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Wyszukujesz frazę "Li, Z.-x." wg kryterium: Autor

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    Wyświetlanie 1-12 z 12
    Tytuł:
    Emerging of canine kobuvirus in dogsin China, 2015
    Autorzy:
    Liu, D.F.
    Liu, X.
    Li, Z.J.
    F. Liu
    Hu, X.L.
    Li, Z.G.
    Liu, C.G.
    Ma, J.Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087863.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    canine kobuvirus (CaKoV)
    dogs
    phylogenetic
    VP1
    China
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 4; 707-711
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
    Autorzy:
    Hu, L.
    Lin, X.Y.
    Yang, Z.X.
    Yao, X.P.
    Li, G.L.
    Peng, S.Z.
    Wang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/30249.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of a rapid immunochromatographic assay for detection of antibodies against porcine epidemic diarrhea virus
    Autorzy:
    Li, R.
    Ma, J.
    Tian, X.
    Yu, Y.
    Qiao, S.
    Wang, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087760.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    porcine epidemic diarrhea virus
    immunochromatographic test strip
    nucleocapsid protein
    diagnosis
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 139-147
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses
    Autorzy:
    Gu, X.L.
    Dai, F.Y.
    Xiao, X.
    Li, G.Z.
    Zhang, L.M.
    Qu, W.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087129.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Ageratina adenophora
    liver
    lung
    horse
    toxicity
    Źródło:
    Polish Journal of Veterinary Sciences; 2021, 24, 4; 611-613
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig
    Autorzy:
    Hua, Z.
    Xu, G.
    Liu, X.
    Bi, Y.
    Xiao, H.
    Hua, W.
    Li, L.
    Zhang, L.
    Ren, H.
    Zheng, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/31224.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for both agricultural and biomedical research. However, its efficiency is crucially dependent on the source of nuclear donor cells. In this study, we compared the cloning efficiency by using three lines of donor cells that are derived from fetal, newborn and adult fibroblasts of Chinese indigenous Meishan pig. We showed that cleavage rate and blastocyst formation rate of the reconstructed embryos were not significantly different between the fetal (80.7% and 15.6%) and newborn ear skin (77.5% and 12.3%) fibroblast groups (p>0.05), but in both groups these indices were significantly higher than that found in the adult ear skin (70.5% and 8.8%; p<0.05). Reconstructed embryos derived from fetal, newborn, and adult ear skin fibroblasts were transferred to four surrogates, respectively. For the fetal, newborn, and adult ear skin fibroblasts, the number of pregnancies were two (50.0%), two (50.0%), and one (25.0%), respectively, and the number of deliveries were two (50.0%), one (25.0%), and zero (0.0%), respectively. Seven and two cloned piglets were obtained from the fetal and newborn ear skin fibroblasts respectively, while no piglets were obtained from the adult ear skin fibroblasts. Two cloned piglets from the newborn ear skin fibroblasts died shortly after birth because of neonatal asphyxia caused by dystocia. The birth weights of the piglets derived from the fetal and newborn ear skin fibroblasts were 1230.5 and 1310.0g, respectively, which were statistically insignificant (p>0.05), but both were significantly higher than that of the control groups (p<0.05). Microsatellite analyses demonstrated that the genotypes of all cloned piglets were identical to their donor cells. Therefore, cloned pigs were successfully produced using two sources of donor cells isolated from the fetal and newborn ear skin fibroblasts of Meishan piglet, and indicating a better cloning efficiency than that obtained from adult fibroblasts. We concluded that the nuclear donor cell lines have significant impact on the developmental competence of cloned embryos as well as on the cloning efficiency of Meishan pig.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The distribution of different virulence grass carp reovirus strains in some neglected tissues
    Autorzy:
    Liang, H.R.
    Fu, X.Z.
    Li, N.Q.
    Liu, L.H.
    Lin, Q.
    Li, Y.G.
    Peng, Y.A.
    Huang, Z.B.
    Wu, S.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/30668.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus
    Autorzy:
    Lin, A.
    Hu, X.
    Cui, S.
    Yang, T.
    Zhang, Z.
    Li, P.
    Guo, M.
    Lu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647453.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Getah virus
    real-time PCR
    TaqMan
    detection
    Opis:
    To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 21-28
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    17 beta-estradiol affects proliferation and apoptosis of canine bone marrow mesenchymal stem cells in vitro
    Autorzy:
    Zhou, Z.-H.
    Gu, C.-W.
    Li, J.
    Huang, X.-Y.
    Deng, J.-Q.
    Shen, L.-H.
    Cao, S.-Z.
    Deng, J.-L.
    Zuo, Z.-C.
    Wang, Y.
    Ma, X.-P.
    Ren, Z.-H.
    Yu, S.-M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087308.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    apoptosis
    bone marrow mesenchymal stem cells (BMSCs)
    canine
    proliferation
    17 beta-estradiol (E2)
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 2; 235-245
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2
    Autorzy:
    Xia, X.J.
    Wang, L.
    Cheng, L.K.
    Shen, Z.Q.
    Li, S.G.
    Wang, J.L.
    Powiązania:
    https://bibliotekanauki.pl/articles/31256.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 20, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimal expression and purification of sapelovirus A structural protein VP1, and its immunogenicity in mice
    Autorzy:
    Zhao, T.T.
    Cui, L.
    Chen, L.
    Li, J.J.
    Liang, Q.L.
    Wu, P.J.
    Yu, X.Q.
    Zhang, Z.H.
    Hua, X.G.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087686.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    sapelovirus A
    prokaryotic expression
    purification
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 573-579
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    SYBR Green-based real-time polymerase chain reaction assay for detection of porcine parvovirus 6 in pigs
    Autorzy:
    Sun, P.
    Bai, C.X.
    Zhang, D.
    Wang, J.
    Yang, K.K.
    Cheng, B.Z.
    Li, Y.D.
    Wang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087328.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    porcine parvovirus 6
    real-time polymerase chain reaction
    SYBR Green
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 2; 197-202
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
    Autorzy:
    Li, Y.D.
    Yu, Z.D.
    Bai, C.X.
    Zhang, D.
    Sun, P.
    Peng, M.L
    Liu, H.
    Wang, J.
    Wang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087207.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Capsid gene
    PPV7
    SYBR Green I real-time PCR
    Źródło:
    Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-12 z 12

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