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Wyszukujesz frazę "Li, Y.G." wg kryterium: Autor

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    Wyświetlanie 1-10 z 10
    Tytuł:
    The distribution of different virulence grass carp reovirus strains in some neglected tissues
    Autorzy:
    Liang, H.R.
    Fu, X.Z.
    Li, N.Q.
    Liu, L.H.
    Lin, Q.
    Li, Y.G.
    Peng, Y.A.
    Huang, Z.B.
    Wu, S.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/30668.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China
    Autorzy:
    Li, R.-f.
    Tian, X.-g.
    Liu, Y.
    Xu, J.
    Liu, D.-y.
    Powiązania:
    https://bibliotekanauki.pl/articles/30492.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of a blocking immunoperoxidase monolayer assay for differentiation between pseudorabies virus-infected and vaccinated animals
    Autorzy:
    Wang, Y.B.
    Li, Y.H.
    Li, Q.M.
    Xie, W.T.
    Guo, C.L.
    Guo, J.Q.
    Deng, R.G.
    Zhang, G.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087396.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    pseudorabies virus variant strains
    anti-pseudorabies virus monoclonal antibody
    blocking immunoperoxidase monolayer assay
    differentiation between pseudorabies virus-infected and vaccinated animals
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 4; 717-723
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    GP5 protein - based ELISA for the detection of PRRSV antibodies
    Autorzy:
    Wang, Y.
    Guo, J.
    Qiuao, S.
    Li, Q.
    Yang, J.
    Jin, Q.
    Zhang, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/30629.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Porcine reproductive and respiratory syndrome virus (PRRSV) is an important swine pathogen, causing huge economic losses each year worldwide. Immunization with vaccines containing the glycoprotein 5 (GP5) of PRRSV is the main measure to induce neutralizing antibodies and control the disease. Here, we developed a GP5 protein-based ELISA for detecting antibodies against PRRSV. The overall yield of purified GP5 in E. coli flask culture was more than 45 mg/L cell culture. Western blot and IFA indicated that the GP5 protein was highly immunogenic. After optimization and validation with IDEXX PRRS using 566 clinical sera, the DSN, DSP, and accuracy of GP5-ELISA were 81.39%, 75.96%, and 80.39%, respectively. Besides, GP5-ELISA is highly specific, showing no cross-reactions with sera against other important swine pathogens. Hence, GP5 is a good diagnostic antigen and the GP5 protein-based ELISA has the potential to be used in the field
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
    Autorzy:
    Hu, L.
    Lin, X.Y.
    Yang, Z.X.
    Yao, X.P.
    Li, G.L.
    Peng, S.Z.
    Wang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/30249.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bacterial communities in PM2.5 and PM10 in broiler houses at different broiler growth stages in spring
    Autorzy:
    Zhang, J.
    Li, Y.
    Xu, E.
    Jiang, L.
    Tang, J.
    Li, M.
    Zhao, X.
    Chen, G.
    Zhu, H.
    Yu, X.
    Zhang, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087465.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bacterial communities
    broilers
    high-throughput sequencing
    particulate matter
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 3; 495-504
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
    Autorzy:
    Dai, G.
    Huang, X.
    Liu, Q.
    Li, Y.
    Zhang, L.
    Han, K.
    Yang, J.
    Liu, Y.
    Xue, F.
    Zhao, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647377.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    epitope
    goose astrovirus
    capsid protein
    monoclonal antibody
    Opis:
    Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
    Autorzy:
    Jin, Q.Y.
    Feng, L.L.
    Wang, Y.B.
    Li, P.
    Yang, J.F.
    Teng, M.
    Chai, S.J.
    Xing, G.X.
    Zhang, G.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539073.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    porcine circovirus type 2 (PCV2)
    screening of MAbs
    paper test
    Opis:
    A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses
    Autorzy:
    Gu, X.L.
    Dai, F.Y.
    Xiao, X.
    Li, G.Z.
    Zhang, L.M.
    Qu, W.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087129.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Ageratina adenophora
    liver
    lung
    horse
    toxicity
    Źródło:
    Polish Journal of Veterinary Sciences; 2021, 24, 4; 611-613
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig
    Autorzy:
    Hua, Z.
    Xu, G.
    Liu, X.
    Bi, Y.
    Xiao, H.
    Hua, W.
    Li, L.
    Zhang, L.
    Ren, H.
    Zheng, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/31224.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for both agricultural and biomedical research. However, its efficiency is crucially dependent on the source of nuclear donor cells. In this study, we compared the cloning efficiency by using three lines of donor cells that are derived from fetal, newborn and adult fibroblasts of Chinese indigenous Meishan pig. We showed that cleavage rate and blastocyst formation rate of the reconstructed embryos were not significantly different between the fetal (80.7% and 15.6%) and newborn ear skin (77.5% and 12.3%) fibroblast groups (p>0.05), but in both groups these indices were significantly higher than that found in the adult ear skin (70.5% and 8.8%; p<0.05). Reconstructed embryos derived from fetal, newborn, and adult ear skin fibroblasts were transferred to four surrogates, respectively. For the fetal, newborn, and adult ear skin fibroblasts, the number of pregnancies were two (50.0%), two (50.0%), and one (25.0%), respectively, and the number of deliveries were two (50.0%), one (25.0%), and zero (0.0%), respectively. Seven and two cloned piglets were obtained from the fetal and newborn ear skin fibroblasts respectively, while no piglets were obtained from the adult ear skin fibroblasts. Two cloned piglets from the newborn ear skin fibroblasts died shortly after birth because of neonatal asphyxia caused by dystocia. The birth weights of the piglets derived from the fetal and newborn ear skin fibroblasts were 1230.5 and 1310.0g, respectively, which were statistically insignificant (p>0.05), but both were significantly higher than that of the control groups (p<0.05). Microsatellite analyses demonstrated that the genotypes of all cloned piglets were identical to their donor cells. Therefore, cloned pigs were successfully produced using two sources of donor cells isolated from the fetal and newborn ear skin fibroblasts of Meishan piglet, and indicating a better cloning efficiency than that obtained from adult fibroblasts. We concluded that the nuclear donor cell lines have significant impact on the developmental competence of cloned embryos as well as on the cloning efficiency of Meishan pig.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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