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    Wyświetlanie 1-10 z 10
    Tytuł:
    Utility of two mitochondrial markers for identification of Picea abies refugial origin
    Autorzy:
    Litkowiec, M
    Dering, M.
    Lewandowski, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/41333.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    coniferous plant
    tree
    Norway spruce
    Picea abies
    mtDNA
    molecular marker
    mitochondrial marker
    identification
    polymerase chain reaction
    RFLP analysis
    gene pool conservation
    forest ecosystem
    plant population
    Opis:
    Picea abies (L.) Karst is one of the most important coniferous species of Europe from both ecological and economical points of view. Traditional methods for the gene pool conservation and biodiversity maintenance in forest ecosystems have been practiced in many countries. For progress in this field using highly polymorphic genetic molecular markers is needed. Our goal was to demonstrate the utility of two polymorphic mitochondrial markers mt15-D02 and nad1 b/c in identification native Norway spruce stands. This molecular markers were tested in 1401 individuals from 59 Polish Norway spruce populations. We detected three alleles, which are called1, 2 and3, for locus mt15-D02 and two alleles , which are called1 and2, for locus nad1 b/c in our material. All five variants of alleles indicate the natural origin of P. abies. Result of this study shows that molecular marker mt15-D02 is easy to use and more informative in compare to marker nad1 b/c.
    Źródło:
    Dendrobiology; 2009, 61; 65-71
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identyfing alien genotypes and their consequences for genetic variationin clonal seed orchards of Pinus sylvestris L.
    Autorzy:
    Przybylski, P.
    Kowalczyk, J.
    Odrzykoski, I.
    Matras, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2077755.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    genetic analysis
    Pinus sylvestris
    tree breeding
    isoenzyme marker
    grafting error
    Opis:
    This study investigates the rates of grafting and planting errors that occur in seed orchards, and evaluates their effects on the genetic diversity and relatedness of genotypes. The study used three clonal Scots pine seed orchards of differing ages and clonal composition located in the forest districts of Susz, Pniewy and Zdrojowa Góra, Poland. Maximum breeding ability within a seed orchard requires isolated from external sources of pollen, and have no alien genotypes inside the orchard. We used 13 isoenzyme markers to determine the genotypic identity of ramets and compared the number of genotypes of the actual current ramet population (W1) with genotypes comprising the originally intended plus trees (designated as W0) to estimate the genotypic assignment error rate per orchard. For both W0 and W1, we calculated the effective number of clones and the relative effective number of clones. Ramet assignment errors were detected in all three seed orchards. Gnotypic errors ranged from 5.8% to 37.7% across orchards. A total of 46 alleles were found, with the mean number of alleles per locus ranging from 2.77 to 3.23. At individual loci, the level of observed heterozygosity was variable. Alien genotypes had negligible effects on seed orchard heterozygosity. The Fst values between seed orchards amounted to 0.6% between Susz and Pniewy and 1.1% between Susz and Zdrojowa Góra. The effect on genetic variation of ramet assignment errors was small and influenced genetic diversity only in the case of the Susz seed orchard. However, our results suggest that negative effects of alien genotypes can occur on breeding value of seeds from analysed seeds orchards.
    Źródło:
    Dendrobiology; 2019, 81; 40-46
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Distribution, occurrence, and cluster analysis of new polyprenyl acetones and other polyisoprenoids from North Sumatran mangroves
    Autorzy:
    Basyuni, Mohammad
    Sagami, Hiroshi
    Baba, Shigeyuki
    Oku, Hirosuke
    Powiązania:
    https://bibliotekanauki.pl/articles/956820.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    chemotaxonomic marker
    dolichol
    indonesian mangroves
    polyprenol
    two-dimensional thin
    layer chromatography
    Opis:
    Background. Mangrove forests have long been known as a source of phytochemical compounds producing various secondary metabolites. Despite the ubiquitous diversity of polyisoprenoids in the plant kingdom, few studies have focused on the distribution of polyisoprenoids in mangrove plants. The present study describes the distribution and occurrence of a new class of prenyl derivates – polyprenyl acetone as well as other polyisoprenoids in fourteen species of Indonesian mangroves, with an emphasis on chemotaxonomic importance. Material and methods. The leaves and roots of fourteen North Sumatran mangroves were analyzed using two-dimensional thin layer chromatography and electrospray ionization mass spectrometry. Results. In the leaves, the distribution of several types of polyprenyl acetones, polyprenols, and dolichols was detected and classified into types: type-I, having a predominance of dolichols over polyprenols (more than nine-fold), was observed in Acrostichum aureum (younger leaves), Avicennia alba, Av. lanata, Av. officinalis, Bruguiera parviflora, Ceriops tagal, Nypa fruticans, and Rhizophora mucronata; type-II, having the presence of both polyprenols and dolichols, was observed in Acanthus ilicifolius, Acr. aureum, B. cylindrica, and R. apiculata; type-III having a predominance of polyprenols over dolichols (more than nine-fold), was not observed in any North Sumatran mangroves; type-IV, having the presence of both polyprenyl acetones and dolichols, was observed in Aegiceras corniculatum; type-V, having the presence of polyprenyl acetones, polyprenols, and dolichols, was observed in Sonneratia caseolaris and Xylocarpus granatum. In the roots, type-I distribution was observed in Ae. corniculatum, Av. alba, Av. lanata, Av. officinalis, B. parviflora, C. tagal, N. fruticans, R. apiculata, R. mucronata, S. caseolaris, and X. granatum. Type-II distribution was observed in Ac. ilicifolius, Acr. aureum, and B. cylindrica. Type-III, -IV, and -V distributions were not observed in mangrove roots. Cluster analysis demonstrated that polyisoprenoid patterns in the leaves and roots form distinct separation into appropriate genera and tribe, suggesting that mangrove polyisoprenoids are chemotaxonomically significant. Conclusions. The major polyisoprenoid alcohols in Indonesian mangroves were found to be dolichols rather than polyprenols. The diversity of polyisoprenoids in both leaves and roots of mangroves may provide chemotaxonomic marker. The discovery of a new class of polyprenyl acetone is the first report from mangrove plants.
    Źródło:
    Dendrobiology; 2017, 78; 18-31
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic variability of Pinus sylvestris populations from IUFRO 1982 provenance trial
    Autorzy:
    Androsiuk, P.
    Urbaniak, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/40971.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    genetic variability
    Scotch pine
    Pinus sylvestris
    plant population
    molecular marker
    provenance trial
    Opis:
    Provenance trials were designed to analyse the quantitative responses of tree species to environmental variables found in different experiment location. However, we have still limited knowledge how natural and artificial selection affects genetic variation of the species populations gather in such experimental sites. We have used bulked DNA-based RAPD and ISJ analysis to investigate genetic diversity and differentiation of Scots pine populations from two Polish locations of IUFRO 1982 provenance trial placed in Kórnik and in Supraśl. Applied categories of DNA markers differed in terms of revealing genetic diversity of the species. Ten RAPD primers applied in the study yielded a total of 75 bands, of which 21 (28%) and 15 (20%) were polymorphic in Kórnik and in Supraśl, respectively. Six ISJ primers revealed 42 bands of which 4 (9.52%) and 14 (33.3%) were polymorphic in Kórnik and in Supraśl, respectively. The genetic diversity and differentiation was low, as expressed by He=0.071 and He=0.085, and by genetic distance values which ranged from 0.0 to 0.240 (on average 0.081) and from 0.017 to 0.188 (on average 0.094) for Kórnik and Supraśl, respectively. Location of provenance trial appeared to have a significant influence on revealed level of genetic polymorphism and pattern of interpopulation differentiation. However, genetic structure found for analysed Scots pine provenances from IUFRO 1982 in Kórnik was also confirmed for Supraśl experimental site. In the light of available data we also discussed the influence of historical migration routes and gene flow on observed genetic variation of the species.
    Źródło:
    Dendrobiology; 2014, 71
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity of North African Thymus algeriensis in Tunisia: Population structure and implication for conservation
    Autorzy:
    Ben El Hadj Ali, I.
    Guetat, A.
    Boussaid, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/41188.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    genetic diversity
    North Africa
    Thymus algeriensis
    Tunisia
    population structure
    molecular marker
    natural population
    conservation
    Opis:
    The genetic diversity within and among nine natural populations of Thymus hirtus Willd. subsp. algeriensis (Boiss. et Reut.) Murb. from different geographical and bioclimatic zones were assessed using Random Amplified Polymorphic DNA data. A total of 154 bands were generated from seven selected primers. 141 bands were polymorphic (P=91.56%). The genetic diversity within a population based on Shannon's index which was high (H'pop=0.307) and varied according to bioclimatic zones. A high genetic differentiation among populations (GST=0.335 and FST=0.296) was revealed, suggesting a population isolation and a low level of gene flow among them. The major proportion of the variation was attributable to individual differences within populations. The genetic structure is in accordance with geography distances. The Neighbour- joining tree based on Nei's and Li's genetic distance among individuals showed that individuals from each population clustered together. The UPGMA dendrogram based on FST values revealed three clusters each of them includes populations closuring to their geographical origin. The high genetic structure of populations resulted from genetic drift caused both by the habitat fragmentation and the low size of most populations. The in situ conservation should interest all populations. The ex situ conservation should be based on the collection of seeds rather within than among populations because of the maximum of variation was revealed within populations.
    Źródło:
    Dendrobiology; 2012, 67
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity of wild Thymus capitatus (Lamiaceae) in Tunisia using molecular markers
    Autorzy:
    Ben El Hadj Ali, I.
    Guetat, A.
    Boussaid, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/41663.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    genetic diversity
    wild plant
    Thymus capitatus
    Lamiaceae
    Tunisia
    molecular marker
    habitat fragmentation
    conservation strategy
    genetic structure
    Opis:
    In Tunisia, Thymus capitatus L. populations are severely destroyed due to deforestation and over-collecting. The species occurs in small scattered populations decreasing progressively in size. Yet, no conservation or improvement programs are attempted to preserve and promote the potential value of this resource. In this work, we assessed the genetic diversity of nine Tunisian populations of Thymus capitatus L. from different bioclimates, using 103 polymorphic randomly amplified polymorphic DNA (RAPD) loci. The analysis of the genetic variation within and among populations is primordial to elaborate conservation and improvement programs. The species showed a low diversity within populations (0.276
    Źródło:
    Dendrobiology; 2012, 68
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo
    Autorzy:
    Wachowiak, W
    Baczkiewicz, A.
    Celinski, K.
    Prus-Glowacki, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/41357.pdf
    Data publikacji:
    2004
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Scotch pine
    Pinus sylvestris
    Pinus mugo
    dwarf pine
    hybridization
    DNA marker
    mtDNA
    trnV-rbcL region
    chloroplast
    DNA polymorphism
    Opis:
    Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.
    Źródło:
    Dendrobiology; 2004, 51; 67-72
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic variation of Picea abies in southern Germany as determined using isozyme and STS markers
    Autorzy:
    Konnert, M
    Powiązania:
    https://bibliotekanauki.pl/articles/40969.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    international conference
    Europe
    forest ecosystem
    plant breeding
    tree
    Norway spruce
    Picea abies
    genetic variation
    Germany
    isoenzyme
    Bavaria
    DNA marker
    plant genetics
    provenance
    Opis:
    Over 50 populations of Norway spruce from Bavaria were analysed at 23 isozyme gene loci. The mean genetic distances between these populations were quite small. A geographical grouping could not be observed, and discrimination between provenances from high and low altitudes was not identifiable using this marker type, either. The only difference between spruce populations from South Bavaria and those from Northeast Bavaria is in the presence of some distinct rare alleles. The highest values for the genetic diversity were detected for spruce stands in Northeast Bavaria (Frankonian Forest). Using STS markers, further genes of the nuclear genome of Picea abies can be dealt with. The genetic differences found on the basis of ten STS markers between different Picea abies seed lots and/or seedling populations are generally 2-3 times greater than those found by means of isozyme gene markers. DNA markers turned out to be an appropriate and substantial addition or even more a suitable alternative to isozyme markers for analysing genetic variation and testing provenance identity. Their advantages consist in a markedly wider variation as well as in the enlarged genome segments investigated.
    Źródło:
    Dendrobiology; 2009, 61 Supplement
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mitochondrial and nuclear DNA differentiation of Picea abies populations in Poland
    Autorzy:
    Nowakowska, J A
    Powiązania:
    https://bibliotekanauki.pl/articles/40976.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    international conference
    Europe
    forest ecosystem
    plant breeding
    tree
    Norway spruce
    Picea abies
    Polska
    mitochondrial DNA
    nuclear DNA
    plant population
    microsatellite marker
    post-glacial migration
    Opis:
    The natural stands of Norway spruce in Poland are split between the southern and the northeastern parts of the country. Two so-called "spruceless" zones separate the northern spruce locations from those in the south, one "spruceless" zone is situated in Central Poland, and the other one in the Beskid Mts. Mitochondrial (STS) and nuclear (SSR) markers were used to perform the genetic identification of Norway spruce. Four different variants of haplotypes, "a", "b", "c" and "d", were found to occur in the nad1 locus of STS markers. Populations from the northern range of Picea abies distribution in Poland harboured exclusively haplotypes "c" and "d", except for the Białowieża population which had haplotypes "a" and "c". Populations from the "spruceless" zones contained four types of haplotypes whilst those from southern Poland were mostly composed of haplotype "a". High mean gene diversity was observed for both STS and SSR markers (HT = 0.529, and HT = 0.851, respectively). The total genetic differentiation of Norway spruce populations was very low (FST= 0.088). Two main groups of populations were distinguished in the dendrogram defined by Nei's genetic distances based on microsatellite markers. The distribution of the genotypes was scattered and did not show any connection with the spatial distribution of P. abies in Poland. Only the mtDNA markers were able to differentiate the northern populations of Norway spruce from the southern ones, proving the historical separation between the Baltico-Nordic and the Hercyno-Carpathian ranges of P. abies in Poland. By contrast, the microsatellite data suggested an overlap between the genotypes due to the human manipulation of Norway spruce stands in the past.
    Źródło:
    Dendrobiology; 2009, 61 Supplement
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic structure of Picea abies populations growing on extreme sites as revealed by isoenzyme markers: a case study from Slovenia and Bosnia and Herzegovina
    Autorzy:
    Ballian, D
    Bogunic, F.
    Bozic, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/41007.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    international conference
    Europe
    forest ecosystem
    plant breeding
    tree
    Norway spruce
    Picea abies
    plant population
    genetic structure
    extreme site
    isoenzyme marker
    genetic variation
    gene polymorphism
    forest tree
    Slovenia
    Bosnia and Herzegovina
    forest community
    Sphagno-Piceetum community
    Opis:
    Three populations of Norway spruce from ecologically extreme environments in Slovenia and Bosnia and Herzegovina were examined for genetic polymorphism. The spruces there grow in specific forest communities (Sphagno-Piceetum) which represent the remnants of the post-glacial vegetation. The aim of the study was to search for similarities in the genetic variation among populations adapted to such conditions. In total, 10 isoenzyme systems involving 16 gene loci were analysed. The results showed differences in genetic differentiation at loci Got-B, Skdh-A and 6-Pgdh-C between the two Slovenian populations and the Bosnian population, but also indicated an interestingly close relationship between the Slovenian population Pohorje and the Bosnian population Nišići.
    Źródło:
    Dendrobiology; 2009, 61 Supplement
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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