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Tytuł:
Gene pool conservation programme of Norway spruce Picea abies (L.) Karst. in the Beskid Mts
Autorzy:
Jurasek, M
Powiązania:
https://bibliotekanauki.pl/articles/41497.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Czech Republic
Czech State Forests
Norway spruce
Picea abies
conservation
reproduction
seed orchard
clone
artificial regeneration
seed stand
gene pool conservation
conservation programme
seed bank
Opis:
The paper describes the measures adopted by the Czech State Forests for the conservation and reproduction of the Norway spruce gene pool in the Moravian-Silesian Beskid Mts. In addition to the assurance of natural regeneration in genetically valuable stands and the use of high-quality autochthonous stands as seed sources for artificial regeneration, the measures include selection of plus-trees, establishment of seed orchards, seed stands and clone archives, and identification of gene pools. Seeds of the most valuable regional populations are deposited in a seed bank.
Źródło:
Dendrobiology; 2004, 51 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trunks of multi-stem black poplars may have different genotypes – evidence from the Oder valley in Poland
Autorzy:
Zukowska, W.B.
Wojkiewicz, B.
Lewandowski, A.
Powiązania:
https://bibliotekanauki.pl/articles/2078277.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Populus nigra
genets
ramets
conservation programs
microsatellites
Opis:
The black poplar (Populus nigra L.) is an ecologically and economically important tree species, characteristic for floodplain forests in Europe. It is now considered to be endangered in many European countries due to the loss of its natural habitat caused by the development of river engineering and intensive land use management of riverside areas. Multi-stem specimens can be found occasionally in natural black poplar populations. The percentage of such individuals is low, although in some stands it may be over a doz- en percent. The origin of multi-stem trees may be different. As a consequence, trunks being part of such a tree can have the same or different genotypes. The study aimed to find out whether all trunks of multi-stem black poplars are ramets of the same genet or if their genotypes are different, in which case they increase the gene pool of the population. We used 13 microsatellite nuclear markers to analyze the genotypes of 83 trunks belonging to 34 multi-stem black poplar trees from five natural populations from the Oder river valley in Poland. We also performed the sibship analysis of all trunks. The results showed that the trunks being part of the particular individual had distinct genotypes in five trees (14.7%). This indicates that they were formed as a result of the fusion of two genetically different individuals. The sibship analysis revealed that one multi-stem tree was represented by full-sibs. In the remaining four trees the fused trunks were not related. Nevertheless, we found three pairs of half-sibs between different trees that were distant from each other. The majority of the multi-stem trees we examined developed most probably due to the damage to the apical meristem of the main shoot of a single-stem tree. Nevertheless, some of them were indeed formed from two distinct individuals. Therefore, if a particular population consists of a small number of individuals, we recommend to study the genotypic homogeneity of each multi-stem tree.
Źródło:
Dendrobiology; 2021, 86; 1-7
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diversity, distribution and threats of the Juglandaceae in Vietnam
Autorzy:
Van Sam, H.
Quang Tung, D.
Jasinska, A.K.
Rion, F.
Tuyen, P.T.
Ngoc, D.T.B.
Tam, D.T.
Bétrisey, S.
Song, Y.G.
Kozlowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/2078269.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Conservation biology
relict trees
Rhoiptelea
Engelhardia
Alfaropsis
Opis:
Vietnam is one of the main centers of generic diversity for Juglandaceae worldwide. In this study, we present for the first time a province-wide distribution of all 3 subfamilies, 7 genera, and 11 Vietnamese species, and give an update on the habitats of all Vietnamese Juglandaceae species, their uses, and current threats. Juglandaceae are found throughout Vietnam. However, most species occur predominantly in the north- ern part of the country between 600 and 1200 m. Some taxa range is found extensively from sea level up to 2,500 meters above sea level. According to the IUCN Red List, Rhoiptelea chiliantha, Carya sinensis, and Pterocarya tonkinensis are threatened while Engelhardia spicata, E. serrata, Alfaropsis roxburghiana, Pterocarya stenoptera are classified as least concerned (Carya tonkinensis is threatened locally, and Engelhardia spicata var. colebrookiana and Platycarya strobilacea merit “threatened” designation in Vietnam’s Red Data Book. The most frequent threats are logging, land-use change, and habitat destruction due to artificial wood planta- tions or road construction. Until now, no conservation measures have been applied for any of the species in Vietnam, although some species occur in national parks. Our study gives an important update on the current diversity and distribution of Juglandaceae in Vietnam. We point out the need for a correct assessment of the threat status of various species on a national and international scale to protect the rarest and most endangered of them. Further research, the use of various forms of protection of individual taxa and/or their habitats, and drawing the attention of an international group of researchers to the urgent need to work together to protect biodiversity in Vietnam hot spots are necessary.
Źródło:
Dendrobiology; 2021, 86; 39-55
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Taxus baccata in Morocco: a tree in regression in its southern extreme
Autorzy:
Romo, Angel
Iszkuło, Grzegorz
Taleb, Mohammed Seghir
Walas, Łukasz
Boratyński, Adam
Powiązania:
https://bibliotekanauki.pl/articles/956808.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
dakhs
ecology
forestry
phytogeography
plant conservation
niche modelling
Opis:
The knowledge on chorology of species is basic for biogeographic and ecological studies and for conservation actions. The data on the chorology of Taxus baccata in Morocco have not been studied. The bibliographic resources, herbarium material, unpublished data of several botanists and the authors’ field notes have been reviewed. Two yew populations on Jbel Lakrâa in the Rif and near Bekrit in the Middle Atlas were measured to verify demographic structure and health conditions. The Maxent model of predicted habitat based on bioclimatic variables was used to evaluate the potential geographic range of T. baccata. The species occurs in the mountain areas of northern Morocco: in the Rif, the Middle and High Atlas, at altitudes between (500) 1200 and 2000 (2350) m. In the High Atlas, it reaches its southernmost locations within the geographic range. The populations represented by the highest number of individuals are found in the Western Rif and Middle Atlas, while from the High Atlas only single, isolated individuals are known. The yew occurred preferably in forests of Cedrus atlantica, Abies maroccana, groves of Betula fontqueri, Ilex aquifolium and Prunus lusitanica and in riparian forests located in narrow gorges. Seedlings were observed only at single localities. The population of T. baccata in the Rif presented a good health condition with successful regeneration, while the population in the Middle Atlas was in a much worse state, where only 4–8 m high yews were observed, without natural regeneration. The Maxent model of predicted habitat, based on bioclimatic variables, suggests that T. baccata could occur today in much broader mountainous areas in Morocco, especially in the Rif and Middle Atlas. However, the progressive decline of this species in Morocco indicates the significant importance of other factors on yew distribution, including microsite conditions and human pressure. Taxus baccata in Morocco is in regression at most of its localities and should be treated as a vulnerable species. Measures to ensure the yew’s conservation are needed, especially at its southernmost localities in the High Atlas.
Źródło:
Dendrobiology; 2017, 78; 63-74
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pattern of tree diversity in lowland tropical forest in Nikiwar, West Papua, Indonesia
Autorzy:
Murdjoko, A.
Djitmau, D.A.
Ungirwalu, A.
Sinery, A.S.
Siburian, R.H.S.
Mardiyadi, Z.
Wanma, A.O.
Wanma, J.F.
Rumatora, A.
Mofu, W.Y.
Worabai, D.
May, N.L.
Jitmau, M.M.
Mentansan, G.A.F.
Krey, K.
Musaad, I.
Manaf, M.
Abdullah, Y.
Mamboai, H.
Pamuji, K.E.
Raharjo, S.
Kilmaskossu, A.
Bachri, S.
Nur-Alzai, N.
Benu, N.M.H.
Tambing, J.
Kuswandi, R.
Khayati, L.
Lekitoo, K.
Powiązania:
https://bibliotekanauki.pl/articles/2078261.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
dendrogram
conservation
Importance Value Index
Shannon-Weiner
understory
Opis:
Trees are significant components of ecosystems built by several widespread species. For instance, Papua forest is known to comprise abiotic and biotic elements. Also, certain plants have grown in popu- larity to a point where they are discovered almost everywhere. The purpose of this study, therefore, was to investigate tree diversity, distribution, and the importance of conservation. Data were collected in four locations using a total of 24 sample plots spread across Idoor, Karst, Persemaian, and Torembi, where seven, four, seven, and five plots were allocated, respectively. These forests formed a mixed natural plantation comprising 76 species from 35 families. Furthermore, Idoor and Karst generated the highest species diver- sity and varied significantly compared to Persemaian, while Torembi showed similarities with the other three locations. This condition formed three ecosystem communities across Persemaian, Karst, Idoor, and Torembi. Also, the composition of the dominant species showed variations at the seedling and sapling levels believed to structure the understory, while the pole and tree levels characterized the overstory. The total species status was described as critically endangered (CR) of two species, vulnerable (VU) of six species, least con- cern (LC) of 28 species, and data deficient (DD) species. Therefore, location management is advised to not only pay significant attention in terms of economic benefits but also ecological, including the provisions for ex-situ and in-situ conservation to support sustainable forest management.
Źródło:
Dendrobiology; 2021, 85; 78-91
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modelling the potential distribution of the endemic oak Quercus vulcanica Boiss. & Heldr. ex Kotschy in Turkey from the last interglacial to the future: From near threatened to endangered
Autorzy:
Sarikaya, A.G.
Orucu, O.K.
Sen, I.
Sarikaya, O.
Powiązania:
https://bibliotekanauki.pl/articles/2130201.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
oak
biogeography
species distribution modelling
MaxEnt
biodiversity conservation
Opis:
The aims of this study are to model past (LIG, LGM and Mid-Holocene), present and future (2050 and 2070) distributions of the Quercus vulcanica Boiss. & Heldr. ex Kotschy by using Maximum Entropy, and to predict suitable areas for the conservation of the species for future planning. MaxEnt distribution modeling was used to model distributions. Results for past bioclimatic conditions show that the distribution area of the species expanded and then contracted (LIG to LGM and LGM to HOL). The modelling shows that the distribution range of the species will be narrower in the future. The species will be facing extinction towards 2070. Therefore, the conservation status of the species should be evaluated according to the present findings. Although the largest population of the Q. vulcanica is found in Isparta and Afyonkarahisar Provinces located in Southwestern Turkey, this area will not be suitable for the growth and survival of the species in the future. For this reason, a new nature reserve area should be established in a more suitable climate.
Źródło:
Dendrobiology; 2022, 88; 70-80
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chorological and conservation status of the endemic cypress, Cupressus atlantica Gaussen, in the High Atlas (Morocco)
Autorzy:
Sekiewicz, K.
Sekiewicz, M.
Romo, A.
Didukh, Y.
Fennane, M.
Boratynski, A.
Powiązania:
https://bibliotekanauki.pl/articles/41144.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
chorology
conservation status
endemic plant
cypress
Cupressus atlantica
Cupressaceae
forest ecosystem
degraded ecosystem
High Atlas Mountains
plant conservation
plant geography
Morocco
Opis:
We present a study of the distribution, ecology and conservation status of Cupressus atlantica, an endemic tree of the High Atlas (Morocco). The main populations of this species grow in a reduced area along the N’Fiss valley in the Central High Atlas and are gradually receding. Particular populations are increasingly fragmented and the total area covered by the cypress woodland has decreased to less than a third of the surface occupied in the 1930s. Overgrazing reduces the woodlands’ regenerative capacity, and the exploitation of the wood, linked to traditional uses by the rural society of the N’Fiss valley, directly reduces the number of trees. Great efforts being made to protect the species by the Haut Commissariat aux Eaux et Forêts are having some effect in recent years.
Źródło:
Dendrobiology; 2014, 71
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Conservation of genetic resources of Sorbus torminalis in Poland
Autorzy:
Bednorz, L
Powiązania:
https://bibliotekanauki.pl/articles/41249.pdf
Data publikacji:
2007
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
genetic resource
conservation
tree
Rosaceae
wild service tree
Sorbus torminalis
forest tree
Polska
Opis:
The paper presents the proposal of a strategy of conservation of genetic resources of wild service tree (Sorbus torminalis) in Polish forests. Some results of the recent studies on distribution, resources and genetic variability of wild service tree in Poland that make a scientific base of the programme are presented. Both in situ and ex situ methods should be applied in conservation of genetic resources of S. torminalis. Conservation affords should first to be focused on forest management oriented in favor of wild service tree (e.g. logging intervention, competition control, preparing of favorable sites for new establishments). Further, in situ measures should include selection of conservation areas (natural gene reserves) and preserving trees of the best quality (protected genotypes). These objects would provide seeds for new establishments and to feed populations of small size. The genotypes of the selected earlier preserving trees (about 250–300) would be protected in ex situ conservation objects – seedling and clonal seed orchards. These plantations would also provide seeds needed for future establishments. The genotypes of endangered populations or single trees of S. torminalis could be preserved in the Forest Gene Bank in Kostrzyca.
Źródło:
Dendrobiology; 2007, 58; 3-7
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution of Lihtuanian Scots pine trees according to breeding categories and their seed production in seed stands
Autorzy:
Gabrilavicius, R
Powiązania:
https://bibliotekanauki.pl/articles/41345.pdf
Data publikacji:
2002
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Scotch pine
seed stand
gene conservation
seed collection
tree
seed bearing
Lithuania
distribution
seed
Opis:
In addition to other traits, value of stands for breeding can be evaluated according to the number of plus trees and productive trees. According to this property, stands with the highest value for breeding are located in eastern Lithuania. Trees start to differentiate into breeding categories at the juvenile stage: in Scots pine stands on Pinetum myrtillosum sites, at the age of 6to 8 years and in Scots pine stands on Pinetum vacciniosum sites, at age of 10 to 12 years. According to radial increment, the trees start to differentiate into selection categories of productive, medium and minus trees, which often remain in these categories until mature age. The following four types of radial increment of trees were distinguished: (1) fast growth at the juvenile stage, followed by a gradual decrease, (2) fast growth at the juvenile stage, followed by a stable growth from age 20 to 30 years, (3) slow growth at the juvenile stage, followed gradual increase, (4) unstable growth all the time. In Lithuania, abundant seed yield used to occur in three to four year intervals. Populations with abundant flowering may produce approximately 10 kg of seeds (filled and empty) per ha, while the populations with weak flowering - 1 to 2 kg per ha. In mature Scots pine stands of stocking level ca 0.7, 6to 8% of the seeds are produced by plus trees, 24% by productive trees, 55 to 60% by trees of medium productivity and 10% by minus trees. Being suppressed, minus trees produce 2% of the seeds only.
Źródło:
Dendrobiology; 2002, 47 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of North African Thymus algeriensis in Tunisia: Population structure and implication for conservation
Autorzy:
Ben El Hadj Ali, I.
Guetat, A.
Boussaid, M.
Powiązania:
https://bibliotekanauki.pl/articles/41188.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
genetic diversity
North Africa
Thymus algeriensis
Tunisia
population structure
molecular marker
natural population
conservation
Opis:
The genetic diversity within and among nine natural populations of Thymus hirtus Willd. subsp. algeriensis (Boiss. et Reut.) Murb. from different geographical and bioclimatic zones were assessed using Random Amplified Polymorphic DNA data. A total of 154 bands were generated from seven selected primers. 141 bands were polymorphic (P=91.56%). The genetic diversity within a population based on Shannon's index which was high (H'pop=0.307) and varied according to bioclimatic zones. A high genetic differentiation among populations (GST=0.335 and FST=0.296) was revealed, suggesting a population isolation and a low level of gene flow among them. The major proportion of the variation was attributable to individual differences within populations. The genetic structure is in accordance with geography distances. The Neighbour- joining tree based on Nei's and Li's genetic distance among individuals showed that individuals from each population clustered together. The UPGMA dendrogram based on FST values revealed three clusters each of them includes populations closuring to their geographical origin. The high genetic structure of populations resulted from genetic drift caused both by the habitat fragmentation and the low size of most populations. The in situ conservation should interest all populations. The ex situ conservation should be based on the collection of seeds rather within than among populations because of the maximum of variation was revealed within populations.
Źródło:
Dendrobiology; 2012, 67
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An assessment of genetic diversity following natural regeneration of Sorbus torminalis in the Forest District of Jamy (northern Poland)
Autorzy:
Bednorz, L.
Myczko, L.
Kosinski, P.
Powiązania:
https://bibliotekanauki.pl/articles/41322.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
assessment
genetic diversity
natural regeneration
Sorbus torminalis
conservation
wild service tree
Forest District Jamy
Polska
Opis:
The subject of the study was a population of Sorbus torminalis resulting from spontaneous regeneration from seeds in northern Poland at the north-eastern limit of the species distribution. The population, occupying a fenced plot of 1.72 ha in the Forest District of Jamy, amounted to 579 individuals of variable age classes up to about 19 years old. Six polymorphic gene loci: MDH-B, ME-A, 6PGD-B, ADH-B, PGM-A, PGM-B were studied in 532 individuals recruited post-fencing and 21 potentially parental, adult trees. The analysis showed similarly high levels of genetic diversity within both subsets of individuals but there was also a noticeable change in genetic structure between them. Detailed genetic analysis of the post-fencing recruits revealed lack of significant differences between different age groups based on DBH within the fenced plot.
Źródło:
Dendrobiology; 2015, 73
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of postglacial relict shrub Betula nana revealed by RAPD analysis
Autorzy:
Dabrowska, G
Dzialuk, A.
Burnicka, O.
Ejankowski, W.
Gugnacka-Fiedor, W.
Goc, A.
Powiązania:
https://bibliotekanauki.pl/articles/41579.pdf
Data publikacji:
2006
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Betula nana
genetic diversity
genetic resource
conservation
population genetics
postglacial relict shrub
random amplified polymorphic DNA
Opis:
A sample of Betula nana from the Linie reserve near Dąbrowa Chełmińska, have been fingerprinted using random amplified polymorphic DNA (RAPD). The high level of genetic variation was detected. All individuals had unique genotypes, supporting the generally high resolving power of RAPD’s. For the conservation strategy, information about the distribution of the genetic variation within and among populations plays very important role. Thus, extensive study in other populations of dwarf birch is needed.
Źródło:
Dendrobiology; 2006, 55; 19-23
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of wild Thymus capitatus (Lamiaceae) in Tunisia using molecular markers
Autorzy:
Ben El Hadj Ali, I.
Guetat, A.
Boussaid, M.
Powiązania:
https://bibliotekanauki.pl/articles/41663.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
genetic diversity
wild plant
Thymus capitatus
Lamiaceae
Tunisia
molecular marker
habitat fragmentation
conservation strategy
genetic structure
Opis:
In Tunisia, Thymus capitatus L. populations are severely destroyed due to deforestation and over-collecting. The species occurs in small scattered populations decreasing progressively in size. Yet, no conservation or improvement programs are attempted to preserve and promote the potential value of this resource. In this work, we assessed the genetic diversity of nine Tunisian populations of Thymus capitatus L. from different bioclimates, using 103 polymorphic randomly amplified polymorphic DNA (RAPD) loci. The analysis of the genetic variation within and among populations is primordial to elaborate conservation and improvement programs. The species showed a low diversity within populations (0.276
Źródło:
Dendrobiology; 2012, 68
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Salix myrtilloides in north central Poland. Distribution, threats and conservation
Autorzy:
Churski, M
Danielewicz, W.
Powiązania:
https://bibliotekanauki.pl/articles/41057.pdf
Data publikacji:
2008
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
glacial relict
willow
Polska
locality
habitat loss
Salix myrtilloides
distribution
threat
conservation
habitat protection
gene pool
strategy
Opis:
Present status of a postglacial relict – Salix myrtilloides L. was investigated in north central Poland at 27 localities. Consequently 3 sites were confirmed, 4 were not confirmed, and the remaining 20 were considered to be extinct. In comparison to the historical distribution, ca. 74% of the sites existing 50 years ago do not exist today in the studied region. The confirmed populations were found near Oporówek (ca. 1600 stems), Rakowiec (ca. 100) and Bobrowo (15). At three sites confirmed in this study, the existence of vigorous hybrids between S. myrtilloides and S. aurita L. (S. ×onusta Besser) were observed. The most important threats to Salix myrtilloides L. populations in the studied region were assessed to be hybridization with other commonly occurring willow species, site isolation and habitat destruction. Natural habitat protection with adequate buffer zone and gene pool protection in a form of plantations was suggested as a conservation strategy. As an alternative strategy active protection ‘insitu’ was proposed based on cutting competitive common species of willows.
Źródło:
Dendrobiology; 2008, 60; 3-9
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro callus and shoot organogenesis from leaf and stem explants of Chamaedaphne calyculata
Autorzy:
Zrobek-Sokolnik, A.
Dynowski, P.
Holdynski, C.
Powiązania:
https://bibliotekanauki.pl/articles/41437.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
in vitro culture
callus
shoot
organogenesis
leaf
stem
explant
Chamaedaphne calyculata
Ericaceae
micropropagation
plant species
plant conservation
Opis:
Leatherleaf Chamaedaphne calyculata (L) Moench is a relict, rare and endangered species in Poland. There are no reports on the micropropagation of Chamaedaphne calyculata in the literature. Therefore, the aim of this study was to propose a propagation protocol for leatherleaf via indirect organogenesis using leaves and stems (internodal segments) derived from mature plants growing in a natural stand and from plants grown in vitro as explants. The medium developed by Anthony et al. (2004) with 100%, 50% and 25% salt concentrations, supplemented with IAA (5 and 10μM) and TDZ (5 and 10μM), was used for callus development and the induction of adventitious shoots. The media developed by Anthony et al. (2004) and Anderson (1980), both containing 10μM TDZ and 5μM IAA or 2.28μM zeatin, were used for adventitious shoot elongation. Secondary explants proved to be the most effective starting material for callus induction, the regeneration and elongation of adventitious shoots. The most supportive medium for callus induction and growth and the induction of adventitious shoots was the full medium proposed by Anthony et al. (2004) containing 5μM IAA and 10μM TDZ. Anderson’s (1980) medium containing 2.28μM zeatin delivered optimal results in the elongation of adventitious shoots of Ch. calyculata. Roots were cultivated on Anderson’s (1980) phytohormone-free medium. Approximately 65% of the plantlets survived after transfer to the sphagnum-peat and perlite mixture (3:1). The plants grew normally without any signs of morphological variation. This study makes the first ever attempt to propose an effective micropropagation protocol for Ch. calyculata.
Źródło:
Dendrobiology; 2014, 72
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 26

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