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    Wyświetlanie 1-11 z 11
    Tytuł:
    Zastosowanie markerow DNA [RAPD, SSR, PCR-RFLP i STS] w genetyce drzew lesnych, entomologii, fitopatologii i lowiectwie
    Molecular markers [RAPD, SSR, PCR-RFLP and STS] in forest-tree genetics, entomology, phytopathology and game management
    Autorzy:
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/45939.pdf
    Data publikacji:
    2006
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lowiectwo
    zastosowanie
    genetyka roslin
    markery genetyczne
    entomologia lesna
    metody badan
    genetyka zwierzat
    lesnictwo
    zwierzeta lowne
    metoda SSR
    metoda RAPD
    metoda PCR-RFLP
    metoda STS
    drzewa lesne
    fitopatologia lesna
    DNA
    Źródło:
    Leśne Prace Badawcze; 2006, 1; 73-101
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Charakterystyka zmiennosci genetycznej sosny napiwodzko-ramuckiej i spalskiej na podstawie analiz mitochondrialnego DNA
    Autorzy:
    Nowakowska, J.
    Rakowski, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/45663.pdf
    Data publikacji:
    2005
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    DNA mitochondrialny
    sosna napiwodzko-ramucka
    zmiennosc genetyczna
    markery genetyczne
    genetyka roslin
    populacje roslin
    sosna zwyczajna
    lesnictwo
    struktura genetyczna
    sosna spalska
    Pinus sylvestris
    drzewa lesne
    Źródło:
    Leśne Prace Badawcze; 2005, 2; 73-91
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zmienność genetyczna naturalnego odnowienia i drzewostanu macierzystego sosny (Pinus sylvestris L.) i świerka (Picea abies L. Karst.)
    Genetic variability of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies L. Karst.) natural regeneration compared with their maternal stands
    Autorzy:
    Nowakowska, J.A.
    Zachara, T.
    Konecka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1206918.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    drzewostany macierzyste
    odnowienia naturalne
    drzewa lesne
    sosna zwyczajna
    Pinus sylvestris
    swierk pospolity
    Picea abies
    zmiennosc genetyczna
    Opis:
    The genetic variability and biodiversity of tree populations ensure the stability and sustainability of forest ecosystems. New research tools based on molecular DNA markers enable precise characterisation of forest genetic resources, i.e. detection of different allele frequencies in mature trees and progeny populations. The paper describes the genetic structure of mature stands of Scots pine (Pinus sylvestris L.) in Oława Forest District and Norway spruce (Picea abies L. Karst.) in Jawor Forest District and stands of their respective progeny. In the Scots pine stand, there was a slight increase (0.6%) in heterozygosity level and a larger increase (4.9%) in the inbreeding coefficient of progeny trees. In the Norway spruce stand, despite a small reduction (0.9%) in heterozygosity, a similar increase (4.6%) in the inbreeding coefficient of progeny was revealed. In both stands, allele richness and the partition probability of basic clustering were high. Both pine and spruce adults and progeny trees were characterised by high levels of genetic similarity (96% and 79%, respectively). Gene flow between the mature and progeny populations was high (Nm > 1) for both Scots pine and Norway spruce. Conservation of the gene pool within forest tree stands requires an increase in the proportion of natural regeneration. To estimate the extent to which genes are transmitted between adult trees and their progeny, more studies are needed, especially taking into account the influence of silviculture measures, like selective tree cutting, on the genetic variability of the younger generation. These results confirm that the gene pool was conserved when transmitted between the stands studied, as well as highlight the usefulness of such a study for silvicultural purposes.
    Źródło:
    Leśne Prace Badawcze; 2014, 75, 1; 47-54
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Aktualne możliwości i perspektywy wykorzystania fungicydów w leśnictwie
    Current possibilities and prospects of using fungicides in forestry
    Autorzy:
    Okorski, A.
    Pszczółkowska, A.
    Oszako, T.
    Nowakowska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/1310990.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    szkolki lesne
    ochrona lasu
    ochrona roslin
    fungicydy
    ditiokarbaminiany
    amidy kwasu karboksylowego
    karbaminiany
    fosfoniany
    morfoliny
    triazole
    aminopirymidyny
    tlenochlorek miedziowy
    pochodne weglowodorow aromatycznych
    pirydynokarboksyamidy
    fungicydy strobilurynowe
    benzimidazole
    stosowanie
    forest protection
    forest nurseries
    fungicide resistance
    fungicides’ mode of action
    Opis:
    The possibility of using chemicals in European forestry is extremely limited due to the binding legal regulations and specific conditions concerning the market of plant protection products. This is reflected in the limited availability of active fungicides in forestry. Due to this limitation, practitioners using fungicides in forest nurseries and forest cultivation must have substantial knowledge of the biology of pathogens to ensure satisfactorily effective protection. The work presented here provides an overview of the currently recommended fungicides in Polish forestry as well as the mechanisms of interaction between the active substances and the pathogen, the plant and mycorrhizal fungi. In this paper we also discuss the risk of fungicide resistance, which has been insufficiently explored in the context of forest pathogens.
    Źródło:
    Leśne Prace Badawcze; 2015, 76, 2; 191-206
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zamieranie olszy czarnej Alnus glutinosa (L.) Gaertn. wzdłuż rzeki Narewki na terenie Nadleśnictwa Białowieża
    Decline of Black Alder Alnus glutinosa (L.) Gaertn. along the Narewka River in the Białowieża Forest District
    Autorzy:
    Malewski, T.
    Topor, R.
    Nowakowska, J.A.
    Oszako, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2142614.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    alder
    dieback
    drought
    Phytophthora
    pcr
    olsza
    zamieranie
    susza
    Opis:
    Black Alder Alnus glutinosa (L.) Gaertn. is an important tree commonly growing in Poland. Alders are actinorhizal plants that play an important ecological role in riparian ecosystems through atmospheric nitrogen fixation, filtration and purification of waterlogged soils as well as providing a refuge for terrestrial and aquatic organisms thus helping to stabilize stream banks. Black Alder used to be considered a very pest and disease resistant species but, the situation changed in 2000, when an unprecedented decline of Alders was observed in Poland. In the Białowieża Forest District, this decline has been observed on wet meadow habitats and along rivers or watercourses. Currently, there are several hypotheses explaining Alder dieback, among them climatic changes and Phytophthora infections. In terms of climate, Black Alder requires a high atmospheric humidity during all phases of its reproductive cycle. It tolerates neither long-term summer flooding nor a significant decrease in the groundwater level. In terms of pests, oomycete pathogens of the genus Phytophthora are the most destructive plant pathogens known and many of them are present in forests and nurseries all over Europe. The aim of this study was to evaluate the health of Black Alder along the Narewka River in the Białowieża Forest District. Selected areas were monitored in 2012 and 2018, but no relationship between drought and alder health was found. A preliminary analysis of soil and water samples by real time PCR revealed the presence of two Phytophthora species: P. alni and P. cactorum. Further and more detailed research is required to elucidate the role of these pathogens in Alder dieback.
    Źródło:
    Leśne Prace Badawcze; 2020, 81, 4; 147-152
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Charakterystyka genetyczna PCR-RFLP oraz ocena zdrowotnosci wybranych populacji debu elblaskiego i krotoszynskiego
    Genetic characterization in relation to the health state of oak populations in the Elblaski i Krotoszynski regions of Poland
    Autorzy:
    Nowakowska, J.A.
    Oszako, T.
    Bieniek, J.
    Rakowski, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/45956.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    dab elblaski
    dab krotoszynski
    zmiennosc genetyczna
    populacje roslin
    ekotypy
    Quercus robur
    lesnictwo
    zdrowotnosc drzew
    DNA chloroplastowy
    dab szypulkowy
    metoda PCR-RFLP
    drzewa lesne
    Źródło:
    Leśne Prace Badawcze; 2007, 3; 33-51
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Możliwości biologicznej ochrony roślin przed chorobami w szkółkarstwie, ze szczególnym uwzględnieniem lęgniowców (Oomycetes) i grzybów z rodzaju Fusarium
    The possibilities of biologically protecting plants against diseases in nurseries, with special consideration of Oomycetes and Fusarium fungi
    Autorzy:
    Okorski, A.
    Oszako, T.
    Nowakowska, J.A.
    Pszczółkowska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1312848.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    szkolkarstwo lesne
    ochrona lasu
    ochrona roslin
    szkolki lesne
    czynniki chorobotworcze
    legniowce
    Oomycetes
    Phytophthora
    Phytium
    Fusarium
    biologiczne metody ochrony roslin
    organizmy antagonistyczne
    zwalczanie chorob roslin
    forest protection
    forest nurseries
    biological control
    Opis:
    Achieving high quality propagative material is difficult today due to the limited number of pesticides recommended for use. Simultaneously, EU regulations on Integrated Pest Management (IPM) in forest nurseries came into a force, requiring a search for alternative plant protection methods that are safe for humans, animals and the environment. In this paper, we present the possibilities of using bio-fungicides against diseases in forest nurseries, their mechanisms of action, as well as the direction of their development (according to IPM rules). We reviewed the results achieved by different research teams presenting the possibilities and trends in combatting Oomycetes and Fusarium spp. pathogens currently having the most important economic impact.
    Źródło:
    Leśne Prace Badawcze; 2014, 75, 3; 301-321
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Filtry piaskowe jako element zintegrowanej ochrony materiału rozmnożeniowego w szkółkach leśnych
    Slow Sand Filters as a part of integrated protection of seedlings against disease in forest nurseries
    Autorzy:
    Oszako, T.
    Kubiak, K.A.
    Siebyła, M.
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1318288.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    szkolki lesne
    ochrona roslin
    woda uzytkowa
    oczyszczanie wody
    filtry piaskowe
    biofiltry
    skutecznosc
    redukcja mikroorganizmow
    Phytophathogen
    biofilm
    SSF
    PCNB
    mineral fertilizer
    Opis:
    Slow Sand Filters (SSF) are a biological method used to protect nursery plants, from pathogen infections which can cause serious diseases in many forest tree species. Thanks to SSF application the number of phytopathogens in nurseries can be significantly reduced, as demonstrated by many field and greenhouse experiments (e.g. in Polish nurseries, and for horticultural crops in Germany and The Netherlands). In this study, the effect of pollution from fertilizers and fungicides used in agriculture (e.g. PCNB) on the efficiency of SSFs was assessed. A quantitative analysis was performed of the copiotrophic and oligotrophic bacterial composition colonizing SSF biofilms. The efficiency with which selected Oomycete strains belonging to the genus Phytophthora (P. alni, P. cactorum, P. plurivora) were removed from water was determined based on genetic material (DNA of the organisms) found in the SSF filtrate. Specific primers and TaqMan probes (qPCR) appeared to be the most sensitive molecular methods. Moreover, the microbiological analysis of SSF biofilm performed with selective media allowed the growth of copiothrophic and oligothrophic bacteria to be estimated. The influence of fungicide (PCNB) and N-fertilizer on the number of bacteria in each biofilm was also evaluated. The pollution of water with fertilizer (being used for plant irrigation) was demonstrated to reduce the efficiency of filtration more than fungicide addition (the amount of DNA from those investigated pathogens in the water decreased with time). The amount of bacteria in SSF biofilm readily increased after application of N-fertilizer in contrast to fungicide (PCNB) addition.
    Źródło:
    Leśne Prace Badawcze; 2013, 74, 1; 49-56
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Multipleksowa detekcja gatunków Phytophthora przy użyciu platformy Fluidigm
    Multiplex detection of Phytophthora spp. using the Fluidigm platform
    Autorzy:
    Sikora, K.
    Oszako, T.
    Kubiak, K.
    Nowakowska, J.A.
    Malewski, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2142612.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    forest soil
    oomycetes
    oak dieback
    pathogens
    next generation sequencing
    gleby leśne
    oomycety
    zamieranie dębów
    patogeny
    sekwencjonowanie nowej generacji
    Opis:
    The genus Phytophthora plays an important role not only in agriculture but also in forest ecosystems. As the number of known Phytophthora species continues to grow, identifying new isolates in this genus has become increasingly challenging even by DNA sequencing. Therefore, the development of proper techniques for detection and identification is crucial for monitoring and control of these pathogens in the forestry sector. In recent years, new molecular methods using innovative approaches have indeed been developed. However, the majority of these methods was designed to detect single Phytophthora species. Techniques that are able to target multiple species would offer advantages, especially for the assessment of Phytophthora diversity in the environment. This paper describes a multiplex assay for the identification of eight Phytophthora isolates, down to the species level, based on a Fluidigm platform employing pyrosequencing. The obtained results showed that for an accurate determination of the species, it is sufficient to know the sequence of two markers, ITS and COX1. The sensitivity of this test is sufficient to identify Phytophthora in a pure culture. Unfortunately, analysis based on a pyrosequencing platform does not provide enough data to simultaneous identify multiple Phytophthora species in samples collected in the field. This problem could be resolved in the future by sequencing using more efficient platforms like Illumina or IonTorrent.
    Źródło:
    Leśne Prace Badawcze; 2020, 81, 4; 161-166
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wykorzystanie związków fosforynowych w leśnictwie
    The use of phosphates in forestry
    Autorzy:
    Tkaczyk, M.
    Kubiak, K.A.
    Sawicki, J.
    Nowakowska, J.A.
    Oszako, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/45658.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    fosforyny
    nawozy
    fungicydy
    biostymulatory
    zastosowanie
    lesnictwo
    Źródło:
    Leśne Prace Badawcze; 2016, 77, 1
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stan zdrowotny a zróżnicowanie genetyczne wybranych drzewostanów świerkowych na terenie RDLP w Krośnie
    Genetic variability and health of Norway spruce stands in the Regional Directorate of the State Forests in Krosno
    Autorzy:
    Gutkowska, J.
    Borys, M.
    Tereba, A.
    Tkaczyk, M.
    Oszako, T.
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1293265.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    RDLP Krosno
    drzewostany swierkowe
    stan zdrowotny lasu
    drzewa lesne
    swierk pospolity
    Picea abies
    zroznicowanie genetyczne
    markery molekularne
    markery mikrosatelitarne
    DNA mitochondrialny
    Norway spruce
    health state
    SSR markers
    mtDNA
    genetic diversity
    Opis:
    The study was conducted in 2015 in six spruce stands situated in different forest districts administratively belonging to the Regional Directorate of Forests State in Krosno. Each spruce population was represented by 30 trees and assessed in terms of their current health status. Genetic analyses were performed based on shoot samples from each tree using nine nuclear DNA markers and one mitochondrial DNA marker (nad1). The health status of the trees was described according to the classification developed by Szczepkowski and Tarasiuk (2005) and the correlation between health classes and the level of genetic variability was computed with STATISTICA (α = 0.05). Nuclear DNA analyses revealed a low level of genetic variability among spruce populations (only 3% of the total genetic variation (FST = 0.028) and a high variability within populations (97%). The total heterozygosity in all stands (HT) was calculated as 0.646. Based on UPGMA analysis, the most genetically similar populations are spruce stands in the Bieszczady National Park and the Ustrzyki Dolne Forest District, which have the smallest genetic divergence of all populations (DN = 0.0165). Our analysis of the mitochondrial gene nad1 revealed the presence of six different haplotypes “a”, “a1”, “b”, “c”, “d” and “d1”. Comprising 56% of all haplotypes, “a” was the most common showing a predominant impact on spruce migration from the Carpathian area. The analysis based on mitochondrial markers (by Nei) revealed a heterozygosity of 0.525. Based on the observations of disease symptoms, 29% of the trees belong to health class 1,30% to class 2,28% to class 3 and class 4 contains 13% of trees. The comparison between health status and the level of genetic variation in the analyzed stands showed a positive correlation. Spruce stands with better health were also characterized by a greater degree of genetic variability. Since most of the investigated spruce populations shared the mitochondrial haplotype “a”, we have ascertained their Hercynian- Carpathian origin. Only one stand (Cisna) had a high frequency (43.3%) of the Nordic haplotype “c” suggesting that this provenance is derived from the Baltic post-glacial refugium of P. abies in Europe.
    Źródło:
    Leśne Prace Badawcze; 2017, 78, 1
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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