Temat: A2 - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Linkage and interdependence of A2MD1 and A2ME2 alpha2-macroglobulin genes in cattle
Autorzy:: Wegrzyn, J
Skiba, E
Krzyscin, P
Powiązania:: https://bibliotekanauki.pl/articles/2047144.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: antigen marker
gene
animal production
alpha2-macroglobulin
cattle
antigenic determinant
protein
marker
A2MD1 gene
A2ME2 gene
Opis:: The transmission of previously described genes A2MD1 and A2ME2 that determine antigenic markers of alpha₂-macroglobulins A₂mD1 and А₂mЕ2 in cattle was studied. The starting point for the analyses was the lack of individuals negative for both markers in the population of 3551 Black-and-White, Red-and-White, Polish Red and Simmental cattle and interbreed crosses. Controlling of these specificities by allelic genes or genes from closely linked loci was considered. To support or reject this hypothesis, the independence test 2 x 2 and analysis of segregation of A₂mD1 and А₂mЕ2 in the offsprings of all phenotypic matings found and of selected matings in which genotypes of sires were determined, were used. It was found that the observed segregation of antigenic markers in the offsprings rules out the possibility that they are determined by allelic genes. The results obtained show that markers A₂mD1 and А₂mЕ2 are controlled by the genes A2MD1 and A2ME2 from closely linked loci. Moreover it seems that only those haplotypes are transmitted in which both genes - A2MD1 and A2ME2, or one of them - A2MD1 or A2ME2, are present. No haplotypc would then be transmitted (would occur?) in which both genes are in the recessive form. recessive form.
Źródło:: Journal of Applied Genetics; 1997, 38, 2; 187-193
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Molecular studies in osteogenesis imperfecta [OI] II. Evaluation of intragenic polymorphic sites in COL1A1 and COL1A2 loci
Autorzy:: Kostyk, E
Sucharski, P.
Pietrzyk, J.J.
Kruczek, A.
Powiązania:: https://bibliotekanauki.pl/articles/2044212.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: intragenic polymorphic site
polymorphism
haplotype
DNA isolation
COL1A1 gene
electrophoresis
collagen
COL1A2 gene
molecular marker
osteogenesis imperfecta
Opis:: The goal of the study was to evaluate intragenic polymorphic sites in COL1A1 and COL1A2 loci. For COL1A1 the following intragenic markers were used: PCR-RFLP (COL1A1), G/A polymorphism in exon 45 of COL1A1 and C/T polymorphism in +88 position of COL1A1 non-translatable 3’ end. For COL1A2 PCR-VNTR was analyzed. 17 families were examined (6 of the "simplex" type and 11 of the "multiple" type). In 8 out of 11 "multiplex" families the segregation of the markers revealed correlation with OI, whereas the other 3 were non-informative. The method was not useful in "simplex" families.
Źródło:: Journal of Applied Genetics; 1998, 39, 4; 349-365
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Molecular studies in osteogenesis imperfecta [OI] III. cDNA of COL1A1 and COL1A2 analysis using the BESS-T-Scan technique
Autorzy:: Sucharski, P
Sanak, M.
Kostyk, E.
Pietrzyk, J.J.
Kruczek, A.
Powiązania:: https://bibliotekanauki.pl/articles/2044221.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: COL1A1 gene
electrophoresis
COL1A2 gene
collagen production
man
RNA isolation
mutation
BESS-T-Scan technique
molecular diagnosis
DNA
osteogenesis imperfecta
cDNA synthesis
fibroblast culture
Opis:: A BESS-T-Scan analysis of cDNA COL1A1 and COL1A2 obtained by RT-PCR derived from five patients with sporadic forms of ostegenesis imperfecta was performed. The study was done in four patients with type I and one patient with type III OI. The analysis revealed the presence of structural changes in two regions of cDNA COL1A1 in two patients. No quantitative changes referring to COL1A2 gene were noted in any patient. The above analysis was the first application of the BESS-T-Scan technique in a molecular diagnosis of OI. The applied method seems to be useful and fulfil the basic criteria of the screening method to detect and locate mutations.
Źródło:: Journal of Applied Genetics; 1998, 39, 4; 367-373
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber [Cucumis sativus L.]
Autorzy:: Plader, W
Hoshi, Y.
Malepszy, S.
Powiązania:: https://bibliotekanauki.pl/articles/2044260.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: heterochromatin
4',6-diamidino-2-phenylindole
euchromatin segment
hybridization
chromosome identification
mitotic chromosome
prometaphase chromosome
somatic cell
cucumber
DNA
chromomycin A3
Cucumis sativus
Opis:: Each of the seven chromosomes in cucumber (Cucumis sativus L.) was identified using sequential staining with Chromomycin A₃ (CMA) and 4-6-diamidino-2-phenylindole (DAPI) as DNA base-specific fluorescent dyes. The present method using enzymatic digestion produced a high level of well-spread early-metaphase chromosome complements. After CMA and DAPI staining, reproducible fluorescence bands were obtained in mitotic prometaphase chromosomes. The CMA staining method made it possible to characterize whole chromosomes from prometaphase to mid-metaphase. Chromosome 1 had the largest and widest CMA-positive (CMA⁺) band from the proximal region to the interstitial region on the long arm in prometaphase. A large gap separating of the short arm from the long arm was always observed in chromosome 2 during prometaphase. The banding pattern of the short arm was similar to that of the long arm in chromosome 2. Chromosomes 1 and 2 in early metaphase had sharp and large CMA-positive and DAPI-negative (CMA⁺DAPI⁻) bands at the pericentromeric regions. In early metaphase, chromosome 3 was characterized by having a narrow CMA⁺DAPI⁻ band on the pericentromeric region of the short arm. Chromosomes 4 and 5 showed similar chromosome length and had a large CMA⁺ band at the distal region of the long arm. Chromosome 4 did not show any clear band in the short arm, while chromosome 5 showed a telomeric CMA⁺ band at the short arm and a clear CMA⁺DAPI⁻ band at the pericentromeric region. Chromosome 6 had a CMA⁺ band at the distal region and a weak CMA⁺ band at the proximal region in each of the arms. Chromosome 7 had an evident CMA⁺ band in the long arm and a CMA⁺ DAPI⁻ band in the pericentromeric region.
Źródło:: Journal of Applied Genetics; 1998, 39, 3; 249-258
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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