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Wyświetlanie 1-15 z 38
Tytuł:
Prevalence of lumbosacral transitional vertebrae in dogs in Berlin
Autorzy:
Gong, H.
Slunsky, P.
Klass, L.G.
Brunnberg, L.
Powiązania:
https://bibliotekanauki.pl/articles/2087324.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
radiology
congenital disease
breed health
hip dysplasia
cauda equina
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 261-265
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of pathogenicity island ETT2 in Escherichia coli isolated from piglets with diarrhea in northeast of China
Autorzy:
Yuan, C.W.
Liu, W.X.
Hou, J.L.
Zhang, L.G.
Wang, G.Q.
Powiązania:
https://bibliotekanauki.pl/articles/2087783.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Escherichia coli
pathogenicity island
ETT2
piglets
diarrhea
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 5-12
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of gastrointestinal trichostrongylidosis on ram fertility
Autorzy:
Gaglio, G.
Poglayen, G.
Capelli, G.
Gruner, L.
Mara, L.
Giannetto, S.
Scala, A.
Powiązania:
https://bibliotekanauki.pl/articles/32463.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
parasite
gastrointestinal trichostrongylidosis
ram
fertility
trichostrongylidosis
Sarda breed
animal infection
Haemonchus contortus
Trichostrongylus colubriformis
Teladorsagia circumcincta
sheep
Opis:
A study on the influence of gastrointestinal trichostrongyles on ram fertility was performed. Two groups of semen donor rams (Sarda breed) were utilized; one was experimentally infected with Haemonchus contortus, Trichostrongylus colubriformis and Teladorsagia circumcincta and the other was used as a control group. In all animals, coprological, haematological and reproductive parameters were studied. The results suggest that the parasites had a limited effect with some changes in phosphorus, cholesterol and chlorine levels. In our experience the parasitic burden produced no significant change on ram fertility.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of goose parvovirus whole VP3 protein and its epitopes in Escherichia coli cells
Autorzy:
Tarasiuk, K.
Wozniakowski, G.
Holec-Gasior, L.
Powiązania:
https://bibliotekanauki.pl/articles/32169.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was the expression of goose parvovirus capsid protein (VP3) and its epitopes in Escherichia coli cells. Expression of the whole VP3 protein provided an insufficient amount of protein. In contrast, the expression of two VP3 epitopes (VP3ep4, VP3ep6) in E. coli, resulted in very high expression levels. This may suggest that smaller parts of the GPV antigenic determinants are more efficiently expressed than the complete VP3 gene.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in physiological and immunological parameters during the peripartum period in Zerasca sheep
Autorzy:
Giuliotti, L.
Lai, O.
Cavallina, R.
Alfieri, L.
Rabusca, G.
Benvenuti, M.N.
Powiązania:
https://bibliotekanauki.pl/articles/32191.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to provide the picture of the dynamics of some blood parameters in the native Zerasca sheep breed during the peripartum period. Blood samples were collected from peripheral blood of 14 ewes at different times: from 21 d before lambing to 42 d after. Physiological and immunological parameters were evaluated: aspartate-aminotransferase, creatine kinase, lactate dehydrogenase, non-esterified fatty acids, total protein, total antioxidant capacity, free radicals, serum bactericidal activity and serum lysozyme. One-way repeated measures ANOVA test was performed. Results showed a significant influence of the peripartum and the deviation from the normal range on many parameters.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Therapeutic activity of chlorhexidine-poloxamer antiseptic gel on wound healing in rats: a preclinical study
Autorzy:
Babickaite, L.
Grigonis, A.
Ramanauskiene, K.
Matusevicius, A.P.
Zamokas, G.
Daunoras, G.
Ivaskiene, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087789.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
skin
wound
chlorhexidine
poloxamer
healing
gel
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 175-183
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2
Autorzy:
Xia, X.J.
Wang, L.
Cheng, L.K.
Shen, Z.Q.
Li, S.G.
Wang, J.L.
Powiązania:
https://bibliotekanauki.pl/articles/31256.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of two water disinfectants (chloramine T and peracetic acid) on the epidermis and gills of Garra rufa used in human ichthyotherapy
Autorzy:
Sirri, R.
Zaccaroni, A.
Di Biase, A.
Mordenti, O.
Stancampiano, L.
Sarli, G.
Mandrioli, L.
Powiązania:
https://bibliotekanauki.pl/articles/31941.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Doctor fish (Garra rufa) have recently been used for aesthetic purposes and as a medical treatment in patients with psoriasis (ichthyotherapy). For this particular kind of human therapy it is essential to guarantee adequate hygienic conditions for both people and fish. The aim of this study was to test two concentrations of water disinfectants, chloramine T and peracetic acid, on Garra rufa to ascertain possible exposure damage to the epidermis and gills. Fish were exposed to 2 mg/l and 10 mg/l of chloramine T and to 15 μl/l and 45 μl/l of peracetic acid in a 40-minute static bath up to six times a day for one week. The epidermis and gills were checked for histological changes and the number of epidermal mucous cells, club cells and taste buds were quantified; mucous cells were also characterized histochemically to detect alterations in mucin production. No mortality or severe histological changes were found in treated or control fish. Cell count showed a significant increase (p<0.05) in mucous cells (mean 49.1 ± 6.7 vs 37.0 ± 13.1 of controls) in animals treated with peracetic acid independently of the dose. Club cell number showed a significant (p<0.05) decrease in fish treated with 2 mg/l of chloramine T (mean 74.3 ± 15.6) and with 45 μl/l of peracetic acid (mean 78.17 ± 10.5) compared to controls (mean 107.0 ± 19.2). Histochemical evaluation of mucous cells did not reveal changes in mucin type in fish exposed to the two disinfectants. The results suggest a good tolerability of Garra rufa to the two disinfectants at the concentrations tested.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vivo induction of autophagy in splenocytes of C57BL/6 and BALB/c mice infected with ectromelia orthopoxvirus
Autorzy:
Martyniszyn, L.
Szulc-Dabrowska, L.
Boratynska-Jasinska, A.
Badowska-Kozakiewicz, A.M.
Niemialtowski, M.G.
Powiązania:
https://bibliotekanauki.pl/articles/31094.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Autophagy is a self-degradation process of cellular components. It plays both antiviral and pro-viral roles in the life cycle of different viruses and the pathogenesis of different viral diseases. In this study, we evaluated autophagy induction in splenocytes of ectromelia virus (ECTV)-resistant C57BL/6 and ECTV-susceptible BALB/c mice during infection with the Moscow strain of the ectromelia virus (ECTV-MOS). Autophagy was analyzed using the Western blot method by assessing type II microtubule-associated protein 1 (MAP1) light chain 3 (LC3) and Beclin 1 expression levels relative to β-actin. Results indicated an increased ratio of LC3-II to β-actin in splenocytes of C57BL/6 mice only at 7 day post infection (d.p.i.) compared to uninfected animals. LC3-II/β-actin and Beclin 1/β-actin ratios in splenocytes of BALB/c mice increased at 5 d.p.i. and remained high until day 14 and 7 p.i., respectively. We confirmed the formation of autophagosome structures in the spleen of BALB/c mice by transmission electron microscopy (TEM). Moreover, autophagy accompanied necrosis in the splenocytes of infected animals. Results suggest that ECTV-MOS induced autophagy, especially in the spleen of the susceptible mouse strain, may support viral replication and promote cell necrosis.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Indirect enzyme-linked immunosorbent assay method based on Streptococcus agalactiae rSip-Pgk-FbsA fusion protein for detection of bovine mastitis
Autorzy:
Bu, R.-E.
Wang, I.-L.
Wu, J.-H.
Xilin, G.W.
Chen, J.-L.
Wang, H.
Powiązania:
https://bibliotekanauki.pl/articles/30156.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Objective: The aim of this study was to establish a rapid and accurate method for the detection of the Streptococcus agalactiae antibody (SA-Ab) to determine the presence of the bovine mastitis (BM)-causative pathogen. Methods: The multi-subunit fusion protein rSip-Pgk-FbsA was prokaryotically expressed and purified. The triple activities of the membrane surface-associated proteins Sip, phosphoglycerate kinase (Pgk), and fibronectin (FbsA) were used as the diagnostic antigens to establish an indirect enzyme-linked immunosorbent assay (ELISA) method for the detection of SA-Ab in BM. Results: The optimal antigen coating concentration was 2 μg/mL, the optimal serum dilution was 1:160, and the optimal dilution of the enzyme-labeled secondary antibody was 1:6000. The sensitivity, specificity, and repeatability tests showed that the method established in this study had no cross-reaction with antibodies to Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis in the sera. The results of the sensitivity test showed that a positive result could be obtained even if the serum dilution reached 1:12,800, indicating the high sensitivity and good repeatability of the method. The positive coincidence rate of this method was 98.6%, which is higher than that of previous tests established with the Sip or Pgk mono-antigen fusion protein, respectively, demonstrating the relatively higher sensitivity of this newly established method. The detection rate for 389 clinical samples was 46.53%. Conclusions: The indirect ELISA method established in this study could provide a more accurate and reliable serological method for the rapid detection of S. agalactiae in cases of BM.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Primordial germ cells ( PGCs) as a tool for creating transgenic chickens
Autorzy:
Chojnacka-Puchta, L.
Kasperczyk, K.
Plucienniczak, G.
Sawicka, D.
Bednarczyk, M.
Powiązania:
https://bibliotekanauki.pl/articles/32300.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
primordial germ cell
transgenic animal
chicken
transgene expression
bioreactor
ovalbumin
Opis:
The transgenic chicken has great potential as a bioreactor for the production of valuable pharmaceutical proteins, notably in the oviduct/egg. Whereas conventional transgenic approaches have significant limitations in this species, an alternative approach employing primordial germ cells (PGCs), the progenitor cells to ova and spermatozoa, has now been successfully applied to the insertion of exogenous genes into birds. Recent developments in manipulating avian embryos make it possible to produce germline chimeras derived from transferred PGCs. In this review we describe the migration pathway of chicken PGCs during early development. We then summarize different methods for the isolation of PGCs and the diversity of techniques used to introduce genes into these cells. Finally, we describe an in vitro assay for testing tissue-specific vectors designed to express heterologous proteins in transgenic chickens.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficacy of topical therapy with newly developed terbinafine and econazole formulations in the treatment of dermatophytosis in cats
Autorzy:
Iwaskiene, M.
Matusevicius, A.P.
Grigonis, A.
Zamokas, G.
Babickaite, L.
Powiązania:
https://bibliotekanauki.pl/articles/31261.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In the field of veterinary dermatology dermatophytosis is one of the most frequently occurring infectious diseases, therefore its treatment should be effective, convenient, safe and inexpensive. The aim of this study was to evaluate the efficacy of newly developed topical formulations in the treatment of cats with dermatophytosis. Evaluation of clinical efficacy and safety of terbinafine and econazole formulations administered topically twice a day was performed in 40 cats. Cats, suffering from the most widely spread Microsporum canis-induced dermatophytosis and treated with terbinafine hydrochloride 1% cream, recovered within 20.3±0.88 days; whereas when treated with econazole nitrate 1% cream, they recovered within 28.4±1.14 days. A positive therapeutic effect was yielded by combined treatment with local application of creams and whole coat spray with enilconazole 0.2% emulsion „Imaverol”. Most cats treated with econazole cream revealed redness and irritation of the skin at the site of application. This study demonstrates that terbinafine tended to have superior clinical efficacy (p<0.001) in the treatment of dermatophytosis in cats compared to the azole tested.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Iron status in dogs with myxomatous mitral valve disease
Autorzy:
Savarese, A.
Probo, M.
Locatelli, C.
Gazzonis, A.L.
Zanzani, S.A.
Traini, G.
Vitiello, T.
Brambilla, P.G.
Powiązania:
https://bibliotekanauki.pl/articles/2087685.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
iron
dog
myxomatous mitral valve disease
cardiorenal syndrome
cardiovascular-renal disorder
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 507-515
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
Autorzy:
Hu, L.
Lin, X.Y.
Yang, Z.X.
Yao, X.P.
Li, G.L.
Peng, S.Z.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/30249.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses
Autorzy:
Gu, X.L.
Dai, F.Y.
Xiao, X.
Li, G.Z.
Zhang, L.M.
Qu, W.J.
Powiązania:
https://bibliotekanauki.pl/articles/2087129.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Ageratina adenophora
liver
lung
horse
toxicity
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 611-613
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 38

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