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Wyświetlanie 1-4 z 4
Tytuł:
Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew
Autorzy:
Niranjan-Raj, S.
Lavanya, S.N.
Nayaka, S.C.
Powiązania:
https://bibliotekanauki.pl/articles/2084674.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
downy mildew
plant defense
Pennisetum glaucum
polyphenol oxidase
Opis:
Polyphenol oxidase partial gene PG-PPO was cloned and characterized from Pennisetum glaucum (pearl millet) which showed 42% identity to a PPO sequence isolated from wheat at the region of Copper B with a score of 40 and e-value of 2.8. Multiple sequence alignment results revealed similarity to polyphenol oxidase (PPO) sequences from wheat, trifolium, lettuce, apricot, tobacco, tomato, pokeweed, apple, grape and poplar especially at the Copper B region of PPO. The 395 bp pearl millet PPO sequence was AT rich (53.3%) and contained the highly conserved amino acids of histidine-rich copper binding sites similar to PPO sequences from other crops. Results also indicated that PPO in pearl millet exists in multi copy. The role of the isolated PPO gene during pearl millet-downy mildew interaction was analyzed and the results showed significantly higher and rapid accumulation of PPO mRNAs in resistant pearl millet seedlings inoculated with Sclerospora graminicola in comparison to the susceptible control, demonstrating that the PPO plays a prominent role in pearl millet defense against pathogens, particularly downy mildew pathogen.
Źródło:
Journal of Plant Protection Research; 2019, 59, 3; 423-427
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical constituents and ovicidal effects of mahlab, Prunus mahaleb L. kernels oil on cotton leafworm, Spodoptera littoralis (Boisd.) eggs
Autorzy:
Mead, H.M.
El-Shafiey, S.N.
Sabry, H.M.
Powiązania:
https://bibliotekanauki.pl/articles/65600.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cotton leafworm
chemical constituent
enzyme
mahlab zob.mahleb
mahleb
spice
Prunus mahaleb
oil
Spodoptera littoralis
egg
Opis:
The carried out investigations evaluated ovicidal activity of mahlab, Prunus mahaleb L. kernel oil against cotton leafworm, Spodoptera littoralis (Boisd.). The chemical constituents of the fixed oil of mahlab were analyzed using gas-liquid chromatography (GLC). Timnodonic (33.07%), oleic (28.71%) and linoleic (24.35%) were the basic fatty acids, while the major hydrocarbon and sterol were found to be heneicosane (62.57%) and β-sitosterol (10.57%). The LC50 values for the one-day-old egg masses were found to be more susceptible than 3-day-old ones. Moreover, the leaf dip technique occurred to be more efficient than spraying technique. The results also showed abnormalities in the external morphology of egg shell, chorion surface, shell imprints and aeropyles of S. littoralis eggs treated with mahlab and KZ oils as compared to a control by using scanning electron microscope. Generally, the tested oils significantly reduced the activities of transaminase enzymes (AST and ALT), acid and alkaline phosphatases and total soluble protein except mahlab oil on acid phosphatase as compared to a control. Additionally, the oils of both mahlab and KZ oil affected some biological aspects such as incubation period, larval duration, larval mortality and pupal weight comparing to a control.
Źródło:
Journal of Plant Protection Research; 2016, 56, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Systemic protection against pearl millet downy mildew disease induced by cell wall glucan elicitors from Trichoderma hamatum UOM 13
Autorzy:
Lavanya, S.N.
Niranjan-Raj, S.
Nayaka, S.C.
Amruthesh, K.N.
Powiązania:
https://bibliotekanauki.pl/articles/65379.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant protection
systemic protection
pearl millet
downy mildew
plant disease
cell wall glucan
elicitor
Trichoderma hamatum
defense protein
Opis:
The obligate oomycete Sclerospora graminicola (Sacc.) Schroet, is the incitant of downy mildew disease, which is the main constraint in pearl millet production worldwide. Different elicitors from Trichoderma hamatum UOM 13, e.g. mycelial extract and cell wall glucans, were assessed for their resistance elicitation efficiency and the possible underlying mechanisms. Both mycelial extract and cell wall glucans of T. hamatum UOM 13 positively influenced seed quality parameters of pearl millet, significantly enhanced seed germination and seedling vigor in comparison to the untreated control. Seed priming with cell wall glucan elicitors of T. hamatum UOM 13 suppressed downy mildew on susceptible pearl millet seedlings under greenhouse conditions by induction of systemic host resistance. Of the different elicitor delivery methods tested, transplant root dip was more effective than seed treatment and foliar spray. A combination of transplant root dip + seed treatment + foliar spray was significantly more effective than the single delivery methods. The induced resistance corresponded to up regulation of genes of important defense proteins upon pathogen inoculation. Transcripts of genes of defense enzymes glucanase, phenylalanine ammonia lyase, peroxidase and polyphenoloxidase were significantly increased due to the T. hamatum UOM elicitor effect. Expression of hydroxyproline-rich glycoprotein genes, known to play an important role in cell wall cross-linking, were also up regulated in response to T. hamatum UOM cell wall glucan treatment. This study emphasizes the role of T. hamatum UOM as a potential elicitor of downy mildew resistance in pearl millet and presents novel insights into the involvement of important defense proteins mediating such as resistance trigger.
Źródło:
Journal of Plant Protection Research; 2017, 57, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Formulations of Bacillus Spp. and Pseudomonas Fluorescens for Biocontrol of Cantaloupe Root Rot Caused by Fusarium Solani
Autorzy:
Sallam, N.A.
Riad, S.N.
Mohamed, M.S.
El-eslam, A.S.
Powiązania:
https://bibliotekanauki.pl/articles/65978.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to evaluate the different carrier formulations of antagonistic bacteria on incidence of root rot disease of cantaloupe. Twenty-seven isolates of bacteria isolated from rizosphere cantaloupe plants (collected from different localities of the Assiut Governorate, Egypt) were tested in vitro against the growth of Fusarium solani. The tested isolates exhibited varied percentages of mycelial inhibition of F. solani. The highly antagonistic bacteria isolates were identified as Bacillus subtilis, Bacillus cereus, and Pseudomonas fluorescens. The effect of talc based powder and wood flour as various carrier formulations of antagonistic bacteria were tested on incidence of cantaloupe root rot disease in greenhouse and field experiments. All tested carrier formulations of antagonistic bacteria significantly decreased the disease index percentage (p > 0.05) of root rot disease compared with the control, in greenhouse or in field experiments. Application of the wood flour formulation to the infested soil at the time of planting, gave the lowest disease (21.75%) index percentage compared to an application fifteen days before planting (26.83%). The reverse effect occurred in the case of the talc based powder formulation application. In field experiments, during the two growing seasons of 2009 and 2010, wood flour formulation gave the same effect in the reduction of the disease index when added before planting or at the time of planting to soil infested with the pathogen. However, application of the talc formulation at the time of planting showed the least disease index compared to when it was applied fifteen days before planting. In general, wood flour formulation significantly decreased the disease index when compared with the talc formulation. In all the formulations, a number of viable colonies of bioagents were decreased gradually by prolonging the storage time at 4°C. Storage time was prolonged up to five months. But in the case of B. subtilis on talc and B. cereus on wood flour formulations, storage time needed to be prolonged up to seven months.
Źródło:
Journal of Plant Protection Research; 2013, 53, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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