- Tytuł:
- Genetic polymorphism and quantitative variation of alpha-amylases from rye endosperm
- Autorzy:
-
Masojc, P
Lapinski, M.
Larsson-Raznikiewicz, M. - Powiązania:
- https://bibliotekanauki.pl/articles/2048192.pdf
- Data publikacji:
- 1995
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
chromosome
rye
Secale cereale
colorimetric assay
isoenzyme
alpha-amylase
electrophoresis
endosperm
isoelectric focusing
immunoelectrophoresis
cereal cultivar
genetic polymorphism - Opis:
- α-Amylase isozymes from rye endosperm were analysed by means of isoelectric focusing, polyacrylamide gel electrophoresis, immunoelectrophoresis and colorimetric assay. Α-AMY 1 (high pI) group was separated into 13 IEF bands, whereas in group α-AMY2 (low pI) 2 intensive and 6-8 faint bands were found. Two linked (2±1 cM) polymorphic loci and a single locus with two alleles encoding for α-AMY 1 and α-AMY 2 groups, respectively, were identified after genetic analysis of the IEF patterns. All α-amylase isozymes developed on PAGE, were shown to belong to α-AMY 1 group. It was demonstrated that a single PAGE isozyme corresponds to 2-4 separate IEF bands and that most of the IEF bands can be attributed to more than one PAGE isozyme. The activity of α-amylases from PAGE zone I was 2.3 times higher than the activity of zone II isozymes. A strong correlation between the activity and protein amount of particular α-AMY 1 isozymes (r=0.94) was found.
- Źródło:
-
Journal of Applied Genetics; 1995, 36, 3; 197-204
1234-1983 - Pojawia się w:
- Journal of Applied Genetics
- Dostawca treści:
- Biblioteka Nauki
Artykuł