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Wyświetlanie 1-15 z 16
Tytuł:
Effect of bamboo vinegar powder on the expression of the immune-related genes MyD88 and CD14 in weaning piglets
Autorzy:
Qin, W.Y.
Gan, L.N.
Dong, L.
Yu, L.H.
Wu, S.L.
Bao, W.B.
Powiązania:
https://bibliotekanauki.pl/articles/2087674.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bamboo vinegar powder
pig
antibiotics
MyD88 gene
CD14 gene
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 525-532
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
LBP gene methylation involved in mRNA expression and resistance to E. coli F18 in weaned piglets
Autorzy:
Gan, L.N.
Bao, W.B.
Wu, S.L.
Qin, W.Y.
Sun, L.
Zhao, C.X.
Powiązania:
https://bibliotekanauki.pl/articles/2087872.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
LBP gene
methylation analysis of the LBP region
E.coli F18 strain
weaned piglets
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 643-650
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differential expression of Toll - like receptor 4 signaling pathway genes in Escherichia coli F18 - resistant and - sensitive Meishan piglets
Autorzy:
Liu, Y.
Gan, L.N.
Qin, W.Y.
Sun, S.Y.
Zhu, G.Q.
Wu, S.L.
Bao, W.B.
Powiązania:
https://bibliotekanauki.pl/articles/31946.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The Toll-like receptor 4 (TLR4) signaling pathway is an important inflammatory pathways associated with the progression of numerous diseases. The aim of the present study was to investigate the relationship between TLR4 signaling and resistance to Escherichia coli F18 in locally weaned Meishan piglets. Using a real-time PCR approach, expression profiles were determined for key TLR4 signaling pathway genes TLR4, MyD88, CD14, IFN-α, IL-1β and TNF-α in the spleen, thymus, lymph nodes, duodenum and jejunum of E. coli F18-resistant and -sensitive animals. TLR4 signaling pathway genes were expressed in all the immune organs and intestinal tissues, and the expression was generally higher in the spleen and lymph nodes. TLR4 transcription was higher in the spleen of sensitive piglets (p<0.05), but there was no significant difference in TLR4 mRNA levels in other tissues. Similarly, CD14 transcription was higher in lymph nodes of sensitive animals (p<0.05) but not in other tissues. IL-1β expression was higher in the spleen and in the duodenum of resistant piglets (p<0.05, p<0.01, respectively), and there were no significant differences in other tissues. There were also no significant differences in the expression of MyD88, TNF-α and IFN-α between sensitive and resistant piglets (p>0.05). These results further confirm the involvement of the TLR4 signaling pathway in resistance to E. coli F18 in Meishan weaned piglets. The resistance appeared to be mediated via downregulation of TLR4 and CD14, and upregulation of MyD88 that may promote the release of cytokines TNF-α, IL-1β, IFN-α and other inflammatory mediators which help to fight against E. coli F18 infection.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of fluorescence quantitative polymerase chain reaction (PCR) for the detection of Escherichia coli adhesion to pig intestinal epithelial cells
Autorzy:
Dai, C.H.
Gan, L.N.
Qin, W.U.
Zi, C.
Zhu, G.Q.
Wu, S.L.
Bao, W.B.
Powiązania:
https://bibliotekanauki.pl/articles/31510.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
An efficient and accurate method to test Escherichia coli (E. coli) adhesion to intestinal epithelial cells will contribute to the study of bacterial pathogenesis and the function of genes that encode receptors related to adhesion. This study used the quantitative real-time polymerase chain reaction (qPCR) method. qPCR primers were designed from the PILIN gene of E. coli F18ab, F18ac, and K88ac, and the pig β-ACTIN gene. Total deoxyribonucleic acid (DNA) from E. coli and intestinal epithelial cells (IPEC-J2 cells) were used as templates for qPCR. The 2−ΔΔCt formula was used to calculate the relative number of bacteria in cultures of different areas. We found that the relative numbers of F18ab, F18ac, and K88ac that adhered to IPEC-J2 cells did not differ significantly in 6-, 12-, and 24-well culture plates. This finding indicated that there was no relationship between the relative adhesion number of E. coli and the area of cells, so the method of qPCR could accurately test the relative number of E. coli. This study provided a convenient and reliable testing method for experiments involving E. coli adhesion, and also provided innovative ideas for similar detection methods.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in physiological and immunological parameters during the peripartum period in Zerasca sheep
Autorzy:
Giuliotti, L.
Lai, O.
Cavallina, R.
Alfieri, L.
Rabusca, G.
Benvenuti, M.N.
Powiązania:
https://bibliotekanauki.pl/articles/32191.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to provide the picture of the dynamics of some blood parameters in the native Zerasca sheep breed during the peripartum period. Blood samples were collected from peripheral blood of 14 ewes at different times: from 21 d before lambing to 42 d after. Physiological and immunological parameters were evaluated: aspartate-aminotransferase, creatine kinase, lactate dehydrogenase, non-esterified fatty acids, total protein, total antioxidant capacity, free radicals, serum bactericidal activity and serum lysozyme. One-way repeated measures ANOVA test was performed. Results showed a significant influence of the peripartum and the deviation from the normal range on many parameters.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of dietary supplementation with Echinacea purpurea on vaccine efficacy against infection with Flavobacterium columnare in zebrafish (Danio rerio)
Autorzy:
Guz, L.
Puk, K.
Walczak, N.
Oniszczuk, T.
Oniszczuk, A.
Powiązania:
https://bibliotekanauki.pl/articles/32291.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The effect of dietary Echinacea purpurea (EP) on the response of zebrafish (Danio rerio) to a Flavobacterium columnare vaccine was investigated. Two hundred D. rerio with an average weight of 290 ± 40 g were selected and fed different levels of E. purpurea (5 g kg⁻¹ diet - group 1, 10 g kg⁻¹ diet - group 2, 20 g kg⁻¹ diet - group 3, 30 g kg⁻¹ diet - group 4, and 0 g kg⁻¹ diet - group 5). Experimental feeding was begun 3 weeks prior to bath immunization and continued until the end of the experiment. Twenty-eight days after immunization the fish were challenged by bath immersion with F. columnare at a concentration of 1x106 CFU/ml. The relative percent survival of the experimental groups (1, 2, 3, 4 and 5) was 5.0, 6.0, 30.0, 36.0 and 5.0, respectively. In conclusion, diet supplementation with E. purpurea may effectively enhance the response of zebrafish to a F. columnare vaccine.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antioxidant supplementation to medium for in vitro embryo production in Felis catus
Autorzy:
Cocchia, N.
Tafuri, S.
Del Prete, C.
Palumbo, V.
Esposito, L.
Avallone, L.
Ciani, F.
Powiązania:
https://bibliotekanauki.pl/articles/2087464.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
assisted reproduction technology
superoxide dismutase
glutathione peroxidase
catalase
domestic cat
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 573-579
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli
Autorzy:
Zhao, L.Y.
Niu, J.H.
Gao, X.L.
Liu, C.N.
Liu, S.M.
Jiang, N.
Lv, X.P.
Zheng, S.M.
Powiązania:
https://bibliotekanauki.pl/articles/2087340.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Escherichia coli
F4 fimbriae
isothermal amplification
rapid detection
clinical samples
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 143-152
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunophenotypic characteristics and karyotype analysis of bone marrow-derived mesenchymal stem cells of rabbits duringin vitro cultivation
Autorzy:
Mazurkevych, A.
Kharkevych, Y.
Jakubczak, A.
Gryzinska, M.
Malyuk, M.
Bezdieniezhnykh, N.
Starodub, L.
Powiązania:
https://bibliotekanauki.pl/articles/2087868.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mesenchymal stem cells
monoclonal antibodies
E-cadherin
N-cadherin
actin
vimentin
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 687-695
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Calcium (Ca2+) expression and intensity in cumulus-oocyte complex (COCs) in Kacang goat after vitrification
Autorzy:
Widjiati, W.
Faizah, Z.
Darsini, N.
Hendrawan, V.F.
Karima, H.N.
Chotimah, C.
Sumitro, S.B.
Yustinasari, L.R.
Kasman, A.A.M.N.
Ntoruru, J.M.
Luqman, E.M.
Powiązania:
https://bibliotekanauki.pl/articles/16539072.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Calcium (Ca2+)
confocal laser scanning microscope
cumulus-oocyte complex (COCs)
food production
Kacang goat
Opis:
The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+ in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+ and the intensity of Ca2+ in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+ was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+ in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+ in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+ between fresh COCs and COCs after vitrification. Ca2+ intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 19-26
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hypertonic saline solution (NaCl 7.2%) enhances renal excretion of acids in cattle with acute ruminal lactic acidosis
Autorzy:
Rodrigues, F.A.M.L.
Minervino, A.H.H.
Barrêto Júnior, R.A.
Reis, L.F.
Ferreira, R.N.F.
Mori, C.S.
Oliveira, F.L.C.
Sousa, R.S.
Powiązania:
https://bibliotekanauki.pl/articles/2087583.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
steers
ruminal lactic acidosis
hypertonic saline solution
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 37-42
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of antibodies to classical swine fever virus and porcine reproductive and respiratory syndrome virus in healthy pigs in Hunan Province, China
Autorzy:
Yu, H.
Zhang, L.
Cai, Y.
Hao, Z.
Luo, Z.
Peng, T.
Liu, L.
Wang, N.
Wang, G.
Deng, Z.
Zhan, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16539345.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
classical swine fever virus (CSFV)
porcine reproductive and respiratory syndrome virus (PRRSV)
pig
seroprevalence
antibody
Opis:
Classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS) are responsible for major economic losses and represent a threat to the swine industry worldwide. Routine surveillance serology for CSF and PRRS viruses is critical to maintaining the health status of sow farms in Hunan Province, which is one of the top pig production provinces in China. The aim of our study was to investigate the serological statistics of CSF virus (CSFV) and PRRS virus (PRRSV) in Hunan Province. The cohort serum samples were collected from vaccinated and unvaccinated pigs. Our findings showed that the average rates of CSFV and PRRSV antibody seropositivity were 82.2% (95% CI: 80.1-84.3) and 84.8% (95% CI: 82.5-87.1), respectively, in the immunized group and that these rates were higher than those in the unvaccinated group (58.6% for CSFV and 47.8% for PRRSV). Additionally, the level of CSFV antibody in piglet serum declined gradually with age, whereas PRRSV-specific antibody level increased initially (1 to 2 weeks old) and then declined with age (2 to 4 weeks old). In summary, we investigated the difference in CSFV/PRRSV antibody levels among piglets at various weeks old (1 to 4 weeks) to further establish the duration of maternal immunity in piglets. In addition, routine monitoring of CSFV/PRRSV antibodies in immunized pigs was carried out to evaluate the efficacy of vaccination.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 375-381
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genome-wide association studies of cryostability of semen in roosters
Autorzy:
Dementieva, N.V.
Kudinov, A.A.
Pozovnikova, M.V.
Nikitkina, E.V.
Pleshanov, N.V.
Silyukova, Y.L.
Krutikova, A.A.
Plemyashov, K.V.
Powiązania:
https://bibliotekanauki.pl/articles/2087292.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Genome-Wide Association Studies
cryostability
rooster sperm
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 461-463
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induction of periodontal disease via retentive ligature, lipopolysaccharide injection, and their combination in a rat model
Autorzy:
Mustafa, H.
Cheng, C.H.
Radzi, R.
Fong, L.S.
Mustapha, N.M.
Dyary, H.O.
Powiązania:
https://bibliotekanauki.pl/articles/2087105.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
alveolar bone
periodontal disease
Porphyromonas gingivalis lipopolysaccharide
retentive ligature
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 3; 365-373
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An in vitro assessment of antiviral activity for ethanol extract of Desmodium canadense against bovine herpesvirus type 1
Autorzy:
Lelešius, R.
Girdauskaitė, P.
Karpovaitė, A.
Mickienė, R.
Drevinskas, T.
Tiso, N.
Ragažinskienė, O.
Kubilienė, L.
Maruška, A.
Šalomskas, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087312.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bovine herpesvirus 1
plant extracts
Desmodium canadense
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 177-184
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 16

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