Autor: Mohammadi, M. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Identification and characterization of Pseudomonas syringae pv. syringae strains from various plants and geographical regions
Autorzy:: Khezri, M.
Mohammadi, M.
Powiązania:: https://bibliotekanauki.pl/articles/65669.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: identification
Pseudomonas syringae pv.syringae
plant
phenotype
polymerase chain reaction
protein profile
plasmid
syringomycin
geographic region
Opis:: Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.
Źródło:: Journal of Plant Protection Research; 2018, 58, 4
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Biocontrol potential of Trichoderma harzianum in controlling wilt disease of pistachio caused by Verticillium dahliae
Autorzy:: Fotoohiyan, Z.
Rezaee, S.
Bonjar, G.H.S.
Mohammadi, A.H.
Moradi, M.
Powiązania:: https://bibliotekanauki.pl/articles/65568.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: biological control
Trichoderma harzianum
controlling
wilt disease
antagonism
pistachio
orchard
Verticillium dahliae
Iran
Opis:: Verticillium wilt caused by Verticillium dahliae, is one of the most devastating diseases in pistachio orchards in the world including Iran. In search for an eff ective non-chemical strategy for the management of this disease, we evaluated the biocontrol potential of Trichoderma harzianum isolates obtained from the rhizosphere of healthy pistachio trees in diff erent locations of the Kerman province of Iran against V. dahliae under laboratory and greenhouse conditions. Dual culture tests in the laboratory were conducted in a completely randomized design using 72 T. harzianum isolates. Twenty isolates showed the highest in vitro antagonistic activity. Th e results indicated that all 20 isolates were capable of inhibiting the mycelial growth of V. dahliae signifi cantly. Among them, isolates Tr8 and Tr19 were the most eff ective by 88.89% and 85.12% inhibition, respectively. Extracted cell free metabolites of all eff ective isolates also inhibited the growth of V. dahliae in the culture medium signifi cantly. According to the results, isolates Tr4 and Tr6 inhibited fungal pathogen growth by 94.94% and 88.15% respectively, through production of non-volatile metabolites. In the evaluation of volatile metabolites, isolates Tr5 and Tr4 were the most eff ective by 26.27% and 24.49% growth inhibition, respectively. Based on the results of the in vitro experiments, the fi ve most eff ective isolates were selected for evaluation under greenhouse conditions for their biocontrol potential in controlling Verticillium wilt of pistachio. Results of the greenhouse, (in vivo) experiments were positive and indicated that the occurrence of wilt disease in plants treated with the antagonists alone or in combination with pathogenic fungus was lower than in plants inoculated with pathogen alone. Th e overall results of this study suggest that Trichoderma fungal antagonist may be an eff ective biocontrol agent for the control of Verticillium wilt of pistachio.
Źródło:: Journal of Plant Protection Research; 2017, 57, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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