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Wyświetlanie 1-15 z 121
Tytuł:
Accuracy of bovine pregnancy-associated glycoproteins (bPAGs) in the diagnosis of pregnancy: A comparative study of three pregnancy diagnostic methods
Autorzy:
Ghaidan, M.T.
Dana, O.I.
Dyary, H.O.
Powiązania:
https://bibliotekanauki.pl/articles/2087383.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bPAGs
detection of pregnancy
progesterone
rectal palpation
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 769-775
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of deoxynivalenol on the levels of toll-like receptors 2 and 9 and their mRNA expression in enterocytes in the porcine large intestine: a preliminary study
Autorzy:
Dabrowski, M.
Jakimiuk, E.
Gajecka, M.
Gajecki, M.T.
Zielonka, L.
Powiązania:
https://bibliotekanauki.pl/articles/32154.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Deoxynivalenol (DON), one of the most prevalent mycotoxins in the world, and is capable of inducing immune disorders in humans and animals. The aim of this study was to determine the effect of feed contaminated with DON on the number of TLR2- and TLR9-positive cells and their mRNA expression in the porcine large intestine. The experiment was conducted on two equal groups of pigs (n=4). The experimental group (E) was administered feed contaminated with DON (1008 μg/kg of feed) for 6 weeks, and the control group (C) was administered non-contaminated feed over the same period of time. A decrease in the expression of TLR2 mRNA was noted in the cecum. The percentage of TLR9-positive enterocytes increased in the ascending colon and decreased in the cecum. The results of this study indicate that DON can modify the local immune response by changing the expression of TLRs.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adrenal gland tumors in dairy cattle from Northern Italy: morphological and phenotypical characterizationin comparison with human pathology
Autorzy:
Biasibetti, E.
Giorcelli, J.
Deideri, F.
Bianco, P.
Capucchio, M.T.
Volante, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087856.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 779-788
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bone marrow-origin stem/progenitor cells in the mammary gland of heifers
Autorzy:
Osinska, E.
Godlewski, M.M.
Wierzbicki, M.
Motyl, T.
Powiązania:
https://bibliotekanauki.pl/articles/30416.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to estimate the size of bone marrow-origin stem/progenitor population in 2-year old nonpregnant Holstein-Friesian heifers. Quantitative and qualitative analysis was done using scanning cytometry and confocal microscopy of mammary tissue slices labelled with the combination of two markers: Sca-1 (marker of stem-progenitor cells) and CD45 (marker of hematopoietic cells). The average (±SEM) percentage of Sca-1POS CD45POS cells was 0.89 ± 0.21. They were localized mainly outside of mammary ducts, in the stroma and sometimes intraluminally. Our results indicate that the subpopulation of Sca-1POS cells bearing CD45 antigen may enrich the niche of mammary stem/progenitor cells from the bone marrow and participate in the growth of the mammary gland in post-pubertal heifers.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenotypic and genotypic antimicrobial resistance of staphylococci from bovine milk
Autorzy:
Kot, B.
Piechota, M.
Wolska, K.M.
Frankowska, A.
Zdunek, E.
Binek, T.
Klopotowska, E.
Antosiewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/31274.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to examine phenotypic and genotypic antimicrobial resistance of staphylococci from milk samples from cows with subclinical and clinical mastitis and from cows without mastitis symptoms to methicillin, tetracyclines, macrolides and lincosamides (ML). Of 207 strains, including 34 S. aureus and 173 coagulase-negative staphylococci (CNS), 11 (6.4%) CNS strains were phenotypically resistant to methicillin. The mecA gene was detected by PCR only in two S. xylosus strains and one strain of S. epidermidis and S. simulans. No methicillin-resistant S. aureus strains were observed. In methicillin-resistant strains with mecA, gene resistance to other investigated antibiotics was not observed. Phenotypic resistance to tetracycline was detected in 11.0% of CNS strains and 47.4% of them carried the tetK gene. Of 173 CNS strains studied, 27 (15.6%) were resistant to at least one ML antibiotic. The resistance gene ermC was detected in 55.5% of the 27 ML-resistant strains. The ermA and ermB genes were detected in 14.8% and 11.1% of ML-resistant CNS strains, respectively. Antimicrobial resistance to methicillin, tetracyclines and macrolides was detected more frequently in staphylococcal strains from clinical mastitis compared to animals with subclinical symptoms and without mastitis, while the resistance to lincosamides showed a similar frequency in all groups of cows. In conclusion, CNS species from bovine milk differ in phenotypic and genotypic antimicrobial resistance profiles, and the use of PCR technique alone for the detection of methicillin, macrolide, lincosamide and tetyracycline resistance in CNS from cattle is not reliable.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prostaglandin E2 inhibits IL-10 production by bovine CD4plus T cells
Autorzy:
Zuska-Prot, M.
Maslanka, T.
Jasiecka, A.
Jaroszewski, J.J.
Powiązania:
https://bibliotekanauki.pl/articles/31834.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Although prostaglandin E₂ (PGE₂) is a pro-inflammatory mediator, it also produces some effect which is anti-inflammatory in character. It is suggested that one of the mechanisms responsible for the latter effect is the increased synthesis of IL-10. The aim of this study has been to determine the influence of PGE₂ on IL-10 production by bovine CD⁴⁺ and CD⁸⁺ T cells and NK cells. With this aim, peripheral blood mononuclear cells collected from 12-month-old heifers (n = 10) were treated without or with PGE₂ (10⁻⁶ M). Flow cytometric analysis showed that PGE₂ caused a reduction in the percentage of IL-10 producing CD⁴⁺ T cells (P < 0.001), while leaving the secretion of this cytokine by CD⁸⁺ T cells and NK cells unaffected. This seems to indicate that PGE₂ in cattle does not produce an anti-inflammatory effect by increasing the synthesis of IL-10; contrary to this, it may aggravate an inflammatory response by inhibiting the secretion of this cytokine by CD⁴⁺ T cells.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The transcriptomic signature of myostatin inhibitory influence on the differentiation of mouse C2C12 myoblasts
Autorzy:
Wicik, Z.
Sadkowski, T.
Jank, M.
Motyl, T.
Powiązania:
https://bibliotekanauki.pl/articles/30247.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
GDF8 (myostatin) is a unique cytokine strongly affecting the skeletal muscle phenotype in human and animals. The aim of the present study was to elucidate the molecular mechanism of myostatin influence on the differentiation of mouse C2C12 myoblasts, using the global-transcriptome analysis with the DNA microarray technique. Treatment with exogenous GDF8 strongly affected the growth and development of C2C12 mouse myoblasts. This was manifested by the inhibition of proliferation and differentiation as well as the impairment of cell fusion. DNA microarray analysis revealed 778 genes regulated by GDF8 in differentiating myoblasts (436 down-regulated and 235 up-regulated). Ontological analysis revealed their involvement in 17 types of biological processes, 10 types of molecular functions and 68 different signalling pathways. The effect of GDF8 was mainly mediated by the disruption of the cell cycle, calcium and insulin signalling pathways and expression of cytoskeletal and muscle specific proteins. The identified key-genes that could play a role as GDF8 targets in differentiating myoblasts are: Mef2, Hgf, Ilb1, Itgb1, Edn1, Ppargc1a.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Malignant thymoma – the most common neoplasm in goats
Autorzy:
Szaluś-Jordanow, O.
Czopowicz, M.
Witkowski, L.
Mickiewicz, M.
Moroz, A.
Kaba, J.
Sapierzyński, R.
Bonecka, J.
Jońska, I.
Garncarz, M.
Kwiatkowska, M.
Chutorański, D.
Szołkowska, M.
Frymus, T.
Powiązania:
https://bibliotekanauki.pl/articles/2087485.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goats
thymus
tumor
neoplasm
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 475-480
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytomorphometry of caine cutaneous histiocytoma
Autorzy:
Pazdzior-Czapula, K.
Otrocka-Domagala, I.
Rotkiewicz, T.
Gesek, M.
Powiązania:
https://bibliotekanauki.pl/articles/31113.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
A morphometric analysis of tumoral Langerhans cells and activated macrophages was conducted using canine cutaneous tumors (65 cases of canine cutaneous histiocytoma and 7 cases of pyogranuloma). The histiocytic origin of the tumor cells was confirmed using immunohistochemistry. The parameters of the morphometric analysis included cellular and nuclear size and shape and the nuclear: cytoplasmic ratio; the variability of these features was calculated separately for each tumor. The canine cutaneous histiocytoma group was divided into four stages of regression depending on the intensity of the lymphocytic infiltration. Statistical analysis revealed that the anisocytosis, anisokaryosis and cellular pleomorphism of tumoral Langerhans cells increased, while the cellular circularity and nuclear:cytoplasmic ratio decreased with tumor regression. Activated macrophages of the pyogranuloma were significantly larger, and had larger nuclei, than tumoral Langerhans cells. Furthermore, these activated macrophages showed greater anisocytosis and anisokaryosis and a lower nuclear: cytoplasmic ratio than tumoral Langerhans cells in the first stages of tumor regression. These results indicate that tumoral Langerhans cells undergo morphologic changes during the regression of canine cutaneous histiocytoma, reflecting their maturation and differentiation. Morphometry can be a useful method for distinguishing activated macrophages from tumoral Langerhans cells.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution of CD4plusCD8plus double positive T cells in a mouse model of allergic asthma
Autorzy:
Zuska-Prot, M.
Ziolkowski, H.
Jaroszewski, J.J.
Maslanka, T.
Powiązania:
https://bibliotekanauki.pl/articles/32502.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The present study describes the distribution of CD4+CD8+ double-positive (DP) T cells in various immune compartments of mice with ovalbumin (OVA)-induced allergic asthma. It was found that the absolute number of DP T cells was considerably increased in the mediastinal lymph nodes and lungs of asthmatic mice as compared with that determined in the healthy subjects. On the contrary, the absolute counts of DP T cells was significantly decreased in the head and neck lymph nodes, and in peripheral blood of OVA-immunized mice. These results suggest that DP T cells may be involved in the pathogenesis of allergic asthma.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Canine mammary carcinoma cell line are resistant to chemosensitizers: verapamil and cyclosporin A
Autorzy:
Krol, M.
Pawlowski, K.M.
Majchrzak, K.
Mucha, J.
Motyl, T.
Powiązania:
https://bibliotekanauki.pl/articles/32199.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Cancer chemotherapy can fail in many ways. One of the most significant is the development of multiple drug resistance (MDR), which constitutes a serious clinical problem. The development of MDR relates to the expression of a major membrane pump, P-glycoprotein (P-gp). Thus, currently one of the goals of experimental and clinical oncology is to decrease its activity. So far, many different P-gp inhibitors are available, but their efficacy is still questionable and requires further study. The aim of our study was to assess an impact of classical P-gp inhibitors (verapamil and cyclosporin A) in the reversion of multidrug resistance in canine mammary cancer cells. We used two cell lines isolated from mammary tumors and two cell lines isolated from their lung metastases. All of them showed P-gp over-expression confirmed using Real-time rt-PCR, Skan^R screening station and confocal microscopy. The FACS analysis showed that in three of the examined cell lines, treatment with verpamil/cyclosporin A was ineffective to reverse cancer chemoresistance. However, more studies in this field are required.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of bestatin, an inhibitor of aminopeptidases, on T and B lymphocyte subsets in mice
Autorzy:
Lis, M.
Szczypka, M.
Suszko, A.
Obminska-Mrukowicz, B.
Powiązania:
https://bibliotekanauki.pl/articles/30949.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Bestatin, a low-molecular weight dipeptide, is a potent inhibitor of aminopeptidase N which has been demonstrated to have antitumor and immunomodulatory effects. The effects of bestatin (10, 1 and 0.1mg/kg) administered intraperitoneally once, five or ten times to mice on the total number of lymphocytes in the thymus, spleen and mesenteric lymph nodes and the percentage and the absolute number of T cell subsets (CD4+CD8+, CD4-CD8-, CD4+, CD8+) in the thymus and T (CD3+, CD4+, CD8+) and B (CD19+) lymphocytes in the spleen and mesenteric lymph nodes were studied. It has been found that bestatin administered ten times at doses of 10, 1 and 0.1 mg/kg increased the total number of thymocytes, splenocytes and lymphocytes of mesenteric lymph nodes. Bestatin also changed the percentage and the absolute number of T cell subsets in the thymus and T and B lymphocytes in the peripheral lymphatic organs. Five and ten exposures to bestatin (10, 1 and 0.1 mg/kg) increased the absolute count of both immature CD4+CD8+ and CD4-CD8- thymic cells. Moreover, both a single and multiple administration of bestatin (1 and 0.1 mg/kg) decreased the percentage and absolute count of CD3+ splenocytes and mesenteric lymph node cells with corresponding decreases in the percentage and absolute count of CD4+ and CD8+ cells. Both a single and multiple administration of bestatin at all the doses under investigation augmented the percentage and the absolute count of CD19+ (B lymphocytes) in the peripheral lymphatic organs. The results of the study show that there is a relationship between the effect induced by bestatin and the dose of the drug as well as the number of doses applied. The strongest effect on the T and B lymphocyte subsets was noted after five injections of bestatin at doses of 1 and 0.1 mg/kg.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The efficiency of synthetic sex pheromones in sexual arousal stimulation in domestic dogs
Autorzy:
Dzięcioł, M.
Niżański, W.
Jezierski, T.
Szumny, A.
Godzińska, E.J.
Ochota, M.
Stańczyk, E.
Najder-Kozdrowska, L.
Woszczyło, M.
Pieczewska, B.
Powiązania:
https://bibliotekanauki.pl/articles/2087926.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Polish Journal of Veterinary Sciences; 2017, 3; 429-437
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Outbreak of ranavirus infection in sheatfish, Silurus glanis (L.), in Poland
Autorzy:
Borzym, E.
Karpinska, T.A.
Reichert, M.
Powiązania:
https://bibliotekanauki.pl/articles/31663.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Ranavirus was detected in adult sheatfish, with clinical signs, on a Polish fish farm in February. Farm isolates induced a strong cytopathic effect in vitro and were identified by electron microscopy and PCR amplification of the ranavirus specific gene fragment. Restriction analysis with the Acc I enzyme showed that isolated ranaviruses were different from the epizootic heamatopoietic necrosis virus (EHNV). We sequenced a fragment of the major capsid protein (MCP) gene and found that isolates were similar to other strains of ranaviruses, available in GenBank.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiological situation of turkey coryza (bordetellosis) in Poland
Autorzy:
Smialek, M.
Tykalowski, B.
Pestka, D.
Stenzel, T.
Koncicki, A.
Powiązania:
https://bibliotekanauki.pl/articles/32424.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The present study investigated the prevalence of Bordetella avium (BA) infections in turkey flocks of different production type in Poland, based on serological examination of blood samples collected between 2012 and 2014. The results of our study indicate that BA infections are very common in turkey flocks, they are possible during the whole production cycle and that probability of the past infection increases with age. This situation is causing high level of specific anti-BA maternally derived antibody transfer to hatching poults.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 121

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