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    Wyświetlanie 1-7 z 7
    Tytuł:
    Artificial extracellular matrices of collagen fibrils and lactoferrin as coatings to enhance osteoblast behavior
    Autorzy:
    Vandorovcova, M.
    Bacakova, L.
    Dubruel, P.
    Douglas, T. E. L.
    Powiązania:
    https://bibliotekanauki.pl/articles/284321.pdf
    Data publikacji:
    2012
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    coating
    collagen
    osteoblasts
    proliferation
    extracellular matrix
    lactoferrin
    Opis:
    Lactoferrin, a glycoprotein found in milk, has stimulated osteoblast proliferation and differentiation, but has remained relatively unexplored as a biomaterial component. In this study, artificial extracellular matrices consisting of fibrils of collagen type I containing lactoferrin were used as coatings for the biocompatible polymer poly(lactic-co-glycolic acid) (PLGA). The numbers of cells, their viability and proliferation rate were evaluated in various time intervals. Additionally, cell initial spreading area on day 1 was measured. The results show that lactoferrin accelerates fibril-logenesis, leads to increased osteoblast cell numbers 1 and 3 days post-seeding, and encourages their proliferation in each of the tested time intervals.
    Źródło:
    Engineering of Biomaterials; 2012, 15, no. 116-117 spec. iss.; 132-134
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Proliferacja osteoblastów Saos-2 oraz komórek endotelialnych EA.hy 926 na polerowanych i piaskowanych powierzchniach stopów tytanu Ti6Al4V i Ti6Al7Nb
    Proliferation of human osteoblast-like cells Saos-2 and human endothelial cells EA.hy 929 on polished and sandblasted surfaces of titanium alloys Ti6Al4V and Ti6Al7Nb
    Autorzy:
    Walkowiak-Przybyło, M.
    Walczyńska, M.
    Komorowski, P.
    Klimek, L.
    Walkowiak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/285427.pdf
    Data publikacji:
    2011
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    osteoblasty
    stopy tytanu
    proliferacja
    osteoblasts
    titanium alloys
    proliferation
    Źródło:
    Engineering of Biomaterials; 2011, 14, no. 109-111 spec. iss.; 39-41
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chemical modification of poly ε-caprolactone with wollastonite and its influence on biological properties of osteoblast like-cells MG-63
    Autorzy:
    Ścisłowska-Czarnecka, A.
    Menaszek, E.
    Kołaczkowska, E.
    Błażewicz, M.
    Podporska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/284898.pdf
    Data publikacji:
    2011
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    polycaprolactone
    wollastonite
    osteoblast-like MG-63 cells
    cell adhesion
    viability
    proliferation
    alkaline phosphatase
    Opis:
    PCL (poly-ε-caprolactone) is a biocompatible and biodegradable polymer of aliphatic polyester group. However, PCL does not effectively bind to the bone in contrast to bioactive inorganic compounds such as wollastonite. For this wollastonite (WS) is regarded as a potential bioactive material for bone tissue engeenering although its main drawback is brittlennes. Therefore we synthesized polymer nanocomposite materials composed of poly-ε-caprolactone and wollastonite (PCL/wollastonite) containing either 0.5% or 5% of the latter modifying filler. And we aimed to verify biological properties of the nanocomposite PCL/WS materials, in comparison to the pure PCL, on cultures of osteoblast-like cells MG-63. The study revealed that the adherence of the osteoblast-like cells to the tested materials was enhanced by the PCL modification (PCL/5WS > PCL/0.5WS > PCL) while cell viability/proliferation was not altered. Furthermore, the activity of alkaline phosphatase indicative of osteoblast differentiation (maturation) was enhanced when the cells were cultured with either PCL/5WS or PCL/0.5WS. Overall, our results indicate that PCL-modified wollastonite improves biological properties of the basic biomaterial suggesting its potential usefulness/application for the bone tissue regeneration.
    Źródło:
    Engineering of Biomaterials; 2011, 14, 102; 11-14
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films
    Autorzy:
    Parizek, M.
    Base, T.
    Londesborough, M. G. S.
    Lisa, V.
    Bacakova, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/285409.pdf
    Data publikacji:
    2008
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    metal coating
    gold film
    carboranethiol
    cell adhesion
    cell spreading
    cell proliferation
    biomaterials
    tissue engineering
    surgical implants
    Opis:
    Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2B10H11 (A), 1,2-(HS)2-1,2-C2B10H10 (B), 9,12-(HS)2-1,2-C2B10H10 (C) and 1,12-(HS)2-1,12- C2B10H10 (D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.
    Źródło:
    Engineering of Biomaterials; 2008, 11, no. 81-84; 117-119
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization
    Autorzy:
    Parizek, M.
    Kasalkova, N.
    Bacakova, L.
    Kolarova, K.
    Lisa, V.
    Svorcik, V.
    Powiązania:
    https://bibliotekanauki.pl/articles/285087.pdf
    Data publikacji:
    2009
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    Ar plasma discharge
    biomaterials
    low-density polyethylene
    cell adhesion
    cell proliferation
    grafting
    tissue engineering
    vascular smooth muscle cells
    Opis:
    Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.
    Źródło:
    Engineering of Biomaterials; 2009, 12, no. 89-91; 25-28
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Improved adhesion and growth of vascular smooth muscle cells in cultures on polyethylene modified by plasma discharge
    Autorzy:
    Parizek, M.
    Kasalkova, N.
    Bacakova, L.
    Kolarova, K.
    Lisa, V.
    Svorcik, V.
    Powiązania:
    https://bibliotekanauki.pl/articles/284295.pdf
    Data publikacji:
    2007
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    biomateriały
    inżynieria tkankowa
    Ar plasma discharge
    high density and low density polyethylene
    cell adhesion
    cell proliferation
    vascular smooth muscle cells
    biomaterials
    tissue engineering
    Opis:
    The attractiveness of synthetic polymers for cell colonization can be affected by physical and chemical modification of the polymer surface. In this study, high density polyethylene (HDPE, m.w. 0.952g/cm3) and low density polyethylene (LDPE, m.w. 0.922g/cm3) were modified by an Ar plasma discharge using Balzers SCD 050 device (exposure time 10, 50, 150 and 400 seconds, discharge power 1.7W). The material was then seeded with rat aortic smooth muscle cells (RASMC; passages 8 to 9, 17 000 cells/cm3) and incubated in a DMEM medium with 10% of fetal calf serum. On day 1 after seeding, the number of initially adhered cells was significantly higher on all modified HDPE and LDPE samples. On day 2, this difference persisted in HDPE, whereas in LDPE only the values on the samples modified by 150 and 400 seconds were significantly higher. On the 5th and 7th day, there were no significant differences in cell number among all LDPE samples. However, on the HDPE foils, significant differences were still apparent on the samples modified for 400 seconds. The cell spreading areas measured on day 1 after seeding were significantly larger on all modified LDPE samples, and, on day 2, on the HDPE samples exposed for 150s. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups in the polymer. These results suggest that the responsiveness of the cell to the changes in physiochemical surface properties was more pronounced in HDPE than in LDPE. On both types of polyethylene, the most appropriate exposure time for the enhancement of cell adhesion and growth seemed to be 150 and 400 seconds.
    Źródło:
    Engineering of Biomaterials; 2007, 10, no. 67-68; 1-4
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films
    Autorzy:
    Parizek, M.
    Base, T.
    Londesborough, M. G. S.
    Lisa, V.
    Bacakova, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/286249.pdf
    Data publikacji:
    2008
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    implanty chirurgiczne
    biomateriały
    powłoki metalowe
    inżynieria materiałowa
    metal coatings
    gold film
    carboranethiol
    cell adhesion
    cell spreading
    cell proliferation
    biomaterials
    tissue engineering
    surgical implants
    Opis:
    Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2 B10H11(A), 1,2-(HS)2-1,2-C2B10H10(B), 9,12-(HS)2-1,2-C2B10H10(C) and 1,12-(HS)2-1,12- C2B10H10(D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.
    Źródło:
    Engineering of Biomaterials; 2008, 11, 75; 6-8
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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