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Wyświetlanie 1-15 z 143
Tytuł:
Pituitary adenylate cyclase-activating peptide-27 (PACAP-27) is co-stored with galanin, substance P and corticotropin releasing factor (CRF) in intrapancreatic ganglia of the sheep
Autorzy:
Arciszewski, M.B.
Mozel, S.
Sienkiewicz, W.
Powiązania:
https://bibliotekanauki.pl/articles/31633.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide existing in two variant forms (of either 27 or 38 residues), widely present in numerous organs and evoking multiple effects both in the central and peripheral nervous systems. The present study was undertaken to evaluate the distribution pattern of PACAP-27 expression in the ovine pancreas. Using double immunohistochemical stainings co-localizations of PACAP-27 with galanin, SP or CRF were studied in intrapancreatic neurons. In intrapancreatic ganglia, immunoreactivty to PACAP-27 was found in 87.6 ± 5.4% of PGP 9.5-positive intrapancreatic neurons but not in intraganglionic nerve fibres. Numerous PACAP-27-im-munoreactive nerve terminals were also observed between pancreatic acini and around small arterioles. No immunoreactivity to PACAP-27 was found in the endocrine pancreas. In 42.9 ± 6.2% of PACAP-27-immunoreactive intrapancreatic neurons the expression of galanin was also found. Statistically lower subpopulation (12.4 ± 4.0%) of intrapancreatic neurons exhibited simultaneously the immunoreactivity to PACAP-27 and SP. The expression of CRF was detected in the relatively smallest group (3.2 ± 1.4%) of PACAP-27-positive intrapancreatic neurons. The present results suggest that in the ovine pancreas PACAP-27 may play an important role as mediator of pancreatic functions. In PACAP-related pancreatic activities, a modulatory role of galanin, SP and to a lower extend of CRF is also likely.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunocytochemical detection of calretinin in the claustrum and endopiriform nucleus of the chinchilla
Autorzy:
Szalak, R.
Matysek, M.
Mozel, S.
Arciszewsk, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/30835.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Calretinin (CR) as a buffer and sensor protein plays an important role in regulatory processes of Ca²⁺ and anty-apoptotic cellular protection. In the present study, immunohistochemical peroxidase- antiperoxidase (PAP) method was used in order to determine the numbers, morphology, morphometry and distribution pattern of CR in neurons of the chinchilla’s claustrum (Cl) and endopiriform nucleus (EN). In Cl and EN the presence of several classes of neurons with different immunoreactivity to CR was found. In Cl, CR-immunoreactive (IR) neurons were predominantly found in close vicinity to insular border while CR-IR neurons were evenly scattered throughout EN. In general, immunoreaction to CR was observed in neuronal cytoplasm, nucleus and in fibres-like nerve extensions. Statistical analysis showed the differences between average large diameter as well as cross-sectional area of CR-IR neurons present in Cl and EN. It is suggested, that CR acting as a calcium binding protein may play a role in neuronal network. Further co-localization studies are necessary to fully elucidate neurophysiology and neuropathology of the chinchilla’s Cl and EN neurons.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunoreactivity of acetylcholinesterase and M1 muscarinic receptors in the hippocampus and striatum of rats treated with Rebaudioside A
Autorzy:
Rycerz, K.
Jaworska-Adamu, J.
Krawczyk, A.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/16539186.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
rebaudioside A
stevia
acetylcholinesterase
hippocampus
striatum
rat
Opis:
Rebaudioside A (RebA) is a steviol glycoside used for production of sweeteners. It was shown that the glycosides affect memory and learning processes. The aim of the study was to investigate neurons immunoreactive for acetylcholinesterase (AChE) and M1 muscarinic receptors (mAChRs-M1) of the hippocampal CA1 and CA3 fields and striatal caudateputamen (CP) and globus pallidus (GP) in rats receiving RebA. RebA was administrated to adult rats for 45 days in dilutions of 1 mg and 2 mg RebA/ml water. Indirect peroxidase-antiperoxidase immunohistochemical reaction was conducted on frontal sections containing the hippocampus and striatum with use of antibodies against AChE and mAChRs-M1. Immunoreactive for the studied proteins neurons were morphologically and morphometrically assessed in hippocampal CA1 and CA3 fields and in CP and GP. Microscopic observations did not reveal significant changes in morphology of immunoreactive neurons, which suggests no neurotoxic effect of the studied glycoside on these cells. Morphometric analyses revealed an increase in the density of AChE and mAChRs-M1 immunoreactive neurons. A decrease in reaction intensity of AChE-positive neurons was also demonstrated in the hippocampal CA1 field and in GP. In contrast, an increase in reaction intensity of mAChRs-M1-positive neurons was found in CA1, CA3 fields and in CP and GP. The results of our preliminary studies indicate that RebA administrated to rats has an impact on cholinergic neurons in the studied area. The results suggest a possible increase in the activity of the cholinergic system, responsible for memory and learning processes, after administration of RebA.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 303-310
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in expression of calbindin 28 kDa in the small intestine of red kidney bean (Phaseolus vulgaris) lectin-treated suckling piglets
Autorzy:
Zacharko-Siembida, A.
Valverde Piedra, J.L.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/30868.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Calbindin (CB) is a calcium binding protein playing a role in calcium uptake and anti-apoptotic cellular protection. The expression of CB was immunohistochemically studied in the small intestine of normal and red bean kidney lectin-treated suckling piglets. In the duodenum and jejunum (but not ileum) of lectin-treated animals overexpression of CB was noted in chromogranin A-immunoreactive (CgA-IR) neuroendocrine (NE) cells. In both control and experimental group a small population of CB-IR NE cells exhibited the presence of somatostatin (but not serotonin, histamine or CRF). After the lectin treatment, an increased (however not statistically significant) immunoreactivity to CB was found in a small subpopulation of neurons of outer submucous (but not inner submucous and myenteric) plexus. It is suggested that there is a functional interaction between lectin administration and CB-expression in the porcine small intestine. Future studies will be needed to clarify this processes.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of obesity on the bone morphometry and histomorphometry in male and female Wistar rats
Autorzy:
Wolski, D.
Wawrzyniak, A.
Bieńko, M.
Michalik, J.
Radzki, R.P.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/2087700.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bone tissue
histomorphometry
humerus
obesity
osteocytes
adipocytes
rats
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 599-608
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical study on the expression of biologically active substances in the endocrine pancreas of the European bison
Autorzy:
Mozel, S.
Szymańczyk, S.
Krzysiak, M.
Puzio, I.
Zacharko-Siembida, A.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/2087293.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
glucagon-like peptide-1
glucagon
insulin
somatostatin
immunohistochemistry
pancreas
European bison
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 333-340
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of feeding with bilberry fruit on the expression pattern of alphaCaMKII in hippocampal neurons in normal and diabetic rats
Autorzy:
Matysek, M.
Mozel, S.
Szalak, R.
Zacharko-Siembida, A.
Obszanska, K.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/30281.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
αCaMKII, widely occurring in the central nervous system, plays a significant role in cognitive processes. It is well known that diabetes is a risk factor that may trigger brain atrophy, cognitive dysfunction and finally lead to memory loss. Antioxidants richly present in bilberry fruits are believed to have significant effects on diabetes-related brain dysfunctions mainly due to their abilities to modulate neurotransmitter release that lead to reduction of the negative impact of free radicals on cognitive processes. The aim of the present research was to immunohistochemically investigate the expression patterns of αCaMKII in hippocampal neurons from non-diabetic, diabetic and diabetic rats fed with an extract of bilberry fruit. The obtained results show that in comparison to the control group, in diabetic rats hippocampal neurons immunoreactive (ir) to αCaMKII were swollen and the lengths of the neuronal fibres were reduced. Further study shows that in diabetic rats fed with bilberry fruit, αCaMKII-positive nerve fibres were significantly longer when compared to the groups of diabetic and control rats. Additionally, we observed statistically significant changes in the average larger diameter of αCaMKII-ir hippocampal neurons between groups of diabetic rats (with vs. Without supplement of bilberry fruit). The results of the present work suggest that antioxidants present in bilberry fruits influence the morphology of and possibly exhibit beneficial and neuroprotective effects on hippocampal neurons during diabetes. It is likely that changes in the appearance of αCaMKII-expressed hippocampal neurons may reflect the diabetes-evoked rise in Ca²⁺ level in the cerebral nerve terminals. The present research extends our knowledge of preventive mechanisms for cognitive dysfunctions occurring in the brain during diabetes.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig
Autorzy:
Zacharko-Siembida, A.
Matysek, M.
Szalak, R.
Radlinska, A.
Obszanska, K.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/31220.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Cocaine- and amphetamine-regulated transcript (CART) peptides are widely expressed not only in the brain but also in numerous endocrine/neuroendocrine cells as well as in neurons of the peripheral nervous system. The present study investigated the distribution patterns of CART-like immunoreactivity in the pelvic plexus (PP) of the female pig. The co-expression of CART with principal neurotransmitter markers: choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), serotonin (5-HT) or biologically active neuropeptides: pituitary adenylate cyclase-activating polypeptide (PACAP), substance P (SP), calbindin was analyzed using double immunohistochemical stainings. Amongst neurons immunopositive to Hu C/D panneuronal marker as many as 4.1 ± 1.2% in right and 4.4 ± 1.6% in left pelvic ganglia were found to express CART. The vast majority of CART-IR ganglionic neurons were predominantly small in size and were evenly scattered throughout particular ganglia. Immunoreactivity to CART was also detected in numerous nerve terminals (which frequently formed pericellular formations around CART-negative perikarya) as well as in numerous nerve fibres within nerve branches interconnecting the unilateral pelvic ganglia. Immunohistochemistry revealed that virtually all CART-IR neurons were cholinergic in nature and CART-IR basket-like formations frequently encircled TH-positive/CART-negative perikarya. None of CART-IR ganglionic neurons showed immunoreactivity to SP, PACAP, 5-HT or calbindin. CART-IR nerve fibres ran in a close vicinity to serotonin-containing cells or faintly labelled SP-expressing neurons. On the other hand, PACAP-IR, SP-IR (but not 5-HT-positive) nerve terminals were found to run in close proximity to CART-IR neurons. Our results indicate that: 1) CART present in PP may influence the activity of pelvic ganglionic neurons/SIF cells, 2) PP should be considered as a potential source of CART-like supply to pelvic viscera and 3) functional interactions between CART and SP or PACAP are possible at the periphery.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Colocalization pattern of cocaine- and amphetamine-regulated transcript peptide and parvalbumin immunoreactivity in the hippocampus proper of the chinchilla
Autorzy:
Szalak, R.
Matysek, M.
Kukula-Koch, W.
Rycerz, K.
Zacharko-Siembida, A.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/2087337.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
hippocampus
dentate gyrus
CART
PV
Ca2+
memory
chinchilla
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 89-96
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of biochemical and protein profiles of seminal plasma to prediction of semen quality and fertility in stallions
Autorzy:
Stelletta, C.
Alberti, S.
Cil, B.
Tekin, K.
Tirpan, M.B.
Arganaraz, M.
Akcay, E.
Daskin, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087155.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fertility
seminal plasma biochemistry
seminal plasma proteins
stallion
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 505-514
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of bilberry fruit on memory and the expression of parvalbumin in the rat hippocampus
Autorzy:
Borowiec, K.
Matysek, M.
Szwajgier, D.
Biała, G.
Kruk-Słomka, M.
Szalak, R.
Ziętek, J.
Arciszewski, M.B.
Targoński, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2087496.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bilberry
calcium-binding proteins
hippocampus
memory
rat
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 481-487
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Histomorphometrical changes in intestine structure and innervation following experimental fumonisins intoxication in male Wistar rats
Autorzy:
Rudyk, H.
Tomaszewska, E.
Arciszewski, M.B.
Muszyński, S.
Tomczyk-Warunek, A.
Dobrowolski, P.
Donaldson, J.
Brezvyn, O.
Kotsyumbas, I.
Powiązania:
https://bibliotekanauki.pl/articles/2087355.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mycotoxins
food contamination
small intestine morphology
rodents
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 77-88
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adrenergic control of pinealocyte chondriome - an in vitro study
Autorzy:
Przybylska-Gomowicz, B.
Lewczuk, B.
Ziolkowska, N.
Prusik, M.
Powiązania:
https://bibliotekanauki.pl/articles/31742.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Norepinephrine released from sympathetic innervation plays the main role in the regulation of melatonin secretion in mammalian pinealocytes. The present study was conducted for the following reasons: 1) to establish whether the pinealocyte chondriome is controlled by norepinephrine, 2) to determine the effect of adrenergic stimulation on mitochondria, and 3) to characterize adrenoceptors involved in the regulation of the chondriome. The static organ culture of the pineal gland was used. The explants were incubated for 5 consecutive days in control medium and between 20:00 and 08:00 in medium with the presence of 10 μM norepinephrine – adrenergic agonist; isoproterenol – beta-adrenoceptor agonist; cirazoline, methoxamine, M-6364 – alfa1 – adrenoceptors agonists or PMA – activator of PKC. The explants were then subjected to ultrastructural examination and morphometric analysis. The incubation of explants in the presence of norepinephrine or isoproterenol caused a decrease in the relative volume and the numerical density of mitochondria and induced an increase in the percentage of free mitochondria in pinealocytes. Significant changes in these parameters were not observed after treatment with methoxamine, cirazoline, M-6463 and PMA. The results obtained show that the chondriome of pig pinealocytes is controlled by norepinephrine acting via beta-adrenoceptors. Adrenergic stimulation, repeated for five consecutive days of organ culture, causes a decrease in the number of mitochondria and a shift in the distribution of mitochondria from the form of networks and filaments into the form of single particles. This indicates the intensive remodeling of the mitochondria network, which is closely linked to the metabolic status of the cell.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Regulation of melatonin secretion in the pineal organ of the domestic duck - an in vitro study
Autorzy:
Prusik, M.
Lewczuk, B.
Ziolkowska, N.
Przybylska-Gornowicz, B.
Powiązania:
https://bibliotekanauki.pl/articles/32505.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of study was to determine the mechanisms regulating melatonin secretion in the pineal organs of 1-day-old and 9-month-old domestic ducks. The pineals were cultured in a superfusion system under different light conditions. Additionally, some explants were treated with norepinephrine. The pineal glands of 1-day-old ducks released melatonin in a well-entrained, regular rhythm during incubation under a 12 hrs light : 12 hrs dark cycle and adjusted their secretory activity to a reversed 12 hrs dark : 12 hrs light cycle within 2 days. In contrast, the diurnal changes in melatonin secretion from the pineals of 9-month-old ducks were largely irregular and the adaptation to a reversed cycle lasted 3 days. The pineal organs of nestling and adult ducks incubated in a continuous light or darkness secreted melatonin in a circadian rhythm. The treatment with norepinephrine during photophases of a light-dark cycle resulted in: 1) a precise adjustment of melatonin secretion rhythm to the presence of this catecholamine in the culture medium, 2) a very high amplitude of the rhythm, 3) a rapid adaptation of the pineal secretory activity to a reversed light-dark cycle. The effects of norepinephrine were similar in the pineal organs of nestlings and adults. In conclusion, melatonin secretion in the duck pineal organ is controlled by three main mechanisms: the direct photoreception, the endogenous generator and the noradrenergic transmission. The efficiency of intra-pineal, photosensitivity-based regulatory mechanism is markedly lower in adult than in nestling individuals.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The low doses effect of experimental zearalenone (ZEN) intoxication on the presence of Ca2+ in selected ovarian cells from pre-pubertal bitches
Autorzy:
Gajecka, M.
Przybylska-Gornowicz, B.
Powiązania:
https://bibliotekanauki.pl/articles/31848.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of this study was to determine the effect of 42-day ZEN intoxication on the presence of Ca2+ in selected ovarian cells from beagle bitches, using the potassium pyroantimonate (PPA) method. Samples were collected from 30 clinically healthy, pre-pubertal, genetically homogeneous animals. The bitches were divided into three groups of 10 animals each: experimental group I - 50 μg ZEN/kg BW (100% NOAEL) administered once daily per os; experimental group II - 75 μg ZEN/kg BW (150% NOAEL) administered once daily per os; control group - placebo containing no ZEN administered per os. An electron microscopic analysis revealed that cells died due to apoptosis, depending on the ZEN dose and the type of cells exposed to intoxication. Lower ZEN doses led to apoptosis-like changes in the cells. Cell death was a consequence of excess Ca2+ accumulation in the mitochondria, followed by cell dysfunction and a decrease in or the absence of mitochondrial metabolic activity in oocytes, follicle cells and interstitial cells in experimental bitches.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 143

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