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Wyświetlanie 1-15 z 62
Tytuł:
Calcium (Ca2+) expression and intensity in cumulus-oocyte complex (COCs) in Kacang goat after vitrification
Autorzy:
Widjiati, W.
Faizah, Z.
Darsini, N.
Hendrawan, V.F.
Karima, H.N.
Chotimah, C.
Sumitro, S.B.
Yustinasari, L.R.
Kasman, A.A.M.N.
Ntoruru, J.M.
Luqman, E.M.
Powiązania:
https://bibliotekanauki.pl/articles/16539072.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Calcium (Ca2+)
confocal laser scanning microscope
cumulus-oocyte complex (COCs)
food production
Kacang goat
Opis:
The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+ in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+ and the intensity of Ca2+ in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+ was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+ in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+ in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+ between fresh COCs and COCs after vitrification. Ca2+ intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 19-26
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Local effect of progesterone infusion into ovarian artery on activin A and inhibin alpha-subunit secretion during the middle luteal phase in gilts
Autorzy:
Wasowska, B
Stefanczyk-Krzymowska, S.
Powiązania:
https://bibliotekanauki.pl/articles/30684.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
dimeric glycoprotein
activin
inhibin
growth factor-beta
progesterone infusion
ovarian artery
alpha-subunit
secretion
exogenous progesterone
local feedback
gilt
middle luteal phase
Opis:
The present study was undertaken to elucidate whether an increased, but physiological, amount of progesterone (P4) supplied to the porcine corpus luteum affects luteal secretion of activin A and inhibin a-subunit (Inha) in freely moving gilts. On day 9 of the estrous cycle (EC), both ovarian arteries and both ovarian veins of gilts (n=5) were cannulated. Progesterone was infused into the right ovarian arteries in gilts on days 10, 11 and 12 of the EC at a rate adequate to its physiological retrograde transfer found during the middle luteal phase of the EC. The P4 infusion rate was 0.62 μg/min (day 10), 2x0.62 μg/min (day 11) and 3x0.62 μg/min (day 12). The left ovarian arteries were infused with saline (control). Blood samples were collected from both ovarian veins on days 10-12 of the EC before and after P4 or saline infusion. The mean plasma activin A level in the ovarian vein ipsilateral to the P4-infused ovary was higher (PcO.OOOl) on days 10-12 of the EC than this found in the contralateral ovarian vein. The level of activin A in the ovarian vein ipsilataral to the infusion of P4 was higher on days 11 (PcO.Ol) and 12 (P<0.0001) and tended to be higher (P<0.07) on day 10 of the EC than this in contralateral ovarian vein. The level of Inha in the ovarian vein ipsilateral to the P4-infused ovary on days 10-12 of the EC was not significantly different (P>0.05) than this found in the contralateral ovarian vein. The results of the present study indicate that a local elevation of P4 concentration in blood supplying the ovary during the middle luteal phase of the porcine EC affects ovarian secretion of activin A. The effect of P4 on the secretion of activin A suggested the existence of a short regulatory loop of a positive feedback between P4 being retrogradely transferred into the ovary and the secretion of this peptide.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 97-104
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Parascaris and cyathostome nematodes in foals: parasite in transit or real infection?
Autorzy:
Kornas, S.
Cabaret, J.
Nowosad, B.
Powiązania:
https://bibliotekanauki.pl/articles/30446.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
nematode
Parascaris equorum
Cyathostominae
foal
coprophagy
parasite
transit
animal infection
horse
gastrointestinal tract
prevalence
Opis:
Faecal egg counts were performed in 187 foals of a large Polish stud farm between February and September 2007. Eggs of Parascaris equorum were present in faeces of 7% and those of cyathostomins in 13% of the foals aged less than 194 days. Information dealing with age of foals and/or efficiency of ivermectin treatment as well as the nematode parasite prepatent periods, it can be conducted that most of the infections recorded on the basis of faecal egg counts were false-infections in animals up to the age of six months, probably due to the ingestion of infected faeces of their dam or some other horses.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Septic arthritis in adult horses
Autorzy:
Carstanjen, B
Boehart, S.
Cislakova, M.
Powiązania:
https://bibliotekanauki.pl/articles/31248.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
arthritis
septic arthritis
horse
adult horse
animal disease
joint disease
articulation
diagnosis
therapy strategy
treatment
novel option
Opis:
Septic arthritis in horses is a serious disease which can become life-threatening. In case the infection can be eliminated before irreversible joint damage occurs, complete recovery is possible. This article gives an overview of the literature concerning etiology, diagnosis and strategies of therapy in cases of septic arthritis in adult horses, with special reference to novel options of treatment.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 201-212
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pituitary adenylate cyclase-activating peptide-27 (PACAP-27) is co-stored with galanin, substance P and corticotropin releasing factor (CRF) in intrapancreatic ganglia of the sheep
Autorzy:
Arciszewski, M.B.
Mozel, S.
Sienkiewicz, W.
Powiązania:
https://bibliotekanauki.pl/articles/31633.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide existing in two variant forms (of either 27 or 38 residues), widely present in numerous organs and evoking multiple effects both in the central and peripheral nervous systems. The present study was undertaken to evaluate the distribution pattern of PACAP-27 expression in the ovine pancreas. Using double immunohistochemical stainings co-localizations of PACAP-27 with galanin, SP or CRF were studied in intrapancreatic neurons. In intrapancreatic ganglia, immunoreactivty to PACAP-27 was found in 87.6 ± 5.4% of PGP 9.5-positive intrapancreatic neurons but not in intraganglionic nerve fibres. Numerous PACAP-27-im-munoreactive nerve terminals were also observed between pancreatic acini and around small arterioles. No immunoreactivity to PACAP-27 was found in the endocrine pancreas. In 42.9 ± 6.2% of PACAP-27-immunoreactive intrapancreatic neurons the expression of galanin was also found. Statistically lower subpopulation (12.4 ± 4.0%) of intrapancreatic neurons exhibited simultaneously the immunoreactivity to PACAP-27 and SP. The expression of CRF was detected in the relatively smallest group (3.2 ± 1.4%) of PACAP-27-positive intrapancreatic neurons. The present results suggest that in the ovine pancreas PACAP-27 may play an important role as mediator of pancreatic functions. In PACAP-related pancreatic activities, a modulatory role of galanin, SP and to a lower extend of CRF is also likely.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of new 18S rRNA strains of Babesia canis isolated from dogs with subclinical babesiosis
Autorzy:
Lyp, P.
Adaszek, L.
Furmaga, B.
Winiarczyk, S.
Powiązania:
https://bibliotekanauki.pl/articles/32165.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In this study, we used PCR to detect and characterize B. canis from naturally infected dogs in Poland with subclinical babesiosis by amplifying and sequencing a portion of the 18S ribosomal RNA (rRNA) gene. Venous blood samples were collected from ten dogs with subclinical babesiosis. A 559-bp fragment of the B. canis 18S rRNA gene was amplified by PCR. Sequencing of the PCR products led to the identification of a new variant of Babesia canis, differing from the previously detected protozoa genotypes (18S rRNA-A and 18S rRNA-B) with nucleotide substitutions in positions 150 and 151 of the tested gene fragment. The results indicate the emergence within the Polish territory of a new, previously unencountered Babesia canis genotype responsible for the development of subclinical babesiosis.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of steroids on noradrenaline-mediated contractile reactivity of the superficial nasal and facial veins in cycling gilts
Autorzy:
Grzegorzewski, W.J.
Muszak, J.
Wasowska, B.
Jana, B.
Stefanczyk-Krzymowska, S.
Powiązania:
https://bibliotekanauki.pl/articles/31116.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The nasal venous blood may be directed through the facial vein into the systemic circulation or through the frontal vein into the venous cavernous sinus of the perihypophyseal vascular complex, where hormones and pheromones permeate from the venous blood into the arterial blood supplying the brain and hypophysis. The present study was designed to determine the effect of noradrenaline (NA) on the tension of the nasal, frontal and facial veins of cycling gilts, and influence of ovarian steroid hormones on NA-mediated contractile reactivity. Additionally, the enzyme dopamine-β-hydroxylase catalysing the conversion of dopamine to noradrenaline (DβH) was immunolocalized in these vessels. Among three studied veins, the frontal proximal vein, that fulfill a key role in the supply of the nasal venous blood into the venous cavernous sinus, reacted to NA most strongly (P<0.001) and this reaction was weaker in the periestrous period than in luteal phase (P<0.001). Inversely, the reaction to NA of the facial proximal vein, that carry blood to the peripheral circulation, was stronger in the periestrous period than in luteal phase (P<0.05). P4, E2 and T significantly lowered NA-mediated tension of the frontal proximal vein during the periestrous period (P<0.001), while in the luteal phase P4 might antagonize relaxing effect of E2 to this vessel. The result suggests that supply of the nasal venous blood into the venous cavernous sinus is greater during the periestrous period than during the luteal phase. DβH was clearly expressed in the muscular layer of the isolated superficial nasal and facial veins of gilts in both studied stages of the estrous cycle. We suggest that the reactivity of the superficial veins of the nose and face to NA combined with the previously demonstrated reactivity of these veins to steroid ovarian hormones and male steroid pheromones may regulate the access of priming pheromone androstenol (resorebed in the nasal cavity) to the brain of gilts during periestrous period via humoral local destination transfer.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity of resveratrol on the influence of aflatoxin B1 on the testes of Sprague dawley rats
Autorzy:
Omur, A.D.
Yildirim, B.
Saglam, Y.S.
Comakli, S.
Ozkaraca, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087503.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
aflatoxin B1
rat
resveratrol
sperm
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 313-320
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunocytochemical detection of calretinin in the claustrum and endopiriform nucleus of the chinchilla
Autorzy:
Szalak, R.
Matysek, M.
Mozel, S.
Arciszewsk, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/30835.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Calretinin (CR) as a buffer and sensor protein plays an important role in regulatory processes of Ca²⁺ and anty-apoptotic cellular protection. In the present study, immunohistochemical peroxidase- antiperoxidase (PAP) method was used in order to determine the numbers, morphology, morphometry and distribution pattern of CR in neurons of the chinchilla’s claustrum (Cl) and endopiriform nucleus (EN). In Cl and EN the presence of several classes of neurons with different immunoreactivity to CR was found. In Cl, CR-immunoreactive (IR) neurons were predominantly found in close vicinity to insular border while CR-IR neurons were evenly scattered throughout EN. In general, immunoreaction to CR was observed in neuronal cytoplasm, nucleus and in fibres-like nerve extensions. Statistical analysis showed the differences between average large diameter as well as cross-sectional area of CR-IR neurons present in Cl and EN. It is suggested, that CR acting as a calcium binding protein may play a role in neuronal network. Further co-localization studies are necessary to fully elucidate neurophysiology and neuropathology of the chinchilla’s Cl and EN neurons.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of heat stress on fattening performance, carcass traits, oxidant/antioxidant status, and hepatic heat shock protein 70 levels in different plumage colors of Japanese quail (Coturnix coturnix japonica)
Autorzy:
Aslan, S.
Baykalir, Y.
Simsek, U.G.
Gul, B.
Powiązania:
https://bibliotekanauki.pl/articles/16647382.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
biochemistry
carcass yield
heat stress
quail
Opis:
The aim of this study was to investigate the impact of heat stress on production performance and oxidative stress in different plumage colors of Japanese quail. For this purpose, a total of 100 birds were used in this study. The 25 birds belonged to Wild-type (n=25, grey), Tuxedo (n=25, black), Golden (n=25, yellow) and Recessive white (n=25). The birds were reared for 42 days in an environmentally controlled room at 39°C and relative humidity of 60-65%. The body weight, body weight gain (g/bird/day), and feed conversion ratio were not different between the groups (p>0.05). However, the feed intake (g/bird/day) of the Wild-type had a higher value than the Tuxedo (black) group counterparts between 15 and 21 days different (p<0.05). There was no significant effect of heat stress on the carcass traits (p>0.05). Spleen weights were different between the groups (p<0.05). The yellow group had the highest spleen weight. The highest MDA level was found in the Recessive White variety, followed by Wild-type (grey), Golden (yellow) and Tuxedo (black), respectively. However, there were no statistical differences amongst the groups (p>0.05). There was also no statistical significance in glutathione (GSH) and superoxide dismutase (SOD) levels (p>0.05). The heat shock protein 70 kDa (HSP70) level was significantly different between the groups (p<0.001). The highest percentage was observed in the Golden (5.06%) and the lowest in the White (1.43%) variety. There was no superior color variety of Japanese quail regarding fattening performance and carcass traits. It is conceivable that when considering the stress response of the different colors, the Golden group is more sensitive to stress due to the hepatic and cellular level of HSP70.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 599-605
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of microflora composition on safety and colour parameters of “kumpia wieprzowa” during ripening
Autorzy:
Wesierska, E.
Korzekwa, K.
Foks, S.
Mickowska, B.
Powiązania:
https://bibliotekanauki.pl/articles/30981.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to define the influence of microbiological activity on the safety (microflora composition, biogenic amine amount) and colour of “kumpia wieprzowa” during the 3-month ripening period. The study included the amount of aerobic bacteria, yeast, lactobacilli rods, coagulase- negative cocci, pH and colour parameters as well as the content of nitrates (V) and (III), biogenic amines and amino acids. The lactobacilli and cocci constituted the predominant microflora of the ready-to-eat product (4.9-5.2 and 5.2-5.4 log cfu/g, respectively), although further mesophilic bacteria identification revealed the presence of numerous aerobic, aerotolerant and anaerobic species, mostly gram-positive, spore- and non-spore-forming. The absence of 2-phenylethylamine and putrescine and the low level of tryptamine (2.5 mg/kg) at the beginning of the ripening as well as the increase of tyramine and spermine amounts from 11.5 and 2.7 to 21.9 and 4.0 mg/kg, respectively during the treatment, denoted the good quality of raw meat used and dynamic growth of the desired acidifying and denitrifying microorganisms. The development of the coagulase-negative cocci population corresponded with the a* and C* colour parameters and the nitrate (III) content increase, the final result of which was 26.9, 27.5 as well as 19.4 mg/kg. The content of nitrates (V) and (III) was optimal to obtain a non-cured, safe and suitably coloured, long-term ripened meat product.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of self-biting behavior of mink by loop-mediated isothermal amplification (LAMP) and sequence-characterized amplified regions (SCAR)
Autorzy:
Liu, Z.Y.
Song, S.S.
Huo, Z.S.
Song, X.C.
Cong, B.
Yang, F.H.
Powiązania:
https://bibliotekanauki.pl/articles/2087715.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mink
self-biting
loop-mediated isothermal amplification
sequence-characterized amplified regions
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 371-376
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synergistic interactions between resveratrol and doxorubicin inhibit angiogenesis both in vitro and in vivo
Autorzy:
Uvez, A.
Aydinlik, S.
Esener, O.B.B.
Erkisa, M.
Karakus, D.
Armutak, E.I.
Powiązania:
https://bibliotekanauki.pl/articles/2087274.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
resveratrol
chick chorioallantoic membrane (CAM) assay
doxorubicin
HUVEC
tube formation
anti-angiogenic activity
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 4; 571-580
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of acute lipopolysaccharide-induced toxemia model on some neglected blood parameters
Autorzy:
Adam, G.O.
Lee, H.R.
Lee, S.J.
Kim, S.J.
Kim, G.B.
Kang, H.S.
Powiązania:
https://bibliotekanauki.pl/articles/2087627.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Toxemia
lipopolysaccharide
base excess
pH
animal model
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 4; 665-671
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical study on the expression of biologically active substances in the endocrine pancreas of the European bison
Autorzy:
Mozel, S.
Szymańczyk, S.
Krzysiak, M.
Puzio, I.
Zacharko-Siembida, A.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/2087293.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
glucagon-like peptide-1
glucagon
insulin
somatostatin
immunohistochemistry
pancreas
European bison
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 333-340
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 62

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