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Wyszukujesz frazę "Liu, G." wg kryterium: Autor

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    Wyświetlanie 1-11 z 11
    Tytuł:
    Emerging of canine kobuvirus in dogsin China, 2015
    Autorzy:
    Liu, D.F.
    Liu, X.
    Li, Z.J.
    F. Liu
    Hu, X.L.
    Li, Z.G.
    Liu, C.G.
    Ma, J.Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087863.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    canine kobuvirus (CaKoV)
    dogs
    phylogenetic
    VP1
    China
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 4; 707-711
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China
    Autorzy:
    Li, R.-f.
    Tian, X.-g.
    Liu, Y.
    Xu, J.
    Liu, D.-y.
    Powiązania:
    https://bibliotekanauki.pl/articles/30492.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Porcine insulin receptor substrate 2: molecular cloning, tissues distribution, and functions in hepatocyte and aortic endothelial cells
    Autorzy:
    Yin, Z.
    Cai, M.
    Weng, X.
    Liu, Z.
    Zhang, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087490.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    IRS-2
    molecular cloning
    hepatocytes
    aortic endothelial cells
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 3; 589-598
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of pathogenicity island ETT2 in Escherichia coli isolated from piglets with diarrhea in northeast of China
    Autorzy:
    Yuan, C.W.
    Liu, W.X.
    Hou, J.L.
    Zhang, L.G.
    Wang, G.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087783.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Escherichia coli
    pathogenicity island
    ETT2
    piglets
    diarrhea
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 5-12
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
    Autorzy:
    Dai, G.
    Huang, X.
    Liu, Q.
    Li, Y.
    Zhang, L.
    Han, K.
    Yang, J.
    Liu, Y.
    Xue, F.
    Zhao, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647377.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    epitope
    goose astrovirus
    capsid protein
    monoclonal antibody
    Opis:
    Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig
    Autorzy:
    Hua, Z.
    Xu, G.
    Liu, X.
    Bi, Y.
    Xiao, H.
    Hua, W.
    Li, L.
    Zhang, L.
    Ren, H.
    Zheng, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/31224.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for both agricultural and biomedical research. However, its efficiency is crucially dependent on the source of nuclear donor cells. In this study, we compared the cloning efficiency by using three lines of donor cells that are derived from fetal, newborn and adult fibroblasts of Chinese indigenous Meishan pig. We showed that cleavage rate and blastocyst formation rate of the reconstructed embryos were not significantly different between the fetal (80.7% and 15.6%) and newborn ear skin (77.5% and 12.3%) fibroblast groups (p>0.05), but in both groups these indices were significantly higher than that found in the adult ear skin (70.5% and 8.8%; p<0.05). Reconstructed embryos derived from fetal, newborn, and adult ear skin fibroblasts were transferred to four surrogates, respectively. For the fetal, newborn, and adult ear skin fibroblasts, the number of pregnancies were two (50.0%), two (50.0%), and one (25.0%), respectively, and the number of deliveries were two (50.0%), one (25.0%), and zero (0.0%), respectively. Seven and two cloned piglets were obtained from the fetal and newborn ear skin fibroblasts respectively, while no piglets were obtained from the adult ear skin fibroblasts. Two cloned piglets from the newborn ear skin fibroblasts died shortly after birth because of neonatal asphyxia caused by dystocia. The birth weights of the piglets derived from the fetal and newborn ear skin fibroblasts were 1230.5 and 1310.0g, respectively, which were statistically insignificant (p>0.05), but both were significantly higher than that of the control groups (p<0.05). Microsatellite analyses demonstrated that the genotypes of all cloned piglets were identical to their donor cells. Therefore, cloned pigs were successfully produced using two sources of donor cells isolated from the fetal and newborn ear skin fibroblasts of Meishan piglet, and indicating a better cloning efficiency than that obtained from adult fibroblasts. We concluded that the nuclear donor cell lines have significant impact on the developmental competence of cloned embryos as well as on the cloning efficiency of Meishan pig.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Differential expression of Toll - like receptor 4 signaling pathway genes in Escherichia coli F18 - resistant and - sensitive Meishan piglets
    Autorzy:
    Liu, Y.
    Gan, L.N.
    Qin, W.Y.
    Sun, S.Y.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31946.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The Toll-like receptor 4 (TLR4) signaling pathway is an important inflammatory pathways associated with the progression of numerous diseases. The aim of the present study was to investigate the relationship between TLR4 signaling and resistance to Escherichia coli F18 in locally weaned Meishan piglets. Using a real-time PCR approach, expression profiles were determined for key TLR4 signaling pathway genes TLR4, MyD88, CD14, IFN-α, IL-1β and TNF-α in the spleen, thymus, lymph nodes, duodenum and jejunum of E. coli F18-resistant and -sensitive animals. TLR4 signaling pathway genes were expressed in all the immune organs and intestinal tissues, and the expression was generally higher in the spleen and lymph nodes. TLR4 transcription was higher in the spleen of sensitive piglets (p<0.05), but there was no significant difference in TLR4 mRNA levels in other tissues. Similarly, CD14 transcription was higher in lymph nodes of sensitive animals (p<0.05) but not in other tissues. IL-1β expression was higher in the spleen and in the duodenum of resistant piglets (p<0.05, p<0.01, respectively), and there were no significant differences in other tissues. There were also no significant differences in the expression of MyD88, TNF-α and IFN-α between sensitive and resistant piglets (p>0.05). These results further confirm the involvement of the TLR4 signaling pathway in resistance to E. coli F18 in Meishan weaned piglets. The resistance appeared to be mediated via downregulation of TLR4 and CD14, and upregulation of MyD88 that may promote the release of cytokines TNF-α, IL-1β, IFN-α and other inflammatory mediators which help to fight against E. coli F18 infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seroprevalence of antibodies to classical swine fever virus and porcine reproductive and respiratory syndrome virus in healthy pigs in Hunan Province, China
    Autorzy:
    Yu, H.
    Zhang, L.
    Cai, Y.
    Hao, Z.
    Luo, Z.
    Peng, T.
    Liu, L.
    Wang, N.
    Wang, G.
    Deng, Z.
    Zhan, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539345.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    classical swine fever virus (CSFV)
    porcine reproductive and respiratory syndrome virus (PRRSV)
    pig
    seroprevalence
    antibody
    Opis:
    Classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS) are responsible for major economic losses and represent a threat to the swine industry worldwide. Routine surveillance serology for CSF and PRRS viruses is critical to maintaining the health status of sow farms in Hunan Province, which is one of the top pig production provinces in China. The aim of our study was to investigate the serological statistics of CSF virus (CSFV) and PRRS virus (PRRSV) in Hunan Province. The cohort serum samples were collected from vaccinated and unvaccinated pigs. Our findings showed that the average rates of CSFV and PRRSV antibody seropositivity were 82.2% (95% CI: 80.1-84.3) and 84.8% (95% CI: 82.5-87.1), respectively, in the immunized group and that these rates were higher than those in the unvaccinated group (58.6% for CSFV and 47.8% for PRRSV). Additionally, the level of CSFV antibody in piglet serum declined gradually with age, whereas PRRSV-specific antibody level increased initially (1 to 2 weeks old) and then declined with age (2 to 4 weeks old). In summary, we investigated the difference in CSFV/PRRSV antibody levels among piglets at various weeks old (1 to 4 weeks) to further establish the duration of maternal immunity in piglets. In addition, routine monitoring of CSFV/PRRSV antibodies in immunized pigs was carried out to evaluate the efficacy of vaccination.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 3; 375-381
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The distribution of different virulence grass carp reovirus strains in some neglected tissues
    Autorzy:
    Liang, H.R.
    Fu, X.Z.
    Li, N.Q.
    Liu, L.H.
    Lin, Q.
    Li, Y.G.
    Peng, Y.A.
    Huang, Z.B.
    Wu, S.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/30668.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relationship of adiponectin, leptin, visfatin and IGF-1 in cow’s venous blood and venous cord blood with calf birth weight
    Autorzy:
    Shen, L.
    Zhu, Y.
    Xiao, J.
    Deng, J.
    Peng, G.
    Zuo, Z.
    Yu, S.
    Ma, X.
    Zhong, Z.
    Ren, Z.
    Zhou, Z.
    Liu, H.
    Zong, X.
    Cao, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087491.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    adiponectin
    leptin
    IGF-1
    birth weights
    cow
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 3; 541-548
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effectson ketosis involved indices
    Autorzy:
    Shen, L.
    Qian, B.
    Xiao, J.
    Zhu, Y.
    Hussain, S.
    Deng, J.
    Peng, G.
    Zuo, Z.
    Zou, L.
    Yu, S.
    Ma, X.
    Zhong, Z.
    Ren, Z.
    Wang, Y.
    Liu, H.
    Zhou, Z.
    Cai, D.
    Hu, Y.
    Zong, X.
    Cao, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087289.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    adiponectin
    dairy cows
    ketosis
    leptin
    perinatal period
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 3; 373-381
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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