- Tytuł:
- Cytotoxicity and genotoxicity of GO-Fe3O4 hybrid in cultured mammalian cells
- Autorzy:
- Jedrzejczak-Silicka, M.
- Powiązania:
- https://bibliotekanauki.pl/articles/779480.pdf
- Data publikacji:
- 2017
- Wydawca:
- Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
- Tematy:
-
GO-Fe3O4 hybrid
relative viability
DNA fragmentation
micronucleus assay
genotoxicity - Opis:
- The study was aimed at investigating the effect of the Fe3O4 hybrid deposited on graphene oxide (GO- Fe3O4) on the relative viability and DNA integrity. The properties of the GO-Fe3O4 hybrid were analyzed using a transmission electron microscopy (TEM), X-ray diffraction technique (XRD) and thermal gravimetric method (TGA), while the efficiency of graphene oxide covalent functionalization with iron oxide nanospheres was determined by Fourier transform infrared spectroscopy (FT-IR). L929 and MCF-7 cell lines were selected to analyze the biocompatibility of GO-Fe3O4 nanoparticles. The hybrid was tested using WST-1 and LDH leakage assays. DNA integrity was analyzed by agarose gel electrophoresis and micronucleus assay was performed to examine chromosomal damage in the exposed cell lines. The tested GO-Fe3O4 hybrid did not significantly reduce cell metabolism of L929 cells. GO-Fe3O4 hybrid particles only slightly affected the integrity of cell membranes. DNA integrity and micronucleus assays did not indicate genotoxicity of the hybrid.
- Źródło:
-
Polish Journal of Chemical Technology; 2017, 19, 1; 27-33
1509-8117
1899-4741 - Pojawia się w:
- Polish Journal of Chemical Technology
- Dostawca treści:
- Biblioteka Nauki
Artykuł