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Wyświetlanie 1-15 z 28
Tytuł:
Pharmacokinetics of diclofenac sodium injection in swine
Autorzy:
Yang, H.F.
Li, Y.J.
Li, Y.Y.
Huang, C.
Huang, L.X.
Bu, S.J.
Powiązania:
https://bibliotekanauki.pl/articles/2087550.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
diclofenac sodium injection
pharmacokinetics
bioavailability
swine
HPLC
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 423-426
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of IL-1β, IL-6 and TNF-αin Sprague-Dawely rats’ atrophic thymus induced by lipopolysaccharide
Autorzy:
Zhong, Y.B.
Zhang, X.L.
Lv, M.Y.
Hu, X.F.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087664.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
lipopolysaccharide
pro-inflammatory cytokines
thymus atrophy
rat
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 589-597
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, recombinant expression, and purification of osteocalcin in sika deer (Cervus nippon) antler
Autorzy:
Li, X.
Liu, M.
Bai, X.
Li, Y.
Zhao, Y.
Wang, S.
Wang, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087587.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Cervus nippon
osteocalcin
molecular cloning
expression
purification
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 143-150
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China
Autorzy:
Li, R.-f.
Tian, X.-g.
Liu, Y.
Xu, J.
Liu, D.-y.
Powiązania:
https://bibliotekanauki.pl/articles/30492.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Perspectives on novel vaccine development
Autorzy:
Wang, Y.B.
Wang, L.P.
Li, P.
Powiązania:
https://bibliotekanauki.pl/articles/2087687.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
vaccination
vaccinology
novel vaccine development
perspectives
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 643-649
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteomics profiles reveal the potential roles of proteins involved in chicken macrophages stimulated by Lipopolysaccharide
Autorzy:
Li, S.
Chen, Y.
Xue, W.
Wang, Q.
Huai, Z.
An, C.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16647586.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
chicken macrophage (HD11)
immune response
lipopolysaccharide (LPS)
proteomics
Opis:
Lipopolysaccharide (LPS), a core part of gram-negative bacteria, is crucial for inducing an inflammatory response in living things. In the current study, we used LPS from Salmonella to stimulate chicken macrophages (HD11). Proteomics was used to investigate immune-related proteins and their roles further. Proteomics investigation revealed 31 differential expression proteins (DEPs) after 4 hours of LPS infection. 24 DEPs expressions were up-regulated, while seven were down-regulated. In this investigation, ten DEPs were mainly enriched in S. aureus infection, complement, and coagulation cascades, which were all implicated in the inflammatory response and clearance of foreign pathogens. Notably, complement C3 was shown to be up-regulated in all immune-related pathways, indicating that it is a potential protein in this study. This work contributes to a better understanding and clarification of the processes of Salmonella infection in chickens. It might bring up new possibilities for treating and breeding Salmonella-infected chickens.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 2; 265-274
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vertical transmission of PCV2b to fetuses in sows intramuscularly infected with PCV2b
Autorzy:
Wang, N.D.
Li, J.J.
Wang, A.B.
Zhu, Z.
Chang, Y.
Mao, Y.
Zan, Y.
Wang, Z.F.
Deng, Z.B.
Powiązania:
https://bibliotekanauki.pl/articles/31535.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In order to investigate whether PCV2b can be transmitted across the placenta in sows thereby causing vertical infection of fetuses, six sows in 75 day of pregnancy were either intramuscularly inoculated with a PCV2b culture supernatant (n = 4) or mock infected with cell culture supernatant (n = 2). At parturition, 3 newborn piglets from each litter were randomly selected and euthanatized prior to suckling. Samples of the mesenteric lymph nodes, spleens, kidneys, hearts and lungs were collected for PCR, histopathological and immunohistochemical assays. The results showed that the newborn piglets from PCV2b-infected sows had histopathological lesions in the tested tissues. Moreover, PCV2b antigen and DNA were detected as well. These findings therefore suggested that porcine circovirus type 2b can be transmitted across the placenta of sows, thereby leading to PCV2b vertical infection of the fetuses.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Magnolol inhibits Streptococcus suis-induced inflammation and ROS formation via TLR2/MAPK/NF-κB signaling in RAW264.7 cells
Autorzy:
Wang, J.
Sun, Y.
Zhang, L.
Xu, W.
You, J.
Lu, H.
Song, Y.
Wei, J.
Li, L.
Powiązania:
https://bibliotekanauki.pl/articles/2087779.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
magnolol
Streptococcus suis
Toll-like receptor2
mitogen-activated protein kinase
nuclear factor Kappa B
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 111-118
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:
Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a blocking immunoperoxidase monolayer assay for differentiation between pseudorabies virus-infected and vaccinated animals
Autorzy:
Wang, Y.B.
Li, Y.H.
Li, Q.M.
Xie, W.T.
Guo, C.L.
Guo, J.Q.
Deng, R.G.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/2087396.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pseudorabies virus variant strains
anti-pseudorabies virus monoclonal antibody
blocking immunoperoxidase monolayer assay
differentiation between pseudorabies virus-infected and vaccinated animals
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 717-723
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
Autorzy:
Hu, L.
Lin, X.Y.
Yang, Z.X.
Yao, X.P.
Li, G.L.
Peng, S.Z.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/30249.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression analysis of multifunctional RNA-binding protein hnRNP K during development of mammalian testis
Autorzy:
Xu, H.
Zhang, P.
Li, R.
Wu, W.
Wang, S.
Xu, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087724.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
hnRNP K
testis development
spermatogenesis
pig
rat
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 343-352
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Triplex qRT-PCR with specific probe for synchronously detecting Bovine parvovirus, bovine coronavirus, bovine parainfluenza virus and its applications
Autorzy:
Geng, J.
Niu, Y.
Wei, L.
Li, Q.
Gong, Z.
Wei, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087278.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bovine parvovirus
bovine coronavirus
bovine parainfluenza virus
qRT-PCR
specificity
sensitivity
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a rapid immunochromatographic assay for detection of antibodies against porcine epidemic diarrhea virus
Autorzy:
Li, R.
Ma, J.
Tian, X.
Yu, Y.
Qiao, S.
Wang, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2087760.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
porcine epidemic diarrhea virus
immunochromatographic test strip
nucleocapsid protein
diagnosis
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 139-147
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modulation of virulence genes by the two-component system PhoP - PhoQ in avian pathogenic Escherichia coli
Autorzy:
Tu, J.
Huang, B.
Zhang, Y.
Xue, T.
Li, S.
Qi, K.
Powiązania:
https://bibliotekanauki.pl/articles/30418.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Avian pathogenic Escherichia coli (APEC) infections are a very important problem in the poultry industry. PhoP-PhoQ is a two-component system that regulates virulence genes in APEC. In this study, we constructed strains that lacked the PhoP or PhoQ genes to assess regulation of APEC pathogenicity by the PhoP-PhoQ two-component system. The PhoP mutant strain AE18, PhoQ mutant strain AE19, and PhoP/PhoQ mutant strain AE20 were constructed by the Red homologous recombination method. Swim plates were used to evaluate the motility of the APEC strains, viable bacteria counting was used to assess adhesion and invasion of chick embryo fibroblasts, and Real-Time PCR was used to measure mRNA expression of virulence genes. We first confirmed that AE18, AE19, and AE20 were successfully constructed from the wild-type AE17 strain. AE18, AE19, and AE20 showed significant decreases in motility of 70.97%, 83.87%, and 37.1%, respectively, in comparison with AE17. Moreover, in comparison with AE17, AE18, AE19, and AE20 showed significant decreases of 63.11%, 65.42%, and 30.26%, respectively, in CEF cell adhesion, and significant decreases of 59.83%, 57.82%, and 37.90%, respectively, in CEF cell invasion. In comparison with AE17, transcript levels of sodA, polA, and iss were significantly decreased in AE18, while transcript levels of fimC and iss were significantly decreased in AE19. Our results demonstrate that deletion of PhoP or PhoQ inhibits invasion and adhesion of APEC to CEF cells and significantly reduces APEC virulence by regulating transcription of virulence genes.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 28

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