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Wyszukujesz frazę "electrophoresis" wg kryterium: Temat


Wyświetlanie 1-5 z 5
Tytuł:
Genetic diversity of winter wheat cultivars and strains determined by electrophoregrams of gliadin and glutenin proteins
Autorzy:
Węgrzyn, Stanisław
Waga, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/2198933.pdf
Data publikacji:
2004-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
cluster analysis
electrophoresis
gliadins
glutenins
polymorphism
Opis:
Based on the polymorphism of gliadin and glutenin proteins relationships of 45 cultivars and strains of winter wheat were evaluated. The cluster analysis showed a considerable variation of the investigated genotypes. The similarity indices were calculated using the Nei and Li formula. The genetic distances between the cultivars ranged from 1.00 to 0.12. The highest similarity index - SI=1.00- being proof of the identical physicochemical composition of storage proteins, was found for the pair Farmer and Elena. The groups of similar and genetically distant cultivars have been presented in the form of a dendrogram. The possibility of using the results obtained from the cluster analysis in breeding programmes has been discussed.
Źródło:
Plant Breeding and Seed Science; 2004, 49; 51-61
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic characterization of maize inbred lines and their mutual affinities.
Autorzy:
Bulińska-Radomska, Z.
Adamczyk, J.
Królikowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2198825.pdf
Data publikacji:
2001-06-21
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Inbred lines
enzymes
electrophoresis
similarity
pedigree
heterosis
maize
Opis:
Twenty-five inbred lines of maize assigned to six heterotic groups and developed in Poland were studied electrophoretically to determine their purity and mutual relationships. The electrophoretic data were analyzed by principal component and cluster analyses. All lines (except five) were monomorphic in each enzyme studied. Electrophoretic affinities of inbred lines were, with some exception, were in accordance with the expectation inferred from their pedigrees. Lines of identical pedigree but with small differences in phenotypic or developmental characteristics had the lowest dissimilarity coefficient values and in cluster and principal component analyses behaved accordingly. They showed however diverse levels of clustering and dissimilarity coefficient values, which most likely reflected their departure from the common parental gene pool, which occurred in the course of breeding manipulations. Lines, which had one parent in common, were less similar to each other than those with identical pedigree. The most distinct electrophretically were lines with complex or dissimilar pedigree formulae as was confirmed by cluster and principal component analyses.
Źródło:
Plant Breeding and Seed Science; 2001, 45, 1; 11-19
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphism of the storage proteins in rye cultivars and populations selected for tolerance to nutrient deficiency.
Autorzy:
Rzepka-Plevneš, Danuta
Smolik, Miłosz
Powiązania:
https://bibliotekanauki.pl/articles/2198912.pdf
Data publikacji:
2003-12-21
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
differences
electrophoresis
in vitro cultures
nutrient deficit
rye
storage protein
tolerance
Opis:
Ten open-pollinated cultivars, two strains of rye and Sl, S2, S3 progenies obtained after selection directed towards tolerance to nutrient deficit were used in the study. The aim of the study was to compare the genotypes of the above mentioned rye populations by electrophoretically characterised of their secalin patterns.The result showed that most of the tested rye cultivars revealed simi1ar but not identica1 electrophoretic patterns. Specific secalin patterns were obtained from all the cultivars, except cv. Arant and strain SMH 92. Differences in comparison to initial genotypes were observed in some rye populations in Sl and S2 generations, including additional polypeptide of molecular weight 43 kDa. It was not linked with rye tolerance to nitrogen and potassium deficit in medium.
Źródło:
Plant Breeding and Seed Science; 2003, 48; 49-59
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inheritance of some w-gliadin protein subunits in spelt wheat and their linkage with the red glume coding Rg-1 locus.
Autorzy:
Waga, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/2198843.pdf
Data publikacji:
2002-12-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
A PAGE electrophoresis
linkage analysis
protein blocks
gliadin subunits
glume colour
Opis:
The Gli B1 locus controlling the synthesis of certain fractions of wheat storage proteins belonging to the group of ω-gliadins is tightly linked with the Rg1 gene responsible for red glume colour. The inheritance of selected ω-gliadin subunits and of glume colour was studied in order to identify markers of this trait among protein fractions specific to spelt wheat. Electrophoretic analysis for 217 single plants of the F2 generation in the cross Tr. aestivum (cultivar Elena) × Tr. spelta was done. Four out of five protein subunits specific for spelt wheat (O1, O3, O4 and O5) as well as gliadin block Gli B1-1 of the Elena cultivar were inherited according to Mendelian segregation ratio 1:2:1 assumed for two allelic protein variants of one gene. The above mentioned fractions of spelt wheat formed a block, which has been denoted by the symbol Gli B1-6. The results of segregation analysis regarding glume colour indicate Mendelian, single-gene inheritance of this trait (3:1 ratio of red and white glumes), which differs from the results of other investigations suggesting the existence of two genes controlling the red colour. The genetic distance between loci Gli B1 and Rg1 has been estimated to be 1.9 cM.
Źródło:
Plant Breeding and Seed Science; 2002, 46, 2; 25-35
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of glutenin and secalin subunits in addition and substitution lines of wheat (Triticum aestivum L.) using biochemical markers
Autorzy:
Chrząstek, Maria
Powiązania:
https://bibliotekanauki.pl/articles/2199010.pdf
Data publikacji:
2005-12-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
addition lines
HMW glutenins
LMW glutenins
1B/1R substitution line
SDS-PAGE electrophoresis
secalins
Opis:
The aim of paper was evaluation of composition of high molecular weight (HMW) and low molecular weight (LMW) glutenin subunits in wheat-rye addition lines cv. Grana - cv. Dańkowskie Złote, substitution line 1B/1R and initial forms, as well as, localization of Sec genes on chromosomes ‘Dańkowskie Złote’ rye. Analysis were performed using SDS-PAGE electrophoresis.The composition of HMW glutenin for all addition lines and ‘Grana’ wheat was: N - 6+8 - 2+12. Composition of LMW glutenin was estimated only for 2R and 6RL addition lines, wheat, and octoploid triticale. In both addition lines the subunits N-6+9-15 were identified, in wheat cv. Grana subunits 1-6+9-15 were present, whereas in octoploid triticale polymorphism was observed (1-6+9-15 or 1-11+13-N). Rye storage proteins (secalins) was found in addition lines: 1R, 2R and substitution line 1B/1R. It can be concluded that genes responsible for secalin biosynthesis in rye cv. Dańkowskie Złote are localized on 1R and 2R chromosomes.
Źródło:
Plant Breeding and Seed Science; 2005, 52; 79-86
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-5 z 5

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