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Wyświetlanie 1-15 z 37
Tytuł:
Development of a blocking immunoperoxidase monolayer assay for differentiation between pseudorabies virus-infected and vaccinated animals
Autorzy:
Wang, Y.B.
Li, Y.H.
Li, Q.M.
Xie, W.T.
Guo, C.L.
Guo, J.Q.
Deng, R.G.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/2087396.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pseudorabies virus variant strains
anti-pseudorabies virus monoclonal antibody
blocking immunoperoxidase monolayer assay
differentiation between pseudorabies virus-infected and vaccinated animals
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 717-723
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of a hydrolysed salmon and pea hypoallergenic diet application in dogs and cats with cutaneous adverse food reaction
Autorzy:
Szczepanik, M.P.
Gołyński, M.
Wilkołek, P.
Kalisz, G.
Powiązania:
https://bibliotekanauki.pl/articles/16539080.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
cats
cutaneous adverse food reaction
dogs
hypoallergenic diet
Opis:
Cutaneous adverse food reaction (CAFR) is a common disease, affecting about 1-2% of dogs and cats. Diagnosis of the CAFR is made through elimination diet coupled with diet challenge, as methods like skin tests, patch tests, basophil degranulation tests and assessment of IgG and IgE serum levels are not sensitive enough. A partially hydrolysed salmon and pea hypoallergenic diet was evaluated in the diagnosis and treatment of CAFR in dogs and cats. The diet was used in the treatment of 13 dogs and 12 cats for 10 weeks. The Pruritus Visual Analog Scale (PVAS; dogs and cats), Canine Atopic Dermatitis Extent and Severity Index (CADESI-04; dogs) and the Scoring Feline Allergic Dermatitis (SCORFAD; cats) were used for effectiveness evaluation. In dogs, a significant decrease was reported in both CADESI-04 (from 17.3±7.5 to 10.15±7.4; p=0.028) and PVAS (from 7±1.3 to 4.76±1.8; p=0.003) after four weeks of treatment. Also in cats, both the PVAS (from 6.75±1.8 to 4±2.3; p=0.006) and SCORFAD (from 4.16±1.9 to 2.58±1.2; p=0.029) decreased significantly after four weeks. After eight weeks, a significant improvement was observed in almost all the animals. Evaluated diet was useful in the treatment of the CAFR in dogs and cats.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 67-73
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of elements of the Stewart model (Strong Ion Approach) - SID3, SID4, Atot/Aminus, SIDe and SIG for the diagnostics of respiratory acidosis in brachycephalic dogs
Autorzy:
Slawuta, P.
Sapikowski, G.
Sobieraj, B.
Powiązania:
https://bibliotekanauki.pl/articles/31951.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Buffer systems of blood and tissues, which have the ability to bind with and give up hydrogen ions, participate in maintaining the acid-base balance (ABB) of the organism. According to the classic model, the system of carbonic acid and bicarbonates, where the first component serves the role of an acid and the second a base, determines plasma pH. The so-called Stewart model, which assumes that ions in blood serum can be separated into completely dissociated – nonbuffer and not dissociated – buffer ions which may give up or accept H+ions, also describes the ABB of the organism. The goal of the study was to find out whether, during respiratory acidosis, the values of SID3, SID4, Atot/A−, SIDe and SIG change. The study was carried out on 60 adult dogs of the boxer breed (32 males and 28 females) in which, on the basis of an arterial blood test, respiratory acidosis was found. A strong overgrowth of the soft palate tissue requiring a surgical correction was the cause of the ABB disorder. Prior to surgery and on the 14th day after the surgery, venous and arterial blood was drawn from each dog. ABB parameters were determined in the arterial blood sample: the blood pH, pCO2 and HCO3−. In the venous blood, concentration of Na+, K+, Cl−, lactate−, albumins, and Pinorganic was determined. On the basis of the obtained data, the values of SID3, SID4, SIDe, A− and SIG, before and after the surgery, were calculated. In spite of the fact that the average concentration of ions, albumins, Pinorganic and lactate in the blood serum of dogs before and after the surgical procedure was similar and within the physiological norms, the values of SID3, SIDe and SIG, calculated on the basis of the former, displayed statistically significant differences. Conclusion: On the basis of the results obtained, it can be stated that the values of SID3, SIDe and SIG change during respiratory acidosis and may be helpful in the diagnostics of ABB disorders in brachycephalic dogs.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Secretory function of adipose tissue
Autorzy:
Kuryszko, J.
Slawuta, P.
Sapikowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/32531.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
There are two kinds of adipose tissue in mammals: white adipose tissue – WAT and brown adipose tissue – BAT. The main function of WAT is accumulation of triacylglycerols whereas the function of BAT is heat generation. At present, WAT is also considered to be an endocrine gland that produces bioactive adipokines, which take part in glucose and lipid metabolism. Considering its endocrine function, the adipose tissue is not a homogeneous gland but a group of a few glands which act differently. Studies on the secretory function of WAT began in 1994 after discovery of leptin known as the satiation hormone, which regulates body energy homeostasis and maintainence of body mass. Apart from leptin, the following belong to adipokines: adiponectin, resistin, apelin, visfatin and cytokines: TNF and IL 6. Adiponectin is a polypeptide hormone of antidiabetic, anti-inflammatory and anti-atherogenic activity. It plays a key role in carbohydrate and fat metabolism. Resistin exerts a counter effect compared to adiponectin and its physiological role is to maintain fasting glycaemia. Visfatin stimulates insulin secretion and increases insulin sensitivity and glucose uptake by muscle cells and adipocytes. Apelin probably increases the insulin sensitivity of tissues. TNF evokes insulin resistance by blocking insulin receptors and inhibits insulin secretion. Approximately 30% of circulating IL 6 comes from adipose tissue. It causes insulin resistance by decreasing the expression of insulin receptors, decreases adipogenesis and adiponectin and visfatin secretion, and stimulates hepatic gluconeogenesis. In 2004, Bays introduced the notion of adiposopathy, defined as dysfunction of the adipose tissue, whose main feature is insulin and leptin resistance as well as the production of inflammatory cytokines: TNF and IL 6 and monocyte chemoattractant protein. This means that excess of adipose tissue, especially visceral adipose tissue, leads to the development of a chronic subclinical inflammatory condition, which favours the development of insulin resistance and Type 2 diabetes. Obesity is a systemic illness caused by energy transformation homeostasis disorder which results in an increase in the amount of body fat mass. It effects approximately 40% of dogs and 20% of cats. Illnesses which accompany obesity result, to a great extent, from the secretive role of adipose tissue, which is still little known, which should be included when planning treatment of an obese animal.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Simvastatin modulates β-catenin/MDR1 expression on spheres derived from CF41.Mg canine mammary carcinoma cells
Autorzy:
Cruz, P.
Reyes, F.
Torres, C.G.
Powiązania:
https://bibliotekanauki.pl/articles/2087784.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
β-catenin
MDR1
simvastatin
canine mammary carcinoma cells
cancer stem cells
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 95-99
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphology of immune organs after very virulent plus strain of Marek’s disease virus infection in vaccinated hens
Autorzy:
Madej, J.P.
Wozniakowski, G.
Gawel, A.
Powiązania:
https://bibliotekanauki.pl/articles/31459.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Marek’s disease (MD) outbreaks in poultry flocks may be associated with overriding of vaccine immune protection by very virulent (vvMDV) or very virulent plus (vv+MDV) strains. This paper presents the study on lymphoid organ morphology in the latent phase of MD caused by vv+MDV which break post-vaccinal protection in hens. We also immunohistochemically examined B and T populations as well as B/T and CD4+/CD8+ ratio of lymphocytes in lymphatic organs and, as a background, in MD lymphomas from non-lymphatic organs. The number of antigen expressed cells was evaluated as a percentage of positive cells in the one power field. Organ samples were collected from 24 dead reproductive hens (Ross 308 line) in age between 35-56 weeks, infected with vv+MDV. The hens originated from farms with MD outbreaks, despite earlier routine vaccination with CVI988/Rispens + HVT. The control organ samples originated from 15 clinically healthy hens at the same age and line, subjected to the same vaccination schedule. The number of CD3+, CD8+ and TCRγδ+ cells was significantly lower in MDV infected thymus, spleen and cecal tonsils in comparison to that found in the control organs. The proportion of CD4+ was also distinctly reduced in the thymus and limited in the spleen of MDV infected hens. This study revealed that infection with field vv+MDV isolates might break post-vaccinal protection and influence the central and peripheral immune system. The decrease in CD8+ and TCRγδ+ cell number in the thymus, spleen and cecal tonsils suggests that primarily these cells are involved in cell-mediated cytotoxicity against MDV transformed cells during latency.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effectiveness of modified seton and modified combat gauze in controlling severe hemorrhaging during operations of uniformed services
Autorzy:
Adamiak, Z.
Bulowiecka, D.
Jestrzebski, P.
Jalynski, M.
Holak, P.
Glodek, J.
Gudzbeler, G.
Powiązania:
https://bibliotekanauki.pl/articles/32377.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Hemorrhaging from large vessels poses a serious problem in emergency situations when blood loss needs to be immediately controlled. The aim of the study was to compare the effectiveness of two hemostatic dressings in controlling bleeding from a surgically punctured femoral artery. The study was performed on thirteen pigs divided into two groups, of six and seven pigs, respectively. Combat gauze covered with ChitoClear hqg 95 chitosan and Protanal LF10/60 FT sodium alginate was used in the first group, seton covered with identical substances was uses in the second group. Selected hemostatic dressing was applied to the wound 20 seconds after incision and then removed at regular time intervals to evaluate hemostasis. Modified seton was characterized by a shorter time to hemostasis than combat gauze. The result of this experiment indicate that modified seton proved to be a more effective dressing than modified combat gauze.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Response of the coagulation system after application of hemostatic dressings in an animal model
Autorzy:
Jastrzebski, P.
Adamiak, Z.
Pomianowski, A.
Krystkiewicz, W.
Holak, P.
Sawicki, S.
Przyborowska, P.
Zhalniarovich, Y.
Gudzbeler, G.
Powiązania:
https://bibliotekanauki.pl/articles/30833.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of this study was to determine the response of hemostatic dressings. Coagulation and fibrinolytic systems, red blood cell parameters, platelet and leukocyte counts were evaluated after the application of hemostatic dressings: QuikClot, Chitoauze and Celox gauze. The experiment was performed on ten pigs
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Therapeutic activity of chlorhexidine-poloxamer antiseptic gel on wound healing in rats: a preclinical study
Autorzy:
Babickaite, L.
Grigonis, A.
Ramanauskiene, K.
Matusevicius, A.P.
Zamokas, G.
Daunoras, G.
Ivaskiene, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087789.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
skin
wound
chlorhexidine
poloxamer
healing
gel
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 175-183
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pre-analytical factors affect the accurate measurement of testosterone concentrations in plasma and serum of goats
Autorzy:
Gholib, G.
Wahyuni, S.
Abdilla, A.
Nugraha, T.P.
Powiązania:
https://bibliotekanauki.pl/articles/2087113.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
centrifugation time
collection time
ELISA
goats
sample storage
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 3; 355-363
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of lumbosacral transitional vertebrae in dogs in Berlin
Autorzy:
Gong, H.
Slunsky, P.
Klass, L.G.
Brunnberg, L.
Powiązania:
https://bibliotekanauki.pl/articles/2087324.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
radiology
congenital disease
breed health
hip dysplasia
cauda equina
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 261-265
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of dietary fibre on metabolism and performance in sows
Autorzy:
Papatsiros, V.G.
Katsarou, M.-S.
Drakoulis, N.
Maragkakis, G.
Tzika, E.
Maes, D.
Tassis, P.D.
Lagiou, M.
Christodoulopoulos, G.
Powiązania:
https://bibliotekanauki.pl/articles/2087173.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
insulin
ghrelin
leptin
fibre
dysgalactia
pig
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 271-279
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The efficacy of lactic acid bacteria Pediococcus acidilactici, lactose and formic acid as dietary supplements for turkeys
Autorzy:
Wajda, S
Smiecinska, K.
Jankowski, J.
Matusevicius, P.
Buteikis, G.
Powiązania:
https://bibliotekanauki.pl/articles/31170.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
animal production
animal feeding
turkey
probiotic
lactose
lactic acid bacteria
Pediococcus acidilactici
formic acid
dietary supplement
feed supplementation
slaughter value
meat quality
Opis:
A feeding trial was performed on 1400 Big-6 turkey toms divided into experimental groups subject to the use of dietary supplements. The ain of this study was to evaluate the efficacy of the probiotic supplement Bactocell, containing lactic acid bacteria Pediococcus acidilactici, and lactose, administered to turkeys separately or in combination, as well as a formic acid supplement. The addition of the probiotic under test (lactic acid bacteria Pediococcus acidilactici) to diets for turkeys contributed to higher daily gains and lower feed consumption per kg weigh gain only during the first 12 weeks of their life. Diet supplementation with lactic acid bacteria and lactose reduced mortality rates. A slaughter value analysis revealed only a slightly (by approximately 1%) higher content of breast muscle and a lower content of thigh muscle in birds fed diets supplemented with lactic acid bacteria. Turkeys receiving lactic acid bacteria or lactose and a combination of both these supplements were characterized by a higher fat content of meat and slightly lower pH values, whereas meat from turkeys fed lactose-supplemented diets was darker in color. The addition of formic acid Acidum formicum to diets for turkeys contributed only to lower mortality rates.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 45-51
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An attempt to use the serum concentration of the phosphate (Pi) and the Ca x P productas markers of the progression of chronic kidney disease in cats
Autorzy:
Sławuta, P.
Kumiega, E.
Sikorska-Kopyłowicz, A.
Sapikowski, G.
Kurosad, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087386.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chronic kidney disease (CKD)
serum phosphate concentration
serum Ca x Pproduct
cats
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 647-652
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 37

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