- Tytuł:
- Evaluation of polyclonal and monoclonal antibodies for detection and determination of Clavibacter michiganensis subsp. Sepedonicus.
- Autorzy:
-
Pánková, Iveta
Kokošková, Blanka - Powiązania:
- https://bibliotekanauki.pl/articles/2198781.pdf
- Data publikacji:
- 2002-12-22
- Wydawca:
- Instytut Hodowli i Aklimatyzacji Roślin
- Tematy:
-
bacterial ring rot of potato
DAS ELISA
sensitivity
specificity
cross reactions - Opis:
- Immunochemical diagnostics of Clavibacter michiganensis subsp. sepedonicus (Spieckerman and Kotthoff) Davis et al., the causal agent of bacterial ring rot of potato, is based on the use of high quality polyclonal and monoclonal antibodies. The specificity and sensitivity of the polyclonal Po-Cs 6 and monoclonal Mn-Cs 1 antibodies for detection and identification of C. m. subsp. sepedonicus in pure culture and extracts of potato tuber samples were compared by double antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA). The polyclonal antibody Po-Cs 6 cross-reacted with strains of several Clavibacter michiganensis subspecies, Pantoea agglomerans, and weakly with Pseudomonas fluorescens and Ralstonia solanacearum (race 3)in all serological tests. After cross-absorption with these bacteria, the threshold level of homologous antigen NCPPB 3467 was 10H7 cfu/ml in DAS ELISA. The monoclonal antibody Mn-Cs 1 did not cross react with other michiganensis subspecies or with other plant pathogenic bacteria tested. The monoclonal antibody Mn-Cs 1 cross-reacted very weakly with the saprophytic bacteria P. agglomerans and P. fluorescens. The threshold level of homologous antigen NCPPB 3467 was 104 cfu/ml in DAS ELISA. The monoclonal antibody Mn-Cs 1 proved to be more sensitive and specific than the polyclonal antibody Po-Cs 6 in all serological tests.
- Źródło:
-
Plant Breeding and Seed Science; 2002, 46, 2; 17-24
1429-3862
2083-599X - Pojawia się w:
- Plant Breeding and Seed Science
- Dostawca treści:
- Biblioteka Nauki
Artykuł