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Wyszukujesz frazę "Yang, huang" wg kryterium: Wszystkie pola

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    Wyświetlanie 1-7 z 7
    Tytuł:
    Pharmacokinetics of diclofenac sodium injection in swine
    Autorzy:
    Yang, H.F.
    Li, Y.J.
    Li, Y.Y.
    Huang, C.
    Huang, L.X.
    Bu, S.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087550.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    diclofenac sodium injection
    pharmacokinetics
    bioavailability
    swine
    HPLC
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 423-426
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
    Autorzy:
    Han, K.
    Zhao, D.
    Liu, Y.
    Liu, Q.
    Huang, X.
    Yang, J.
    Bi, K.
    Xu, T.
    Li, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/31495.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Construction of a recombinant baculovirus expressing swine hepatitis E Virus ORF2 and preliminary research on its immune effect
    Autorzy:
    Yang, Z.
    Wang, K.
    Hu, Y.
    Yuan, P.
    Yang, Y.
    Xie, L.Y.
    Huang, S.L.
    Liu, J.
    Ran, L.
    Song, Z.H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087759.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    swine HEV
    ORF2
    baculovirus
    immunogenicity
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 47-54
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of Neuromedin S on the proliferation of splenic lymphocytes and the cytokine secretion by pulmonary alveolar macrophages in pigs in vitro
    Autorzy:
    Lin, R.
    Wang, Q.
    Qi, B.
    Huang, Y.
    Yang, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/30295.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Neuromedin S (NMS), a 36-amino acid neuropeptide, has been found to be involved in the regulation of the endocrine activity. It has been also detected in immune tissues in mammals, what suggests that NMS may play an important role in the regulation of immune response. The aim of this study was to demonstrate the presence of NMS receptor 1 (NMU1R) and effect of NMS in pig splenic lymphocytes (SPLs) and pulmonary alveolar macrophages (PAMs). The presence of NMU1R in pig SPLs and PAMs was respectively confirmed by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis and immunocytochemical methods. Furthermore, SPL proliferation was analyzed using the 3-(4,5)-dimethyl-thiahiazo-(-2-yl)-3,5-di-phenytetrazoliumromide (MTT) method. Additionally, the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in PAMs was all measured by enzyme-linked immunosorbent assay (ELISA) kits. In the present study, the results of RT-PCR and western blot analysis revealed that NMU1R mRNA and protein were both expressed in pig SPLs and PAMs, and the immunocytochemical investigations further revealed that the positive signal of NMU1R immunoreactivity was observed in plasma membranes of both SPLs and PAMs. In the in vitro study, we found that at concentrations of 0.001-1000 nM NMS alone or combined with lipopolysaccharide or phytohemagglutinin significantly increased SPL proliferation. Application of ELISA method showed that NMS could induce the secretion of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in PAMs. These results suggest that NMS can act as a potently positive pro-inflammatory factor and immunomodulatory agent that affects the immune response of immune cells by combining with its receptor NMU1R.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Caspase-1 inhibitor reduced the lung injury in a mouse model of pleuropneumonia caused by Actinobacillus pleuropneumoniae
    Autorzy:
    Zhang, Y.
    Yang, T.
    Huang, F.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087250.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    porcine contagious pleuropneumonia
    mice
    caspase-1 inhibitors
    caspase-1dependent pyroptosis
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 4; 605-610
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
    Autorzy:
    Dai, G.
    Huang, X.
    Liu, Q.
    Li, Y.
    Zhang, L.
    Han, K.
    Yang, J.
    Liu, Y.
    Xue, F.
    Zhao, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647377.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    epitope
    goose astrovirus
    capsid protein
    monoclonal antibody
    Opis:
    Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
    Autorzy:
    Xu, T.
    Huang, X.
    Li, Y.
    Liu, Q.
    Liu, X.
    Han, K.
    Liu, Y.
    Yang, J.
    Zhao, D.
    Bi, K.
    Sun, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087719.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    goose-derived
    duck-derived
    chicken-derived
    Tembusu virus
    pathogenicity
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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