Temat: phylogenetic analysis - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Phylogenetic analysis and genetic structure of new isolates of Tomato mosaic virus in Iran
Autorzy:: Rakhshandehroo, F.
Hashemi, S.S.
Shahraeen, N.
Powiązania:: https://bibliotekanauki.pl/articles/961661.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: phylogenetic analysis
genetic structure
new isolate
tomato mosaic virus
genetic differentiation
genetic variation
phylogenetic tree
Tobamovirus
Iran
Opis:: The present report describes the new occurrence of Tomato mosaic virus (ToMV) in cabbage, bean and Malva neglecta plants in Iran. In this study, sequence analyses of a partial RNA dependent RNA polymerases (RdRp) and complete movement protein (MP) and the coat protein (CP) nucleotide sequences of three new ToMV isolates collected from major crop fields in Iran revealed low genetic variation of RdRp gene compared to the CP and MP genes. The different topologies of the phylogenetic trees constructed, using available open reading frame (ORF1), ORF2 and ORF3 sequences from ToMV isolates, indicated different evolutionary constraints in these genomic regions. Statistical analysis also revealed that with the exception of CP other tested ToMV genes were under negative selection and the RdRp gene was under the strongest constraints. According to the phylogenetic tree it can be inferred from the nucleotide sequences of the complete CP and MP genes, that isolates from Iran and Egypt formed separate groups, irrespective of host origin. However, isolates clustered into groups with correlation to geographic origin but not the host. Analysis of the Ks*, Z* and Snn values also indicated genetic differentiation between ToMV populations. The Tajima’s D, Fu and Li’s statistical values were significantly negative for the RdRp gene of the Asian population which suggests the sudden expansion of ToMV in Asia. Taken together, the results indicate that negative selection and genetic drift were important evolutionary factors driving the genetic diversification of ToMV.
Źródło:: Journal of Plant Protection Research; 2018, 58, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Prevalence and phylogenetic analysis of Fig mosaic virus and Fig badnavirus-1 in Iran
Autorzy:: Alimoradian, M.
Rakhshandehroo, F.
Shams-bakhsh, M.
Powiązania:: https://bibliotekanauki.pl/articles/66369.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: prevalence
phylogenetic analysis
Fig mosaic virus
Fig badnavirus-1
Iran
fig tree
plant disease
Opis:: Fig mosaic virus (FMV) and Fig badnavirus-1 (FBV-1) are two of the most important fig infecting viruses. The incidence and distribution of FBV-1 and FMV were determined by testing in PCR 138 asymptomatic and symptomatic samples. These samples were collected from 60 fig gardens and agricultural fields in three provinces of Iran. The fig infecting viruses FBV-1 and FMV, respectively, were detected in 92 (66.6%) and 34 (24.6%) samples collected from all the surveyed fields. Overall, 24 out of 138 (17.3%) samples showed mixed infections. The sequence analysis of a genomic fragment of 922 nt, comprising the entire ORF-2 and part of the 5’ termini of the ORF-3 of 10 selected FBV-1 Iranian isolates from different provinces, and of the type member from GenBank (Acc. No: JF411989), showed a variation ranging from 1 to 3% at nucleotide level and 1% at the amino acid level. The phylogenetic analysis grouped the FBV-1 isolates into two groups, with the Iranian isolates clustered in two distinct subgroups of group I, according to their geographical origin. In our research, the prevalence and sequence analysis of FBV-1 as the only identified DNA virus infecting fig trees, was studied for the first time in Iran.
Źródło:: Journal of Plant Protection Research; 2016, 56, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Molecular identification of pathogenic Fusarium species, the causal agents of tomato wilt in western Iran
Autorzy:: Chehri, K.
Powiązania:: https://bibliotekanauki.pl/articles/66185.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: molecular identification
pathogen
Fusarium
causal agent
tomato
Lycopersicon esculentum
wilt pathogen
pathogenicity
phylogenetic analysis
Iran
Opis:: Fusarium species are causal agents of fungal diseases occurring frequently in numerous agriculturally important plants, including potato, garlic and are one of the common pathogens of tomato, causing root rot in the west part of Iran. Therefore, the objectives of this study were to isolate and identify disease-causing Fusarium species from infected tomatoes based on the morphological and molecular characteristics. Twenty-five isolates of Fusarium were obtained from infected root of tomato plants collected from the fields in different regions of western Iran. Based on morphological features, the strains were classified into four following Fusarium species: F. oxysporum, F. redolens, F. proliferatum and F. verticillioides. The phylogenetic trees based on tef1 and tub2 dataset clearly distinguished closely related species. All of the isolates were evaluated for their pathogenicity on healthy tomato seedlings in the greenhouse. This is the first report on molecular identification of Fusarium species isolated from tomato plants cultivated in Iran.
Źródło:: Journal of Plant Protection Research; 2016, 56, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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