetracycline (82.3%).
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of different starch sources on metabolic profile, production and fertility parameters in dairy cows
Autorzy:
Mikula, R.
Nowak, W.
Jaskowski, J.
Mackowiak, P.
Pruszynska Oszmalek, E.
Powiązania:
https://bibliotekanauki.pl/articles/32449.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
starch source
metabolic profile
production
fertility parameter
dairy cow
cow
transition period
maize grain
starch
blood index
fertility
health status
Opis:
The objective of the study was to determine the effect of replacing triticale (high rumen degradable starch) with maize grain (low rumen degradable starch) during the transition period and the first 120 days of lactation on metabolic and hormonal profile indices, milk production and fertility performance in cows. Forty-eight Holstein-Friesian dairy cows were divided into 4 groups: TT (2.5 kg triticale grain/cow per day supplemented from 14 days prepartum to day 120 postpartum), TM (2.5 kg triticale grain/cow per day supplemented from day 14 before parturition to calving, and then 2.5 kg maize grain to 120 days of lactation), MT (2.5 kg maize grain/cow per day supplemented from day 14 before parturition to calving, and then 2.5 kg triticale grain to 120 days of lactation), MM (2.5 kg maize grain/cow per day supplemented from 14 days prepartum to day 120 postpartum). Blood samples were collected 3 weeks and 1 week before calving and on days 14, 56 and 70 of lactation, and they were analyzed in terms of concentrations of glucose, insulin, leptin, insulin-like growth factor I, nonesterified fatty acids, triglycerides, cholesterol, blood urea nitrogen and activities of aspartate aminotransferase and gamma glutamyl transpeptidase. Milk samples were collected twice a day at weekly intervals and analyzed for fat, protein and lactose. Milk yield and individual dry mater intake were recorded at weekly intervals. Body condition was estimated 3 weeks before calving, on parturition day and on days 14, 56 and 120 of lactation. Replacing triticale grain with maize grain in the transition period and during lactation positively affected fertility of lactating cows. An increased first service conception rate and shortening of the days open period was observed in MM and TM groups in comparison to those found in group MT (P ≤ 0.05). The lowest number of services per conception was recorded in groups MM and TM (P ≤ 0.05). Although the impact of milk production and the most of the blood indices were not significantly affected by this treatment, the results of the study suggest that maize grain in the transition period and lactation might be a more effective energy source for dairy cows than triticale grain.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Histopathology in diagnosis of broiler chicken and layer diseases – review of cases 1999-2010
Autorzy:
Dolka, I.
Sapierzynski, R.
Bielecki, W.
Malicka, E.
Zbikowski, A.
Szeleszczuk, P.
Powiązania:
https://bibliotekanauki.pl/articles/32185.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to estimate the prevalence of histopathological lesions in the different organs in relation to the commercial-type and the age of birds (i.e. broiler chickens and layers). During the period 1999-2010 a total of 189 cases was submitted to the Division of Animal Pathomorphology, Department of Pathology and Veterinary Diagnostics at WULS. Most cases were found in broiler chickens (66.7%). The majority of the histopathological lesions were detected in the liver and lymphoid organs. In of 29% cases of hepatic injury pathognomonic lesions associated with inclusion body hepatitis (IBH) were found. The mean age of birds was 23 days. Among IBH cases proventriculitis (58%) was more often found than gizzard lesions (25.8%). Interestingly, we noted some intranuclear inclusions in the epithelial cells within the proventriculus. A low percentage of histopathological evidence of infectious bursal disease (IBD) was reported in chickens. The gastrointestinal tract was the second most frequent predilection site for histopathological lesions. Histopathological findings within the heart and lungs were less common and were more often seen in the upper respiratory tract. Cases of infectious laryngotracheitis (ILT) were registered in broiler chickens (3.2%, mean age 37 days) and in layers (4.8%; mean age 196 days). Lesions associated with Marek’s disease, avian leukosis and fowl pox were recognized only in layers, respectively in 3.2% (mean age 176 days), 1.6% (mean age 205 days) and 1.1% (mean age 196 days) of all cases. Avian encephalomyelitis (AE) was noted only in 0.5% of all cases.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression analysis of multifunctional RNA-binding protein hnRNP K during development of mammalian testis
Autorzy:
Xu, H.
Zhang, P.
Li, R.
Wu, W.
Wang, S.
Xu, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087724.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
hnRNP K
testis development
spermatogenesis
pig
rat
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 343-352
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiological Study of Canine Mast Cell Tumours According to the Histological Malignancy Grade
Autorzy:
Śmiech, Anna
Jasik, A.
Ślaska, B.
Łopuszyński, W.
Szczepanik, M.
Wilkołek, P.
Powiązania:
https://bibliotekanauki.pl/articles/2087901.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Polish Journal of Veterinary Sciences; 2017, 3; 455-465
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Germocyte lesions caused by porcine circovirus type 2b in Kunming mice
Autorzy:
Yuan, A.W.
Pang, P.
Gong, Q.L.
Deng, Z.B.
Powiązania:
https://bibliotekanauki.pl/articles/31710.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Porcine circovirus type 2 (PCV2) has been known as a causative agent of reproductive failure in the sow. In the present study mouse model was used to investigate PCV2 infection. In order to investigate whether PCV2 can induce lesions of spermatocytes and oocytes, 6 male and 6 female mice were each inoculated intraperitoneally with PCV2b, and 3 male and 3 female mice mock-infected with cell culture supernatant served as controls. Samples of testes and ovaries from PCV2b-inoculated and mock-infected mice were investigated using PCR, histopathological, ultrastructural and immunofluorescent histochemical methods at 14 and 21 day post infection (dpi). The study revealed that in the virus-inoculated mice leydig cells in testes and granulosa cells in ovaries were degenerated, and a small number of spermatocytes and oocytes showed apoptosis. Positive PCV2b antigen signals were also observed in these apoptotic cells. It can be suggested that PCV2 can cause lesions of spermatocyte and oocyte prior to zygotes formation in its host.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Transepidermal water loss and skin hydration in healthy cats and cats with non-flea non-food hypersensitivity dermatitis (NFNFHD)
Autorzy:
Szczepanik, M.P.
Wilkołek, P.M.
Adamek, Ł.R.
Kalisz, G.
Gołyński, M.
Sitkowski, W.
Taszkun, I.
Powiązania:
https://bibliotekanauki.pl/articles/2087561.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cats
atopic dermatitis
transepidermal water loss
skin hydration
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 237-242
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The examination of biophysical skin parameters (transepidermal water loss, skin hydration and pH value) in different body regions in Polish ponies
Autorzy:
Szczepanik, M P
Wilkolek, P M
Pluta, M.
Adamek, L R
Golynski, M
Pomorski, Z J H
Sitkowski, W
Powiązania:
https://bibliotekanauki.pl/articles/31823.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The purpose of this study was to evaluate transepidermal water loss, skin hydration and skin pH in normal polish ponies. Twelve ponies of both sexes were examined in the study. Measurements were taken from seven different sites: the neck region, the shoulder, thorax, lumbar, inguinal, lip region and the pinna. In each of the regions transepidermal water loss (TEWL), skin hydration and skin pH were measured. For transepidermal water loss, the lowest values were observed in the pinna (10.54 g/hm²), while the highest values were observed in the lip region (30.98 g/hm²). In the case of skin hydration the lowest values were observed for the thorax region (1.96 CU), and the highest for the lip region (48.28 CU). For skin pH, the lowest results were obtained in the pinna (7.03), and the highest in the lumbar region (8.05).
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zebrafish: an animal model for research in veterinary medicine
Autorzy:
Nowik, N.
Podlasz, P.
Jakimiuk, A.
Kasica, N.
Sienkiewicz, W.
Kaleczyc, J.
Powiązania:
https://bibliotekanauki.pl/articles/31997.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The zebrafish (Danio rerio) has become known as an excellent model organism for studies of vertebrate biology, vertebrate genetics, embryonal development, diseases and drug screening. Nevertheless, there is still lack of detailed reports about usage of the zebrafish as a model in veterinary medicine. Comparing to other vertebrates, they can lay hundreds of eggs at weekly intervals, externally fertilized zebrafish embryos are accessible to observation and manipulation at all stages of their development, which makes possible to simplify the research techniques such as fate mapping, fluorescent tracer time-lapse lineage analysis and single cell transplantation. Although zebrafish are only 2.5 cm long, they are easy to maintain. Intraperitoneal and intracerebroventricular injections, blood sampling and measurement of food intake are possible to be carry out in adult zebrafish. Danio rerio is a useful animal model for neurobiology, developmental biology, drug research, virology, microbiology and genetics. A lot of diseases, for which the zebrafish is a perfect model organism, affect aquatic animals. For a part of them, like those caused by Mycobacterium marinum or Pseudoloma neutrophila, Danio rerio is a natural host, but the zebrafish is also susceptible to the most of fish diseases including Itch, Spring viraemia of carp and Infectious spleen and kidney necrosis. The zebrafish is commonly used in research of bacterial virulence. The zebrafish embryo allows for rapid, non-invasive and real time analysis of bacterial infections in a vertebrate host. Plenty of common pathogens can be examined using zebrafish model: Streptococcus iniae, Vibrio anguillarum or Listeria monocytogenes. The steps are taken to use the zebrafish also in fungal research, especially that dealing with Candida albicans and Cryptococcus neoformans. Although, the zebrafish is used commonly as an animal model to study diseases caused by external agents, it is also useful in studies of metabolic disorders including fatty liver disease and diabetes. The zebrafish is also a valuable tool as a model in behavioral studies connected with feeding, predator evasion, habituation and memory or lateralized control of behavior. The aim of the present article is to familiarize the reader with the possibilities of Danio rerio as an experimental model for veterinary medicine.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plasma humanin as a prognostic biomarker for canine myxomatous mitral valve disease: a comparison with plasma NT-roBNP
Autorzy:
Mangkhang, K.
Punyapornwithaya, V.
Tankaew, P.
Pongkan, W.
Chattipakorn, N.
Boonyapakorn, C.
Powiązania:
https://bibliotekanauki.pl/articles/2087638.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Humanin
myxomatous mitral valve disease
heart failure
NT-proBNP
ROS
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 4; 673-680
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Real time PCR detection of rabbit haemorrhagic disease virus in rabbits infected with different European strains of RHDV
Autorzy:
Niedzwiedzka-Rystwej, P.
Hukowska-Szematowicz, B.
Dzialo, J.
Tokarz-Deptula, B.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/32493.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The paper concerns the use of a novel, very effective diagnostic method, a real-time PCR for diagnosis of a viral agent causing viral haemorrhagic disease in rabbits – RHDV. Until now, the method was widely used for detecting many different viruses, both DNA, and RNA, but as far as RHDV is concerned, there are not many records of such use. This study aimed at the detection of 17 different strains from different European regions, differing in biological features and mortality. The study confirmed that real-time PCR is an applicable and effective method for diagnosis of RHDV, irrespective of the stains’ features.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus)
Autorzy:
Bancerz-Kisiel, A.
Szczerba-Turek, A.
Platt-Samoraj, A.
Socha, P.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087938.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Free-living animals are an important environmental reservoir of pathogens dangerous for other animal species and humans. One of those is Yersinia (Y.) enterocolitica, the causative agent of yersiniosis - foodborne, enzootic disease, significant for public health. The purpose of the study was to identify bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus) obtained during the 2010/2011 hunting season in north-eastern Poland. From among 48 rectal swabs obtained from 24 roe deer, two strains of Y. enterocolitica from one animal were isolated. Although both belonged to biotype 1A they were identified as different serotypes. The strain obtained from cold culture (PSB) belonged to serotype O:5, while the strain isolated from warm culture (ITC) was regarded as nonidentified (NI), what may suggest mixed infection in that animal. The presence of ystB gene, coding for YstB enterotoxin, directly related to Y. enterocolitica pathogenicity was detected in both strains using triplex PCR. The effect of the examination of 32 swabs obtained from 16 red deer was the isolation of two Y. enterocolitica strains from two different animals. Both belonged to biotype 1A with NI serotype, but were originated from different types of culture. They gave positive results in case of products of a size corresponding to the ystB gene. No amplicons corresponding to ail and ystA genes were found. Roe deer and red deer may carry and shed Y. enterocolitica, what seems to be important in aspect of an environmental reservoir of this pathogen. The Y. enterocolitica strains isolated from wild ruminants had the amplicons of the ystB gene, what suggest they can be potential source of Y. enterocolitica infection for humans.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 315-319
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of horse breed on transepidermal water loss
Autorzy:
Szczepanik, M.P.
Wilkolek, P.M.
Adamek, L.R.
Pluta, M.
Golynski, M.
Sitkowski, W.
Kalisz, G.
Taszkun, I.
Pomorski, Z.J.K.
Powiązania:
https://bibliotekanauki.pl/articles/30727.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Non-invasive methods of skin condition assessment include, among others, the evaluation of transepidermal water loss (TEWL). The aim of the study was to examine whether TEWL values depend on horse breed. The study was conducted on four breeds: “Felin” ponies (FP) (n=16), Polish koniks (PK) (n=15), Polish cold-blooded horses (PcbH) (n=11) and Wielkopolska horses (WH) (n=12). It was found that horse breed influences TEWL values. In the neck region, statistically significant differences were found between PK and FP (p=0.006), and PK and WH (p=0.0005). In the lumbar region, there were statistically significant differences between FP and PK (p=0.0009), FP and PcbH (p=0.0016) as well as between PK and WH (p=0.000037), and PcbH and WH (p=0.0006). In the inguinal region statistically significant differences were found between FP and PK (p=0.0003), FP and PcbH (p=0.0005), PK and WH (p=0.009) and PcbH and WH (p=0.006). In the lip region statistically significant differences were observed between FP and PK (p=0.013) as well as between PK and PcbH (p=0.029) and PK and WH (p=0.009). In the examination of TEWL animal breed should be taken into consideration. The non-significant differences found in three of the examined body regions may suggest that these regions are the most adequate for TEWL assessment.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in blood acid-base balance parameters and coagulation profile during diarrhea in calves
Autorzy:
Sobiech, P.
Rekawek, W.
Ali, M.
Targonski, R.
Zarczynska, K.
Snarska, A.
Stopyra, A.
Powiązania:
https://bibliotekanauki.pl/articles/31605.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The purpose of this study was to investigate possible alterations in acid-base balance parameters and the coagulation profile in neonatal diarrheic calves. Twenty neonatal diarrheic and 20 clinically healthy neonatal calves aged between 1 week to 10 days were used. All blood samples were taken on the third day from the onset of diarrhea symptom. Venous blood samples were collected from each animal to determine platelet numbers, pH, pCO₂, pO₂, HCO₃⁻, BE, O₂SAT, ctCO₂ and electrolytes (K⁺, Na⁺ and Cl⁻). Plasma samples were collected from each animal for the measurement of prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), the concentrations of fibrinogen, D-dimer and the activity of antithrombin III (AT III). Blood pH (7.19), BE (-10.6 mmol/l), HCO₃⁻ (25.15 mmol/l), pO₂ (3.33 kPa), O₂SAT (24.12 %) were significantly lower and serum concentration of K⁺ (6.55 mmol/l) was significantly higher in diarrheic calves. These changes indicate the state of uncompensated metabolic acidosis with accompanying hyperkalemia. TT (32.05s) and APTT (39.9s) values were more prolonged in calves with diarrhea than in the control group. D-dimer (587.25 μg/l) concentrations were significantly increased while a visible drop in AT III (103.75%) activity and platelets counts (598 x10⁹/l) were observed in diarrheic group of calves. The results suggest that a consumptive type of disseminated intravascular coagulation (DIC) developed in diarrheic calves.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The impact of cow nutrition in the dry period on colostrum quality and immune status of calves
Autorzy:
Nowak, W.
Mikula, R.
Zachwieja, A.
Paczynska, K.
Pecka, E.
Drzazga, K.
Slosarz, P.
Powiązania:
https://bibliotekanauki.pl/articles/31995.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cow
animal nutrition
dry period
colostrum
quality
calf
immunological status
immunoglobulin
insulin-like growth factor 1
Opis:
The objective of the study was to evaluate the effect of energy contents in a dry period diets in “7+1” feeding strategy of dry cow including 7 weeks of the dry period (far-off) and 1 week of a close-up period, on colostrum quality and the immune status of calves. Forty Holstein multiparous cows were dried at 56d before the expected date of calving and were assigned to the higher energy diet group (HE; 0.69 UFL/kg DM, NDF 52% DM), or the lower energy diet group (LE; 0.61 UFL/1kg DM, NDF 56% DM). From -7d to the expected calving date up to 21 d of lactation, all cows were fed the same fresh, lactation diet. Samples of colostrum were collected within 2h after parturition and its density, dry matter content and concentrations of immunoglobulins, fat, protein, lactose, urea and somatic cell counts were measured. Calves were weighed 2 h after calving and on the 21d of life. On 3d and 21d of calves’ life blood samples were collected and the concentration of IgG, IgA, IgM, insulin-like growth factor 1 (IGF-1) as well as total protein and albumin concentrations were determined. Treatments had no significant effect on composition of colostrum and serum immunoglobulins and IGF-1 concentration. In both groups the weight of calves at birth was similar, still daily body weight gain during 21 day of rearing period in HE group was higher than in the LE group (P=0.05).
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution and chemical coding patterns of cocaine- and amphetaminer-egulated transcript - like immunoreactive (CART - LI) neurons in the enteric nervous system of the porcine stomach cardia
Autorzy:
Rekawek, W.
Sobiech, P.
Gonkowski, S.
Zarczynska, K.
Snarska, A.
Wasniewski, T.
Wojtkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/30441.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to determine the presence of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) neurons and co-localisation of CART with vesicular acetylcholine transporter (VAChT), neuronal nitric oxide synthase (n-NOS), vasoactive intestinal polypeptide (VIP), substance P (SP) and leu-enkephalin (LENK) in the enteric nervous system of the porcine gastric cardia by using a double-labelling immunofluorescence technique. CART-LI neurons were observed in the myenteric plexus (18.2}2.6%). A dense network of CART-LI nerve fibers was mainly observed in the muscular layer. CART showed co-localization mainly with VAChT, n-NOS, VIP and to a lesser degree with LENK and SP. Distribution of CART and its co- ocalization with other neurotransmitters suggest that this peptide plays an important role in gastric motility in the pig.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Values of apoptosis of lymphocytes and granulocytes in peripheral blood of Polish mixed-breed rabbits in the annual cycle
Autorzy:
Tokarz-Deptula, D.
Hukowska-Szematowicz, B.
Niedzwidzka-Rystwej, P.
Trzeciak-Ryczek, A.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/31009.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of the study was to determine values of apoptosis for peripheral blood lymphocytes and granulocytes, including dependency on the season of the year, in Polish mixed-breed rabbits and in mixed-breed rabbits with the addition of blood of meat-breed rabbits. The study was carried out in four seasons of the year (spring, summer, autumn, winter) involving 120 Polish mixed-breed rabbits – group I, and 120 Polish mixed-breed rabbits with addition of meat-breed rabbit blood – group II. Assessment of apoptosis of lymphocytes and granulocytes was performed using as FACScan cytometer by Becton Dickinson with FACSDiva software (USA), using as ApoFluor®Green Caspase reagent kit (MP Biomedicals, USA) to detect the activity of the total caspase pool 1, 3, 4, 5, 6, 7, 8, 9 in granulocytes and lymphocytes of rabbit peripheral blood. The results for apoptosis of lymphocytes and granulocytes in peripheral blood in the animals investigated (group I and II) were subjected to statistical analysis with the t’Student test at p=0.05. It was noted that, in rabbits from group I, the values for apoptosis of lymphocytes were the highest in winter and autumn (36.02% and 31.24%, respectively), and the lowest in spring and summer (26.73% and 22.72%, respectively), whereas in the case of granulocytes the highest values were in summer and spring (14.69% and 12.95%, respectively), and the lowest in winter and autumn (8.16% and 8.57%, respectively). In mixed-breed rabbits with the addition of meat-breed blood (group II), the values for apoptosis of lympocytes were the highest in spring (29.13%), and the lowest in summer (25.43%); whereas in the case of granulocytes the highest values were in summer and spring (14.0% and 11.15%, respectively), and the lowest in autumn and winter (7.46% and 7.64%, respectively).
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of serum levels of allergen-specific immunoglobulin E in different seasons and breeds in healthy horses
Autorzy:
Wilkolek, P.M.
Pomorski, Z.J.H.
Szczepanik, M.P.
Adamek, L.
Pluta, M.
Taszkun, I.
Golynski, M.
Rozwod, A.
Sitkowski, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087936.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The present study was designed to asses specific IgE towards environment allergens in 42 healthy horses. Determination of this immunoglobulin in serum serve as diagnostic tools in allergic diseases to improve efficacy of the treatment and proper allergen selection to specific immunotherapy. Serum levels of allergen specific IgE were measured with equine monoclonal antibody, using 15 individual and 5 mix allergens in North European Panel. The study revealed season dependent increased levels of allergen specific IgE in normal horses. It is noteworthy that healthy horses show high percentage of positive reactions, most commonly towards to domestic mites D. farinae (80%), D. pteronyssinus (35.71%) and storage mites T. putrenscentiae (42.86%), Acarus siro (40.48%). These allergens play an important role in equine, canine and feline atopic dermatitis. We also demonstrated high IgE levels in the group of horse specific insect allergens. Tabanus sp. (35.71%), Culicoides sp. (28.57%) and Simulium sp. (26.19%) were the most frequent insect positive reaction allergens. No positive reactions in all groups of allergens were found in winter season, low and merely detectable levels of antibodies have been found relating to D. farianae and T. putrescentiae allergen. We observed elevated mould-IgE levels in horses that live in stables, while outdoor living horses showed very low levels. Amongst all positive reactions we observed only weak and moderate reactions but no strong positive reactions were found. No significant differences were observed between three breeds of horses with the exception of moulds and D. pteronyssinus allergens.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 331-337
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hematological parameters in Polish mixed breed rabbits with addition of meat breed blood in the annual cycle
Autorzy:
Tokarz-Deptula, B.
Niedzwiecka-Rystwej, P.
Adamiak, M.
Hukowska-Szematowicz, B.
Trzeciak-Ryczek, A.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/30719.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In the paper we studied haematologic values,such as haemoglobin concentration, haematocrit value, thrombocytes, leucocytes: lymphocytes, neutrophils, basophils, eosinophils and monocytes in the pheral blood in Polish mixed-breeds with addition of meat breed blood in order to obtain the reference values which are until now not available for this animals. In studying this indices we took into consideration the impact of the season (spring, summer, autumn, winter), and sex of the animals. The studies have shown a high impact of the season of the year on those rabbits, but only in spring and summer. Moreover we observed that the sex has mean impact on the studied values of haematological parameters in those rabbits. According to our knowledge, this is the first paper on haematologic values in this widely used group of rabbits, so they may serve as reference values.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
B-cell and T-cell values in peripheral blood in Polish mixed-breed rabbits with addition of blood of meet breeds
Autorzy:
Tokarz-Deptula, B.
Niedzwiedzka-Rystwej, P.
Hukowska-Szematowicz, B.
Adamiak, M.
Trzeciak-Ryczek, A.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/30822.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In Poland, rabbit is a highly valued animal, due to dietetic and flavour values of its meat, but above all, rabbits tend to be commonly used laboratory animals. The aim of the study was developing standards for counts of B-cells with CD19+ receptor, T-cells with CD5+ receptor, and their subpopulations, namely T-cells with CD4+, CD8+ and CD25+ receptor in the peripheral blood of mixed-breed Polish rabbits with addition of blood of meet breeds, including the assessment of the impact of four seasons of the year and animal sex on the values of the immunological parameters determined. The results showed that the counts of B- and T-cells and their subpopulations in peripheral blood remain within the following ranges: for CD19+ B-cells: 1.05 - 3.05%, for CD5+ T-cells: 34.00 - 43.07%, CD4+ T-cells: 23.52 - 33.23%, CD8+ T-cells: 12.55 - 17.30%, whereas for CD25+ T-cells: 0.72 - 2.81%. As it comes to the season of the year, it was observed that it principally affects the values of CD25+ T-cells, while in the case of rabbit sex, more changes were found in females.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of serum concentrations of environmental allergen-specific IgE in atopic and healthy (nonatopic) horses
Autorzy:
Gołyński, M.
Wilkołek, P.
Sitkowski, W.
Szczepanik, M.
Adamek, Ł.
Pluta, M.
Taszkun, I.
Malinowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087865.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
IgE
equine atopic dermatitis
healthy horses
immunology
allergens
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 789-794
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Natural immunity factors in Polish mixed breed rabbits
Autorzy:
Tokarz-Deptula, B.
Niedzwiedzka-Rystwej, P.
Adamiak, M.
Hukowska-Szematowicz, B.
Trzeciak-Ryczek, A.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087985.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Mixed-breed rabbits in Poland are widely used for diagnostic and scientific research and as utility animals, therefore there is a need to know their immunological status, as well as their haematological status. In this study natural immunity factors were analyzed in Polish mixed-breed rabbits and Polish mixed-breed rabbits with addition of blood of meet-breed, considering the impact of sex and season of the year (spring, summer, autumn, winter) using measurement of non-specific cellular and humoral immunity parameters in peripheral blood. The study has revealed that there is a variety between the two commonly used mixed-breed types of rabbits, especially when sex and season is concerned, which is crucial for using these animals in experiments.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dose-dependent in vivo effect of Rhodiola and Echinacea on the mitogen-induced lymphocyte proliferation in mice
Autorzy:
Skopińska-Rozewska, E.
Sokolnicka, I.
Siwicki, A.K.
Stankiewicz, W.
Dabrowski, M.P.
Buchwald, W.
Krajewska-Patan, A.
Mielcarek, S.
Mscisz, A.
Furmanowa, M.
Powiązania:
https://bibliotekanauki.pl/articles/31333.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
in vivo effect
Rhodiola rosea
Echinacea purpurea
Echinacea angustifolia
Rhodiola quadrifida
mitogen-induced lymphocyte
lymphocyte
proliferation
mice
mouse
Opis:
Echinacea purpurea (EP) and Echinacea angustifolia (EA) are ones of the most important world’s herbs with immunotropic activity. They were traditional medicinal plants used by North American Indians for the treatment of various illnesses. Now they are cultivated in many countries and are used mainly to treat respiratory tract infections. Rhodiola rosea (RR) and Rhodiola quadrifida (RQ) are medicinal plants originated from Asia and used traditionally as adaptogens, antidepressants, and anti-inflammatory remedies. We previously reported, that extracts of underground parts of RR and RQ exhibited immunotropic activity. We have demonstrated in pigs that in vitro RR or RQ supplementation of blood lymphocyte cultures stimulated T cell proliferative response to Con A in lower, and inhibited it in higher Rhodiola extract concentrations. The aim of this work was to evaluate the in vivo effect of these herbal remedies on the in vitro proliferative response of mouse splenic lymphocytes to another T-cell mitogen- Phaseolus vulgaris haemagglutinin (PHA). We have found significant stimulation of proliferative response, in comparison to the controls, in mice fed lower doses of tested remedies, and inhibition, no effect or lower stimulation, in mice fed higher doses of these drugs.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Involvement of the different extracts from roots of Salvia miltiorrhiza Bunge on acute hypobaric hypoxia-induced cardiovascular effects in rats – preliminary report
Autorzy:
Buchwald, W.
Mikolajczak, P.L.
Krajewska-Patan, A.
Dreger, M.
Gorska-Paukszta, M.
Szulc, M.
Polcyn, P.
Piorunska-Mikolajczak, A.
Mielcarek, S.
Czerny, B.
Mrozikiewicz, P.M.
Bobkiewicz-Kozlowska, T.
Powiązania:
https://bibliotekanauki.pl/articles/31897.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The present study was carried out to investigate the protective effects of roots of Salvia miltiorrhiza Bunge on hypobaric hypoxia. Two extracts of S. miltiorrhiza (extract 1: ethanol : water - 50 : 50; extract 2: 96% ethanol) were used. The experiments were performed after 7 consecutive days of administration of the extracts (200 mg/kg b.w., intragastrically) to male Wistar rats. Next, after placing animals for 60 min in the controlled acute hypobaric hypoxia (500 mm Hg) the systolic arterial blood pressure (SAP) in conscious rats, bioelectric heart activity in unconscious rats and analysis of oxidative stress parameters in the blood of rats: malonyldialdehyde (MDA) and lipid peroxidase (LPO) concentration, activity of superoxide dismutase (SOD) or glutathione peroxidase (GPX) were assayed. It was found out that the extract 1 augmented the lowering of SAP shown in hypoxia affected control rats. On the contrary the extract 2 reversed SAP to values obtained in control animals. Moreover, both extracts led to the normalization of hypoxia-induced tachycardia and levels of MDA, LPO and SOD. It seems that the above-mentioned effects are coupled with different active compounds content in the extracts, however more studies are needed to confirm this hypothesis.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The evaluation of selected parameters of cellular nonspecific immunity in normal and allergic horses
Autorzy:
Wilkolek, P.
Szczepanik, M.
Golynski, M.
Adamek, L.
Pomorska, A.
Maj-Martyniuk, M.
Sitkowski, W.
Powiązania:
https://bibliotekanauki.pl/articles/31773.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
selected parameter
cellular immunity
non-specific immunity
healthy animal
diseased animal
allergy
horse
animal disease
phagocytosis
Opis:
The main aim of this study was to compare selected nonspecific immunity parameters in 14 allergic and 12 healthy horses. Each animal was assessed according to the following parameters: in vitro functional capacity of phagocytic cells using the nitro blue tetrazolium chloride reduction test, both spontaneous (NBT) and zymozan stimulated (NBTs), and ingestion capacity of phagocytic cells using a phagocytic index test (IF) and percentage of phagocytosing neutrophils activity (%KF). Differences were demonstrated between the group of allergic horses, especially with severe allergy symptoms, and healthy horses in NBTs values, with higher values in healthy horses. The values of the phagocytic index were significantly higher in horses with allergy.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Values of white and red blood cell parameters in Polish mixed breed rabbits in the annual cycle
Autorzy:
Tokarz-Deptula, .
Niedzwiecka-Rystwej, P.
Adamiak, M.
Hukowska-Szematowicz, B.
Trzeciak-Ryczek, A.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/32430.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to develop reference values for such haematologic values as haemoglobin concentration, haematocrit value, volume of erythrocytes, thrombocytes, leucocytes, lymphocytes, neutrophils, basophils, eosinophils and monocytes in peripheral blood in Polish mixed-breed rabbits, considering the impact of the season (spring, summer, autumn, winter), and sex of the animals. The results reveal, that the season of the year has a small impact in Polish mixed-breed rabbits, and it principally referred to spring and summer. Finally it was shown, that sex has average impact on the studied values of haematological parameters.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Relationship between pH of ruminal fluid during subacute ruminal acidosis and physiological response of the Polish Holstein-Friesian dairy cows
Autorzy:
Stefańska, B.
Pruszyńska-Oszmałek, E.
Szczepankiewicz, D.
Stajek, K.
Stefański, P.
Gehrke, M.
Nowak, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087914.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Polish Journal of Veterinary Sciences; 2017, 3; 551-558
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of doxazosin on the contractility of the urinary bladder in female pigs with experimentally induced cystitis
Autorzy:
Jaroszewski, J.J.
Markiewicz, W.
Bossowska, A.
Lepiarczyk, E.
Majewski, M.
Radziszewski, P.
Wiśniewska, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087928.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Polish Journal of Veterinary Sciences; 2017, 3; 485-490
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line
Autorzy:
Cymerys, J.
Slonska, A.
Brzezicka, J.
Tucholska, A.
Chmielewska, A.
Rola, J.
Malik, P.
Banbura, M.W.
Powiązania:
https://bibliotekanauki.pl/articles/32263.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Equine herpesvirus type 1 (EHV-1) causes respiratory infections, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single-point mutation in DNA polymerase gene, resulting in an amino acid variation (N752/D752), is significantly associated with the neuropathogenic potential of EHV-1 strains. The aim of the study was to elucidate if there are any differences between neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains in their ability to infect neuronal cells. For the tested EHV-1 strains, cytopathic effect (CPE) was manifested by changed morphology of cells, destruction of actin cytoskeleton and nuclei degeneration, which led to focal degeneration. Moreover, EHV-1 26 strain caused fusion of the infected cells to form syncytia in culture. Real-time PCR analysis demonstrated that both neuropathogenic and non-neuropathogenic EHV-1 strains replicated in neurons and ED cells (equine dermal cell line) at a similar level. We can assume that a point mutation in the EHV-1 polymerase does not affect viral replication in this cell type.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antimicrobial activity of some plant extracts against bacterial pathogens isolated from faeces of red deer (Cervus elaphus)
Autorzy:
Gnat, S.
Zięba, P.
Majer-Dziedzic, B.
Nowakiewicz, A.
Trościańczyk, A.
Ziółkowska, G.
Jesionek, W.
Choma, I.
Dziedzic, R.
Powiązania:
https://bibliotekanauki.pl/articles/2087857.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 697-706
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Endogenous carbon monoxide (CO) as a modulating factor of molecular biological clock in the hypothalamic structures involved in light transmission in pig and wild boar hybrid during long and short day season
Autorzy:
Gilun, P.
Koziorowska-Gilun, M.
Wąsowska, B.
Sowa-Kućma, M.
Kozioł, K.
Romerowicz-Misielak, M.
Kordan, W.
Koziorowski, M.
Powiązania:
https://bibliotekanauki.pl/articles/16647460.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
carbon monoxide
humoral pathway
light
master biological clock
seasonal
Opis:
Mature males of a wild boar-pig crossbreed, during the long and short day season, were used for the study which demonstrates that the chemical light carrier CO regulates the expression of biological clock genes in the hypothalamus via humoral pathways. Autologous blood with experimentally elevated concentrations of endogenous CO (using lamps with white light-emitting diodes) was infused into the ophthalmic venous sinus via the right dorsal nasal vein. Molecular biology methods: qPCR and Western Blot were used to determine the expression of genes and biological clock proteins. The results showed that elevated endogenous CO levels, through blood irradiation, induces changes in genes expression involved in the functioning of the main biological clock located in suprachiasmatic nuclei. Changes in the expression of the transcription factors Bmal1, Clock and Npas2 have a similar pattern in both structures, where a very large decrease in gene expression was shown after exposure to elevated endogenous CO levels. The changes in the gene expression of PER 1-2, CRY 1-2, and REV-ERB α-β and ROR β are not the same for both POA and DH hypothalamic structures, indicating that both structures respond differently to the humoral signal received. The results indicate that CO is a chemical light molecule whose production in an organism depends on the amount of light. An adequate amount of light is an essential factor for the proper functioning of the main biological clock.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 65-82
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dietary energy density in the dry period on the metabolic status of lactating cows
Autorzy:
Nowak, W.
Mikula, R.
Pruszynska-Oszmalek, E.
Mackowiak, P.
Stefanska, B.
Kasprowicz-Potocka, M.
Frankiewicz, A
Drzazga, K.
Powiązania:
https://bibliotekanauki.pl/articles/31038.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to investigate the effect of different energy concentrations in the isonitrogenous diet fed during the dry period on postpartum health, fertility and blood variables. Forty Holstein multiparous cows were dried 56 days before the expected day of calving and assigned to group (M) with moderate energy concentrations of 0.69 UFL/kg DM or to the low-energy group (L) with energy density of 0.61 UFL/kg DM. From the 7d before the expected day of calving until the 21d of lactation, all the cows were fed the same fresh transition diet (0.82 UFL/kg DM). From the 22d to the 90d of lactation, all the cows received the same highest energy-density lactation diet (0.90 UFL/kg DM). During the dry period the decline of BCS in groups M and L were 0.07 and 0.12 units respectively. The average decrease of BCS from calving to 56 d of lactation were the same in both experimental groups (0.21 BCS). The first-service conception rate tended to be higher in the M group. Insulin-like growth factor-1, glucose, β-hydroxybutyric acid, non-esterified fatty acid, thyroxine serum concentrations prepartum and 3 and 5d postpartum were not significantly affected by the treatment in the dry period.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of coagulase-negative staphylococci isolated from milk of cows with subclinical mastitis
Autorzy:
Banach, T.
Bocniarz, M.
Lyp, P.
Adaszek, L.
Wawron, W.
Furmaga, B.
Skrzypczak, M.
Zietek, J.
Winiarczyk, S.
Powiązania:
https://bibliotekanauki.pl/articles/31177.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to use matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of coagulase-negative staphylococci (CNS) isolated from the milk of cows with subclinical mastitis. The study material consisted of 33 isolates of CNS, identified by the results of API Staph tests, obtained from the milk of cows with subclinical mastitis. Based on the spectra analyses, MALDI-TOF MS tests of 33 bacterial samples allowed identification of the microorganisms in 27 cases (81.8%). The most frequent cause of subclinical mastitis was found to be Staphylococcus sciuri (39%), while S. vitulinus was detected in 15% of the milk samples. The results obtained indicate that MALDI-TOF MS can be used for the identification of CNS isolated from bovine mastitis as a method supplementary to biochemical tests.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of short-term rapid ventricular pacing followed by pacing interruption on arterial blood pressure in healthy pigs and pigs with tachycardiomyopathy
Autorzy:
Skrzypczak, P.
Zysko, D.
Paslawska, U.
Noszczyk-Nowak, A.
Janiszewski, A.
Gajek, J.
Nicpon, J.
Kiczak, L.
Bania, J.
Zacharski, M.
Tomaszek, A.
Jankowska, E.A.
Ponikowski, P.
Witkiewicz, W.
Powiązania:
https://bibliotekanauki.pl/articles/32380.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Ventricular tachycardia may lead to haemodynamic deterioration and, in the case of long term persistence, is associated with the development of tachycardiomyopathy. The effect of ventricular tachycardia on haemodynamics in individuals with tachycardiomyopathy, but being in sinus rhythm has not been studied. Rapid ventricular pacing is a model of ventricular tachycardia. The aim of this study was to determine the effect of rapid ventricular pacing on blood pressure in healthy animals and those with tachycardiomyopathy. A total of 66 animals were studied: 32 in the control group and 34 in the study group. The results of two groups of examinations were compared: the first performed in healthy animals (133 examinations) and the second performed in animals paced for at least one month (77 examinations). Blood pressure measurements were taken during chronic pacing - 20 min after onset of general anaesthesia, in baseline conditions (20 min after pacing cessation or 20 min after onset of general anaesthesia in healthy animals) and immediately after short-term rapid pacing. In baseline conditions significantly higher systolic and diastolic blood pressure was found in healthy animals than in those with tachycardiomyopathy. During an event of rapid ventricular pacing, a significant decrease in systolic and diastolic blood pressure was found in both groups of animals. In the group of chronically paced animals the blood pressure was lower just after restarting ventricular pacing than during chronic pacing. Cardiovascular adaptation to ventricular tachycardia develops with the length of its duration. Relapse of ventricular tachycardia leads to a blood pressure decrease more pronounced than during chronic ventricular pacing.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spleen content of selected polyphenols, splenocytes morphology and function in mice fed Rhodiola kirilowii extracts during pregnancy and lactation
Autorzy:
Lewicki, S.
Stankiewicz, W.
Skopinska-Rozewska, E.
Wilczak, J.
Lesniak, M.
Suska, M.
Siwicki, A.K.
Skopinski, P.
Zdanowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/31572.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The genus Rhodiola (Crassulaceae) consists of many species, growing mainly in Asia and traditionally used as adaptogens and anti-inflammatory drugs. In order to elaborate herbal immunostimulator which could be safely given to pregnant women, we performed a study on immunotropic effects of feeding pregnant and lactating mice Rhodiola kirilowii extracts. This paper presents the results of the first part of our study – spleen content of selected polyphenols, spleen cellularity, splenocytes phenotype and their response to mitogens. Experiments were performed on adult inbred females of Balb/c strain, mated with adult males. Females, since copulatory plug was noted, up to the 28-th day after delivery were fed daily with 20 mg/kg b.m. water (RKW) or hydro- lcoholic (RKW-A) extracts of Rhodiola kirilowii. Results: 1. Significantly lower proportion of pregnant mice in experimental groups than in the control. 2. Cellularity of spleen and flavonol quercetin spleen concentration were significantly lower in experimental groups in comparison to the controls. 3. Flavanols ( (+)-catechin and epicatechin) levels were significantly higher in the spleens of experimental mice than in the controls. 4. Positive correlation between spleen cellularity and quercetin, and negative correlation between spleen cellularity and epicatechin content were observed. 5. Spleen mass and spleen lymphocytes phenotype and proliferation in RKW and RKW-A fed mice did not differ from the control. These results, together with suspicion of some embryo- oxicity, are worrying and eliminate the possibility of use Rhodiola kirilowii extracts for long-term treatment in pregnant females.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Semen quality assessments and their significance in reproductive technology
Autorzy:
Kordan, W.
Fraser, L.
Wysocki, P.
Strzezek, R.
Lecewicz, M.
Mogielnicka-Brzozowska, M
Dziekonska, A
Soliwoda, D
Koziorowska-Gilun, M
Powiązania:
https://bibliotekanauki.pl/articles/32408.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Semen quality assessment methods are very important in predicting the fertilizing ability of persevered spermatozoa and to improve animal reproductive technology. This review discusses some of the current laboratory methods used for semen quality assessments, with references to their relevance in the evaluation of male fertility and semen preservation technologies. Semen quality assessment methods include sperm motility evaluations, analyzed with the computer-assisted semen analysis (CASA) system, and plasma membrane integrity evaluations using fluorescent stains, such as Hoechst 33258 (H33258), SYBR-14, propidium iodide (PI), ethidium homodimer (EthD) and 6-carboxyfluorescein diacetate (CFDA), and biochemical tests, such as the measurement of malondialdehyde (MDA) level. This review addresses the significance of specific fluorochromes and ATP measurements for the evaluation of the sperm mitochondrial status. Laboratory methods used for the evaluation of chromatin status, DNA integrity, and apoptotic changes in spermatozoa have been discussed. Special emphasis has been focused on the application of proteomic techniques, such as two-dimensional (2-D) gel electrophoresis and liquid chromatography mass spectrometry (LC-MS/MS), for the identification of the properties and functions of seminal plasma proteins in order to define their role in the fertilization-related processes.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Suppressive effect of electromagnetic field on analgesic activity of tramadol in rats
Autorzy:
Bodera, P.
Stankiewicz, W.
Antkowiak, B.
Paluch, M.
Kieliszek, J.
Sobiech, J.
Zdanowski, R.
Wojdas, A.
Siwicki, A.K.
Skopinska-Rozewska, E.
Powiązania:
https://bibliotekanauki.pl/articles/32288.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
suppressive effect
electromagnetic field
analgesic activity
tramadol
rat
analgesic effect
pain perception
Opis:
The electromagnetic fields (EMFs) have been shown to alter animal and human behavior, such as directional orientation, learning, pain perception (nociception or analgesia) and anxiety-related behaviors. The aim of this study was to evaluate the influence of electromagnetic fields of high-frequency microwaves on pain perception and anti-nociceptive activity of tramadol (TRAM) - analgetic effective in the treatment of moderate to severe acute and chronic pain states. Electromagnetic fields exposures of a) 1500 MHz frequency and b) modulated, 1800 MHz (which is identical to that generated by mobile phones) were applied. Paw withdrawal latency (PWL) to thermal stimulus was measured in vehicle or tramadol (TRAM) treated animals before and after 30, 60 and 90 minutes from injections. The differences in the level of pain (PWL) between control group and rats exposed to EMF alone in three measurements, were not observed. Tramadol alone significantly increased PWLs to thermal stimulus in comparison to vehicle results at 30 (p < 0.001) and 60 minutes (p < 0.05) after drug injection. EMF exposure of both frequencies transiently suppressed analgesic effect of tramadol, significantly reducing paw withdrawal latency in animals treated with this drug at 30 minutes from the drug injection.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Concentration levels of cadmium and lead in the raw and processed meat of Helix pomatia snails
Autorzy:
Ziomek, M.
Drozd, Ł.
Chałabis-Mazurek, A.
Szkucik, K.
Paszkiewicz, W.
Valverde Piedra, J. L.
Bełkot, Z.
Maćkowiak-Dryka, M.
Gondek, M.
Knysz, P.
Powiązania:
https://bibliotekanauki.pl/articles/2087661.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cadmium
lead
GFAAS
snail meat
Helix pomatia
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 483-489
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a blocking immunoperoxidase monolayer assay for differentiation between pseudorabies virus-infected and vaccinated animals
Autorzy:
Wang, Y.B.
Li, Y.H.
Li, Q.M.
Xie, W.T.
Guo, C.L.
Guo, J.Q.
Deng, R.G.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/2087396.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pseudorabies virus variant strains
anti-pseudorabies virus monoclonal antibody
blocking immunoperoxidase monolayer assay
differentiation between pseudorabies virus-infected and vaccinated animals
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 717-723
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of probiotic and yeast culture supplementation on selected biochemical and immunological parameters of growing lambs
Autorzy:
Mahmoud, M.M.
Youssef, I.M.I.
Abd El-Tawab, M.M.
Bakr, H.A.
Eissa, N.A.
Hassan, M.S.
Giadinis, N.D.
Milewski, S.
Baumgartner, W.
Sobiech, P.
Powiązania:
https://bibliotekanauki.pl/articles/2087359.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
lambs
probiotic
yeast culture
Saccharomyces cerevisiae
immunoglobulins
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 5-12
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
17 beta-estradiol affects proliferation and apoptosis of canine bone marrow mesenchymal stem cells in vitro
Autorzy:
Zhou, Z.-H.
Gu, C.-W.
Li, J.
Huang, X.-Y.
Deng, J.-Q.
Shen, L.-H.
Cao, S.-Z.
Deng, J.-L.
Zuo, Z.-C.
Wang, Y.
Ma, X.-P.
Ren, Z.-H.
Yu, S.-M.
Powiązania:
https://bibliotekanauki.pl/articles/2087308.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
apoptosis
bone marrow mesenchymal stem cells (BMSCs)
canine
proliferation
17 beta-estradiol (E2)
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 235-245
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
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