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Wyszukujesz frazę "embryogenesis" wg kryterium: Temat


Wyświetlanie 1-8 z 8
Tytuł:
Conifer somatic embryogenesis: II. Applications
Autorzy:
Cyr, D R
Klimaszewska, K.
Powiązania:
https://bibliotekanauki.pl/articles/41451.pdf
Data publikacji:
2002
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
plantation forestry
somatic seedling
application
somatic embryogenesis
Pinaceae
embryogenesis
germplasm preservation
clonal propagation
Opis:
Somatic embryogenesis (SE) of conifers for clonal propagation has emerged from its earliest beginnings in 1980 to become an integral component of tree improvement strategies. With its capacity for long-term germplasm preservation and scale-up technologies,it is seen to be the preferred avenue to accelerate selection and operational deployment of value-added genotypes. At present,programs for numerous species from the Pinaceae family are underway worldwide,with activities ranging from selection trials to pilot production for plantation forestry. This paper will provide a review of current efforts in conifer SE including cryopreservation,commercialization and deployment strategies,and transgenics. A discussion of challenges and issues is directed at genetic fidelity, intellectual property and future needs.
Źródło:
Dendrobiology; 2002, 48
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tree somatic embryogenesis in science and forestry
Autorzy:
Hazubska-Przybyl, T.
Bojarczuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/41255.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
tree
somatic embryogenesis
embryogenesis
science
forestry
in vitro culture
seedling
somatic seedling
forest management
Opis:
Somatic embryogenesis is the latest, and potentially the most efficient, method for the vegetative micropropagation of plants. Over the past three decades, numerous laboratory studies have investigated somatic embryogenesis of forest trees, yielding positive results for a number of economically important tree species. The first test trials were run and plantations were planted with interior spruce in the 90s by CellFor Inc. (Canada). However, at the beginning of the XXI century, the program to produce spruce and Douglas fir somatic seedlings was stopped for economic reasons. Thus, currently no operational program is ongoing except on a small scale in New Brunswick. In order to integrate somatic embryogenesis technology into operational reforestation programs, the production costs of forest tree somatic seedlings needs to be reduced, and the awareness of foresters and forest landowners that the material obtained through somatic embryogenesis is valuable needs to be increased. This awareness would enable implementation of this technology on a large scale for production and forest management throughout Europe including Poland. In this review, the importance of somatic embryogenesis in scientific research and in global and European forestry is presented. Our main aims are to provide basic information on the challenges in researching somatic embryogenesis of forest trees and to raise interest in this tree propagation technique in both scientists and foresters.
Źródło:
Dendrobiology; 2016, 76
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Conifer somatic embryogenesis: I. Development
Autorzy:
Klimaszewska, K
Cyr, D.R.
Powiązania:
https://bibliotekanauki.pl/articles/41304.pdf
Data publikacji:
2002
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
somatic seedling
initiation
development
somatic embryogenesis
Pinaceae
maturation
embryogenesis
conifer species
forest industry
forest
somatic tree
cryopreservation
Opis:
The discovery of conifer somatic embryogenesis (SE) and the subsequent development of SE protocols for a range of genera and species have opened new research opportunities to forest biotechnologists and a means towards mass clonal propagation for the forest industry. This paper provides a general description of the conifer SE process, followed by a review of protocols developed specifically for several conifer species in the Pinaceae family for which production of somatic trees has been demonstrated. Additionally, future research needs, including approaches for developing markers to optimize SE process and the production of high quality embryos, are discussed.
Źródło:
Dendrobiology; 2002, 48
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induction of somatic embryogenesis in spruce: Picea omorika, P. pungens "Glauca", P. breweriana and P. abies
Autorzy:
Hazubska, T
Szczygiel, K.
Powiązania:
https://bibliotekanauki.pl/articles/41201.pdf
Data publikacji:
2003
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Norway spruce
Picea pungens
Serbian spruce
Picea abies
blue spruce
Picea omorika
clonal propagation
spruce
somatic embryogenesis
maturation
embryogenesis
Picea breweriana
somatic embryo
Opis:
The studies were carried out on seed explants (mature zygotic embryos) of four spruce species: Picea omorika (Pancić) Purk., P. pungens ‘Glauca' Beisnn., P. breweriana S. Watson and P. abies (L.) Karst. The explants required darkness during the induction of embryogenic tissue. Temporary exposure of explants to light did not increase their embryogenic capacity. It was observed that the temperature of 25°C was the optimal for embryogenic tissue induction from mature zygotic embryos of Picea omorika. Cold treatment at 4°C during one week improved embryogenic tissue initiation in the explants of P. pungens ‘Glauca'. Seed explants of Picea omorika produced embryogenic tissue on medium lacking glutamine. Only explants of Picea abies and P. omorika showed embryogenic tissue formation. For effective proliferation of embryogenic tissue in Picea abies and P. omorika it was necessary to supply the medium with 2,4-D (4.5-9 μM) and BA (2.25 μM). Two of three analized lines showed that ABA promote somatic embryo maturation, at the ABA concentration 20 μM in the medium.
Źródło:
Dendrobiology; 2003, 50
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stimulation of somatic embryo growth and development in Picea spp. by polyethylene glycol
Autorzy:
Hazubska-Przybył, Teresa
Wawrzyniak, Mikołaj
Powiązania:
https://bibliotekanauki.pl/articles/956841.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
somatic embryogenesis
osmotic stress
germination
norway spruce
serbian spruce
Opis:
The objective of the presented study was to compare the effect of osmotic stress, induced by various concentrations of polyethylene glycol (PEG) MW 4000, on the development, maturation, and conversion of somatic embryos of Picea abies (model species) and P. omorika (endemic species) into somatic seedlings. Embryogenic tissue (ET) of P. abies (line A and B) and P. omorika were incubated on a maturation medium with 20 μM abscisic acid (ABA), 1 μM indole-3-butyric acid (IBA) and PEG 4000 at 0, 5, and 7.5%. After 5 weeks, the number of somatic embryos produced, and the number of cotyledonary somatic embryos per 1 gram of ET, was assessed. Cotyledonary somatic embryos were selected and transferred to a Margara germination medium for two months. Their growth, as measured by hypocotyl and radicle length, was evaluated after one month, and the somatic embryos were transferred to fresh medium. After the second month of incubation, the conversion frequency of germinated embryos into the seedlings was assessed. In general, ETs of both of the tested spruce species cultured on a maturation medium amended with 5% PEG 4000 exhibited a tendency to produce a higher total number of embryos, as well as a higher number of cotyledonary embryos. The B line of P. abies produced a significantly greater number of cotyledonary embryos, relative to the control (no PEG). PEG only stimulated hypocotyl growth in embryos of both lines (A and B) of P. abies during the germination stage. Somatic embryos of Picea omorika were characterized by poor growth of both hypocotyls and radicles, irrespective of PEG treatment. The addition of PEG 4000 to the maturation medium did not improve the conversion frequency of germinated somatic embryos into somatic seedlings in either spruce species after a month of incubation on Margara medium. The effect of PEG 4000 on somatic embryogenesis efficiency was more evident in the ET of the two P. abies lines, relative to the P. omorika ET line, during both the maturation and the germination stage. Results indicated that the P. omorika ET line was almost completely insensitive to all of the PEG concentrations tested.
Źródło:
Dendrobiology; 2017, 78; 168-178
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study of Abies somatic embryogenesis and its application
Autorzy:
Vookova, B.
Kormutak, A.
Powiązania:
https://bibliotekanauki.pl/articles/41461.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Abies
fir
protein analysis
chlorophyll content
macroelement
microelement
incompatible crossing
somatic embryogenesis
application
Opis:
This paper provides results of somatic embryogenesis study in our laboratory. General description of somatic embryogenesis (SE) induction, maturation of somatic embryos and plantlets regeneration of the Abies species, followed by a comparisons of some characteristics of zygotic and somatic embryos, seedlings and emblings (somatic seedlings). Own results are supplemented with some literature data. Also aplication of SE for improving of plantlet regeneration of elite fir trees from Dobroč primeval is described as well as initiation of the SE from seeds of incompatible crossings of firs where zygotic embryos abort usually several weeks after pollination
Źródło:
Dendrobiology; 2014, 71
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Embryogenic callus induction and differentiation in silver fir (Abies alba Mill.) tissue cultures
Autorzy:
Nawrot-Chorabik, K
Powiązania:
https://bibliotekanauki.pl/articles/41643.pdf
Data publikacji:
2008
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
silver fir
Abies alba
tissue culture
embryogenic suspensor mass
somatic embryogenesis
callus induction
differentiation
Opis:
The research was conducted on explants of silver fir (Abies alba Mill.) deriving from several forest districts in southern Poland. The study encompassed the influence of the origin of plant material, type of explants, kind of substances used for explants sterilization, PPM and the type of medium on the ability to form embryogenic callus and to develop somatic embryos in silver fir explants. From the plant material collected in three sites, 57 clones were obtained from mature zygotic embryos; this produced an embryogenesis frequency of 6%. Embryogenic callus was obtained with a diameter of 65–70 mm depending on the material origin. The best medium for development of callus inducted on embryos isolated from mature silver fir seeds was the SH medium. Somatic embryos were formed in a globular stadium (24 pieces) on this medium. The 10% solution of NaOCl (used for 15 minutes) turned out to be the most effective substance for seed sterilization.
Źródło:
Dendrobiology; 2008, 59; 31-40
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of culture medium formulation on Pinus nigra somatic embryogenesis
Autorzy:
Salaj, T.
Fraterova, L.
Carach, M.
Salaj, J.
Powiązania:
https://bibliotekanauki.pl/articles/41577.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
culture medium
formulation
Pinus nigra
European black pine
histology
conifer
plant growth regulator
somatic embryo
somatic embryogenesis
Opis:
The potential of immature zygotic embryos to produce embryogenic tissue was tested using culture media differed in inorganic salt composition as well as plant growth regulator content. Explants cultured on DCR as well as MLV media gave the highest initiation percentages with maximum values of 8.8% (MLV) to 10.4% (DCR). Plant growth regulators content (standard or reduced) had no profound effect on embryogenic tissue initiation. Somatic embryo maturation as well as germination was dependent on the cell line and salt composition of the medium. Plantlet regeneration occurred in three cell lines out of five tested. During the maturation process profound changes occurred in the internal organisation of somatic embryos such differentiation of root meristem in precotyledonary somatic embryos and formation of provascular strands in cotyledonary somatic embryos.
Źródło:
Dendrobiology; 2014, 71
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-8 z 8

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