Autor: Mach, J. - Katalog OPAC zbiorów

Tytuł:: Increased neurogenesis after ACEA and levetiracetam treatment in mouse pilocarpine model of epilepsy
Autorzy:: Zagaja, Mirosław
Szewczyk, Aleksandra
Haratym-Maj, Agnieszka
Rola, Radosław
Maj, Maciej
Łuszczki, Jarogniew J.
Anders-Mach, Marta
Powiązania:: https://bibliotekanauki.pl/articles/972512.pdf
Data publikacji:: 2017
Wydawca:: Instytut Medycyny Wsi
Tematy:: neurogenesis
neurons
astrocytes
pilocarpine
ACEA
Levetiracetam
Opis:: Introduction and objectives. The aim of the study was to asses the impact of long-term therapy with the second generation antiepileptic drug levetiracetam (LEV) with arachidonyl-2’-chloroethylamide (ACEA), a highly selective cannabinoid CB1 receptor agoniston the process of neurogenesis in a mouse pilocarpine model of epilepsy (PILO). Additionally, a relationship was established between the treatment with ACEA in combination with LEV, and hippocampal neurogenesis in mouse PILO brain. Materials and method. All experiments were performed on adolescent male CB57/BL mice injected i.p. with LEV (10 mg/kg), ACEA (10 mg/kg) and PMSF (30 mg/kg) (phenylmethylsulfonyl fluoride — a substance protecting ACEA against degradation by the fatty-acid amidohydrolase), pilocarpine (PILO, a single dose 290 mg/kg) and methylscopolamine (30 min before PILO to stop the peripheral cholinergic effects of the pilocarpine, 1 mg/kg). The process of neurogenesis was evaluated after10 days treatment with LEV and ACEA. Results. Obtained results indicated that the combinations of ACEA+PMSF+LEV and ACEA +PMSF increased the total number of total newborn cells compared to the control. Moreover, ACEA+PMSF administered alone and in combination with LEV had a significant impact on neurogenesis increasing the total number of newborn neurons compared to the control group. Neither LEV nor PMSF had a significant impact on the number of proliferating cells and newborn neurons when compared to the control PILO group. In turn, LEV administered alone decreased significantly the number of astrocytes. However, ACEA+PMSF has demonstrated significant increase of astrocytes compare to control mice. Conclusions. These data provide substantial evidence that the combination of LEV+ACEA significantly increases the level of newborn neurons in the PILO dentate subgranular zone.
Źródło:: Journal of Pre-Clinical and Clinical Research; 2017, 11, 2; 136-141
1898-2395
Pojawia się w:: Journal of Pre-Clinical and Clinical Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Interactions of retigabine with topiramate in the mouse tonic-clonic seizure model and chimney test - an isobolographic analysis
Autorzy:: Zagaja, Mirosław
Miziak, Barbara
Kondrat-Wróbel, Maria W.
Andres-Mach, Marta
Wróblewska-Łuczka, Paula
Adamczuk, Piotr
Chmura, Robert
Czuczwar, Stanisław Jerzy
Łuszczki, Jarogniew J.
Powiązania:: https://bibliotekanauki.pl/articles/972722.pdf
Data publikacji:: 2017
Wydawca:: Instytut Medycyny Wsi
Tematy:: maximal electro-shock
drug interaction
isobolographic analysis
Retigabine
Topiramate
Opis:: Introduction and objectives. Nowadays, one of the treatment options for patients with refractory epilepsy is polytherapy with two or more antiepileptic drugs (AEDs). Retigabine (RTG) is a novel third-generation AED with unique molecular mechanisms of action that has recently been approved as an add-on drug for the treatment of tonic-clonic seizures. To characterize types of interactions between RTG and topiramate (TPM – a second-generation AED), the maximal electroshock- induced seizure model (MES) and chimney test in mice were used. Materials and method. In the MES model, the anticonvulsant effects of the drugs in terms of suppression of tonic-clonic seizures in male albino Swiss mice were assessed. In the chimney test, the acute neurotoxic effects of the drugs with respect to impairment of motor coordination were determined. Type I isobolographic analysis for the combination of RTG and TPM was applied to assess the anticonvulsant and neurotoxic effects in both the MES and chimney tests. Total brain concentrations of RTG and TPM were measured to exclude any pharmacokinetic interaction between drugs. Results. The type I isobolographic analysis of interaction revealed that the combination of RTG with TPM produced additive interaction in the MES test and additivity, with a slight tendency towards antagonism in terms of acute neurotoxic effects in the chimney test. Neither RTG nor TPM mutually affected total brain concentrations in the experimental animals. Conclusions. The isobolographically analyzed combination of RTG with TPM is favourable and may be recommended to some patients with refractory epilepsy.
Źródło:: Journal of Pre-Clinical and Clinical Research; 2017, 11, 1; 61-65
1898-2395
Pojawia się w:: Journal of Pre-Clinical and Clinical Research
Dostawca treści:: Biblioteka Nauki

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