- Tytuł:
- Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]
- Autorzy:
-
Sadowski, J
Rorat, T
Cooke, R
Delseny, M - Powiązania:
- https://bibliotekanauki.pl/articles/2046684.pdf
- Data publikacji:
- 1997
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
beta-amylase
rye
gene promoter
homology
amino acid
DNA cloning
prolamin
Secale cereale
mRNA
embryogenesis
endosperm
cDNA
inverse polymerase chain reaction - Opis:
- The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.
- Źródło:
-
Journal of Applied Genetics; 1997, 38, 3; 241-251
1234-1983 - Pojawia się w:
- Journal of Applied Genetics
- Dostawca treści:
- Biblioteka Nauki
Artykuł