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    Tytuł:
    Similarity of chromosome structure among Populus tremula var. davidiana, Populus alba and their hybrids revealed by FISH karyotype analysis
    Autorzy:
    Kim, Y.G.
    Kwon, S.H.
    Kang, H.I.
    Yoem, D.B.
    Kim, K.W.
    Kim, H.H.
    Kang, K.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2077655.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Karyotype analysis
    Fluorescence in situ hybridization
    Ribosomal DNA site
    Poplar
    Cytogenetics
    Opis:
    The genus Populus is one of the important tree species in Korean peninsula and many other coun- tries in the world. It represents the model species of forest genomics because it grows fast and reproduces rapidly. In this reason, their genetic characteristics have been well studied and the whole genome has been sequenced completely in some species. However, cytogenetic study of the genus Populus has been limited. In the present study, karyotypes of Korean aspen (P. tremula var. davidiana), Silver poplar (P. alba) and their two hybrids, Suwon aspen (P. tremula var. glandulosa) and Hyun aspen (P. alba × P. tremula var. glandulsa) were analyzed by means of the fluorescence in situ hybridization (FISH). Root samples were collected from mature trees in the demonstration forest, located at Suwon, Kyonggi province in South Korea. The fresh root cells were examined by DAPI (4’,6-diamidino-2-phenylindole) staining and FISH using 45S rDNA and 5S rDNA probes. As the results, the chromosome compositions of all species were the same as 2n = 38. The karyotype formulas of Korean aspen, Silver poplar, Suwon aspen and Hyun aspen were 28m + 6sm + 4st (2sat), 26m + 10sm (2sat) + 2st, 26m + 12sm (2sat) and 28m + 10sm (2sat), respectively. The four species had one pair of 45S rDNA site and one pair of 5S rDNA site in common with FISH karyotypes. The similarity of FISH karyotypes among four species indicated close genetic relationship and coexistence of their interspecific hybrids. This research will provide genetic information on cytogenetic research of Populus and genetic mapping that can be applied to the breeding program of Populus in the near future.
    Źródło:
    Dendrobiology; 2020, 83; 68-74
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Morphology of strobili at different development positions and cone and seed characteristics of Pinus densiflora f. multicaulis
    Autorzy:
    Kwon, S.H.
    Kim, Y.G.
    Kang, H.I.
    Shim, D.
    Kang, K.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2078259.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    bisexual strobili
    sex reversal
    microsporangia
    megasporangia
    cone analysis
    Opis:
    Pinus densiflora f. multicaulis is a rare and unusual form of genus Pinus. It has excellent ornamen- tal values due to many trunks from its root collar, and produces female, male, and bisexual strobili on the same tree. However, there is limited study whether the bisexual strobili are functional, mature lateral cones produce filled seeds, and the seeds from the mature lateral cones germinate and grow as well as those from mature apical cones. The structure of apical female, and lateral male and bisexual strobili were analyzed in an open-pollination-progeny population. The strobili were photographed to obtain external, cross-section, and radial-section images using a 45× stereoscopic microscope. Radial sections of strobili were imaged using scanning electron microscopy. Characteristics of apical and lateral cones and seeds were surveyed by cone analysis and germination test. P. densiflora f. multicaulis developed typical female strobili apically, and ordinary male and bisexual strobili laterally. Additionally, female strobili developed laterally whereas the male strobili were normally located. The mature lateral female strobili had the same structure as the apical female. In the mature bisexual strobili, the proportion of male and female tissue was unequal. The cones developed from lateral female strobili showed highly significant differences in the length, width, and weight, being shorter (32.7%), narrower (26.9%), and lighter (59.8%) than those from apical female strobili. Both types produced filled seeds and seedlings, and well significant positive correlations were found among characteristics of seeds from apical and lateral female strobili. The initial seed germination rate of the seeds from apical female strobili was higher than that of seeds from lateral female strobili, but the difference decreased over time. The viability and spatial distribution of the female and male tissue of bisporangiate cones, and their frequent occurrence suggests a common mechanism for the production of bisporangiate structures in seed plants.
    Źródło:
    Dendrobiology; 2021, 85; 51-59
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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