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Wyszukujesz frazę "Adaszek, M." wg kryterium: Autor

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    Wyświetlanie 1-15 z 15
    Tytuł:
    Detection of Borrelia burgdorferi sensu lato DNA in the blood of wild bison from Bialowieza Primeval Forest in eastern Poland
    Autorzy:
    Adaszek, L.
    Dziegiel, B.
    Krzysiak, M.
    Skrzypczak, M.
    Adaszek, M.
    Staniec, M.
    Wylupek, D.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/31883.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of the present study was to investigate the occurrence of Borrelia burgdorferi sensu lato DNA in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland. The PCR technique revealed the presence of 16S RNA of Borrelia burgdorferi sensu lato in the blood of 16 out of 120 examined animals. DNA amplification by means of primers SC1 and SC2 gave a product with a size of 300-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with B. burgdorferi s.l. 16S RNA gene DQ111061. Results of this study suggest that wild bison are important in maintaining agents of Lyme borreliosis, and that studies of reservoir competence of this species are indicated.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular identification of Babesia spp isolated from Polish cattle with asymptomatic protozoa infections
    Autorzy:
    Staniec, M.
    Adaszek, Ł.
    Buczek, K.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087739.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    babesiosis
    cattle
    sequencing
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 287-291
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Lyme disease in Bernese Mountain Dogs. Is it a real problem?
    Autorzy:
    Adaszek, Ł.
    Pisarek, M.
    Kalinowski, M.
    Skrzypczak, M.
    Winiarczyk, M.
    Abramowicz, B.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647394.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Lyme disease
    vector-borne diseases
    dogs
    Opis:
    Borreliosis is the most frequently diagnosed tick-borne disease caused by spirochete bacteria belonging to the genus Borreliae - Borrelia burgdorferi sensu stricto (s.s.), Borrelia afzelii and Borrelia garinii. Clinical manifestations in dogs include fever, lameness, polyarthritis and glomerulonephritis. Diagnosis is mainly serological and is based on an immunoenzymatic test followed by a Western blot confirmatory test. Early treatment with antibiotics such as doxycycline or amoxicillin, for four weeks, usually reduces the risk of chronic disease. Tick control, including tick repellents, is highly reliable in preventing transmission. Vaccines are available to reduce transmission and the clinical manifestations of infection in dogs. Bernese Mountain Dogs are a breed that often test positive for antibodies against B. burgdorferi without showing any clinical symptoms of the disease. Quantitative determination of the immunoglobulin level for spirochetes has indicated that Bernese Mountain Dogs may have an increased susceptibility to Borrelia spp. infections of a hereditary nature.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 639-647
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The identification of Anaplasma spp. isolated from fallow deer (Dama dama) on a free-range farm in eastern Poland
    Autorzy:
    Adaszek, L.
    Klimiuk, P.
    Skrzypczak, M.
    Gorna, M.
    Zietek, J.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/32373.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of the present study was to investigate the occurrence of Anaplasma spp. in group of 50 fallow deer (Dama dama) from free-range farm in eastern Poland and determine what species of Anaplasma could infect these animals based on PCR gene sequencing. The PCR technique revealed the presence of 16S RNA Anaplasma spp. genetic material in the blood of 7 out of 50 examined animals. The sequences of the PCR products obtained showed a 100% homology with each other and 100% homology with GU 183908 sequence of A. phagocytophilum, isolated in our earlier study from a horse with clinical form of anaplasmosis. Here, we report the first molecular evidence of Anaplasma spp. among naturally infected fallow deer in eastern Poland.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurence of Bordetella bronchiseptica in domestic cats with upper respiratory tract infections
    Autorzy:
    Garbal, M.
    Adaszek, L.
    Lyp, P.
    Frymus, J.
    Winiarczyk, M.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/30747.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Bordetella bronchiseptica is a widespread Gram-negative pathogen occurring in different mammal species. It is known to play a role in the etiology of infectious atrophic rhinitis of swine, canine kennel cough, respiratory syndromes of cats, rabbits and guinea pigs, and sporadic human cases have also been reported. The aim of this article is to present the occurrence of infections caused by these bacteria in domestic cats with respiratory symptoms, as well as to conduct a molecular analysis of the flaA gene B. bronchiseptica for the purpose of ascertaining whether cats become infected with one or more bacteria strains. B. bronchiseptica was isolated from the respiratory system of 16 out of 35 domestic cats with symptoms of respiratory tract infections. Polymorphism analysis of polymerase chain reaction products of B. bronchiseptica flaA was performed to reveal the possible differences in nucleotide sequences of the flagellin gene. The phylogenetic analysis of nucleotide sequences obtained during PCR indicated that the isolates of bacteria from our own studies are characterised by 100% homology of the analysed fragment of the flaA gene, which suggests maintenance of a single genotype of these microorganisms in the cat population. Moreover, the bacteria revealed full homology with reference strain B. bronchiseptica ATCC 4617, and 99.4% homology with strain B. parapertussis ATCC 15311. This indicates that the PCR optimised for the Bordetella spp. flaA gene, combined with sequencing of amplicons obtained in PCR, is an effective diagnostic method allowing differentiation of Bordetella spp. type microorganisms.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs
    Autorzy:
    Adaszek, L.
    Jankowska, M
    Kalinowski, M.
    Banach, T.
    Wulupek, D.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/30873.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.
    Źródło:
    Polish Journal of Veterinary Sciences; 2013, 16, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Applicability of 2D gel electrophoresis and liquid chromatography in proteomic analysis of urine using mass spectrometry MALDI-TOF
    Autorzy:
    Banach, T.
    Adaszek, L.
    Wylupek, D.
    Winiarczyk, M.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/32139.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Proteomics including the studies of the structure, function and dependences between proteins is more and more extensively applied in human medicine and veterinary medicine. The analysis of protein profiles of tissues and body fluid from healthy and ill individuals allows to identify diagnostic, prognostic and predictive markers in various pathological states in people and animals. This paper presents preparation of urine samples for analysis in the mass spectrometer MALDI-TOF (Ultraflextreme, Bruker, Bremen, Germany) by means of two methods: liquid chromatography based on the system Nano-LC (PROTEINER FC II, Bruker Daltonics, Bremen Germany). and two-direction electrophoresis 2DE (GE Healthcare, United Kingdom). Both methods enable separation of the mixture under consideration into individual fractions of high purity indispensable for obtaining readable mass spectra. The purpose of this paper is to determine applicability of these methods in analysis of protein composition of urine samples.
    Źródło:
    Polish Journal of Veterinary Sciences; 2013, 16, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Babesia gibsoni infection in dogs in Poland
    Autorzy:
    Teodorowski, O.
    Kalinowski, M.
    Skrzypczak, M.
    Witt, K.
    Madany, J.
    Winiarczyk, S.
    Adaszek, Ł.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087286.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Babesia gibsoni
    dogs
    PCR
    vector-borne diseases
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 3; 469-471
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cardiac disorders in dogs infected with Babesia canis
    Autorzy:
    Bartnicki, M.
    Buczek, K.
    Staniec, M.
    Adaszek, Ł.
    Winiarczyk, S.
    Łyp, P.
    Dębiak, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087898.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 3; 573-581
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Clinical course of granulocytic anaplasmosis in hunting dogs
    Autorzy:
    Teodorowski, O.
    Winiarczyk, S.
    Debiak, P.
    Skrzypczak, M.
    Mazurek, Ł.
    Adaszek, Ł.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087166.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Anaplasma phagocytophilum
    hunting dog
    vector-borne disease
    Źródło:
    Polish Journal of Veterinary Sciences; 2021, 24, 2; 175-181
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of the bovine viral diarrhoea virus (BVDV) in young beef cattle in eastern and south-eastern regions of Poland
    Autorzy:
    Wernicki, A.
    Urban-Chmiel, R.
    Stegierska, D.
    Adaszek, L.
    Kalinowski, M.
    Puchalski, A.
    Dec, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/30337.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    In view of the scarcity of information concerning viral diarrhoea virus (BVDV) infections in beef cattle in Poland, the aim of this study was to evaluate the presence of the BVDV in young beef cattle from selected herds in eastern and south-eastern regions of Poland. The material consisted of 78 sera obtained from beef cattle from 15 farms, aged 6-12 months. The anti-BVDV antibody level in the sera was estimated with an ELISA kit, and detection of the BVDV was carried out by standard PCR and one step Real-Time RT-PCR. The ELISA results showed a high degree (80%) of positivity in 5 of the 78 samples. In 7 samples the degree of positivity was in the very low range: <40%. Of the 78 cDNA samples, the presence of genetic material with a length of 288 bp was found by standard PCR in 3 sera. The genetic material of BVDV was also found in the sera of the same three calves by Real-Time HRM PCR. BVDV infection in young beef cattle in south-eastern Poland is not a significant problem. This was confirmed by the positive ELISA results for 6.4% of the animals and the positive PCR results for 3.9%. The percentage of positive beef herds was about 8.6%. However, due to the severe nature of the disease and rapid transmission of the virus, regular monitoring of BVDV should be carried out.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough
    Autorzy:
    Kalinowski, M.
    Adaszek, L.
    Miloszowska, P.
    Skrzypczak, M.
    Zietek-Barszcz, A.
    Kutrzuba, J.
    Gradzki, Z.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/31384.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    molecular analysis
    gene
    canine adenovirus 2
    isolation
    dog
    disease symptom
    cough
    upper respiratory tract infection
    Opis:
    The aim of this study was to perform molecular analysis of canine adenovirus 2 (CAV-2) E1B 19K gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the Lubelszczyzna region. Nasal swabs were taken from dogs. DNA of CAV-2 was detected using the PCR method in 16 swabs. All PCR products were sequenced, and the obtained sequences were compared with each other and with the sequence of the E1B 19K gene of the CAV-2 strain from an online database of NCBI GenBank: AC 000003. Based on analysis of the obtained sequences, three polymorphic variants of CAV-2 (No.1-3) with homology of 78 - 100% were distinguished. The nucleotide and amino acid sequences of the most frequently represented polymorphic variant, No. 1, differed from the sequences of polymorphic variant No. 2 with one substitution. The nucleotide and amino acid sequence of the E1B 19K gene of CAV-2 AC 000003 differed from the analogous sequences of representatives of variant No. 1 with 44 nucleotide and 19 amino acid substitutions. The small number of nucleotide differences in the E1B 19K CAV-2 gene among the examined own isolates, compared with AC 000003, suggest that the infections in dogs were caused by a relatively genetically stable virus which occurs in eastern Poland.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on establishing normal ranges of chosen biochemical parameters of haemolymph of Cornu aspersum maxima and Cepaea nemoralis gastropods
    Autorzy:
    Ziętek, J.
    Guz, L.
    Winiarczyk, S.
    Szkucik, K.
    Ziomek, M.
    Wysokowski, M.
    Madany, J.
    Adaszek, Ł.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087699.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Cornu aspersum maxima
    Cepaea nemoralis
    biochemical analysis
    haemolymph
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 445-449
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Diagnosis of the Encephalitozoon cuniculi infections in pet rabbits with neurological symptoms
    Autorzy:
    Zietek, J.
    Adaszek, L.
    Dziegiel, B.
    Kalinowski, M.
    Bartnicki, M.
    Kalinowska, A.
    Jarosz, L.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087942.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The purpose of the study was the in vivo diagnosing of E. cuniculi invasions in pet rabbits with neurological symptoms using the Real-Time PCR, and determination of the rate of invasion, in this group of animals. The study involved 103 pet rabbits with neurological symptoms. Parasitic invasions were diagnosed using Real-Time PCR. The DNA of the parasites for molecular tests was isolated from the urine of the diseased animals. Out of the 103 tested DNA samples, the presence of the E. cuniculi genetic material was detected in 27 samples (26.21%). The melting temperature (Tm) of all products was 77.5℃. The presence of parasitic DNA in the urine of 26.21% of examined animals indicates that E. cuniculi infections occur widely in pet rabbits in Poland and are a significant cause of neurological disorders in those animals.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 2; 361-363
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of coagulase-negative staphylococci isolated from milk of cows with subclinical mastitis
    Autorzy:
    Banach, T.
    Bocniarz, M.
    Lyp, P.
    Adaszek, L.
    Wawron, W.
    Furmaga, B.
    Skrzypczak, M.
    Zietek, J.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/31177.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of this study was to use matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of coagulase-negative staphylococci (CNS) isolated from the milk of cows with subclinical mastitis. The study material consisted of 33 isolates of CNS, identified by the results of API Staph tests, obtained from the milk of cows with subclinical mastitis. Based on the spectra analyses, MALDI-TOF MS tests of 33 bacterial samples allowed identification of the microorganisms in 27 cases (81.8%). The most frequent cause of subclinical mastitis was found to be Staphylococcus sciuri (39%), while S. vitulinus was detected in 15% of the milk samples. The results obtained indicate that MALDI-TOF MS can be used for the identification of CNS isolated from bovine mastitis as a method supplementary to biochemical tests.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-15 z 15

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