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Wyświetlanie 1-15 z 61
Tytuł:
Diurnal and seasonal changes in endogenous melatonin levels in the blood plasma in dogs
Autorzy:
Zan, R S
Rolinski, Z
Kowalski, C J
Bojarska-Junak, A
Madany, J
Powiązania:
https://bibliotekanauki.pl/articles/30357.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
This paper describes research on the levels of endogenous melatonin (MLT) in the blood serum in dogs in different seasons (March, June, September, December) and at different times of day (11:00, 12:00, 23:00, 24:00 and 1:00), using immunoassay method. Blood samples were collected in the diurnal cycle, in consecutive seasons. The conducted studies show that MLT levels undergo clear changes in both the diurnal cycle, as well as in seasonal one in this species.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hypertonic saline solution (NaCl 7.2%) enhances renal excretion of acids in cattle with acute ruminal lactic acidosis
Autorzy:
Rodrigues, F.A.M.L.
Minervino, A.H.H.
Barrêto Júnior, R.A.
Reis, L.F.
Ferreira, R.N.F.
Mori, C.S.
Oliveira, F.L.C.
Sousa, R.S.
Powiązania:
https://bibliotekanauki.pl/articles/2087583.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
steers
ruminal lactic acidosis
hypertonic saline solution
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 37-42
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A novel orchiectomy surgical procedure in donkeys (Equus asinus africanus) with parascrotal access
Autorzy:
Barrêto JR, R.A.
Rodrigues, L.A.
Albuquerque, J.P.
de Sousa, F.J.A.
Firmino, P.R.
Sousa, R.S.
Pedrosa, V.J.
do Amaral, T.R.
Minervino, A.H.H.
Powiązania:
https://bibliotekanauki.pl/articles/16539181.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
inflammation
donkeys
peritoneal fluid
surgical approach
castration
Opis:
Donkeys are a public health concern in the Northeast region of Brazil, with thousands of stray animals. Orchiectomy is an important population control measure; however, the long postoperative period with daily treatment of open wounds in the scrotum makes it difficult to perform a large number of castrations in sheltering centers. We evaluate a novel surgical procedure for orchiectomy in donkeys using parascrotal access. Twelve donkeys were used, divided into two groups: I - submitted to orchiectomy through parascrotal surgical access (novel procedure), and II - submitted to orchiectomy through scrotal access (conventional). Postoperative evaluations consisted of a macroscopic evaluation of the surgical wound (bleeding and intensity of edema), hematological parameters, and peritoneal fluid, which occurred in both groups at the moments (M): M0 - before the surgical procedure. The others moments occurred after surgery: M12 (twelve hours); M24 (twenty-four hours); M48 (forty-eight hours); M72 (seventy-two hours); M8D (eight days); and M16D (sixteen days). The surgical techniques did not generate an important systemic inflammatory response to the point detected by the leukogram, fibrinogen dosage, and peritoneal fluid. The parascrotal technique required long surgery but promoted less bleeding, less edema, and faster healing. The techniques used did not promote sufficient systemic inflammation to alter the number of leukocytes and the fibrinogen concentration; however, evaluation of the peritoneal fluid proved to be important for evaluating inflammatory processes involving the scrotum and inguinal canal. We describe a novel surgical procedure for orchiectomy in Donkeys using a parascrotal access that promoted less risk of bleeding, shorter period of edema, and healing time, but required longer surgery time.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 295-302
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Production and characterization of egg yolk antibody (IgY) against recombinant VP8 - S2 antigen
Autorzy:
Nasiri, K.
Nassiri, M.R.
Tahmoorespur, M.
Haghparast, A.
Zibaee, S.
Powiązania:
https://bibliotekanauki.pl/articles/30350.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. VP8 subunit of rotavirus is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response. Studies showed that immunoglobulin of egg yolk (IgY) from immunized hens has been identified to be a convenient source for specific antibodies for using in immunotherapy and immunodiagnostic to limit the infections. In this study, chimeric VP8-S2 gene was designed using by computational techniques. The chimeric VP8-S2 gene was cloned and sub-cloned into pGH and pET32a (+) vectors. Then, recombinant pET32a-VP8-S2 vector was transferred into E. coli BL21 CodonPlus (DE3). The expressed protein was purified by Ni-NTA chromatography column. Hens were immunized with the purified VP8-S2 protein three times. IgY was purified from egg yolks using polyethylene glycol precipitation method. Activity and specificity of anti-VP8-S2 IgY were detected by dot-blotting, Western-blotting and indirect ELISA. We obtained anti-VP8-S2 IgY by immunizing hens with the recombinant VP8-S2 protein. The anti-VP8-S2 IgY was showed to bind specifically to the chimeric VP8-S2 protein by dot-blotting, Western-blotting analyses and indirect ELISA. The result of this study indicated that such construction can be useful to investigate as candidates for development of detection methods for simultaneous diagnosis of both infections. Specific IgY against the recombinant VP8-S2 could be recommended as a candidate for passive immunization against bovine rotavirus and bovine coronavirus.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of acute lipopolysaccharide-induced toxemia model on some neglected blood parameters
Autorzy:
Adam, G.O.
Lee, H.R.
Lee, S.J.
Kim, S.J.
Kim, G.B.
Kang, H.S.
Powiązania:
https://bibliotekanauki.pl/articles/2087627.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Toxemia
lipopolysaccharide
base excess
pH
animal model
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 4; 665-671
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oxidative stress in cows according to calving season: passive calf immunity and its relationship with colostrum quality
Autorzy:
Çolakoğlu, H.E.
Yazlık, M.O.
Çolakoğlu, E.Ç.
Kaya, U.
Bayramoğlu, R.
Kurt, S.
Vural, R.
Küplülü, Ş.
Powiązania:
https://bibliotekanauki.pl/articles/2087184.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
calf
colostrum
cow
immunoglobulin
calving season
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 225-233
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Is downer cow syndrome related to chronic botulism?
Autorzy:
Rulff, R.
Schrodl, W.
Basiouni, S.
Neuhaus, J.
Kruger, M.
Powiązania:
https://bibliotekanauki.pl/articles/30507.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The present work was directed to investigate the relationship between Downer cow syndrome (DCS) and chronic botulism in dairy cattle. For this purpose, a total of 52 fresh calving downer cows and 206 apparently healthy cows at 14 dairy farms were investigated for Clostridium botulinum ABE and CD antibody levels, C. botulinum and botulinum neurotoxin in rumen fluids as well as in faeces. Results indicated that the downer cows had higher IgG titers for C. botulinum ABE and CD than the healthy cows. All tested rumen fluids were negative for BoNT and C. botulinum. BoNT/D, however, and C. botulinum type D spores were detected in faecal samples of healthy and downer cows in the selected farms. In conclusion, the presence of a significantly higher C. botulinum ABE and CD antibody levels in DCS cows than in the healthy animals suggests that chronic C. botulinum toxico-infection could be a predisposing factor for DCS.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
TaqMan real-time PCR for detecting bovine viral diarrhea virus
Autorzy:
Liang, H.
Geng, J.
Bai, S.
Aimuguri, A.
Gong, Z.
Feng, R.
Shen, X.
Wei, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087557.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bovine viral diarrhea virus
quantitative real time PCR
TaqMan probe
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 405-413
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical detection of P-glycoprotein in various subtypes of canine lymphomas
Autorzy:
Sokolowska, J.
Urbanska, K.
Gizinski, S.
Zabielska, K.
Lechowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/2087980.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Combination chemiotherapy is the current standard of care for dogs with lymphoma. Multidrug resistance is one of the most important factors contributing to the efficacy of chemiotherapy. The major protein responsible for this phenomenon is P-glycoprotein. Little is known about P-glycoprotein expression in particular subtypes of lymphomas. The aim of the study was evaluation of P-glycoprotein expression in various subtypes of canine lymphomas. Positive reaction with P-glycoprotein was found in 12/25 cases of various morphological subtypes of lymphomas, however, in 3/11 lymphomas the percentage of positively weakly stained cells was <10% and those tumors were also considered negative. Tumors with 10-50% P-glycoprotein positive cells were found in single cases of centroblastic and centroblastic-centrocytic tumors. In 5 lymphomas P-glycoprotein expression exceeded 50% of tumor cells. Those cases were found among centroblastic, centroblastic-centrocytic, lymphoblastic and Burkitt-like subtypes. Positive reaction was observed mainly in the cell cytoplasm, however, in some cases prominent perinuclear dot-like staining pattern was found. In 2 cases focal staining pattern comprised dominant type of immunolabelling. Among all lymphomas containing P-glycoprotein positive cells intensity of imunolabelling was assessed as weak (6/25), moderate (2/25) and strong (3/25). Our results indicate that P-glycoprotein expression is present in nearly one third of newly diagnosed canine lymphomas of different morphological subtypes including those most commonly occurring, such as cenroblastic lymphomas. Hence, determination of P-glycoprotein expression at the time of diagnosis could provide valuable information for the design of treatment protocols. Moreover, our results have shown that P-glycoprotein expression in canine tumors could be located in Golgi-zone.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular screening of gastric Helicobacter pullorum recovered from different avian species in Egypt
Autorzy:
Elhelw, R.
Elhariri, M.
Salem, H.M.
Khalefa, H.S.
Hamza, D.A.
Ahmed, Z.S.
Powiązania:
https://bibliotekanauki.pl/articles/16539461.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Helicobacter pullorum
PCR
sequencing
chicken
resident wild birds
zoonoses
Egypt
Opis:
Helicobacter pullorum ( H. pullorum) is a bacterium that colonizes the intestines of poultry and causes gastroenteritis. Because these species are known as human and/or animal pathogens, identification of H. pullorum is becoming increasingly necessary. The bacterium has been linked to colitis and hepatitis in humans after being transmitted by infected meat consumption. Misdiagnosis of other enteric zoonotic pathogens such as Campylobacter and other Helicobacter species makes the diagnosis of H. pullorum extremely difficult. This study focused on the molecular detection of H. pullorum from the stomach (proventriculus and gizzard) of different avian species as new target organs for detection and transmission between avian species. Proventriculus and gizzards were obtained from 40 freshly dead chickens and resident wild birds (n=40). Diarrhea was found in the farms that were surveyed. DNA was extracted from all collected samples to conduct PCR amplification. The samples were screened for Helicobacter genus-specific 16s using C97 and C05 primers. To confirm the existence of H. pullorum, the positive samples were sequenced. H. pullorum was recorded in two out of 40 chicken samples. In addition, H. pullorum was recorded in one out of 40 resident wild birds. The 16S rRNA gene sequence for Helicobacter genus-specific in poultry and wild birds showed a 100% homology. In conclusion, broiler chickens and resident wild birds are possible reservoirs for H. pullorum, according to this report, and possibly act as a source of infection for humans via the food supply.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 369-374
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunocytochemical detection of calretinin in the claustrum and endopiriform nucleus of the chinchilla
Autorzy:
Szalak, R.
Matysek, M.
Mozel, S.
Arciszewsk, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/30835.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Calretinin (CR) as a buffer and sensor protein plays an important role in regulatory processes of Ca²⁺ and anty-apoptotic cellular protection. In the present study, immunohistochemical peroxidase- antiperoxidase (PAP) method was used in order to determine the numbers, morphology, morphometry and distribution pattern of CR in neurons of the chinchilla’s claustrum (Cl) and endopiriform nucleus (EN). In Cl and EN the presence of several classes of neurons with different immunoreactivity to CR was found. In Cl, CR-immunoreactive (IR) neurons were predominantly found in close vicinity to insular border while CR-IR neurons were evenly scattered throughout EN. In general, immunoreaction to CR was observed in neuronal cytoplasm, nucleus and in fibres-like nerve extensions. Statistical analysis showed the differences between average large diameter as well as cross-sectional area of CR-IR neurons present in Cl and EN. It is suggested, that CR acting as a calcium binding protein may play a role in neuronal network. Further co-localization studies are necessary to fully elucidate neurophysiology and neuropathology of the chinchilla’s Cl and EN neurons.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk factors for outbreaks caused by variant strain of Newcastle disease on environmentally controlled broiler chicken farms in Lahore, Pakistan
Autorzy:
Maqsood, R.
Khan, A.
Mushtaq, M.H.
Yaqub, T.
Aslam, M.A.
Rashid, H.B.
Gill, S.S.
Akram, R.
Rehman, A.
Chaudhry, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087145.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Newcastle disease
retrospective
environmentally controlled house
biosecurity
vaccine
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 497-503
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep
Autorzy:
Khodabakhshzadeh, R.
Mohammadabadi, M.R.
Esmailizadeh, A.K.
Moradi Shahrebabak, H.
Bordbar, F.
Ansari Namin, S.
Powiązania:
https://bibliotekanauki.pl/articles/30643.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Screening the fertile ewes from national herds to detect the major genes for prolificacy is an effective way to create the fertile flocks. Growth differentiation factor (GDF) 9 is a member of the transforming growth factor β superfamily that is essential for folliculogenesis and female fertility. The aim of this study was to detect single nucleotide polymorphisms (SNPs) in exon 2 of GDF9 gene in Kermani sheep breed using PCR-SSCP. Genomic DNA was extracted from whole blood of collected samples using salting-out method. Whole exon 2 of GDF9 gene was amplified (634 bp and 647 bp fragments) using designed specific primers. The single stranded conformation polymorphism (SSCP) patterns of PCR products were studied using electrophoresis on acrylamide gel and silver-nitrate staining method. Finally, 4 banding patterns for the first primer pair and 4 banding patterns for the second primer pair were obtained. Also, indices of population genetic per SNP were calculated using Gen Alex 6.41 software. The sequencing results showed the presence of 3 mutations (SNP) (443, 477 and 721 positions) in the studied population.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Survivin expression in canine lymphomas in relation with proliferative markers
Autorzy:
Sokolowska, J.
Urbanska, K.
Gizinski, S.
Wysocka, A.
Cywinska, A.
Lechowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/2087990.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Survivin is a member of apoptosis inhibiting proteins family. Apart from its antiapoptotic activity it plays a critical role in regulating the cell cycle and mitosis. It is overexpressed in most human malignancies. While the prognostic significance of survivin expression is widely investigated in human non-Hodgkin's lymphomas, little is known about its expression in canine lymphomas. The aim of the study was to evaluate the expression of survivin in canine lymphomas in relation to proliferation markers (mitotic index and percentage of Ki67-positive cells). Survivin was found in all examined lymphomas belonging to 6 different morphological subtypes with nuclear immunoreactivity. In most of lymphomas (18/25) survivin expression ranged 10%-25% of positive cells. Only single cases had lower (0-10% positive cells, 1/25) or higher (25-50% and >50% positive cells, 5/25 and 1/25, respectively) index of survivin. Neither mitotic index nor proliferative index correlated with survivin expression when the values quantified randomly in whole specimens were compared. However, when survivin expression were quantified in selected tumor areas of low and high proliferation activity the high correlations between survivin expression and proliferation index were found. The results indicated that survivin is commonly expressed in canine lymphomas. Nuclear labelling together with the relation of its expression and proliferative activity in highly proliferative areas of neoplastic tissue suggest a potential role of survivin in cell cycle activation in canine lymphoma cells. However, further studies of the relation between expression of survivin and other proteins involved in cell cycle regulation are needed. Moreover, the results suggest that survivin may pose the therapeutic target in canine lymphomas.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of supplementing organic diets with fish meal and premix on the performance of pigs and some meat and blood characteristics
Autorzy:
Grela, E.R.
Matras, J.
Pisarski, R.K.
Sobolewska, S.
Powiązania:
https://bibliotekanauki.pl/articles/30373.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to determine the effect of fish meal and mineral-vitamin premix, supplementing organic diets, on the performance of pigs and some meat and blood characteristics. The experiment was conducted on an organic pig fattening farm. The study involved 120 pigs with an approximate 25 kg body weight. Animals were divided into 3 groups, kept in pens, 10 animals each. Group I (control) animals were fed with plant feedstuffs of organic origin. Diets for group II and III were enriched with fish meal or fish meal and vitamin-mineral premix, respectively. The experiment was carried out till pigs reached a weight of 115 kg. Feed samples were subjected to laboratory analyses. Body weight (3 times) and feed intake were recorded. Blood samples were collected (2 times) to determine hematological and biochemical indices. Some parameters in meat samples were also determined. The fish meal addition improved (P ≤ 0.05) the average daily gains as well as feed conversion ratio during fattening period and mineral-vitamin premix significantly (P ≤ 0.05) fortified fish meal influence. Fish meal supplement improved (P ≤ 0.05) also some carcass characteristics. Supplementation of the diet with premix additionally decreased (P ≤ 0.05) backfat thickness and increased share of meat in carcass. Fish meal improved (P ≤ 0.05) some meat characteristics and elevated content of some polyunsaturated fatty acids. An increase in hemoglobin, red blood cell, white blood cell and cholesterol level in blood of animals from both experimental groups were also found. The results obtained proved the usefulness of fish meal and mineral-vitamin premix in the fatteners nutrition based on organic diets.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 61

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