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Tytuł:
Prevalence of races and biotypes of Ralstonia solanacearum in India
Autorzy:
Chandrashekara, K.N.
Prasannakumar, M.K.
Deepa, M.
Vani, A.
Khan, A.N.A.
Powiązania:
https://bibliotekanauki.pl/articles/65679.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Bacterial wilt caused by Ralstonia solanacearum is the most destructive disease of plants. Fifty-seven isolates of R. solanacearum causing wilt on different host plants viz., tomato (Solanum lycopersicum), brinjal (S. melongena), potato (S. tuberosum), bird of paradise (Strelitzia reginae), ginger (Zingiber officinale), chili (Capsicum annuum), capsicum (Capsicum annuum), davana (Artemisia pallens) and coleus (Coleus forskohlii) were collected from the different agro climatic zones of Karnataka and other parts of India. In this study, 57 isolates were differentiated into race on the basis of their pathogenicity and their ability to infect different host. The isolates were established as race-1. None of the isolates infected mulberry and banana. Fifty-four isolates oxidized and utilized both the disaccharides and sugar alcohols. These isolates were positioned as biovars-3 according to Haywards classification system. Three isolates from Kerala, two ginger, and one tomato strain were not able to utilize dulcitol and lactose. Hence, they were categorized into a new taxo group within the system and designated as biovar-3B for the first time in India. There were 54 isolates which were confirmed as race-1, biovar-3, and 3 isolates were confirmed as race-1, biovar-3B by morphological, physiological, biochemical and pathogenicity studies. Two sets of primers (OLI1 & Y2 and Y1 & Y2) were used in this study to authenticate the organism. Furthermore, the identity of the isolates was confirmed by a serological diagnostic kit obtained from the International Potato Research Center, Lima, Peru, and single chain variable fragment antibody specific to R. solanacearum.
Źródło:
Journal of Plant Protection Research; 2012, 52, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The impact of insecticides on the cotton mealybug, Phenacoccus solenopsis (Tinsley): Efficacy on potato, a new record of host plant in Egypt
Autorzy:
Rezk, M.
Hassan, A.-N.T.
El-Deeb, M.F.
Shaarawy, N.
Dewer, Y.
Powiązania:
https://bibliotekanauki.pl/articles/66067.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The cotton mealybug, Phenacoccus solenopsis (Tinsley) (Hemiptera: Pseudococcidae), has become a widespread pest causing serious losses in several economically important crops, particularly cotton. To the best of our knowledge this is the first record of cotton mealybug, P. solenopsis as a new pest of potato plants in Egypt. The insect was noticed on potato plants for the first time during the growing season of 2016 (mid-August 2016). Mealybug specimens were collected from infested potato plants and identified as P. solenopsis. In an attempt to control this insect pest species, seven insecticides viz. sulfoxaflor, abamectin + thiamethoxam, spirotetramat, thiamethoxam, imidacloprid, buprofezin, and pymetrozine, belonging to different chemical groups, were tested for their effect against nymphs and adult females of P. solenopsis on potato under field conditions. The obtained results indicated that sulfoxaflor, abamectin + thiamethoxam and spirotetramat had the highest efficacy against P. solenopsis recording 80.3–96.05% reduction of the insect population after 21 days of application. Thiamethoxam, imidacloprid, buprofezin and pymetrozine failed to exhibit sufficient P. solenopsis control.
Źródło:
Journal of Plant Protection Research; 2019, 59, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
How do Mentha plants induce resistance against Tetranychus urticae (Acari: Tetranychidae) in organic farming?
Autorzy:
Allam, S.F.
Soudy, B.A.-N.
Hassan, A.S.
Ramadan, M.M.
Baker, D.A.
Powiązania:
https://bibliotekanauki.pl/articles/65704.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Mentha
Mentha piperita
Mentha viridis
spearmint
garden mint zob.spearmint
common mint zob.spearmint
lamb mint zob.spearmint
mackerel mint zob.spearmint
plant resistance
ecology
high performance liquid chromatography
Tetranychus urticae
Acari
Tetranychidae
Opis:
Tetranychus urticae (Acari: Tetranychidae) infesting many plants but Mentha viridis L., and Mentha piperita L., were low in number of infestation. Therefore the objective of this study was to identify the resistance of M. viridis and M. piperita plants against T. urticae by studying the external shape and internal contents of those plants. For morphological studies, dried leaves were covered with gold utilizing an Edwards Scan coat six sputter-coater. For histological studies, arrangements of Soft Tissue technique were used. For phytochemical studies, the plants were cut, dried and then high performance liquid chromatography (HPLC) was used. While feeding the mites were collected from the area between oily glands, trichomes and respiratory stomata in both mint species. The most important leaf structures in aromatic plants are the oily glands found on the external part of the leaves (both upper and lower epidermis). The number of oil glands in M. viridis leaves was greater than in M. piperita; the trichomes on the epidermis of M. viridis were greater in number than in M. piperita; the spongy mesophyll in M. viridis was much thicker than in M. piperita. The essential oils in the leaves of both mint species contained 71 compounds representing 99.61% of the total oil constituents identified from M. viridis before infestation, and 90.95% after infestation, and about 99.65% from M. piperita before infestation, and 99.98% after infestation.
Źródło:
Journal of Plant Protection Research; 2018, 58, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A study on controlling Setaria viridis and Corchorus olitorius associated with Phaseolus vulgaris growth using natural extracts of Chenopodium album
Autorzy:
El-Rokiek, K.G.
El-Din, S.A.S.
Shehata, A.N.
El-Sawi, S.A.M.
Powiązania:
https://bibliotekanauki.pl/articles/66191.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
controlling
Setaria viridis
Corchorus olitorius
Phaseolus vulgaris
growth
leaf extract
Chenopodium album
bean
flavonoids
polyphenol
root extract
plant extract
Opis:
The effects of water extracts of Chenopodium album leaves and roots on the growth of grass weed (Setaria viridis) and broad leaf weed (Corchorus olitorius) grown with beans (Phaseolus vulgaris) in greenhouse pots were studied in the National Research Centre, Giza, Egypt. In this experiment fresh leaf and root extracts and their corresponding dry leaf and root extracts at different concentrations were used. There were significant inhibitions in the dry weights of S. viridis and C. olitorius by all extracts at the flowering stage of beans and at harvest. The inhibition effect of all C. album extracts on both weeds (dry weight/pot) depended on the extracted plant organ (leaf or root), its fresh or dry form as well as its concentrations. The inhibition caused by the leaf extract was much higher on weed growth than that of root extract. A higher concentration of fresh leaf extract (25%) had the highest significant inhibition effect. The results also indicated that C. olitorius was more sensitive to the extracts than S. viridis. On the other hand, the inhibition effect of the extracts on the growth of both weeds was accompanied by increased bean growth and yield/plant. The analysis of both leaf and root extracts of C. album revealed that the total content of polyphenols and flavonoids in the leaf extract was more than triple that of the root extract. The results suggested that the fresh leaf extract of C. album may be a possible tool for the development of weed control using natural herbicides.
Źródło:
Journal of Plant Protection Research; 2016, 56, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification and diversity of Fusarium species isolated from tomato fruits
Autorzy:
Murad, N.B.A.
Kusai, N.A.
Zainudin, N.A.I.M.
Powiązania:
https://bibliotekanauki.pl/articles/66221.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
identification
diversity
Fusarium oxysporum
Gibberella fujikuroi
isolate
tomato fruit
Opis:
Fruit rot of tomato is a serious disease caused by Fusarium species. Sampling was conducted throughout Selangor, Malaysia and fungal species identification was conducted based on morphological and gene encoding translation elongation factor 1-α (tef1-α) sequence analysis. Five species of Fusarium were discovered namely F. oxysporum (including F. oxysporum f. sp. lycopersici), F. solani, F. equiseti, F. proliferatum and F. verticillioides. Our results provide additional information regarding the diversity of Fusarium species associated with fruit rot disease of tomato.
Źródło:
Journal of Plant Protection Research; 2016, 56, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Semiochemicals for controlling insect pests
Autorzy:
El-Ghany, N.M.A.
Powiązania:
https://bibliotekanauki.pl/articles/65410.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Semiochemicals are defined as informative molecules mainly used in plant-insect or insectinsect interactions as alternative or complementary components to insecticide approaches in different integrated pest management strategies. They are used to manipulate insect behaviour by affecting the survival and/or reproduction of insect pests for controlling their infestations on crops. The present review provides a basic summary of the utilization of semiochemicals for controlling insect pests. Two main topics were explored in this study. The first topic focuses on a description of semiochemicals and their types (pheromones and allelochemicals). Pheromones represent an intraspecific communication amidst members of the same species. Allelochemicals, produced by individuals of one species, modify the behavior of individuals of a different species (i.e. an interspecific effect). Allelochemicals include different informative molecules such as: allomones, kairomones, synomones, antimones and apneumones. The second topic focuses on the application of semiochemicals in IPM programs. Different semiochemicals are included in integrated pest management programs in various ways such as monitoring, mass trapping, attract-and-kill, push-pull, and disruption strategies. Pheromones are promising and can be used singly or in integration with other control strategies for monitoring and controlling insect pests in agricultural systems. For example, sex pheromones have been applied in mass trapping, disruption and attract-and-kill tactics in IPM programs.
Źródło:
Journal of Plant Protection Research; 2019, 59, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Formulations of Bacillus Spp. and Pseudomonas Fluorescens for Biocontrol of Cantaloupe Root Rot Caused by Fusarium Solani
Autorzy:
Sallam, N.A.
Riad, S.N.
Mohamed, M.S.
El-eslam, A.S.
Powiązania:
https://bibliotekanauki.pl/articles/65978.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to evaluate the different carrier formulations of antagonistic bacteria on incidence of root rot disease of cantaloupe. Twenty-seven isolates of bacteria isolated from rizosphere cantaloupe plants (collected from different localities of the Assiut Governorate, Egypt) were tested in vitro against the growth of Fusarium solani. The tested isolates exhibited varied percentages of mycelial inhibition of F. solani. The highly antagonistic bacteria isolates were identified as Bacillus subtilis, Bacillus cereus, and Pseudomonas fluorescens. The effect of talc based powder and wood flour as various carrier formulations of antagonistic bacteria were tested on incidence of cantaloupe root rot disease in greenhouse and field experiments. All tested carrier formulations of antagonistic bacteria significantly decreased the disease index percentage (p > 0.05) of root rot disease compared with the control, in greenhouse or in field experiments. Application of the wood flour formulation to the infested soil at the time of planting, gave the lowest disease (21.75%) index percentage compared to an application fifteen days before planting (26.83%). The reverse effect occurred in the case of the talc based powder formulation application. In field experiments, during the two growing seasons of 2009 and 2010, wood flour formulation gave the same effect in the reduction of the disease index when added before planting or at the time of planting to soil infested with the pathogen. However, application of the talc formulation at the time of planting showed the least disease index compared to when it was applied fifteen days before planting. In general, wood flour formulation significantly decreased the disease index when compared with the talc formulation. In all the formulations, a number of viable colonies of bioagents were decreased gradually by prolonging the storage time at 4°C. Storage time was prolonged up to five months. But in the case of B. subtilis on talc and B. cereus on wood flour formulations, storage time needed to be prolonged up to seven months.
Źródło:
Journal of Plant Protection Research; 2013, 53, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The integration between Trichogramma evanescens west. (Hymenoptera: Trichogrammatide) and selected bioinsecticides for controlling the potato tuber moth Phthorimaea operculella (Zell.) (Lepidoptera: Gelechiidae) of stored potatoes
Autorzy:
Mandour, N.S.
Sarhan, A.A.
Atwa, D.H.
Powiązania:
https://bibliotekanauki.pl/articles/66835.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The efficacy of the egg-egg parasitoid Trichogramma evanescens (Hymenoptera: Trichogrammatidae) and certain bioinsecticides (e.g., Neemix, Virotecto, Agerin, Dipel 2x and Spinosad) for controlling the potato tuber moth (PTM), Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) under storage conditions was studied. Single and combined treatments of T. evanescens and the bioinsecticides were tested. Neemix and spinosad were evaluated as spray treatments. Virotecto, Agerin and Dipel 2x were evaluated as dust and spray treatments. Data were recorded in terms of rate of infestation, reduction of infestation, number of P. operculella pupae, and number of mines per 20 tubers as well as percentage of edible parts. Data revealed that the percentage of infestation in the control treatment was as high as in the Neemix treatment being 96.67 and 90% after one and two months of storage, respectively. Obviously, Spinosad and Dipel 2x were the most effective bioinsecticides in reducing tuber infestation and number of P. operculella recovered pupae. Virotecto and Agerin reduced rate of infestation and number of recovered P. operculella pupae over that of the control, but their effect was significantly lower than that of Spinosad or Dipel 2x. Moreover, there were significant differences among treatments in the form of the number of P. operculella tunnels and percentages of edible parts after two months in storage. Data further indicated that the integration between the tested bioinsecticides and T. evanescens enhanced the control of P. operculella. Significant differences in percentages of infestation, edible parts, number of recovered pupae as well as number of mines in stored potatoes existed between individual treatments (without Trichogramma) and combined treatments.
Źródło:
Journal of Plant Protection Research; 2012, 52, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of different constant temperatures on biology of Schizaphis graminum (rondani) (Hemiptera: aphididae) on barley, Hordeum vulgare L. (poaceae) in Iran
Autorzy:
Tofangsazi, N.
Kheradmand, K.
Shahrokhi, S.
Talebi, A.A.
Powiązania:
https://bibliotekanauki.pl/articles/65026.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The temperature dependent biology of greenbug, Schizaphis graminum Rondani on Kavir barley cultivar was studied at seven constant temperatures including 10, 15, 19, 22, 26, 31, and 33±1°C, 70% relative humidity (RH), and a photoperiod of 16:8 (L:D) hours. The period of immature development ranged between 6.60 days at 26°C to 28.56 days at 10°C, respectively. All tested aphids failed to develop at 33°C. The calculated rm and λ values were significantly the highest at 26°C and lowest at 10°C, respectively. The mean generation time and doubling time of S. graminum decreased linearly by increasing the temperature from 10 to 26°C. Additionally, the total number of offsprings per female was extremely low at 10 and 31°C, contrary to the highest and lowest values of life expectancy at 10°C (41.73 days) and 31°C (7.66 days), respectively. The results of the present study revealed that temperature had great effects on biology of S. graminum, which was never previously studied on barley in Iran.
Źródło:
Journal of Plant Protection Research; 2012, 52, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antifungal activities of bacteria producing bioactive compounds isolated from rice phyllosphere against Pyricularia oryzae
Autorzy:
Wiraswati, M.N.
Rusmana, I.
Nawangsih, A.A.
Wahyudi, A.T.
Powiązania:
https://bibliotekanauki.pl/articles/65341.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Rice blast is the main disease of rice plants in Indonesia and several countries worldwide. Controlling this disease using chemical fungicides has harmful effects on the environment. Therefore, we need biocontrol agents which are more environmentally friendly such as rice phyllosphere bacteria. This study aimed to explore bacteria producing bioactive compounds from the rice phyllosphere environment to control blast disease. A total of 88 isolates were successfully isolated from rice leaves in Sukabumi, Situgede, and Jasinga (West Java, Indonesia). From them, we obtained 22 bacteria isolates with antifungal activity against Pyricularia oryzae in vitro assay. In addition, seven non-pathogenic bacteria were obtained from further screening in hypersensitivity, hemolysis and pathogenicity assays, namely STGG 3, STGG 7, STGG 8, STGG 14, SKBV 1, STGV 8, and SKBG 78. To show their antifungal activity, we tested crude extracts of these seven isolates and the results revealed that all the crude extracts can inhibit the growth of P. oryzae. Based on a genetic approach, isolates STGG 3, STGG 7, and STGG 14 were found to have both nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) genes, while isolate SKBV 1 only had the NRPS gene. The NRPS and PKS genes from potential isolates were similar to NRPS and PKS genes of Bacillus sp. in different strains. Furthermore, molecular identification based on the 16S rRNA gene revealed that the seven potential isolates belong to three genera, i.e. Bacillus (STGG 3, STGG 7, STGG 8, STGG 14, SKBV 1), Enterobacter (STGV 8) and Brachybacterium (SKBG 78). We suggest that the seven isolates found in this study have potency and could be recommended as biocontrol agents of blast disease caused by P. oryzae.
Źródło:
Journal of Plant Protection Research; 2019, 59, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modes of transmission and stability of Rice yellow mottle virus
Autorzy:
Uke, A.
Tibanyendela, N.
Ikeda, R.
Fujiie, A.
Natsuaki, K.T.
Powiązania:
https://bibliotekanauki.pl/articles/66531.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Rice yellow mottle virus (RYMV) is the most important rice virus in Africa. We examined RYMV transmission via soil and water contaminated with RYMV-infected rice plants and by serial cutting with RYMV-contaminated scissors. Transmission of RYMV via dried rice straw kept at 27°C was also examined. The results showed the virus could be transmitted via soil and water, and by scissors. Rice straw that was RYMV-infected was not infective if it was dried and was kept longer than 42 days. By insect transmission experiments and ELISA, long-horned grasshoppers (Conocephalus spp.) were found to be a possible vector of RYMV in Uganda.
Źródło:
Journal of Plant Protection Research; 2014, 54, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antimicrobial activity of leaf and flower extracts of Lippia nodiflora L. (Verbenacea)
Autorzy:
Zare, Z.
Majd, A.
Sattari, T.N.
Iranbakhsh, A.
Mehrabian, S.
Powiązania:
https://bibliotekanauki.pl/articles/66574.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antimicrobial activity
leaf
flower
plant extract
Lippia nodiflora
Verbenacea
frog fruit
sawtooth fogfruit zob.frog fruit
Turkey tangle zob.frog fruit
Phyla nodiflora zob.Lippia nodiflora
medicinal plant
bacteria
fungi
Opis:
Antimicrobial activities of the methanolic extracts from the leaves and flowers of Lippia nodiflora L. (Verbenaceae), were studied by the disk diffusion method. The extracts showed antimicrobial impact on bacteria such as Bacillus subtilis, B. cereus, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, K. oxytoca and Esherichia coli as well as fungi such Aspergillus niger and Candida albicanse. The results showed that increasing concentrations of extracts increased the antimicrobial activities in all of the microorganisms. Bacteria were more sensitive than fungi, and gram positive bacteria were more sensitive than gram negative ones.
Źródło:
Journal of Plant Protection Research; 2012, 52, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of some microbial agents, natural and chemical compounds for controlling tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)
Autorzy:
El-Ghany, N.M.A.
Abdel-Razek, A.S.
Ebadah, I.M.A.
Mahmoud, Y.A.
Powiązania:
https://bibliotekanauki.pl/articles/65257.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
microbial agent
natural compound
chemical compound
biopesticide
natural insecticide
controlling
tomato
leaf miner
Tuta absoluta
Lepidoptera
Gelechiidae
Opis:
Solanaceous plants have a great economic impact in Egypt. These groups of plants include potatoes, tomatoes and eggplants. The new invasive pest of tomatoes, Tuta absoluta (Meyrick) causes the greatest crop losses which can range from 60 to 100%. After its detection in Egypt during the last half of 2009, it spread quickly to all provinces in the country. We aiming to propose a sustainable control program for this devastating pest. In this research we tested three groups of control agents. The first was microbial and natural, the second – plant extracts and the third – chemical insecticides. Our results showed that the impact of T. absoluta can be greatly reduced by the use of sustainable control measures represented by different insecticide groups. Bioassay experiments showed that this devastating pest can be controlled with some compounds that give high mortality rates. Of these compounds, spinosad and Beauveria bassiana, microbial control agents, followed by azadirachtin, gave the best results in controlling T. absoluta. Of the chemical insecticides, lambda-cyhalotrin was the most effective, followed by lufenuron and profenofos. In conclusion we encourage farmers to use microbial and natural control measures in combating the tomato leafminer, T. absoluta, in Integrated Pest Mangement (IPM) programs.
Źródło:
Journal of Plant Protection Research; 2016, 56, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Drechslera graminea on total soluble proteins and defense enzymes of barley
Autorzy:
Goel, N.
Jaiswal, G.
Srivastava, A.K.
Paul, P.K.
Goel, A.
Powiązania:
https://bibliotekanauki.pl/articles/66670.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
barley
Hordeum vulgare
defense enzyme
Drechslera graminea
peroxidase
phenylalanine lyase
polyphenol oxidase
protein
soluble protein
tyrosine ammonia lyase
Opis:
Barley phylloplane is seriously colonized by Drechslera graminea, the causal agent of leaf stripe disease in the hos. The present study involved the elucidation of alterations induced in the protein content of the host due to Drechslera infection. Naturally growing barley plants were obtained from fields and Drechslera graminea was isolated and identified from diseased plants’ leaves. After identification and preparation of the pure culture, the pathogen was inoculated on plants grown under aseptic and controlled laboratory conditions. Changes in the total soluble cytoplasmic proteins and defense enzymes of the host such as polyphenol oxidase (PPO), peroxidase (POX), phenylalanine lyase (PAL) and tyrosine ammonia lyase (TAL) were observed up to 5 h after inoculation. The results demonstrated a significant effect of the pathogen on the cytoplasmic protein expression of the host as well as in its defense system.
Źródło:
Journal of Plant Protection Research; 2018, 58, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of cereal cyst nematodes (Heterodera spp.) in Morocco based on morphology, morphometrics and rDNA-ITS sequence analysis
Autorzy:
Mokrini, F.
Viaene, N.
Waeyenberge, L.
Dababat, A.A.
Moens, M.
Powiązania:
https://bibliotekanauki.pl/articles/65431.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cereal cyst nematode
nematode
Heterodera avenae
Heterodera latipons
Heterodera filipjevi
morphology
morphometry
Internal transcribed spacer
rDNA
sequence analysis
Morocco
Opis:
Morphological and molecular diversity among 11 populations of cereal cyst nematodes from different wheat production areas in Morocco was investigated using light microscopy, species-specific primers, complemented by the ITS-rDNA sequences. Morphometrics of cysts and second-stage juveniles (J2s) were generally within the expected ranges for Heterodera avenae; only the isolate from Aïn Jmaa showed morphometrics conforming to those of H. latipons. When using species-specific primers for H. avenae and H. latipons, the specific bands of 109 bp and 204 bp, respectively, confirmed the morphological identification. In addition, the internal transcribed spacer (ITS) regions were sequenced to study the diversity of the 11 populations. These sequences were compared with those of Heterodera species available in the GenBank database (www.ncbi.nlm.nih.gov) and confirmed again the identity of the species. Ten sequences of the ITS-rDNA were similar (99–100%) to the sequences of H. avenae published in GenBank and three sequences, corresponding with one population, were similar (97–99%) to H. latipons.
Źródło:
Journal of Plant Protection Research; 2017, 57, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł

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