Autor: Feng, X. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Effect of matrine on reducing damage to bovine mammary epithelial cells induced by Staphylococcus aureus alpha-hemolysin
Autorzy:: Feng, F.
Ma, W.W.
Luo, H.X.
Guan, C.P.
Zhou, X.Z.
Powiązania:: https://bibliotekanauki.pl/articles/2087720.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: α-hemolysin
BMECs
matrine
cell damage
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 2; 409-413
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Evaluation of the protective efficacy of virus-like particles based on PCV 2b and 2d subtypes against mixed challenge in mice
Autorzy:: Yuan, X.M.
Yuan, Q.C.
Feng, S.M.
Deng, Z.B.
Powiązania:: https://bibliotekanauki.pl/articles/16539142.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: type 2
virus-like particles
vaccine
mouse
Opis:: Porcine circovirus type 2 (PCV2) is an economically important swine pathogen and, although small, it has the highest evolution rate among DNA viruses. Commercial PCV2 commercial vaccines are inactivated PCV2 isolates or a subunit vaccine based on the Cap protein of PCV2. Currently, PCV2 VLPs of individual subtype vaccines are available. Although the main prevalent genotype worldwide is PCV2b, the emerging subtype PCV2d subtype is also increasingly associated with PCV disease. The aim of the study was to evaluate the protective efficacy of VLP based on the PCV2b and 2d subtypes against the mixed challenge of two hypotype PCV2 in mice. Thirty-six female SPV Kunming mice were immunized twice with PCV2b and 2d VLPs, then challenged with PCV2b and PCV2d, to assess the immunogenicity and effectiveness of the VLPs. Vaccination of the mice with PCV2b and 2d VLPs elicited a robust antibody response specific for the PCV2. The virus load detected in the 2b and 2d spleen vaccine group was the lowest compared to other groups. Furthermore, there was no pathological damage in the HE stained sections of the 2b and 2d spleen vaccine, and no virus was detected in the immunohistochemical sections. Our data suggest that the mixed PCV2b and 2d VLP vaccine could protect mice from challenge with the mixed infection of PCV2b and PCV2d.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 2; 195-205
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Serological surveillance of avian influenza virus and canine distemper virus in captive Siberian Tigers in Northeastern China
Autorzy:: Wang, K.
Wang, H.
Feng, N.
Zhao, Y.
Gao, Y.
Hu, G.
Xia, X.
Powiązania:: https://bibliotekanauki.pl/articles/2087698.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: influenza A virus
canine distemper virus
serological
Siberian Tiger
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 3; 491-495
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: TaqMan real-time PCR for detecting bovine viral diarrhea virus
Autorzy:: Liang, H.
Geng, J.
Bai, S.
Aimuguri, A.
Gong, Z.
Feng, R.
Shen, X.
Wei, S.
Powiązania:: https://bibliotekanauki.pl/articles/2087557.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: bovine viral diarrhea virus
quantitative real time PCR
TaqMan probe
Źródło:: Polish Journal of Veterinary Sciences; 2019, 2; 405-413
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:: Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:: https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:: A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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