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Tytuł:
Feeding milk replacer instead of whole milk affects blood plasma proteome and lipid profile in preruminant calves
Autorzy:
Lepczynski, A.
Herosimczyk, A.
Ozgo, M.
Skrzypczak, W.F.
Powiązania:
https://bibliotekanauki.pl/articles/2087979.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The study was undertaken to determine the effect of feeding milk or milk-replacer on the blood plasma proteome and lipid profile in calves during the second week of life. Feeding milk-replacer significantly decreased the expression of plasma apoA-I. Age of calves affected apoA-I expression, which was higher on the 8th than on the 11th and 14th day of life. A significant effect of interaction between diet and age was also observed. The expression of apoA-IV, was significantly affected by diet and was lower in calves fed milk replacer. Expression of this protein was significantly lower at the 8th day of life and was up-regulated in the calves fed milk-replacer at the second week of life. Calves fed milk-replacer had greater expression of haptoglobin, which differed significantly between days of blood sampling, being higher on the 8th than on the11th and 14th day. The interactive effect of diet and age affected haptoglobin expression, which was successively down-regulated in calves fed milk replacer. Diet had a significant effect on the plasma lipid profile. Animals fed milk had a greater concentration of TC, HDLC and LDLC. The composition of milk-replacer, especially fat source, is probably the main factor that affects expression of proteins involved in cholesterol metabolism and level of components of lipid profile in calves fed formula. We claim that the initially increased level of haptoglobin, followed by its decrease during the second week of life in calves fed milk-replacer may indicate the presence of short-term stress induced by changes in the feeding system.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in lipid metabolism during last month of pregnancy and first two months of lactation in primiparous cows-analysis of apolipoprotein expression pattern and changes in concentration of total cholesterol, HDL, LDL, triglycerides
Autorzy:
Kurpinska, A.K.
Jarosz, A.
Ozgo, M.
Skrzypczak, W.F.
Powiązania:
https://bibliotekanauki.pl/articles/30783.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The final weeks of pregnancy and period of increasing lactation abound with adaptive changes in the intensity of metabolic processes. Maintaining the homeostasis of an organism in prepartum and postpartum periods is the key condition in maintaining the health of the mother and the fetus/calf. The aim of the study was to analyze physiological changes in lipid metabolism in cows during the last month of first pregnancy and in the first two months of lactation, based on the expression of identified apolipoproteins and changes in selected parameters of the lipid metabolism in peripheral blood plasma. Statistically significant changes in the expression of identified apolipoproteins were observed for apolipoprotein A-1 precursor, apolipoprotein A-IV precursor, apolipoprotein E precursor and apolipoprotein J precursor. The lowest expression of the apolipoproteins was noted around parturition and higher expression was observed during the final weeks of pregnancy and during lactation. Tendencies of changes in the concentration of total cholesterol, HDL and LDL were similar in blood plasma from analyzed cows - in the last month of pregnancy a decrease was observed and subsequently an increase in the first two months of lactation was noted. In contrast to abrupt changes observed for total cholesterol, HDL and LDL, changes in concentration of triglycerides were not that extensive and during lactation this parameter was rather stable. Evaluation of changes in the analyzed parameters may contribute to a better understanding of the changes in lipid metabolism occurring in the body of pregnant and lactating young cows.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Blood plasma protein and lipid profile changes in calves during the first week of life
Autorzy:
Herosimczyk, A.
Lepczynski, A.
Ozgo, M.
Dratwa-Chalupnik, A.
Michalek, K.
Skrzypczak, W.F.
Powiązania:
https://bibliotekanauki.pl/articles/30987.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The present study was undertaken to determine blood plasma protein and lipid profile changes in healthy Polish Holstein-Fresian calves of Black-and-White variety. Blood was drawn immediately after birth, before first colostrum intake and at the 3rd, 6th, 12th, 24th, 36th, 48th and 72nd hour of life. Subsequent four blood samples were collected at 24 hour intervals until the 7th day of life. Plasma proteins within the isoelectric point ranging from 3.0 to 10.0 were separated using high resolution two-dimensional electrophoresis. Among the 74 protein spots detected and analyzed, 16 were significantly altered during the first week of life. Differentially expressed spots were excised from the gels and subjected to peptide mass fingerprinting using MALDI-TOF MS. In total, 12 spots were successfully identified, which correspond to three proteins, namely: apolipoprotein A-I, apolipoprotein A-IV and fibrinogen gamma-B chain. A gradual increase in plasma triglyceride, total cholesterol, HDL and LDL cholesterol values was shown during the first seven days of calves life. The lowest concentration of these indicators were observed at birth and was followed by a rapid increase during the first week of postnatal life. These changes appear to be related to the transition in energy sources, from a maternal nutrient supply comprising mainly carbohydrates and amino acids to a diet which was rich in fat – colostrum and milk. This was reflected by the intense up-regulation of plasma proteins related with lipid transport and lipoprotein metabolism during the first week of life.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mapping of polar fox renal cortex proteins using two-dimensional gel electrophoresis and mass spectrometry – a preliminary study
Autorzy:
Ciechanowicz, A.K.
Ozgo, M.
Stanski, L.R.
Herosimczyk, A.
Piotrowska, A.
Szymeczko, R.
Laszczynska, M.
Skrzypczak, W.F.
Powiązania:
https://bibliotekanauki.pl/articles/30461.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the present study was to establish protein map of polar fox (Alopex lagopus) renal cortex. Kidney cortex proteins of isoelectric point ranging from 3 to 10 were analysed using two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Sixteen protein spots corresponding to thirteen different gene products were identified. These proteins were divided into following groups: lipid and fatty acid metabolism, amino acid metabolism, energetic pathways, regulatory proteins, transport proteins and structural proteins. This is the first attempt to create reproducible 2-D map, of renal cortex proteins characteristic for polar foxes, used as animal model for carnivores. It is worth emphasizing that the results of this study may broaden currently available protein databases.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 231-237
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and sequence analysis of the complete VP2 gene of canine parvovirus from Chinese domestic pets and determination of the pathogenesis of these circulating strains in beagles
Autorzy:
Chen, M.R.
Guo, X.Y.
Wang, Z.Y.
Jiang, Y.T.
Yuan, W.F.
Xin, T.
Hou, S.H.
Song, T.Q.
Lin, W.D.
Zhu, H.F.
Jia, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087542.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
canine parvovirus
molecular epidemiology
phylogenetic analysis
pathogenesis
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 287-296
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protein expression changes in cells inoculated with Equine Influenza Virus: antibody microarray analysis
Autorzy:
Rozek, W.
Kwasnik, M.
Zmudzinski, J.F.
Powiązania:
https://bibliotekanauki.pl/articles/30270.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Changes in the level of cellular proteins in cells inoculated with equine influenza virus H7N7 and H3N8 were studied with microarray technique. H3N8 induced pro-apoptotic proteins while H7N7 induced both pro- as well as anti-apoptotic factors. The higher level of some cytoskeleton components and proteins involved in the protein quality control was recorded. Relatively high number of proteins involved in the regulation of transcription was down-regulated. The pattern of changes observed for H7N7 and H3N8 may reflect differences in the biological properties of both serotypes.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Variability of non-structural proteins of equine arteritis virus during persistent infection of the stallion
Autorzy:
Socha, W.
Rola, J.
Zmudzinski, J.F.
Powiązania:
https://bibliotekanauki.pl/articles/31412.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The genetic stability of ORF1a encoding non-structural proteins nsp1, nsp2, nsp3 and nsp4 of equine arteritis virus (EAV) has been analysed for nearly seven years in a persistently infected stallion of the Malopolska breed. Between November 2004 and June 2011, 11 semen samples were collected. Viral RNA extracted from semen of this carrier stallion was amplified, sequenced and compared with the sequences of the other known strains of EAV. Sequence analysis of ORF1a showed 84 synonymous and 16 non-synonymous mutations. The most variable part of ORF1a was the region encoding nsp2 protein with 13 non-synonymous substitutions. The degree of amino acid identity between isolates ranged from 98.91 to 100%. Only single non-synonymous mutations were detected in nsp1 (one substitution) and nsp4 (two substitutions). The most stable was nsp3 in which no amino acid substitutions were observed during the whole period of observation.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influenza virus proteins as factors involved in interspecies transmission
Autorzy:
Gora, I.M.
Rozek, W.
Zmudzinski, J.F.
Powiązania:
https://bibliotekanauki.pl/articles/32083.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Influenza A viruses cause recurrent epidemics and global pandemics. One of the unique features of influenza virus is the ability to overcome interspecies barrier. Reassortment of viral genes and the accumulation of mutations contribute to the emergence of new influenza virus variants. The replication of influenza A virus in a specific host depends on many factors e.g. activity of viral proteins, host response system and environmental conditions. In this review the role of viral proteins as a condition for crossing the species barriers is discussed.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protective effect of lithium chloride on pulmonary injury caused by Actinobacillus pleuropneumoniae via inhibition of GSK-3β-NF-κB-dependent pathway
Autorzy:
Zhang, Y.
Xu, W.
Tang, Y.
Huang, F.
Powiązania:
https://bibliotekanauki.pl/articles/16539075.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine contagious pleuropneumonia
mouse model
lithium chloride
toll-likereceptor 4
GSK-3β-NF-κB-dependent pathways
Opis:
Porcine contagious pleuropneumonia (PCP) is a very serious respiratory disease which is difficult to prevent and treat. In this study, the therapeutic effects of lithium chloride (LiCl) on PCP were examined using a mouse model. A mouse model of PCP was established by intranasal infections with Actinobacillus pleuropneumoniae (App). Histopathological analysis was performed by routine paraffin sections and an H-E staining method. The inflammatory factors, TLR4 and CCL2 were analyzed by qPCR. The expression levels of p-p65 and pGSK-3ß were detected using the Western Blot Method. The death rates, clinical symptoms, lung injuries, and levels of TLR-4, IL-1ß, IL-6, TNF-α, and CCL2 were observed to decrease in the App-infected mice treated with LiCl. It was determined that the LiCl treatments had significantly reduced the mortality of the App-infected cells, as well as the expressions of p-p65 and pGSK-3ß. The results of this study indicated that LiCl could improve the pulmonary injuries of mice caused by App via the inhibition of the GSK-3β-NF-κB-dependent pathways, and may potentially become an effective drug for improving pulmonary injuries caused by PCP.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 35-44
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The study on bactericidal effect and ultrastructural alterations of chlorocresol nanoemulsion disinfectant against Staphylococcus aureus
Autorzy:
Zhang, Y.F.
Sun, Y.W.
Liu, X.H.
An, Z.X.
Yang, X.F.
Powiązania:
https://bibliotekanauki.pl/articles/16647452.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
chlorocresol
nanoemulsion
disinfectant
Staphylococcus aureus
bactericidal effect
ultrastructure
Opis:
Chlorocresol nanoemulsion disinfectant (CND) is an environmental disinfectant prepared with nanoemulsion as its drug carrier. This study aimed to investigate the bactericidal effect of CND on Staphylococcus aureus ( S. aureus) and its effect on bacterial ultrastructure. The neutralizing effect of CND against S. aureus was first screened by suspension quantitative evaluation experiment procedure of neutralizer. Disinfection performance was evaluated by the determination of Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), quantitative bactericidal experiment, and comparative experiment of disinfection performance between 0.1% CND and 0.1% chlorocresol aqueous solution. Meanwhile, the effect of CND on the ultrastructure of S. aureus was investigated with scanning electron microscope (SEM) and transmission electron microscope (TEM) to preliminarily explore the bactericidal mechanism. The results showed that 3% Tween-80 in PBS could be screened as the neutralizer of CND against S. aureus. MIC and MBC were 100 μg/mL and 200 μg/mL, respectively. The bactericidal rates were all 100% when 0.06% and 0.08% disinfectant acted for 15 and 5 min, respectively. Furthermore, compared with 0.1% chlorocresol aqueous solution, the bactericidal effect of 0.1% CND was significantly enhanced (p<0.01). After treatment with CND for 10 min, SEM observation showed that the morphology of S. aureus cells were changed and the integrity destroyed. TEM observation showed that the cell shape changed, and the structures of the cell wall, cell membrane and cytoplasm were damaged in varying degrees. CND showed the strong bactericidal effect on S. aureus and could cause ultrastructure alterations of S. aureus.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 13-20
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of matrine on reducing damage to bovine mammary epithelial cells induced by Staphylococcus aureus alpha-hemolysin
Autorzy:
Feng, F.
Ma, W.W.
Luo, H.X.
Guan, C.P.
Zhou, X.Z.
Powiązania:
https://bibliotekanauki.pl/articles/2087720.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
α-hemolysin
BMECs
matrine
cell damage
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 409-413
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of loop-mediated isothermal amplification (LAMP) assays for the detection of bovine herpesvirus 1
Autorzy:
Socha, W.
Rola, J.
Urban-Chmiel, R.
Żmudziński, J.F.
Powiązania:
https://bibliotekanauki.pl/articles/2087889.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Polish Journal of Veterinary Sciences; 2017, 3; 619-622
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence, associated risk factors and clinico-pathological studies of buffalopox disease in various regions of Punjab province, Pakistan
Autorzy:
Usmani, M.W.
Rizvi, F.
Khatoon, A.
Mahmood, M.S.
Powiązania:
https://bibliotekanauki.pl/articles/16539103.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
seropositive
single radial hemolysis
scab samples
PCR
C18L gene
leukocytes
Opis:
Buffalopox (BPX) is a highly contagious disease that causes high morbidity and production losses in buffaloes. During this study, seroprevalence, effect of various associated risk factors, and pathological studies of BPX were recorded in the Punjab province. A total of 97 blood samples and 63 scabs were collected from clinically pox suspected buffaloes. Serum was harvested to perform single radial hemolysis to assess the seroprevalence, and scabs were subjected to PCR for BPX virus confirmation. Results revealed that, animal demographics and environmental associated factors showed significant effect (p<0.05,1<R2>0) on BPX occurrence. The overall BPX seroprevalence was recorded 4.18% in the Punjab province. The BPX was recorded 5.48% in Nili Ravi breed during winter (7.42%), aged 5-7 years (7.46%) under loose housing (5.51%) in the Faisalabad region (8.03%). Further, BPX was 5.37% in pregnant, 6.86% pregnant milking buffaloes during the 3rd lactation period (7.28%) in dairy herds (5.20%). The BPX was 5.22% in non-vaccinated buffaloes where multiple animals were reared together (4.99%) in the herds having 21-30 total number of animals. A total of 49 scab samples were found positive for the BPX virus via PCR with C18L gene amplification. Grossly, inflammatory lesions with pits in the center and wart-like nodules were seen on teats and udder of buffaloes. Increased leukocytes, especially neutrophils and lymphocytes, were seen in the blood of the infected animals. These results provide a broader window to understand the effect of associated risk factors, strengthen the diagnostic aid, and to contain the current spread of BPX in Pakistan to safeguard large ruminant-based livelihood.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 137-147
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of porcine epidemic diarrhea virus infection on Toll-like receptor expression and cytokine levels in porcine intestinal epithelial cells
Autorzy:
Wang, F.
Wang, S.Q.
Wang, H.F.
Wu, Z.C.
Bao, W.B.
Wu, S.L.
Powiązania:
https://bibliotekanauki.pl/articles/2087351.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pig
porcine epidemic diarrhea virus
TLRs
innate immunity
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 119-126
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An effect of mammary gland infection caused by Streptococcus uberis on composition and physicochemical changes of cows’ milk
Autorzy:
Pecka-Kielb, E.
Vasil, M.
Zachwieja, A.
Zawadzki, W.
Elecko, J.
Zigo, F.
Illek, J.
Farkasova, Z.
Powiązania:
https://bibliotekanauki.pl/articles/31243.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
An effect of mammary gland infection caused by Streptococcus uberis on the changes in cows’ milk composition and its physicochemical properties was examined. The study was conducted in the herd of Slovak Pied breed cattle (with a share of HF blood), in 2nd and 3rd lactation, after 4th month of milking. Milk samples were collected from a quarter milking. The samples were subjected to microbiological analysis, basic milk composition, total bacteria count, somatic cell count and physicochemical properties were examined. Also analyses of protein fractions share and fatty acids profile were conducted. An effect of bacterial infection of the mammary gland bring an increase (P<0.01) in somatic cell count was observed in this study. Milk samples contaminated with S. uberis were characterized by higher (P<0.05) total bacteria count and total protein compared to milk samples collected from non-infected mammary gland. The level of κ-casein was significantly (P<0.05) decreased in cows with subclinical mastitis caused by S. uberis. Significant (P<0.05) reduction in the share of C13:0 acid, and an increased level of C18:0, C18:1n7t and CLA were observed in milk contaminated with S. uberis compared to healthy cows’ milk. It should be concluded that S. uberis causes the increase in total bacteria count, SCC and the decrease in κ-casein level, which significantly affects deterioration of technological quality of cows’ milk.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł

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