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Wyświetlanie 1-15 z 18
Tytuł:
Detection of bovine leucocyte adhesion deficiency [BLAD] carriers using a new PCR test
Autorzy:
Kaminski, S
Czarnik, U
Powiązania:
https://bibliotekanauki.pl/articles/2046599.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cattle
bovine leucocyte adhesion deficiency
polymerase chain reaction
bacterial infection
Opis:
In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non-random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.
Źródło:
Journal of Applied Genetics; 1997, 38, 1; 51-55
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Familial polyposis coli - inducing mutations in APC gene in Poland
Autorzy:
Pawlak, A L
Plawski, A
Smoczkiewicz, P
Kwiatkowska, J
Meissner, W
Krokowicz, P
West, S P
Powiązania:
https://bibliotekanauki.pl/articles/2046600.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
familial adenomatous polyposis
protein truncation test
Polish population
mutation
polymerase chain reaction
heteroduplex analysis
Opis:
Screening for molecular changes within the adenomatous polyposis coli (APC) gene, exons 11-14 and the 5’ half of exon 15, encompassing the mutation cluster region within exon 15, was performed in 30 patients with Familial Polyposis Coli (FAP). All patients were studied by heteroduplex analysis (HA) and single strand conformation polymorphism (SSCP) and molecular changes were found in 7 cases. Protein truncation test (PTT) has been performed in 17 cases in which mutations have not been found earlier, and shortening of protein product was noted in 2 cases. In three cases common deletion of 5 bp at codon 1309 and in one 5 bp deletion at codon 1061 were found. In other cases the molecular changes were demonstrated as heteroduplexes in exon 14 (1 patient), in segments E and F (one patient each) of exon 15, and in two cases the heteroduplexes were within the overlapping sequences of segments E/F and F/G of exon 15, respectively. In families where the molecular changes were found by HA, 7 persons at high risk for FAP were found and advised to undergo regular endoscopic examinations. In three persons at risk the transfer of mutation was excluded.
Źródło:
Journal of Applied Genetics; 1997, 38, 1; 77-85
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adaptor-mediated amplification of minute amounts of severely fragmented ancient nucleic acids
Autorzy:
Pusch, C M
Blin, N.
Broghammer, M.
Nicholson, G.J.
Scholz, M.
Powiązania:
https://bibliotekanauki.pl/articles/2042022.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mitochondrial DNA
genetics
nucleic acid
ancient DNA
polymerase chain reaction
DNA
cDNA
sex typing
Źródło:
Journal of Applied Genetics; 2000, 41, 4; 303-315
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use amelogenin gene polymorphism in PCR embryo sexing in bovine IVF embryos
Autorzy:
Lechniak, D
Cumming, J R
Powiązania:
https://bibliotekanauki.pl/articles/2046605.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
polymorphism
sex chromosome
Y chromosome
bovine embryo
sex
amelogenin gene
polymerase chain reaction
embryo
Opis:
The present study describes a rapid, simple method of bovine IVF embryo sexing by use of PCR technique. A pair of primers corresponding to the bovine amelogenin sequence has been used. The Rapid Cycler (Idaho Technology, USA) used in the current experiment enabled the PCR programme consisting of 55 cycles to be completed in less than 40 minutes. Therefore the total sexing procedure could be performed in less than 90 minutes. The described method succeded in case of 85% analysed embryos.
Źródło:
Journal of Applied Genetics; 1997, 38, 1; 45-49
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Large-scale analysis of sequence tags in Xp11.4-11.3 and evaluation of candidate genes for X-linked ocular diseases
Autorzy:
Pusch, C M
Powiązania:
https://bibliotekanauki.pl/articles/2041943.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
X chromosome
man
pseudogene
polymerase chain reaction
X-linked ocular disease
night vision
genetic disease
Źródło:
Journal of Applied Genetics; 2001, 42, 1; 89-102
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The quantitative PCR technique resolves ambiguities concerning a small rearrangement of human chromosome 6q12-13
Autorzy:
Nowacka, J
Helszer, Z.
Walter, Z.
Plucienniczak, A.
Kaluzewski, B.
Powiązania:
https://bibliotekanauki.pl/articles/2048327.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
duplication
chromosome
human chromosome
man
rearrangement
cytogenetic diagnosis
karyotype analysis
chromosome aberration
polymerase chain reaction
inversion
Źródło:
Journal of Applied Genetics; 2001, 42, 4; 541-545
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Association of arylamine N-acetyltransferase [NAT1 and NAT2] genotypes with urinary bladder cancer risk
Autorzy:
Jaskula-Sztul, R
Sokolowski, W.
Gajecka, M.
Szyfter, K.
Powiązania:
https://bibliotekanauki.pl/articles/2041980.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
tobacco smoking
human genetics
genotype
urinary bladder
polymerase chain reaction
cancer
acetylator genotype
arylamine N-acetyltransferase
Źródło:
Journal of Applied Genetics; 2001, 42, 2; 223-231
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Unidirectional orientation of twelve expressed tagged sites within 4o kb of human chromosomal region 22q13.1
Autorzy:
Pusch, C
Wang, Z.
Roe, B.
Blin, N.
Powiązania:
https://bibliotekanauki.pl/articles/2048293.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
RNA
human chromosome
chromosome
hybridization
genetic change
linear map
polymerase chain reaction
DNA
cosmid
cDNA
transcriptional orientation
Opis:
The single copy sequence D22S16 from human chromosomal region 22q13.1 that carries a putative conserved gene, was used to probe a chromosome 22-specific cosmid library. Genomic sequencing of one positive, 40 kb long cosmid (C1155) revealed a hereto unmapped gene (a subunit of DNA-dependent RNA polymerase II, POLR2F), a SOX9-related sequence and 12 expressed sequence tags. Although not parts of one consecutive gene, all 12 ESTs and, in addition, the polymerase gene are oriented in the same transcriptional direction within the genomic sequence represented by cosmid C1155.
Źródło:
Journal of Applied Genetics; 1998, 39, 2; 199-204
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotyping of bovine beta-lactoglobulin [LGB] by PCR-SSCP technique
Autorzy:
Kaminski, S
Zabolewicz, T
Powiązania:
https://bibliotekanauki.pl/articles/2046615.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
AB genotype
SSCP method
cattle
genotype
AA genotype
BB genotype
beta-lactoglobulin
polymerase chain reaction
allele
identification
Opis:
A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 471-476
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Length polymorphism of PCR-amplified genomic fragments of the pregnancy-associated glycoprotein [PAG] gene family in the pig and some other domestic and wild mammals
Autorzy:
Szafranska, B
Panasiewicz, G.
Waclawik, A.
Powiązania:
https://bibliotekanauki.pl/articles/2041811.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
trophectoderm
pregnancy
pig
polymorphism
mammal
chorion
domestic mammal
polymerase chain reaction
wild mammal
pregnancy-associated glycoprotein gene
Źródło:
Journal of Applied Genetics; 2001, 42, 3; 335-349
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA polymorphism in locus D1S80 in Poland. DNA profiling and detection of new alleles by heteroduplex formation between alleles of the same size
Autorzy:
Kwiatkowska, J
Dziechciowska, K
Lisiecka, D
Slomski, R
Powiązania:
https://bibliotekanauki.pl/articles/2046677.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
DNA sequence
polymorphism
Polska
hybridization
Polish population
heteroduplex formation
DNA polymorphism
same size
polymerase chain reaction
allele
distribution
Opis:
We have analysed allele distribution at the highly polymorphic variable number of tandem repeats (VNTR) locus D1S80 (pMCT118) in the Polish population using the, polymerase chain reaction (PCR) technique. Characteristics of the D1S80 locus makes it a very useful marker for population genetic research, genetic linkage studies and forensic identification of individuals. During our routine application of the D1S80 marker to paternity testing in several cases of homozygosity detected by polyacrylamide gel electrophoresis, heteroduplex formation for alleles 18 and 24 was also observed. Direct sequencing of PCR products revealed that alleles 18 and 24 of locus D1S80 actually represent a mixture composed of different sequences. Our observations indicate that identification of some 18 and 24 VNTR alleles based only on size estimated in electrophoretic analyses could lead to errors in paternity testing and DNA profiling.
Źródło:
Journal of Applied Genetics; 1997, 38, 3; 335-341
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]
Autorzy:
Sadowski, J
Rorat, T
Cooke, R
Delseny, M
Powiązania:
https://bibliotekanauki.pl/articles/2046684.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
beta-amylase
rye
gene promoter
homology
amino acid
DNA cloning
prolamin
Secale cereale
mRNA
embryogenesis
endosperm
cDNA
inverse polymerase chain reaction
Opis:
The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.
Źródło:
Journal of Applied Genetics; 1997, 38, 3; 241-251
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Homology of DNA sequences encompassing the malignant hyperthermia mutation site in the human, porcine, and zebrine ryrl gene
Autorzy:
Gronek, P
Slomski, R.
Lisiecka, D.
Plawski, A.
Nuc, K.
Banasiewicz, T.
Powiązania:
https://bibliotekanauki.pl/articles/2044249.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
homology
Equus grevyi
Sus scrofa
ryanodine receptor
chromosome
polymorphism
gene
porcine gene
man
mutation
polymerase chain reaction
DNA
malignant hyperthermia
skeletal muscle
Opis:
The RYR1 gene encoding the Ca²⁺ channel of sarcoplasmic reticulum of human skeletal muscle has been cloned and its nucleotide sequence has been determined earlier. We have used the polymerase chain reaction single strand conformation polymorphism (PCR-SSCP), and sequencing analysis for human, porcine (Sus scrofa), and zebrine (Equus grevyi) ryanodine receptor (ryrl) gene. The fragment of exon 17 of the ryr1 gene was characterized by a high homology between all the analysed species (substitution of a nucleotide is underlined): porcine ryr1 ¹⁸³⁴GTG GCC GTG CGC TCC AAC CAA GAT CT¹⁸⁵⁹ human RYR1 ¹⁸³¹GTG GCC GTG CGC TCC AAC CAA GAT CT¹⁸⁵⁶ zebrine ryr1 GTG GCC GTG CGC TCC AAC CAA GAC CT.
Źródło:
Journal of Applied Genetics; 1998, 39, 3; 275-279
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of random amplified polymorphic DNA [RAPD] assay for differentiation among isolates of Stagonospora spp. and Septoria tritici
Autorzy:
Czembor, P C
Arseniuk, E
Powiązania:
https://bibliotekanauki.pl/articles/2047271.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Stagonospora
Stagonospora avenae
Septoria tritici
random amplified polymorphic DNA
pathogen
polymerase chain reaction
DNA
molecular marker
fungal isolate
Stagonospora nodorum
genetic variation
Opis:
The genetic similarity of three species: Septoria tritici, Stagonospora nodorum and Stagonospora avenae f. sp. triticea - important pathogens in many cereal production areas worldwide was assessed by random amplified polymorphic DNA (RAPD) assay. In preliminary research DNA of 14, 9, and 7 monopyenidios- pore isolates of S. nodorum, S. tritici, and S. a. tritícea, respectively, were amplified by PCR with four primers. Afterwards the research was focused on three mono- pyenidiospore isolates from each species studied. The isolates of each species selected for the study varied in pathogenicity and were diverse geographically. PCR with the set of 14 selected primers resulted in 99 different bands, ranged from 180 to 2500 base pairs in length. Most primers in PCR (especially RAD11, RAD31, RAD32, RAD33) revealed uniform bands for isolates of S. a. tritícea, that allow to identify this species among the others. The cluster analysis using Unweighed Pair-Group Method with Averaging (UPGMA) revealed interspecies disagreement among the isolates ranging from 32 to 53%. The intraspecies disagreement ranges were 17-20%, 38-43%, 42-44% for S. avenae f. sp. triticea, S. nodorum and S. tritici, respectively. Cluster analysis classified isolates into three homogeneous clusters. Each cluster grouped isolates of one species according to their current taxonomie ranks based on spore size, colony morphology and host ranges. In addition, two of the clusters represented by isolates of S. nodorum and S. a. tritícea were distinctly separated at a lower linkage distance from the third one comprising isolates of S. tritici. A slight inconsistency found in grouping some isolates indicates that such groupings should be done with caution. The present study indicates that the PCR- RAPD assay is of a potential use in taxonomy of Stagonospora spp. and Septoria tritici as well as in molecular identification of casual disease agents.
Źródło:
Journal of Applied Genetics; 1996, 37, 3; 239-251
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular follow up of donor lymphocyte infusion in CML children after allogeneic bone marrow transplantation
Autorzy:
Ladon, D
Pieczonka, A.
Jolkowska, J.
Wachowiak, J.
Witt, M.
Powiązania:
https://bibliotekanauki.pl/articles/2048295.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
minimal residual disease
in situ
human genetics
bone marrow
hybridization
fluorescence
donor lymphocyte infusion
chronic myeloid leukemia
child
polymerase chain reaction
transplantation
Źródło:
Journal of Applied Genetics; 2001, 42, 4; 547-552
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 18

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