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Wyszukujesz frazę "Wojcik, J" wg kryterium: Wszystkie pola


Wyświetlanie 1-10 z 10
Tytuł:
Transport induced inflammatory responses in horses
Autorzy:
Wessely-Szponder, J.
Belkot, Z.
Bobowiec, R.
Kosior-Korzecka, U.
Wojcik, M.
Powiązania:
https://bibliotekanauki.pl/articles/30538.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Deleterious response to road transport is an important problem in equine practice. It determines different physiological, immunological and metabolic changes which lead to increased susceptibility to several disorders such as pneumonia, diarrhea, colics, laminitis, injuries and rhabdomyolisis. The aim of our study was to look for possible relationships between transportation of female young and older horses over a long and short distance and an inflammatory state reflected by an increase of acute phase protein concentration, oxidative stress and muscle injury. The study was conducted on 24 cold-blooded female horses divided into four groups. Six fillies aged 6-18 months and six mares aged 10-12 years were transported over the distance of about 550 km, six fillies aged 6-18 months and six mares aged 10-12 years were transported over the distance of about 50 km. Plasma and serum were obtained from blood samples taken before transportation (T0), immediately after transportation (T1) and at an abattoir during slaughter (T2). In these samples fibrinogen, MDA, AST and CK were assessed. Fibrinogen increased in all studied groups especially in fillies after long distance transportation, where it reached 205±7.07 mg/dl before transportation, 625±35.35 mg/dl after transportation, and 790±14.14 mg/dl during slaughter. MDA concentrations rose after transportation and reached the maximal level during slaughter. CK activity was more elevated after short transportation in younger horses, whereas initial activity of AST was higher in older horses. We estimated that intensified responses from acute phase, oxidative stress and muscle injury parameters indicated an inflammatory state.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of beta-hydroxy-beta-methylbutyrate (HMB) on selected parameters of humoral immunity in calves
Autorzy:
Wojcik, R.
Malaczewska, J.
Siwicki, A.K.
Micinski, J.
Zwierzchowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/2087958.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of this study was to evaluate the effect of HMB on selected parameters of the humoral immunity in calves. The experiment was performed on 14 calves aged 30 ± 2 days, divided into two equal groups of control (group K) and experimental (group H) animals. The feed administered to the experimental calves was supplemented with HMB at 40 mg/kg BW, whereas the control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following immunological parameters: total protein levels, gammaglobulin levels, lysozyme activity and ceruloplasmin activity. An analysis of the results obtained revealed a significant increase (p < 0.05; p < 0.01; p < 0.001 respectively) in gammaglobulin levels and lysozyme activity throughout the entire experimental period, an increase (p < 0.05; p < 0.01 respectively) in ceruloplasmin activity on experimental days 15 and 30, but no changes in serum total protein levels of calves administered HMB as compared to those found in the control group.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 357-359
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proliferative and oxidative response of hepatocytes (Hep) and hepatic stellate cells (HSC) isolated from rats exposed to ketogenic diet
Autorzy:
Wojcik, M.
Wessely-Szponder, J.
Kosior-Korzecka, U.
Powiązania:
https://bibliotekanauki.pl/articles/30839.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Ketogenic diet (KD) is considered in the context of its anti-epileptic effects, but its influence on liver dysfunction has not been elucidated yet. The study was aimed to investigate the activity of hepatocytes (Hep) and hepatic stellate cells (HSC) isolated from rats fed with KD, in respect of NO and superoxide generation by these cells as well as their proliferative activity in vitro. We also sought to characterize the plasma FFA profiles in control and ketogenic rats. Hep and HSC were isolated by the collagenase perfusion method and separated by the Percoll gradient centrifugation. After the 4th, 8th and 12th day of incubation, the media were collected for further analysis. NO generation increased within the time of incubation both in Hep and HSC isolated from KD-rats. In HSC group NO production raised significantly from 2.65 ± 0.07 μM/10⁶ cells on 4th day of incubation to 5.49 ± 1.2 μM/10⁶ cells on 12th day of incubation. In respect to O₂-. generation experimental Hep and HSC provide considerably higher quantities of this free radical. until 12th day of incubation (2.5 ± 0.07 and 3.2 ± 0.3 nM/10⁶ cells, respectively). Although KD exerts anti-proliferative effect on hepatocytes, in respect to HSC it intensifies their proliferative activity. Furthermore, as we estimated on the basis of NO and O₂-. generation both Hep and HSC exposed to KD are the source of free radicals.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of beta-hydroxy-beta-methylbutyrate (HMB) on the proliferative response of blood lymphocytes and the phagocytic activity of blood monocytes and granulocytes in calves
Autorzy:
Wojcik, R.
Malaczewska, J.
Siwicki, A.K.
Micinski, J.
Zwierzchowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/32319.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of this study was to evaluate the effect of HMB on selected indicators of immunity in calves. The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following parameters of immunity: proliferative response of LPS- and ConA-stimulated lymphocytes (MTT), respiratory burst activity (RBA) and potential killing activity (PKA) of phagocytes. The results revealed a significant increase in RBA and MTT values in calves administered HMB in comparison with the control group throughout the experiment. In the group of animals receiving HMB, an increase in PKA values was noted only on day 30.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The in vitro effect of kynurenic acid on the rainbow trout (Oncorhynchus mykiss) leukocyte and splenocyte activity
Autorzy:
Malaczewska, J.
Siwicki, A.K.
Wojcik, R.
Turski, W.A.
Kaczorek, E.
Powiązania:
https://bibliotekanauki.pl/articles/32542.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Kynurenic acid (KYNA), an endogenous neuroprotectant formed along the kynurenine pathway of tryptophan degradation, is a selective ligand of the GPR35 receptor, which can be found on the surface of various populations of human immune cells. In infections and inflammations, KYNA produces an anti-inflammatory effect through this receptor, by depressing the synthesis of reactive oxygen species and pro-inflammatory cytokines. However, it is still unrecognized whether receptors for kynurenic acid are also localized on immune cells of poikilothermic animals, or whether KYNA is able to affect these cells. The objective of this study has been to determine the effect of different concentrations of kynurenic acid (12.5 μM to 10 mM) on the viability and mitogenic response of lymphocytes and on the activity of phagocytic cells isolated from blood and the spleen of rainbow trout. The results imply low toxicity of kynurenic acid towards fish immune cells, and the proliferative effect observed at the two lowest concentrations of KYNA (12.5 μM and 25 μM) seems indicative of endogenous kynurenic acid being capable of activating fish lymphocytes. Non-toxic, micromole concentrations of KYNA, however, had no influence on the mitogenic response of lymphocytes nor on the activity of phagocytes in rainbow trout under in vitro conditions. There is some likelihood that such an effect could be observed at lower, nanomole concentrations of KYNA.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characteristics of advanced methods used for typing bacterial isolates from mastitis with particular reference to Staphylococci
Autorzy:
Lisowska-Łysiak, K.
Dudko, P.
Kosecka-Strojek, M.
Walczak, J.
Wójcik, P.
Międzobrodzki, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087754.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
coagulase-negative staphylococci
genetic methods
mastitis
phenotypic methods
Staphylococcus aureus
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 229-239
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The in vitro effect of bovine lactoferrin on the activity of organ leukocytes in rainbow trout [Oncorhynchus mykiss], European eel [Anguilla anguilla] and wels catfish [Silurus glanis]
Autorzy:
Malaczewska, J
Wojcik, M.
Wojcik, R.
Siwicki, A.K.
Powiązania:
https://bibliotekanauki.pl/articles/30969.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fish
rainbow trout
Oncorhynchus mykiss
European eel
Anguilla anguilla
catfish
Silurus glanis
in vitro effect
bovine lactoferrin
lactoferrin
leucocyte
freshwater fish
Opis:
Lactoferrin (LF) is a glycoprotein found in milk, neutrophil granules, secretions and selected organs of mammals. Lactoferrin exhibits antibacterial, antiviral, fungicidal, immunoregulatory and other functions. Although fish are devoid of this protein and its cell receptors, LF effect on the immune mechanisms of fish has been demonstrated. The objective of this study was to investigate the effect of bovine lactoferrin, applied in vitro, on the activity of head kidney and spleen leukocytes in three freshwater fish species: rainbow trout (Oncorhynchus mykiss), European eel (Anguilla anguilla) and weis catfish (Silurus glanis). The obtained results validate LF beneficial effect on the respiratory burst of phagocytes in rainbow trout and weis catfish despite the fact that the potential killing activity against Aeromonas hydrophila was not stimulated in any of the studied species. Bovine lactoferrin enhanced the proliferation of T-lymphocytes in rainbow trout and European eel, as well as of B-lymphocytes in rainbow trout.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 83-88
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The concentration of urea in hemolymph as a marker of health in Lissachatina fulica and Cornu aspersum edible snails – a preliminary study
Autorzy:
Ziętek, J.
Guz, L.
Wójcik, A.
Winiarczyk, S.
Adaszek, Ł.
Powiązania:
https://bibliotekanauki.pl/articles/2087559.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Lissachatina fulica
Cornu aspersum
hemolymph
urea
markers
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 259-262
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of undecanones and their derivatives on tumor angiogenesis and VEGF content
Autorzy:
Gibka, J
Wasiutynski, A.
Skopinska-Rozewska, E.
Siwicki, A.K.
Chorostowska-Wynimko, J.
Sommer, E.
Mazurkiewicz, M.
Glinski, M.
Skurzak, H.
Wojcik, R.
Jung, L.
Powiązania:
https://bibliotekanauki.pl/articles/31734.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mice
mouse
undecan-x-one
derivative
angiogenesis
tumour
VEGF zob.vascular endothelial growth factor
enantiomer
histological examination
vascular endothelial growth factor
sarcoma cell
Opis:
The in vivo effects of some derivatives of aliphatic ketones (2-undecanone, 3-undecanone, 4-undecanone and their derivatives) on L-l sarcoma tumor angiogenesis and VEGF content were studied in Balb/c mice. Mice that inhaled 10% solution of 3-undecanone(3-on) or 1% solution of 2-undecanone propylene acetal (Acpr2) for 3 days after tumor cells implantation, presented lower neovascular response measured by tumor-induced cutaneous angiogenesis test (TIA) and lower tumor VEGF content in 5-days tumors, than non-inhaled controls. Other substances presented various effects on tumor VEGF concentration and angiogenesis. Histological examination of lesions collected from mice inhaled Acpr2, or non-inhaled controls, revealed small diffused areas of necrosis in the former group. In both groups, slight to moderate inflammatory infiltrations were seen at the tumor's margin. In Acpr2 group, there were less small blood vessels at tumor's margin than in the control group.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 105-115
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The applicability of the Migratest® kit for evaluating the chemotactic activity of peripheral blood neutrophils in goats on the example of animals supplemented with β-hydroxy-β-methylbutyrate (HMB)
Autorzy:
Wójcik, R.
Małaczewska, J.
Kaczorek-Łukowska, E.
Powiązania:
https://bibliotekanauki.pl/articles/2087425.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goats
Migratest® kit
chemotactic activity of peripheral blood neutrophils
β-hydroxy-β-methylbutyrate
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 725-733
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-10 z 10

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