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    Wyświetlanie 1-10 z 10
    Tytuł:
    Conifer somatic embryogenesis: I. Development
    Autorzy:
    Klimaszewska, K
    Cyr, D.R.
    Powiązania:
    https://bibliotekanauki.pl/articles/41304.pdf
    Data publikacji:
    2002
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    somatic seedling
    initiation
    development
    somatic embryogenesis
    Pinaceae
    maturation
    embryogenesis
    conifer species
    forest industry
    forest
    somatic tree
    cryopreservation
    Opis:
    The discovery of conifer somatic embryogenesis (SE) and the subsequent development of SE protocols for a range of genera and species have opened new research opportunities to forest biotechnologists and a means towards mass clonal propagation for the forest industry. This paper provides a general description of the conifer SE process, followed by a review of protocols developed specifically for several conifer species in the Pinaceae family for which production of somatic trees has been demonstrated. Additionally, future research needs, including approaches for developing markers to optimize SE process and the production of high quality embryos, are discussed.
    Źródło:
    Dendrobiology; 2002, 48
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Conifer somatic embryogenesis: II. Applications
    Autorzy:
    Cyr, D R
    Klimaszewska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41451.pdf
    Data publikacji:
    2002
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    plantation forestry
    somatic seedling
    application
    somatic embryogenesis
    Pinaceae
    embryogenesis
    germplasm preservation
    clonal propagation
    Opis:
    Somatic embryogenesis (SE) of conifers for clonal propagation has emerged from its earliest beginnings in 1980 to become an integral component of tree improvement strategies. With its capacity for long-term germplasm preservation and scale-up technologies,it is seen to be the preferred avenue to accelerate selection and operational deployment of value-added genotypes. At present,programs for numerous species from the Pinaceae family are underway worldwide,with activities ranging from selection trials to pilot production for plantation forestry. This paper will provide a review of current efforts in conifer SE including cryopreservation,commercialization and deployment strategies,and transgenics. A discussion of challenges and issues is directed at genetic fidelity, intellectual property and future needs.
    Źródło:
    Dendrobiology; 2002, 48
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tree somatic embryogenesis in science and forestry
    Autorzy:
    Hazubska-Przybyl, T.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41255.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    tree
    somatic embryogenesis
    embryogenesis
    science
    forestry
    in vitro culture
    seedling
    somatic seedling
    forest management
    Opis:
    Somatic embryogenesis is the latest, and potentially the most efficient, method for the vegetative micropropagation of plants. Over the past three decades, numerous laboratory studies have investigated somatic embryogenesis of forest trees, yielding positive results for a number of economically important tree species. The first test trials were run and plantations were planted with interior spruce in the 90s by CellFor Inc. (Canada). However, at the beginning of the XXI century, the program to produce spruce and Douglas fir somatic seedlings was stopped for economic reasons. Thus, currently no operational program is ongoing except on a small scale in New Brunswick. In order to integrate somatic embryogenesis technology into operational reforestation programs, the production costs of forest tree somatic seedlings needs to be reduced, and the awareness of foresters and forest landowners that the material obtained through somatic embryogenesis is valuable needs to be increased. This awareness would enable implementation of this technology on a large scale for production and forest management throughout Europe including Poland. In this review, the importance of somatic embryogenesis in scientific research and in global and European forestry is presented. Our main aims are to provide basic information on the challenges in researching somatic embryogenesis of forest trees and to raise interest in this tree propagation technique in both scientists and foresters.
    Źródło:
    Dendrobiology; 2016, 76
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mutational witches’ broom impact on the growth of the parent branch in several Pinaceae species
    Autorzy:
    Zhuk, E.
    Vasilyeva, G.
    Goroshkevich, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2077653.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    somatic mutation
    annual rings
    crown part interaction
    Opis:
    Mutational witches’ broom is a part of the tree crown with abnormally dense branching and slow shoot growth, which putatively originates from a cell in a bud apical meristem and can be visually differentiated from the normal crown. The witches’ broom forms a large branching system which competes sufficiently with other parts of the tree crown. However, the competitive relationship and the interaction between the mutant and normal crown parts have not yet been studied. We investigated the patterns and dynamics of the competitive relationship between witches’ broom and normal branching systems of the same tree of five Pinaceae species by tree ring analysis. Three saw-cuts were made in each tree: on the lateral branch with the witches’ broom, on the proximal part of the axial branch in front of the branch with the witches’ broom, on the distal part of the axial branch right after the branch with the witches’ broom. An- nual radial growth was measured to the nearest 0.01 mm, and annual ring areas from three saw-cuts were analysed to compare the growth dynamics before and after witches’ broom appearance. The growth of the lateral branch with the witches’ broom sharply increased in the year of witches’ broom appearance by 2–10 times. The growth of the distal part of the axial branch sharply decreased in the same year, which could be explained only by the witches’ broom appearance. All branches with witches’ brooms gradually became thicker than the distal part of the branch, 3–150 times at the peak of growth, and occasionally outgrew even the proximal part of the branch. Thus, witches’ broom is drastically morphologically and physiologically different from the rest of the crown. All witches’ brooms had their own vertically oriented axis of symmetry and represented autonomous branching systems that were not subordinate to any other branching systems. They break the normal donor-acceptor relationship in a tree and eagerly consume resources without being fully involved in their production. The mutation clearly affects the hormonal regulation of growth and morphogenesis and turns a witches’ broom into a new metabolic sink.
    Źródło:
    Dendrobiology; 2020, 83; 52-59
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of culture medium formulation on Pinus nigra somatic embryogenesis
    Autorzy:
    Salaj, T.
    Fraterova, L.
    Carach, M.
    Salaj, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/41577.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    culture medium
    formulation
    Pinus nigra
    European black pine
    histology
    conifer
    plant growth regulator
    somatic embryo
    somatic embryogenesis
    Opis:
    The potential of immature zygotic embryos to produce embryogenic tissue was tested using culture media differed in inorganic salt composition as well as plant growth regulator content. Explants cultured on DCR as well as MLV media gave the highest initiation percentages with maximum values of 8.8% (MLV) to 10.4% (DCR). Plant growth regulators content (standard or reduced) had no profound effect on embryogenic tissue initiation. Somatic embryo maturation as well as germination was dependent on the cell line and salt composition of the medium. Plantlet regeneration occurred in three cell lines out of five tested. During the maturation process profound changes occurred in the internal organisation of somatic embryos such differentiation of root meristem in precotyledonary somatic embryos and formation of provascular strands in cotyledonary somatic embryos.
    Źródło:
    Dendrobiology; 2014, 71
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Induction of somatic embryogenesis in spruce: Picea omorika, P. pungens "Glauca", P. breweriana and P. abies
    Autorzy:
    Hazubska, T
    Szczygiel, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41201.pdf
    Data publikacji:
    2003
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Norway spruce
    Picea pungens
    Serbian spruce
    Picea abies
    blue spruce
    Picea omorika
    clonal propagation
    spruce
    somatic embryogenesis
    maturation
    embryogenesis
    Picea breweriana
    somatic embryo
    Opis:
    The studies were carried out on seed explants (mature zygotic embryos) of four spruce species: Picea omorika (Pancić) Purk., P. pungens ‘Glauca' Beisnn., P. breweriana S. Watson and P. abies (L.) Karst. The explants required darkness during the induction of embryogenic tissue. Temporary exposure of explants to light did not increase their embryogenic capacity. It was observed that the temperature of 25°C was the optimal for embryogenic tissue induction from mature zygotic embryos of Picea omorika. Cold treatment at 4°C during one week improved embryogenic tissue initiation in the explants of P. pungens ‘Glauca'. Seed explants of Picea omorika produced embryogenic tissue on medium lacking glutamine. Only explants of Picea abies and P. omorika showed embryogenic tissue formation. For effective proliferation of embryogenic tissue in Picea abies and P. omorika it was necessary to supply the medium with 2,4-D (4.5-9 μM) and BA (2.25 μM). Two of three analized lines showed that ABA promote somatic embryo maturation, at the ABA concentration 20 μM in the medium.
    Źródło:
    Dendrobiology; 2003, 50
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stimulation of somatic embryo growth and development in Picea spp. by polyethylene glycol
    Autorzy:
    Hazubska-Przybył, Teresa
    Wawrzyniak, Mikołaj
    Powiązania:
    https://bibliotekanauki.pl/articles/956841.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    somatic embryogenesis
    osmotic stress
    germination
    norway spruce
    serbian spruce
    Opis:
    The objective of the presented study was to compare the effect of osmotic stress, induced by various concentrations of polyethylene glycol (PEG) MW 4000, on the development, maturation, and conversion of somatic embryos of Picea abies (model species) and P. omorika (endemic species) into somatic seedlings. Embryogenic tissue (ET) of P. abies (line A and B) and P. omorika were incubated on a maturation medium with 20 μM abscisic acid (ABA), 1 μM indole-3-butyric acid (IBA) and PEG 4000 at 0, 5, and 7.5%. After 5 weeks, the number of somatic embryos produced, and the number of cotyledonary somatic embryos per 1 gram of ET, was assessed. Cotyledonary somatic embryos were selected and transferred to a Margara germination medium for two months. Their growth, as measured by hypocotyl and radicle length, was evaluated after one month, and the somatic embryos were transferred to fresh medium. After the second month of incubation, the conversion frequency of germinated embryos into the seedlings was assessed. In general, ETs of both of the tested spruce species cultured on a maturation medium amended with 5% PEG 4000 exhibited a tendency to produce a higher total number of embryos, as well as a higher number of cotyledonary embryos. The B line of P. abies produced a significantly greater number of cotyledonary embryos, relative to the control (no PEG). PEG only stimulated hypocotyl growth in embryos of both lines (A and B) of P. abies during the germination stage. Somatic embryos of Picea omorika were characterized by poor growth of both hypocotyls and radicles, irrespective of PEG treatment. The addition of PEG 4000 to the maturation medium did not improve the conversion frequency of germinated somatic embryos into somatic seedlings in either spruce species after a month of incubation on Margara medium. The effect of PEG 4000 on somatic embryogenesis efficiency was more evident in the ET of the two P. abies lines, relative to the P. omorika ET line, during both the maturation and the germination stage. Results indicated that the P. omorika ET line was almost completely insensitive to all of the PEG concentrations tested.
    Źródło:
    Dendrobiology; 2017, 78; 168-178
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study of Abies somatic embryogenesis and its application
    Autorzy:
    Vookova, B.
    Kormutak, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/41461.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Abies
    fir
    protein analysis
    chlorophyll content
    macroelement
    microelement
    incompatible crossing
    somatic embryogenesis
    application
    Opis:
    This paper provides results of somatic embryogenesis study in our laboratory. General description of somatic embryogenesis (SE) induction, maturation of somatic embryos and plantlets regeneration of the Abies species, followed by a comparisons of some characteristics of zygotic and somatic embryos, seedlings and emblings (somatic seedlings). Own results are supplemented with some literature data. Also aplication of SE for improving of plantlet regeneration of elite fir trees from Dobroč primeval is described as well as initiation of the SE from seeds of incompatible crossings of firs where zygotic embryos abort usually several weeks after pollination
    Źródło:
    Dendrobiology; 2014, 71
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Embryogenic callus induction and differentiation in silver fir (Abies alba Mill.) tissue cultures
    Autorzy:
    Nawrot-Chorabik, K
    Powiązania:
    https://bibliotekanauki.pl/articles/41643.pdf
    Data publikacji:
    2008
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    silver fir
    Abies alba
    tissue culture
    embryogenic suspensor mass
    somatic embryogenesis
    callus induction
    differentiation
    Opis:
    The research was conducted on explants of silver fir (Abies alba Mill.) deriving from several forest districts in southern Poland. The study encompassed the influence of the origin of plant material, type of explants, kind of substances used for explants sterilization, PPM and the type of medium on the ability to form embryogenic callus and to develop somatic embryos in silver fir explants. From the plant material collected in three sites, 57 clones were obtained from mature zygotic embryos; this produced an embryogenesis frequency of 6%. Embryogenic callus was obtained with a diameter of 65–70 mm depending on the material origin. The best medium for development of callus inducted on embryos isolated from mature silver fir seeds was the SH medium. Somatic embryos were formed in a globular stadium (24 pieces) on this medium. The 10% solution of NaOCl (used for 15 minutes) turned out to be the most effective substance for seed sterilization.
    Źródło:
    Dendrobiology; 2008, 59; 31-40
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Structure of embryogenic tissues and accumulation of storage materials in somatic embryos of Picea abies and P. omorika
    Autorzy:
    Hazubska-Przybyl, T
    Bojarczuk, K.
    Guzicka, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/41109.pdf
    Data publikacji:
    2008
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Picea abies
    Picea omorica
    tissue
    spruce
    development
    starch grain
    lipid body
    embryogenic tissue
    storage material
    somatic embryo
    plant tissue
    Opis:
    Microscopic analysis of embryogenic tissues of Picea abies and P. omorika showed that the tissues differed in proportions of various types of proembryogenic masses, and in presence/absence of early somatic embryos, which suggests that they differed in embryogenic potential. On the basis of this study, embryogenic competence of tissues can be assessed at early stages of somatic embryogenesis, and thus the most efficient lines can be selected (one Picea abies and two P. omorika). In the course of development of somatic embryos embryogenic tissue was placed on a medium with abscisic acid (ABA), which affects an increase in osmotic pressure at the stage of maturation of somatic embryos and finally on the regeneration of cotyledonary embryos. In this stage of embryos development, storage materials were accumulated, which are used in specific metabolic processes during embryo development.
    Źródło:
    Dendrobiology; 2008, 60; 19-28
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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